LOCUS       sequence-462401-        4597 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AiE0140h is an enhancer sequence, designed to drive AAV-mediated 
            transgene expression in specific populations of brain cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4597)
  AUTHORS   Ben-Simon Y, Hooper M, Narayan S, Daigle TL, Dwivedi D, Way SW, 
            Oster A, Stafford DA, Mich JK, Taormina MJ, Martinez RA, Opitz-Araya
            X, Roth JR, Alexander JR, Allen S, Amster A, Arbuckle J, Ayala A, 
            Baker PM, Bakken TE, Barcelli T, Barta S, Bendrick J, Bertagnolli D,
            Bielstein C, Bishwakarma P, Bowlus J, Boyer G, Brouner K, Casian B, 
            Casper T, Chakka AB, Chakrabarty R, Chance RK, Chavan S, Clark M, 
            Colbert K, Collman F, Daniel S, Departee M, DiValentin P, Donadio N,
            Dotson N, Egdorf T, Fliss T, Gabitto M, Garcia J, Gary A, Gasperini 
            M, Gloe J, Goldy J, Gore BB, Graybuck L, Greisman N, Haeseleer F, 
            Halterman C, Haradon Z, Hastings SD, Helback O, Ho W, Hockemeyer D, 
            Huang C, Huff S, Hunker A, Johansen N, Jones D, Juneau Z, Kalmbach 
            B, Kannan M, Khem S, Kussick E, Kutsal R, Larsen R, Lee C, Lee AY, 
            Leibly M, Lenz GH, Li S, Liang E, Lusk N, Madigan Z, Malloy J, 
            Malone J, McCue R, Melchor J, Mollenkopf T, Moosman S, Morin E, 
            Newman D, Ng L, Ngo K, Omstead V, Otto S, Oyama A, Pena N, Pham T, 
            Phillips E, Pom CA, Potekhina L, Ransford S, Ray PL, Rette D, 
            Reynoldson C, Rimorin C, Rocha D, Ruiz A, Sanchez REA, Sawyer L, 
            Sedeno-Cortes A, Sevigny JP, Shapovalova N, Shepard N, Shulga L, 
            Sigler AR, Siverts L, Soliman S, Somasundaram S, Staats B, Stewart 
            K, Szelenyi E, Tieu M, Trader C, Tran A, van Velthoven CTJ, Walker 
            M, Wang Y, Weed N, Wirthlin M, Wood T, Wynalda B, Yao Z, Zhou T, 
            Ariza J, Dee N, Reding M, Ronellenfitch K, Mufti S, Sunkin SM, Smith
            KA, Esposito L, Waters J, Thyagarajan B, Yao S, Lein ES, Zeng H, 
            Levi BP, Ngai J, Ting JT, Tasic B
  TITLE     A suite of enhancer AAVs and transgenic mouse lines for genetic 
            access to cortical cell types.
  JOURNAL   Cell. 2025 May 16:S0092-8674(25)00513-6. doi: 
            10.1016/j.cell.2025.05.002.
  PUBMED    40403729
REFERENCE   2  (bases 1 to 4597)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            May 16:S0092-8674(25)00513-6. doi: 10.1016/j.cell.2025.05.002."
COMMENT     Sequence Label: AiP13786 - pAAV-AiE0140h-minBG-iCre(R297T)-BGH...
FEATURES             Location/Qualifiers
     source          1..4597
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      533..542
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             545..565
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1631..1742
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   1860..2000
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   1860..1989
                     /label=AAV2 ITR
     rep_origin      2075..2530
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2162..2181)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2372..2393
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2547..2566)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2666..2688
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2726..2744)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2812..2916
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2917..2985,2986..3777)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3135..3154)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3948..4536
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4437..4456
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtggttgt gtgtgaagat taagcccttt cttgctctat
      181 gtatttgagc ctagaaaaag acacactccc aagaaattag aataaaggga aaaatgactt
      241 tgagatgtac tttcttaaaa taactgaagt aatgaccgca tgagccagct gatgggtttt
      301 taaatggata cgtttctatc agcctgctgc ttgatgccag agccaaatat atatggagtg
      361 tattatatca tatcctgtag gcaggagact gttggggcat tgggggactt agagaggtga
      421 agtggcaggc ttggcacagg aattaacagc agagctcggg ctgggcataa aagtcagggc
      481 agagccatct attgcttaca tttgcttctg ggatccagat ctttcgaagc tagccaccat
      541 ggtgcccaag aagaagagga aagtctccaa cctgctgact gtgcaccaaa acctgcctgc
      601 cctccctgtg gatgccacct ctgatgaagt caggaagaac ctgatggaca tgttcaggga
      661 caggcaggcc ttctctgaac acacctggaa gatgctcctg tctgtgtgca gatcctgggc
      721 tgcctggtgc aagctgaaca acaggaaatg gttccctgct gaacctgagg atgtgaggga
      781 ctacctcctg tacctgcaag ccagaggcct ggctgtgaag accatccaac agcacctggg
      841 ccagctcaac atgctgcaca ggagatctgg cctgcctcgc ccttctgact ccaatgctgt
      901 gtccctggtg atgaggagaa tcagaaagga gaatgtggat gctggggaga gagccaagca
      961 ggccctggcc tttgaacgca ctgactttga ccaagtcaga tccctgatgg agaactctga
     1021 cagatgccag gacatcagga acctggcctt cctgggcatt gcctacaaca ccctgctgcg
     1081 cattgccgaa attgccagaa tcagagtgaa ggacatctcc cgcaccgatg gtgggagaat
     1141 gctgatccac attggcagga ccaagaccct ggtgtccaca gctggtgtgg agaaggccct
     1201 gtccctgggg gttaccaagc tggtggagag atggatctct gtgtctggtg tggctgatga
     1261 ccccaacaac tacctgttct gccgggtcag aaagaatggt gtggctgccc cttctgccac
     1321 ctcccaactg tccacccggg ccctggaagg gatctttgag gccacccacc gcctgatcta
     1381 tggtgccaag gatgactctg ggcagagata cctggcctgg tctggccact ctgccagagt
     1441 gggtgctgcc accgacatgg ccagggctgg tgtgtccatc cctgaaatca tgcaggctgg
     1501 tggctggacc aatgtgaaca ttgtgatgaa ctacatcaga aacctggact ctgagactgg
     1561 ggccatggtg aggctgctcg aagatgggga ctgaggcgcg ccgaattcaa gcttctcgag
     1621 agatcttcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     1681 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     1741 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     1801 ggggaggatt gggaagacaa tagcaggcat gagatctcac gtgcggaccg agcggccgca
     1861 ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc
     1921 cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg
     1981 agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     2041 tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc
     2101 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cttagcgccc
     2161 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     2221 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     2281 aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     2341 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     2401 ctcaactcta tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggtctat
     2461 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     2521 tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     2581 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     2641 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     2701 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     2761 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     2821 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     2881 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     2941 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     3001 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     3061 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     3121 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     3181 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     3241 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     3301 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     3361 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     3421 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     3481 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     3541 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     3601 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     3661 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     3721 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     3781 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     3841 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     3901 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     3961 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     4021 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     4081 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     4141 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     4201 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     4261 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     4321 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     4381 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     4441 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     4501 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     4561 cggttcctgg ccttttgctg gccttttgct cacatgt
//
