LOCUS       sequence-462406-        4721 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AiE0128h is an enhancer sequence, designed to drive AAV-mediated 
            transgene expression in specific populations of brain cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4721)
  AUTHORS   Ben-Simon Y, Hooper M, Narayan S, Daigle TL, Dwivedi D, Way SW, 
            Oster A, Stafford DA, Mich JK, Taormina MJ, Martinez RA, Opitz-Araya
            X, Roth JR, Alexander JR, Allen S, Amster A, Arbuckle J, Ayala A, 
            Baker PM, Bakken TE, Barcelli T, Barta S, Bendrick J, Bertagnolli D,
            Bielstein C, Bishwakarma P, Bowlus J, Boyer G, Brouner K, Casian B, 
            Casper T, Chakka AB, Chakrabarty R, Chance RK, Chavan S, Clark M, 
            Colbert K, Collman F, Daniel S, Departee M, DiValentin P, Donadio N,
            Dotson N, Egdorf T, Fliss T, Gabitto M, Garcia J, Gary A, Gasperini 
            M, Gloe J, Goldy J, Gore BB, Graybuck L, Greisman N, Haeseleer F, 
            Halterman C, Haradon Z, Hastings SD, Helback O, Ho W, Hockemeyer D, 
            Huang C, Huff S, Hunker A, Johansen N, Jones D, Juneau Z, Kalmbach 
            B, Kannan M, Khem S, Kussick E, Kutsal R, Larsen R, Lee C, Lee AY, 
            Leibly M, Lenz GH, Li S, Liang E, Lusk N, Madigan Z, Malloy J, 
            Malone J, McCue R, Melchor J, Mollenkopf T, Moosman S, Morin E, 
            Newman D, Ng L, Ngo K, Omstead V, Otto S, Oyama A, Pena N, Pham T, 
            Phillips E, Pom CA, Potekhina L, Ransford S, Ray PL, Rette D, 
            Reynoldson C, Rimorin C, Rocha D, Ruiz A, Sanchez REA, Sawyer L, 
            Sedeno-Cortes A, Sevigny JP, Shapovalova N, Shepard N, Shulga L, 
            Sigler AR, Siverts L, Soliman S, Somasundaram S, Staats B, Stewart 
            K, Szelenyi E, Tieu M, Trader C, Tran A, van Velthoven CTJ, Walker 
            M, Wang Y, Weed N, Wirthlin M, Wood T, Wynalda B, Yao Z, Zhou T, 
            Ariza J, Dee N, Reding M, Ronellenfitch K, Mufti S, Sunkin SM, Smith
            KA, Esposito L, Waters J, Thyagarajan B, Yao S, Lein ES, Zeng H, 
            Levi BP, Ngai J, Ting JT, Tasic B
  TITLE     A suite of enhancer AAVs and transgenic mouse lines for genetic 
            access to cortical cell types.
  JOURNAL   Cell. 2025 May 16:S0092-8674(25)00513-6. doi: 
            10.1016/j.cell.2025.05.002.
  PUBMED    40403729
REFERENCE   2  (bases 1 to 4721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            May 16:S0092-8674(25)00513-6. doi: 10.1016/j.cell.2025.05.002."
COMMENT     Sequence Label: AiP14764 - pAAV-AiE0128h-minBG-iCre(R297T)-BGH...
FEATURES             Location/Qualifiers
     source          1..4721
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      657..666
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             669..689
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1755..1866
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   1984..2124
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   1984..2113
                     /label=AAV2 ITR
     rep_origin      2199..2654
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2286..2305)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2496..2517
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2671..2690)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2790..2812
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2850..2868)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2936..3040
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3041..3109,3110..3901)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3259..3278)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4072..4660
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4561..4580
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtggtcta gtatcatgag gaggatctca aagcagggaa
      181 gggaagccag ctgagggacc cctgagagga gatcagggga taaatacttt gcaacagaat
      241 ccccatggga aacatgacaa aattagtaac aggaatggag cctccctcag aggatacatg
      301 aatcgttgag ttgaaaataa ccacaaggtc atcaaatcca gctgccacca actgcctgaa
      361 tattttcaac aaatttttga acaagcagtc ctccagcctc ctccagctgc atccagcagc
      421 tcagtgctcc acctgacagc ctcttctgat agcgtgaagg ctcagaaagc ccctgtgggg
      481 cccagcacat cctggggcag gcctctagtg ggcagagggc agcatactgg ggtctggatc
      541 taacacactc ctccatccag gtggtatatt tataggagct cgggctgggc ataaaagtca
      601 gggcagagcc atctattgct tacatttgct tctgggatcc agatctttcg aagctagcca
      661 ccatggtgcc caagaagaag aggaaagtct ccaacctgct gactgtgcac caaaacctgc
      721 ctgccctccc tgtggatgcc acctctgatg aagtcaggaa gaacctgatg gacatgttca
      781 gggacaggca ggccttctct gaacacacct ggaagatgct cctgtctgtg tgcagatcct
      841 gggctgcctg gtgcaagctg aacaacagga aatggttccc tgctgaacct gaggatgtga
      901 gggactacct cctgtacctg caagccagag gcctggctgt gaagaccatc caacagcacc
      961 tgggccagct caacatgctg cacaggagat ctggcctgcc tcgcccttct gactccaatg
     1021 ctgtgtccct ggtgatgagg agaatcagaa aggagaatgt ggatgctggg gagagagcca
     1081 agcaggccct ggcctttgaa cgcactgact ttgaccaagt cagatccctg atggagaact
     1141 ctgacagatg ccaggacatc aggaacctgg ccttcctggg cattgcctac aacaccctgc
     1201 tgcgcattgc cgaaattgcc agaatcagag tgaaggacat ctcccgcacc gatggtggga
     1261 gaatgctgat ccacattggc aggaccaaga ccctggtgtc cacagctggt gtggagaagg
     1321 ccctgtccct gggggttacc aagctggtgg agagatggat ctctgtgtct ggtgtggctg
     1381 atgaccccaa caactacctg ttctgccggg tcagaaagaa tggtgtggct gccccttctg
     1441 ccacctccca actgtccacc cgggccctgg aagggatctt tgaggccacc caccgcctga
     1501 tctatggtgc caaggatgac tctgggcaga gatacctggc ctggtctggc cactctgcca
     1561 gagtgggtgc tgccaccgac atggccaggg ctggtgtgtc catccctgaa atcatgcagg
     1621 ctggtggctg gaccaatgtg aacattgtga tgaactacat cagaaacctg gactctgaga
     1681 ctggggccat ggtgaggctg ctcgaagatg gggactgagg cgcgccgaat tcaagcttct
     1741 cgagagatct tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     1801 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     1861 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     1921 caagggggag gattgggaag acaatagcag gcatgagatc tcacgtgcgg accgagcggc
     1981 cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg
     2041 aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg
     2101 agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt
     2161 gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt
     2221 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccttagc
     2281 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     2341 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     2401 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     2461 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     2521 aacactcaac tctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggt
     2581 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     2641 aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     2701 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     2761 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     2821 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     2881 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     2941 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     3001 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     3061 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     3121 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     3181 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     3241 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     3301 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     3361 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     3421 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     3481 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     3541 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     3601 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     3661 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     3721 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     3781 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     3841 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     3901 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     3961 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     4021 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     4081 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     4141 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     4201 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     4261 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     4321 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     4381 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     4441 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     4501 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     4561 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     4621 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     4681 tttacggttc ctggcctttt gctggccttt tgctcacatg t
//
