LOCUS       sequence-462429-        1158 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of N-terminally 6His tagged A-kinase anchor 
            protein 8 LCD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1158)
  TITLE     Mittag bacterial expression plasmids
REFERENCE   2  (bases 1 to 1158)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDest17_AKAP8 LCD (1-367)
FEATURES             Location/Qualifiers
     source          1..1158
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             20..40
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 gtacaaaaaa gcaggctccg aaaacctgta tttccagggc agcatggatc agggttatgg
       61 tggttatggc gcctggagtg ccggtccggc caatacccag ggtgcctatg gcaccggcgt
      121 tgccagctgg cagggctatg aaaattataa ttattacggt gcacagaaca ccagtgttac
      181 caccggtgca acctatagct atggcccggc aagctgggaa gccgccaaag caaatgatgg
      241 tggtctggcc gcaggtgccc cggctatgca tatggcaagc tatggtccgg aaccgtgcac
      301 cgataatagt gatagcctga ttgcaaaaat taaccagcgc ctggatatga tgagcaaaga
      361 aggtggtcgt ggcggcagcg gcggtggtgg tgaaggtatt caggatcgtg aaagcagttt
      421 tcgttttcag ccgtttgaaa gctatgatag ccgcccgtgt ctgccggaac ataatccgta
      481 tcgcccgagc tatagctatg attatgaatt tgatctgggc agcgatcgta atggtagttt
      541 tggtggccag tatagcgaat gccgtgatcc ggcccgcgaa cgtggcagcc tggatggttt
      601 tatgcgtggc cgtggccagg gccgttttca ggatcgcagt aatccgggta cctttatgcg
      661 cagcgatccg tttgtgccgc cggcagcaag tagcgaaccg ctgagcaccc cgtggaatga
      721 actgaattat gttggtggtc gcggcctggg tggcccgtca cctagtcgtc cgccgcctag
      781 cctgtttagt cagagcatgg caccggatta tggtgttatg ggcatgcagg gtgcaggcgg
      841 ttatgatagc accatgccgt atggctgcgg tcgtagtcag ccgcgtatgc gcgatcgtga
      901 tcgtccgaaa cgccgcggct ttgatcgctt tggtccggat ggtaccggcc gtaaacgcaa
      961 acagtttcag ctgtatgaag aaccggatac caaactggcc cgcgttgata gtgaaggcga
     1021 ttttagcgaa aatgatgatg cagcaggcga ttttcgtagt ggcgatgaag aattcaaagg
     1081 cgaagatgaa ctgtgcgata gtggccgcca gcgcggtgaa aaagaagatg aagatgaaga
     1141 cgtttaaaac ccagcttt
//
