LOCUS       sequence-46250-e         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   de Marco A, Deuerling E, Mogk A, Tomoyasu T, Bukau B
  TITLE     Chaperone-based procedure to increase yields of soluble recombinant 
            proteins produced in E. coli.
  JOURNAL   BMC Biotechnol. 2007 Jun 12;7:32. doi: 10.1186/1472-6750-7-32.
  PUBMED    17565681
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2007-06-12"; volume: "7:32. doi"; pages: " 
            10.1186/1472-6750-7-32"
COMMENT     Sequence Label: pBB550
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(23..109)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tcgactaggt ctagggcggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
       61 agataaaacg aaaggcccag tctttcgact gagcctttcg ttttatttga tgcctctaga
      121 tcagctaatt aagcttggct gcaggtcgac atcttagcgg gtcaggtcgt caaaaaactt
      181 cttcacacca tcaaagaagc tctttgagcg cgggctgttg tgctcgccgg ttgggccacc
      241 gaagctttct tgcagctctt gcagcagctg tttctgcctt tcgttcaggc ctaccggtgt
      301 ttcgacgaca acgcggcaca gcaaatcacc ctgtgcgcca ccgcggacag acttgacgcc
      361 tttaccgcgc atacggaata gcttaccggt ctgggtttcg ccaggcactt tcagtttgac
      421 gcgaccatca agggtcggta cttcgatttc gccacccagc gccgccatag cgaagttgat
      481 cgggacttcg caatacaggt tgttgccttc acgctcgaaa atcgggtgct gtttaacctg
      541 aacctgaacg tacagatcgc ctgccggtgc gccatgctcg cccgcttcac cttcgcccgc
      601 aagacggatg cggtctccag tgtccacccc tgccgggatt ttaacggaca gcgttttgct
      661 gcgctcaaca cgaccatgac catgacattt gttgcacgga tctttgatca gcgtaccgcg
      721 gccctgacag tgtggacagg tctgctgtac agcgaagaat ccctggcgca tctgcacctg
      781 accagaacca tgacaggtcg gacaagtctg cggctgtgta cctgg
//
