LOCUS       sequence-462513-        4960 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AiE2366m is an enhancer sequence, designed to induce cre-dependent 
            recombination in specific populations of brain cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4960)
  AUTHORS   Ben-Simon Y, Hooper M, Narayan S, Daigle TL, Dwivedi D, Way SW, 
            Oster A, Stafford DA, Mich JK, Taormina MJ, Martinez RA, Opitz-Araya
            X, Roth JR, Alexander JR, Allen S, Amster A, Arbuckle J, Ayala A, 
            Baker PM, Bakken TE, Barcelli T, Barta S, Bendrick J, Bertagnolli D,
            Bielstein C, Bishwakarma P, Bowlus J, Boyer G, Brouner K, Casian B, 
            Casper T, Chakka AB, Chakrabarty R, Chance RK, Chavan S, Clark M, 
            Colbert K, Collman F, Daniel S, Departee M, DiValentin P, Donadio N,
            Dotson N, Egdorf T, Fliss T, Gabitto M, Garcia J, Gary A, Gasperini 
            M, Gloe J, Goldy J, Gore BB, Graybuck L, Greisman N, Haeseleer F, 
            Halterman C, Haradon Z, Hastings SD, Helback O, Ho W, Hockemeyer D, 
            Huang C, Huff S, Hunker A, Johansen N, Jones D, Juneau Z, Kalmbach 
            B, Kannan M, Khem S, Kussick E, Kutsal R, Larsen R, Lee C, Lee AY, 
            Leibly M, Lenz GH, Li S, Liang E, Lusk N, Madigan Z, Malloy J, 
            Malone J, McCue R, Melchor J, Mollenkopf T, Moosman S, Morin E, 
            Newman D, Ng L, Ngo K, Omstead V, Otto S, Oyama A, Pena N, Pham T, 
            Phillips E, Pom CA, Potekhina L, Ransford S, Ray PL, Rette D, 
            Reynoldson C, Rimorin C, Rocha D, Ruiz A, Sanchez REA, Sawyer L, 
            Sedeno-Cortes A, Sevigny JP, Shapovalova N, Shepard N, Shulga L, 
            Sigler AR, Siverts L, Soliman S, Somasundaram S, Staats B, Stewart 
            K, Szelenyi E, Tieu M, Trader C, Tran A, van Velthoven CTJ, Walker 
            M, Wang Y, Weed N, Wirthlin M, Wood T, Wynalda B, Yao Z, Zhou T, 
            Ariza J, Dee N, Reding M, Ronellenfitch K, Mufti S, Sunkin SM, Smith
            KA, Esposito L, Waters J, Thyagarajan B, Yao S, Lein ES, Zeng H, 
            Levi BP, Ngai J, Ting JT, Tasic B
  TITLE     A suite of enhancer AAVs and transgenic mouse lines for genetic 
            access to cortical cell types.
  JOURNAL   Cell. 2025 May 16:S0092-8674(25)00513-6. doi: 
            10.1016/j.cell.2025.05.002.
  PUBMED    40403729
REFERENCE   2  (bases 1 to 4960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            May 16:S0092-8674(25)00513-6. doi: 10.1016/j.cell.2025.05.002."
COMMENT     Sequence Label: AiP20068 - pAAV-AiE2366m-minBG-iCre(R297T)-BGHpA
FEATURES             Location/Qualifiers
     source          1..4960
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      896..905
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             908..928
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1994..2105
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2223..2363
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2223..2352
                     /label=AAV2 ITR
     rep_origin      2438..2893
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2525..2544)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2735..2756
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2910..2929)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3029..3051
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3089..3107)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3175..3279
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3280..3348,3349..4140)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3498..3517)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4311..4899
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4800..4819
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttgtaag agtaataatc gacttacaaa cacaactttt
      181 aaaccgtttt gagccattca taaagtactt aagacaaaca caaagtcaga gcctctcaac
      241 actttgtgtg tgaactatac ggtacaaagt ttagacttta tttgaaaact acttgctttc
      301 tgcctaataa tcaggatgaa gtaattagag atgcagaagt atttaaatct tgtttgcttc
      361 agaacattgt ttatcttccc aaacacctgt tttatcacca catcatgcac agctcatctg
      421 tcctcatttg tagctttcat tatcttcagt cttttagcag cggctgacct gtatgcgcat
      481 ttcaactaaa tctgtatgat ccaagaatat ggtacaacag acgatgtaga tgtatatttt
      541 tatactctga tggcaggcat tataaaataa cagaggaaga gacatataat tattttcctt
      601 tctccaaact cattcttcac tataaatatt cagacttgga ggtttattat ttgaataaat
      661 gttcaaaata attagatata atgatctagc aataagtata aaattaaagc cctaagtatt
      721 cagttctgca agaataatca tccgcttgag aacagccttt tcaagtcact aattgcacat
      781 tcttacttat taaacgatca ttagtattct ttcggagctc gggctgggca taaaagtcag
      841 ggcagagcca tctattgctt acatttgctt ctgggatcca gatctttcga agctagccac
      901 catggtgccc aagaagaaga ggaaagtctc caacctgctg actgtgcacc aaaacctgcc
      961 tgccctccct gtggatgcca cctctgatga agtcaggaag aacctgatgg acatgttcag
     1021 ggacaggcag gccttctctg aacacacctg gaagatgctc ctgtctgtgt gcagatcctg
     1081 ggctgcctgg tgcaagctga acaacaggaa atggttccct gctgaacctg aggatgtgag
     1141 ggactacctc ctgtacctgc aagccagagg cctggctgtg aagaccatcc aacagcacct
     1201 gggccagctc aacatgctgc acaggagatc tggcctgcct cgcccttctg actccaatgc
     1261 tgtgtccctg gtgatgagga gaatcagaaa ggagaatgtg gatgctgggg agagagccaa
     1321 gcaggccctg gcctttgaac gcactgactt tgaccaagtc agatccctga tggagaactc
     1381 tgacagatgc caggacatca ggaacctggc cttcctgggc attgcctaca acaccctgct
     1441 gcgcattgcc gaaattgcca gaatcagagt gaaggacatc tcccgcaccg atggtgggag
     1501 aatgctgatc cacattggca ggaccaagac cctggtgtcc acagctggtg tggagaaggc
     1561 cctgtccctg ggggttacca agctggtgga gagatggatc tctgtgtctg gtgtggctga
     1621 tgaccccaac aactacctgt tctgccgggt cagaaagaat ggtgtggctg ccccttctgc
     1681 cacctcccaa ctgtccaccc gggccctgga agggatcttt gaggccaccc accgcctgat
     1741 ctatggtgcc aaggatgact ctgggcagag atacctggcc tggtctggcc actctgccag
     1801 agtgggtgct gccaccgaca tggccagggc tggtgtgtcc atccctgaaa tcatgcaggc
     1861 tggtggctgg accaatgtga acattgtgat gaactacatc agaaacctgg actctgagac
     1921 tggggccatg gtgaggctgc tcgaagatgg ggactgaggc gcgccgaatt caagcttctc
     1981 gagagatctt cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     2041 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     2101 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     2161 aagggggagg attgggaaga caatagcagg catgagatct cacgtgcgga ccgagcggcc
     2221 gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga
     2281 ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga
     2341 gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg
     2401 cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta
     2461 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccttagcg
     2521 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     2581 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     2641 aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     2701 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     2761 acactcaact ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggtc
     2821 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     2881 acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     2941 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     3001 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     3061 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     3121 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     3181 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     3241 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     3301 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     3361 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     3421 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     3481 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     3541 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     3601 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     3661 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     3721 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     3781 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     3841 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     3901 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     3961 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     4021 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     4081 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     4141 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     4201 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     4261 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     4321 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     4381 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     4441 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     4501 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     4561 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     4621 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     4681 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     4741 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     4801 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     4861 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     4921 ttacggttcc tggccttttg ctggcctttt gctcacatgt
//
