LOCUS       sequence-46254-e         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  TITLE     Lees Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Lox-Stop-Lox TOPO Hygro
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(33..66)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tctgatgcat gctcgagcgg ccgctggtcg acataacttc gtatagcata cattatacga
       61 agttatctcg accagcttct gatggaatta gaacttggca aaacaatact gagaatgaag
      121 tgtatgtgga acagaggctg ctgatctcgt tcttcaggct atgaaactga cacatttgga
      181 aaccacagta cttagaacca caaagtggga atcaagagaa aaacaatgat cccacgagag
      241 atctatagat ctatagatca tgagtgggag gaatgagctg gcccttaatt tggttttgct
      301 tgtttaaatt atgatatcca actatgaaac attatcataa agcaatagta aagagccttc
      361 agtaaagagc aggcatttat ctaatcccac cccaccccca cccccgtagc tccaatcctt
      421 ccattcaaaa tgtaggtact ctgttctcac ccttcttaac aaagtatgac aggaaaaact
      481 tccattttag tggacatctt tattgtttaa tagatcatca atttctgcat cccggggatc
      541 tgatatcatc gatactctat tcctttgccc tcggacgagt gctggggcgt cggtttccac
      601 tatcggcgag tacttctaca cagccatcgg tccagacggc cgcgcttctg cgggcgattt
      661 gtgtacgccc gacagtcccg gctccggatc ggacgattgc gtcgcatcga ccctgcgccc
      721 aagctgcatc atcgaaattg ccgtcaacca agctctgata gagttggtca agaccaatgc
      781 ggagcatata cgcccggagc cgcggcgatc ctgcaagctc cggatgcc
//
