LOCUS       sequence-462554-        1188 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Quantitative bacterial two-hybrid (qB2H). Expresses Asf1A..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Guyot A, Maillard E, Ferreira-Pinto K, Plancon-Arnould L, 
            Nadaradjane AA, Ochsenbein F, Guerois R, Martin L, Ramos OHP
  TITLE     Optimized quantitative bacterial two-hybrid (qB2H) for 
            protein-protein interaction assessment
  JOURNAL   bioRxiv 2025.10.29.680610
REFERENCE   2  (bases 1 to 1188)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.10.29.680610"
COMMENT     Sequence Label: VN550 pAC-cI_E34P-Asf1A_Term_f1-pOL2-62_L-eGFP...
FEATURES             Location/Qualifiers
     source          1..1188
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..711
                     /codon_start=1
                     /gene="cI"
                     /product="phage lambda repressor"
                     /label=lambda repressor
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQPSVADKMGMGQ
                     SGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
ORIGIN
        1 atgagcacaa aaaagaaacc attaacacaa gagcagcttg aggacgcacg tcgccttaaa
       61 gcaatttatg aaaaaaagaa aaatgaactt ggcttatccc agccgtctgt cgcagacaag
      121 atggggatgg ggcagtcagg cgttggtgct ttatttaatg gcatcaatgc attaaatgct
      181 tataacgccg cattgcttgc aaaaattctc aaagttagcg ttgaagaatt tagcccttca
      241 atcgccagag aaatctacga gatgtatgaa gcggttagta tgcagccgtc acttagaagt
      301 gagtatgagt accctgtttt ttctcatgtt caggcaggga tgttctcacc tgagcttaga
      361 acctttacca aaggtgatgc ggagagatgg gtaagcacaa ccaaaaaagc cagtgattct
      421 gcattctggc ttgaggttga aggtaattcc atgaccgcac caacaggctc caagccaagc
      481 tttcctgacg gaatgttaat tctcgttgac cctgagcagg ctgttgagcc aggtgatttc
      541 tgcatagcca gacttggggg tgatgagttt accttcaaga aactgatcag ggatagcggt
      601 caggtgtttt tacaaccact aaacccacag tacccaatga tcccatgcaa tgagagttgt
      661 tccgttgtgg ggaaagttat cgctagtcag tggcctgaag agacgtttgg cgcggccgca
      721 gcaaaggttc aggtgaacaa tgtagtggtg ctggataacc cttctccttt ctacaacccg
      781 ttccagttcg agatcacctt cgagtgcatc gaggacctgt ctgaagactt ggaatggaaa
      841 attatctatg tgggctctgc agaaagtgaa gaatacgatc aagttttaga ctctgtttta
      901 gtgggtcctg ttcccgcagg aaggcatatg tttgtatttc aggctgatgc acctaatcca
      961 ggactcattc cagatgcaga tgcagtaggc gtaactgttg tgctaattac ttgtacctat
     1021 cgaggacaag aatttattag agttggctat tatgtaaata atgaatatac tgagacagaa
     1081 ttaagggaaa atccaccagt aaaaccagac ttttctaagc ttcaaaggaa tattttggca
     1141 tctaatccca gggtcacaag attccacatt aattgggaag ataactga
//
