LOCUS       sequence-462557-        1564 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Guyot A, Maillard E, Ferreira-Pinto K, Plancon-Arnould L, 
            Nadaradjane AA, Ochsenbein F, Guerois R, Martin L, Ramos OHP
  TITLE     Optimized quantitative bacterial two-hybrid (qB2H) for 
            protein-protein interaction assessment
  JOURNAL   bioRxiv 2025.10.29.680610
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.10.29.680610"
COMMENT     Sequence Label: VN627 pAC-cI_E34P-Asf1B_Term_f1-pOL2-62_L_eGFP...
FEATURES             Location/Qualifiers
     source          1..1564
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..3,4..6,7..717)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             749..1564
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             749..1564
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(822..841)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 atggtcagta aaggcgaaga gttgtttacg ggtgtagtac cgattttagt ggaactcgat
       61 ggagatgtga atgggcataa attttcggtt tcaggtgaag gtgaaggaga tgccacctat
      121 ggcaaactga cgctgaagtt catctgtacg acaggcaaac tgcctgttcc gtggccaaca
      181 ctggttacta cgctgaccta tggcgttcaa tgcttttcgc gttatccgga ccacatgaaa
      241 cagcatgact tctttaaatc cgcgatgcct gaaggttacg tacaagaacg caccatcttc
      301 ttcaaagatg acgggaacta caaaacccgt gcagaggtca aatttgaggg tgacaccctt
      361 gtgaatcgga ttgagctgaa aggcattgac tttaaagaag atggcaacat tctgggtcat
      421 aaactggagt acaactacaa cagccataac gtgtatatca tggcggacaa acagaagaat
      481 gggatcaaag tgaatttcaa gattcgccac aacattgaag atggtagtgt gcagttagcc
      541 gaccactatc agcagaatac cccgattggc gatggcccag tcttgctgcc ggataatcat
      601 taccttagca cccaatctgc gttaagcaaa gatcccaacg aaaagcgcga tcacatggtc
      661 ctcctggaat ttgtgactgc tgcaggcatc actttgggta tggatgaact gtataagtaa
      721 acgcgttaaa caggaaacag aagtccaaat gagccatatt caacgggaaa cgtcttgctc
      781 taggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga
      841 taatgtcggg caatcaggtg cgacaatcta tcgattgtat gggaagcccg atgcgccaga
      901 gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag
      961 actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc
     1021 tgatgatgca tggttactca ccactgcgat ccccgggaaa acagcattcc aggtattaga
     1081 agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt
     1141 gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca
     1201 ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa
     1261 tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga
     1321 ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt
     1381 aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat
     1441 cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata
     1501 tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt
     1561 ctaa
//
