LOCUS       sequence-462581-         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Guyot A, Maillard E, Ferreira-Pinto K, Plancon-Arnould L, 
            Nadaradjane AA, Ochsenbein F, Guerois R, Martin L, Ramos OHP
  TITLE     Optimized quantitative bacterial two-hybrid (qB2H) for 
            protein-protein interaction assessment
  JOURNAL   bioRxiv 2025.10.29.680610
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.10.29.680610"
COMMENT     Sequence Label: VN1263
            pB2Hws_v28_pLWeakRBS_TIR371_cI-Asf1B+rp...
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgcagggtt ctgtgacaga gtttctaaaa ccgcgcctgg ttgatatcga gcaagtgagt
       61 tcgacgcacg ccaaggtgac ccttgagcct ttagagcgtg gctttggcca tactctgggt
      121 aacgcactgc gccgtattct gctctcatcg atgccgggtt gcgcggtgac cgaggttgag
      181 attgatggtg tactacatga gtacagcacc aaagaaggcg ttcaggaaga tatcctggaa
      241 atcctgctca acctgaaagg gctggcggtg agagttcagg gcaaagatga agttattctt
      301 accttgaata aatctggcat tggccctgtg actgcagccg atatcaccca cgacggtgat
      361 gtcgaaatcg tcaagccgca gcacgtgatc tgccacctga ccgatgagaa cgcgtctatt
      421 agcatgcgta tcaaagttca gcgcggtcgt ggttatgtgc cggcttctac ccgaattcat
      481 tcggaagaag atgagcgccc aatcggccgt ctgctggtcg acgcatgcta cagccctgtg
      541 gagcgtattg cctacaatgt tgaagcagcg cgtgtagaac agcgtaccga cctggacaag
      601 ctggtcatcg aaatggaaac caacggcaca atcgatcctg aagaggcgat tcgtcgtgcg
      661 gcaaccattc tggctgaaca actggaagct ttcgttgact tacgtgatgt acgtcagcct
      721 gaagtgaaag aagagaaacc agaggcggcc gcaggcgcga tgggcaccat catgccgaaa
      781 gatatccagc tggcgcgtcg tatccgtggc tga
//
