LOCUS       sequence-462588-        1188 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B. In fusion
            cloning to rpoA using BsaI based golden gate..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Guyot A, Maillard E, Ferreira-Pinto K, Plancon-Arnould L, 
            Nadaradjane AA, Ochsenbein F, Guerois R, Martin L, Ramos OHP
  TITLE     Optimized quantitative bacterial two-hybrid (qB2H) for 
            protein-protein interaction assessment
  JOURNAL   bioRxiv 2025.10.29.680610
REFERENCE   2  (bases 1 to 1188)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.10.29.680610"
COMMENT     Sequence Label: VN1897
            pB2Hws_v28_Ddcm_wkKanR_TIR51_pLWeakRBS_...
FEATURES             Location/Qualifiers
     source          1..1188
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..711
                     /codon_start=1
                     /gene="cI"
                     /product="phage lambda repressor"
                     /label=lambda repressor
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQPSVADKMGMGQ
                     SGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
ORIGIN
        1 atgagcacaa aaaagaaacc attaacacaa gagcagcttg aggacgcacg tcgccttaaa
       61 gcaatttatg aaaaaaagaa aaatgaactt ggcttatccc agccgtctgt cgcagacaag
      121 atggggatgg ggcagtcagg cgttggtgct ttatttaatg gcatcaatgc attaaatgct
      181 tataacgccg cattgcttgc aaaaattctc aaagttagcg ttgaagaatt tagcccttca
      241 atcgccagag aaatctacga gatgtatgaa gcggttagta tgcagccgtc acttagaagt
      301 gagtatgagt accctgtttt ttctcatgtt caggcaggga tgttctcacc tgagcttaga
      361 acctttacca aaggtgatgc ggagagatgg gtaagcacaa ccaaaaaagc cagtgattct
      421 gcattctggc ttgaggttga aggtaattcc atgaccgcac caacaggctc caagccaagc
      481 tttcctgacg gaatgttaat tctcgttgac cctgagcagg ctgttgagcc aggtgatttc
      541 tgcatagcca gacttggggg tgatgagttt accttcaaga aactgatcag ggatagcggt
      601 caggtgtttt tacaaccact aaacccacag tacccaatga tcccatgcaa tgagagttgt
      661 tccgttgtgg ggaaagttat cgctagtcag tggcctgaag agacgtttgg cggtaccgca
      721 atggcgaaag tctcggtgct gaatgtggca gtgctggaaa atccgtcccc gttccatagc
      781 ccgttccgct ttgaaatctc gtttgaatgt tccgaagcgc tggccgatga cctggaatgg
      841 aaaattatct atgtgggcag cgcggaatct gaagaatttg atcagatcct ggactcagtc
      901 ctggtgggcc cggtcccggc aggtcgtcat atgtttgttt tccaagcaga tgctccgaac
      961 ccgtcgctga ttccggaaac cgacgcagtt ggtgtcacgg tggttctgat tacctgcacg
     1021 tatcacggcc aggaattcat ccgcgtgggt tattacgtta acaatgaata cctgaacccg
     1081 gaactgcgtg aaaatccgcc gatgaaaccg gattttagtc agctgcaacg caacattctg
     1141 gcatccaatc cgcgtgttac ccgcttccat atcaattggg ataactga
//
