LOCUS       sequence-46265-e         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Kwon GS, Hadjantonakis AK
  TITLE     Transthyretin mouse transgenes direct RFP expression or Cre-mediated
            recombination throughout the visceral endoderm.
  JOURNAL   Genesis. 2009 Jul;47(7):447-55.
  PUBMED    19415627
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genesis."; 
            date: "2009-07"; volume: "47(7)"; pages: "447-55"
COMMENT     Sequence Label: Ttr:mRFP1
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             169..201
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(205..213,214..228)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             235..912
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             235..912
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(365..383)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     633..652
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     827..850
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gctcattaat tgtcaacact tatgttatca taatgggatc agcatgtact tttggttttg
       61 ttccagagtc tatcaccgga aagaacaagc cggtttactc tgacccattt cactgacatt
      121 tctcttgtct cctctgtgcc cagggtgctg gagaggaatt cgccaccaat ggctagcatg
      181 actggtggac agcaaatggg tcgggatctg tacgacgatg acgataagga tccgatggcc
      241 tcctccgagg acgtcatcaa ggagttcatg cgcttcaagg tgcgcatgga gggctccgtg
      301 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
      361 accgccaagc tgaaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc
      421 cctcagttcc agtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac
      481 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
      541 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg
      601 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc
      661 tgggaggcct ccaccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag
      721 atgaggctga agctgaagga cggcggccac tacgacgccg aggtcaagac cacctacatg
      781 gccaagaagc ccgtgcagct gcccggcgcc tacaagaccg acatcaagct ggacatcacc
      841 tcccacaacg aggactacac catcgtggaa cagtacgagc gcgccgaggg ccgccactcc
      901 accggcgcct aa
//
