LOCUS       sequence-46268-e         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Kim do J, Kim O, Kim HW, Kim HS, Lee SJ, Suh SW
  TITLE     ATP-dependent DNA ligase from Archaeoglobus fulgidus displays a 
            tightly closed conformation.
  JOURNAL   Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Jun 1. 65(Pt 
            6):544-50.
  PUBMED    19478428
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Acta 
            Crystallogr Sect F Struct Biol Cryst Commun. 2009 Jun 1. 65(Pt 
            6):544-50."
COMMENT     Sequence Label: pET28b-AF0623
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggcc tggtgccgcg cggcagccat atgatgttgt ttgccgagtt tgccgagttc
      121 tgcgaaaggc tcgaaaaaat atcgtcaaca cttgagctta ccgcgaggat agctgcgttt
      181 cttcaaaaaa ttgaggatga gagagacctt tatgacgttg tcctttttat caccggcaag
      241 gtttacccgc cgtgggatga gagggagctt ggtgtcggga ttggattgct ctacgaggct
      301 cttgaaaacg taagcggagt caagagaagc gagatagaga gtatgataag ggagtacgga
      361 gacctcggtc tcgttgcgga gcagctgata aagaagaaga aaatgacaac cctcgccttc
      421 gaggagctta cagtgaggaa agtgagggag accttcgatg agatcgcatc tttgacggga
      481 gaagggagca tgaaaagaaa gattatgctc ctcaccggcc tctacggtct cgcaactcca
      541 ctagaggcga ggtaccttac aaggctgatt ttgaatgaga tgcgccttgg tgttggagag
      601 ggcataatga gggatgctat agccagagct ttcagggcgg atccggaaac ggttgagagg
      661 gcttacatga tcaccaacga cctcggcagg gtggcagtgg ttgccaagaa agagggcgag
      721 gagggcttga ggaaaatgaa gatagagatt cacattccgg tcagaatgat gctcgcacag
      781 gttgctgaga gcctcgaaag cgcagttaga gagatgagaa cagcg
//
