LOCUS       sequence-462689-        9934 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  New version of a PVX vector to express heterologous sequences in 
            plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9934)
  AUTHORS   Uranga M, Aragones V, Garcia A, Mirabel S, Gianoglio S, Presa S, 
            Granell A, Pasin F, Daros JA
  TITLE     RNA virus-mediated gene editing for tomato trait breeding.
  JOURNAL   Hortic Res. 2023 Dec 19;11(1):uhad279. doi: 10.1093/hr/uhad279. 
            eCollection 2024 Jan.
  PUBMED    38895601
REFERENCE   2  (bases 1 to 9934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hortic 
            Res."; date: "2023-12-19"; volume: "11(1):uhad279. doi"; pages: " 
            10.1093/hr/uhad279. eCollection 2024 Jan"
COMMENT     Sequence Label: pLBPVXBa-M
FEATURES             Location/Qualifiers
     source          1..9934
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     248..268
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        257..302
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     regulatory      1010..1019
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(7213..8028)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8020..8039
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             complement(8154..8816)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      8817..9588
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     misc_feature    9808..9832
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     misc_feature    9859..9883
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gattccattg cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct
       61 cctacaaatg ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca
      121 gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa
      181 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
      241 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga
      301 gggaaaacta aaccatacac caccaacaca accaaaccca ccacgcccaa ttgttacaca
      361 cccgcttgaa aaagaaagtt taacaaatgg ccaaggtgcg cgaggtttac caatctttta
      421 cagactccac cacaaaaact ctcatccaag atgaggctta tagaaacatt cgccccatca
      481 tggaaaaaca caaactagct aacccttacg ctcaaacggt tgaagcggct aatgatctag
      541 aggggttcgg catagccacc aatccctata gcattgaatt gcatacacat gcagccgcta
      601 agaccataga gaataaactt ctagaggtgc ttggttccat cctaccacaa gaacctgtta
      661 catttatgtt tcttaaaccc agaaagctaa actacatgag aagaaacccg cggatcaagg
      721 acattttcca aaatgttgcc attgaaccaa gagacgtagc caggtacccc aaggaaacaa
      781 taattgacaa actcacagag atcacaacgg aaacagcata cattagtgac actctgcact
      841 tcttggatcc gagctacata gtggagacat tccaaaactg cccaaaattg caaacattgt
      901 atgcgacctt agttctcccc gttgaggcag cctttaaaat ggaaagcact cacccgaaca
      961 tatacagcct caaatacttc ggagatggtt tccagtatat accaggcaac catggtggcg
     1021 gggcatacca tcatgaattc gctcatctac aatggctcaa agtgggaaag atcaagtgga
     1081 gggaccccaa ggatagcttt ctcggacatc tcaattacac gactgagcag gttgagatgc
     1141 acacagtgac agtacagttg caggaatcgt tcgcggcaaa ccacttgtac tgcatcagga
     1201 gaggagactt gctcacaccg gaggtgcgca ctttcggcca acctgacagg tacgtgattc
     1261 caccacagat cttcctccca aaagttcaca actgcaagaa gccgattctc aagaaaacta
     1321 tgatgcagct cttcttgtat gttaggacag tcaaggtcgc aaaaaattgt gacatttttg
     1381 ccaaagtcag acaattaatt aaatcatctg acttggacaa atactctgct gtggaactgg
     1441 tttacttagt aagctacatg gagttccttg ccgatttaca agctaccacc tgcttctcag
     1501 acacactttc tggtggcttg ctaacaaaga cccttgcacc ggtgagggct tggatacaag
     1561 agaaaaagat gcagctgttt ggtcttgagg actacgcgaa gttagtcaaa gcagttgatt
     1621 tccacccggt ggatttttct ttcaaagtgg aaacttggga cttcagattc caccccttgc
     1681 aagcgtggaa agccttccga ccaagggaag tgtcggatgt agaggaaatg gaaagtttgt
     1741 tctcagatgg ggacttgctt gattgcttca caagaatgcc agcttatgcg gtaaacgcag
     1801 aggaagattt agctgcaatc aggaaaacgc ccgagatgga tgtcggtcaa gaagttaaag
     1861 aacctgcagg agacagaaat caatactcaa accctgcaga aactttcctc aacaagctcc
     1921 acaggaaaca cagtagggag gtgaaacacc aggccgcaaa gaaagctaaa cgcctagctg
     1981 aaatccagga gtcaatgaga gctgaaggtg atgccgaacc aaatgaaata agcgggggca
     2041 tgggggcaat acccagcaac gccgaacttc ctggcacgaa tgatgccaga caagaactca
     2101 cactcccaac cactaaacct gtccctgcaa ggtgggaaga tgcttcattc acagattcta
     2161 gtgtggaaga ggagcaggtt aaaccccttg gaaaagaaac cgttgaaaca gcgacgcaac
     2221 aagtcatcga aggacttcct tggaaacact ggattcctca attaaatgct gttggattca
     2281 aggcgctgga aattcagagg gataggagtg gaacaatgat catgcccatc acagaaatgg
     2341 tctccgggct ggaaaaagag gacttccctg aaggaactcc aaaagagttg gcacgagaat
     2401 tgttcgctat gaacagaagc cctgccacca tccctttgga cctgcttaga gccagagact
     2461 acggcagtga tgtaaagaac aagagaattg gtgccatcac aaagacacag gcaacgagtt
     2521 ggggcgaata cttgacagga aagatagaaa gcttgactga gaggaaagtt gcgacttgtg
     2581 tcattcatgg agctggaggt tctggaaaaa gtcatgccat ccagaaggca ttgagagaaa
     2641 ttggcaaggg ctcggacatc actgtagtcc tgccgaccaa tgaactgcgg ctagattgga
     2701 gtaagaaagt gcctaacact gagccctata tgttcaagac ctatgaaaag gcgttaattg
     2761 ggggaacagg cagcatagtc atctttgacg attactcaaa acttcctccc ggttacatag
     2821 aagccttagt ctgtttctac tctaaaatca agctaatcat tctaacagga gatagcagac
     2881 aaagcgtcta ccatgaaact gctgaggacg cctccatcag gcatttggga ccagcaacag
     2941 agtacttctc aaaatactgc cgatactatc tcaatgccac acaccgcaac aagaaagatc
     3001 ttgcgaacat gcttggtgtc tacagtgaga gaacgggagt caccgaaatc agcatgagcg
     3061 ccgagttctt agaaggaatc ccaactttgg taccctcgga tgagaagaga aagctgtaca
     3121 tgggcaccgg gaggaatgac acgttcacat acgctggatg ccaggggcta actaagccga
     3181 aggtacaaat agtgttggac cacaacaccc aagtgtgtag cgcgaatgtg atgtacacgg
     3241 cactttctag agccaccgat aggattcact tcgtgaacac aagtgcaaat tcctcggcct
     3301 tctgggaaaa gttggacagc accccttacc tcaagacttt cctatcagtg gtgagagaac
     3361 aagcactcag ggagtacgag ccggcagagg cagagccaat tcaagagcct gagccccaga
     3421 cacacatgtg tgtcgagaat gaggagtccg tgctagaaga gtacaaagag gaactcttgg
     3481 aaaagtttga cagagagatc cactctgaat cccatggtca ttcaaactgt gtccaaactg
     3541 aagacacaac cattcagttg ttttcgcatc aacaagcaaa agatgagacc ctcctctggg
     3601 cgactataga tgcgcggctc aagaccagca atcaagagac aaacttccga gaattcctga
     3661 gcaagaagga cattggggac gttctgtttt taaactacca aaaagctatg ggtttaccca
     3721 aagagcgtat tcctttttcc caagaggtct gggaagcttg tgcccacgaa gtacaaagca
     3781 agtacctcag caagtcaaag tgcaacttga tcaatgggac tgtgagacag agcccagact
     3841 tcgatgaaaa taagattatg gtattcctca agtcgcagtg ggtcacaaag gtggaaaaac
     3901 taggtctacc caagattaag ccaggtcaaa ccatagcagc cttttaccag cagactgtga
     3961 tgctttttgg aactatggct aggtacatgc gatggttcag acaggctttc cagccaaaag
     4021 aagtcttcat aaactgtgag accacgccag atgacatgtc tgcatgggcc ttgaacaact
     4081 ggaatttcag cagacctagc ttggctaatg actacacagc tttcgaccag tctcaggatg
     4141 gagccatgtt gcaatttgag gtgctcaaag ccaaacacca ctgcatacca gaggaaatca
     4201 ttcaggcata catagatatt aagactaatg cacagatttt cctaggcacg ttatcaatta
     4261 tgcgcctgac tggtgaaggt cccacttttg atgcaaacac tgagtgcaac atagcttaca
     4321 cccatacaaa gtttgacatc ccagccggaa ctgctcaagt ttatgcagga gacgactccg
     4381 cactggactg tgttccagaa gtgaagcata gtttccacag gcttgaggac aaattactcc
     4441 taaagtcaaa gcctgtaatc acgcagcaaa agaagggcag ttggcctgag ttttgtggtt
     4501 ggctgatcac accaaaaggg gtgatgaaag acccaattaa gctccatgtt agcttaaaat
     4561 tggctgaagc taagggtgaa ctcaagaaat gtcaagattc ctatgaaatt gatctgagtt
     4621 atgcctatga ccacaaggac tctctgcatg acttgttcga tgagaaacag tgtcaggcac
     4681 acacactcac ttgcagaaca ctaatcaagt cagggagagg cactgtctca ctttcccgcc
     4741 tcagaaactt tctttaaccg ttaagttacc ttagagattt gaataagatg gatattctca
     4801 tcagtagttt gaaaagttta ggttattcta ggacttccaa atctttagat tcaggacctt
     4861 tggtagtaca tgcagtagcc ggagccggta agtccacagc cctaaggaag ttgatcctca
     4921 gacacccaac attcaccgtg catacactcg gtgtccctga caaggtgagt atcagaacta
     4981 gaggcataca gaagccagga cctattcctg agggcaactt cgcaatcctc gatgagtata
     5041 ctttggacaa caccacaagg aactcatacc aggcactttt tgctgaccct tatcaggcac
     5101 cggagtttag cctagagccc cacttctact tggaaacatc atttcgagtt ccgaggaaag
     5161 tggcagattt gatagctggc tgtggcttcg atttcgagac caactcaccg gaagaagggc
     5221 acttagagat cactggcata ttcaaagggc ccctactcgg aaaggtgata gccattgatg
     5281 aggagtctga gacaacactg tccaggcatg gtgttgagtt tgttaagccc tgccaagtga
     5341 cgggacttga gttcaaagta gtcactattg tgtctgccgc accaatagag gaaattggcc
     5401 agtccacagc tttctacaac gctatcacca ggtcaaaggg attgacatat gtccgcgcag
     5461 ggccataggc tgaccgctcc ggtcaattct gaaaaagtgt acatagtatt aggtctatca
     5521 tttgctttag tttcaattac ctttctgctt tctagaaata gcttacccca cgtcggtgac
     5581 aacattcaca gcttgccaca cggaggagct tacagagacg gcaccaaagc aatcttgtac
     5641 aactccccaa atctagggtc acgagtgagt ctacacaacg gaaagaacgc agcatttgct
     5701 gccgttttgc tactgacttt gctgatctat ggaagtaaat acatatctca acgcaatcat
     5761 acttgtgctt gtggtaacaa tcatagcagt cattagcact tccttagtga ggactgaacc
     5821 ttgtgtcatc aagattactg gggaatcaat cacagtgttg gcttgcaaac tagatgcaga
     5881 aaccataagg gccattgccg atctcaagcc actctccgtt gaacggttaa gtttccattg
     5941 atactcgaaa gaggtcagca ccagctagca cgcgtagggt ttgttaagtt tcccttttta
     6001 ctcgaaagat gtcaggcctg ttcactatcc cggatgggga tttctttagt acagcccgtg
     6061 ccatagtagc cagcaatgct gtcgcaacaa atgaggacct cagcaagatt gaggctattt
     6121 ggaaggacat gaaggtgccc acagacacta tggcacaggc tgcttgggac ttagtcagac
     6181 actgtgctga tgtaggatca tccgctcaaa cagaaatgat agatacaggt ccctattcca
     6241 acggcatcag cagagctaga ctggcagcag caattaaaga ggtgtgcaca cttaggcaat
     6301 tttgcatgaa gtatgcccca gtggtatgga actggatgtt aactaacaac agtccacctg
     6361 ctaactggca agcacaaggt ttcaagcctg agcacaaatt cgctgcattc gacttcttca
     6421 atggagtcac caacccagct gccatcatgc ccaaagaggg gctcatccgg ccaccgtctg
     6481 aagctgaaat gaatgctgcc caaactgctg cctttgtgaa gattacaaag gccagggcac
     6541 aatccaacga ctttgccagc ctagatgcag ctgtcactcg aggtcgtatc actggaacaa
     6601 caaccgctga ggctgttgtc actctaccac caccataact acgtctacat aaccgacgcc
     6661 taccccagtt tcatagtatt ttctggtttg attgtatgaa taatataaat aaaaaaaaaa
     6721 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaacgctgaa
     6781 atcaccagtc tctctctaca aatctatctc tctctatttt ctccataaat aatgtgtgag
     6841 tagtttcccg ataagggaaa ttagggttct tatagggttt cgctcatgtg ttgagcatat
     6901 aagaaaccct tagtatgtat ttgtatttgt aaaatacttc tatcaataaa atttctaatt
     6961 cctaaaacca aaatccaggt caggagacgg gacaaggatg cgagctagcc tgcaggaatt
     7021 gttgattttg tgacgactga tggcaggata tatgcggttg taattcattt ttattgtcta
     7081 aatttctgta tttgtttgtt tgttcggttg taaatttttt tggaagaaca agaaaagaaa
     7141 aaacacccgt tagggtgttt ttagttagtg tggcgcgccg acttgcgaca tgcggtcctt
     7201 tgcaatcaac tattagaaaa attcatccag catcagatga aattgcagtt tgttcatatc
     7261 cggattatca atgccatatt tctgaaacag acgtttttgc aggctcgggc taaattcgcc
     7321 caggcagttc cacagaatgg ccagatcctg ataacgatcc gcaatgccca cacggcccac
     7381 atcaatgcag ccaatcagtt tgccttcatc gaaaatcagg ttatccaggc taaaatcgcc
     7441 gtgggtcacc acgctatccg ggctaaacgg cagcagttta tgcatttctt tccacacctg
     7501 ttccaccggc cagccgttac gttcatcatc aaaatcgctc gcatccacca ggccgttgtt
     7561 catacggctc tgcgcctggg ccagacgaaa cacacgatcg ctgttaaacg ggcagttgca
     7621 caccggaatg ctatgcagac gacgcagaaa cacggccagc gcatccacaa tgttttcgcc
     7681 gctatccgga tattcttcca gcacctgaaa cgcggttttg cccggaatcg cggtggtcag
     7741 cagccacgca tcatccgggg tgcgaataaa atgtttaatg gtcggcagcg gcataaattc
     7801 ggtcagccag ttcagacgca ccatttcatc ggtcacatcg ttcgccacgc tgcctttgcc
     7861 atgtttcaga aacagttccg gcgcatccgg tttgccatac agacgataaa tggtcgcgcc
     7921 gctctgaccc acgttatcac gcgcccattt atagccatac agatccgcat ccatgttgct
     7981 gttcagacgc ggacggctac agctcgtttc acgctgaata tggctcataa caccccttgt
     8041 attactgttt atgtaagcag acagttttat tgttcatgat gatatatttt tatcttgtgc
     8101 aatgtaacat cagagatttt gagacacaag atcggattgg cggttatgcg gttctaccgg
     8161 cgcggcagcg ttacccgtgt cggcggctcc aacggctcgc catcgtccag aaaacacggc
     8221 tcatcgggca tcggcaggcg ctgctgcccg cgccgttccc attcctccgt ttcggtcaag
     8281 gctggcaggt ctggttccat gcccggaatg ccgggctggc tgggcggctc ctcgccgggg
     8341 ccggtcggta gttgctgctc gcccggatac agggtcggga tgcggcgcag gtcgccatgc
     8401 cccaacagcg attcgtcctg gtcgtcgtga tcaaccacca cggcggcact gaacaccgac
     8461 aggcgcaact ggtcgcgggg ctggccccac gccacgcggt cattgaccac gtaggccgac
     8521 acggtgccgg ggccgttgag cttcacgacg gagatccagc gctcggccac caagtccttg
     8581 actgcgtatt ggaccgtccg caaagaacgt ccgatgagct tggaaagtgt cttctggctg
     8641 accaccacgg cgttctggtg gcccatctgc gccacgaggt gatgcagcag cattgccgcc
     8701 gtgggtttcc tcgcaataag cccggcccac gcctcatgcg ctttgcgttc cgtttgcacc
     8761 cagtgaccgg gcttgttctt ggcttgaatg ccgatttctc tggactgcgt ggccatgctt
     8821 atctccatgc ggtaggggtg ccgcacggtt gcggcaccat gcgcaatcag ctgcaacttt
     8881 tcggcagcgc gacaacaatt atgcgttgcg taaaagtggc agtcaattac agattttctt
     8941 taacctacgc aatgagctat tgcggggggt gccgcaatga gctgttgcgt accccccttt
     9001 tttaagttgt tgatttttaa gtctttcgca tttcgcccta tatctagttc tttggtgccc
     9061 aaagaagggc acccctgcgg ggttccccca cgccttcggc gcggctcccc ctccggcaaa
     9121 aagtggcccc tccggggctt gttgatcgac tgcgcggcct tcggccttgc ccaaggtggc
     9181 gctgccccct tggaaccccc gcactcgccg ccgtgaggct cggggggcag gcgggcgggc
     9241 ttcgcccttc gactgccccc actcgcatag gcttgggtcg ttccaggcgc gtcaaggcca
     9301 agccgctgcg cggtcgctgc gcgagccttg acccgccttc cacttggtgt ccaaccggca
     9361 agcgaagcgc gcaggccgca ggccggaggc ttttccccag agaaaattaa aaaaattgat
     9421 ggggcaaggc cgcaggccgc gcagttggag ccggtgggta tgtggtcgaa ggctgggtag
     9481 ccggtgggca atccctgtgg tcaagctcgt gggcaggcgc agcctgtcca tcagcttgtc
     9541 cagcagggtt gtccacgggc cgagcgaagc gagccagccg gtggccgctc gcggccatcg
     9601 tccacatatc cacgggctgg caagggagcg cagcgaccgc gcagggcgaa gcccggagag
     9661 caagcccgta gggggccatc atcagttcgg tggtcttccg acgaacaata aggccgcaaa
     9721 tcgcggcctt ttttattgat aacaaaaccg gctcagttct gcgtagaaac caacatgcaa
     9781 gctccaccgg gtgcaaagcg gcagcggcgg caggatatat tcaattgtaa atggcttcat
     9841 gtccgggaaa tctactggtg gcaggatata ttgtggtgta aacaatggag aaaaagatta
     9901 attaagtact agtagcctgg tcgaaaccgt ctca
//
