LOCUS       sequence-462693-       11296 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses components of the LOOMINA optogenetic transcriptional 
            control system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11296)
  AUTHORS   Muench P, Fiumara M, Southern N, Coda D, Aschenbrenner S, Correia B,
            Graff J, Niopek D, Mathony J
  TITLE     A modular toolbox for the optogenetic deactivation of transcription.
  JOURNAL   Nucleic Acids Res. 2024 Dec 16:gkae1237. doi: 10.1093/nar/gkae1237.
  PUBMED    39676667
REFERENCE   2  (bases 1 to 11296)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2024 Dec 16:gkae1237. doi: 10.1093/nar/gkae1237."
COMMENT     Sequence Label: Zdk3-VPR
FEATURES             Location/Qualifiers
     source          1..11296
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(17..36)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        208..587
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        589..787
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     742..762
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             805..985
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1032..1157
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1650..1883
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2068..2112
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2410..2527
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2652..3830
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2883..3821
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3778..3798
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3837..3846
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3855..3875
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4137..4286
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             4755..5111
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             5130..5699
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     CDS             5721..5741
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    5794..6382
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5847..5867)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6265..6276)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6385..6401)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6386..6402)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6907..7087
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7113..7130)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7119..7343
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7553..7981
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7640..7659)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7850..7871
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7990..8010)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7995..8324
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8175..8310
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8237..8256
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8372..8419
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8438..8812
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8942..9063
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8979..8998)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9033..9052
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9112..9128)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9112..9128)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9125..9147)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9136..9152
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9160..9166,9167..9184,9185..9190))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9205..9226
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9343..9360)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9514..10102)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9594..9613)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10273..11064,11065..11133))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10896..10915
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11134..11238)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc
       61 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
      121 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc
      181 gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat taatagtaat
      241 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
      301 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
      361 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
      421 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
      481 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
      541 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
      601 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
      661 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
      721 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
      781 taagcagcgc gttttgcctg tactgggtct ctctggttag accagatctg agcctgggag
      841 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
      901 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
      961 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1021 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1081 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1141 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1201 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1261 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1321 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1381 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1441 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1501 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     1561 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     1621 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     1681 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     1741 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     1801 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     1861 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     1921 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     1981 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2041 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2101 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2161 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2221 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2281 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     2341 attcgattag tgaacggatc ggcactgcgt gcgccaattc tgcagacaaa tggcagtatt
     2401 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     2461 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     2521 aaattttcgg gtttattaca gggacagcag agatccagtt tggttaatta atgcaaagat
     2581 ggataaagtt ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag
     2641 gagtgggaat tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg
     2701 agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa
     2761 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt
     2821 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac
     2881 aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg
     2941 tgccttgaat tacttccacc tggctgcagt acgtgattct tgatcccgag cttcgggttg
     3001 gaagtgggtg ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag
     3061 ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct
     3121 gtctcgctgc tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc
     3181 tttttttctg gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg
     3241 tttttggggc cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc
     3301 ggggcctgcg agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg
     3361 ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc
     3421 ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct
     3481 caaaatggag gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa
     3541 gggcctttcc gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca
     3601 ggcacctcga ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt
     3661 tttatgcgat ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc
     3721 acttgatgta attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca
     3781 agcctcagac agtggttcaa agtttttttc ttccatttca ggtgtcgtga cgtacggcca
     3841 ccatgggtag cggacctaag aaaaagagga aggtggcggc cgctggaggt tctggaagtg
     3901 gatccatggt ggataacaaa ttcaataaag aagtgctggt ggcccggcag gaaatctatt
     3961 ggctgccaaa cctgaattgg gagcagaagt ttgccttcat cagtagcctg actaatgatc
     4021 catctcagag cgccaatctg ctggccgaag ccaaaaaact gaacggtgcc cagcccccaa
     4081 aaggaggagg tggaagcgga ggcggcggaa gtgcggccgc tggatccgga cgggctgacg
     4141 cattggacga ttttgatctg gatatgctgg gaagtgacgc cctcgatgat tttgaccttg
     4201 acatgcttgg ttcggatgcc cttgatgact ttgacctcga catgctcggc agtgacgccc
     4261 ttgatgattt cgacctggac atgctgatta acactagtgg cggctctggc ggcggctccg
     4321 gaggctctag ccagtacctg cccgacaccg acgacaggca ccggatcgag gaaaagcgga
     4381 agcggaccta cgagacattc aagagcatca tgaagaagtc ccccttcagc ggccccaccg
     4441 accctagacc tccacctaga agaatcgccg tgcccagcag atccagcgcc agcgtgccaa
     4501 aacctgcccc ccagccttac cccttcacca gcagcctgag caccatcaac tacgacgagt
     4561 tccctaccat ggtgttcccc agcggccaga tctctcaggc ctctgctctg gctccagccc
     4621 ctcctcaggt gctgcctcag gctcctgctc ctgcaccagc tccagccatg gtgtctgcac
     4681 tggctcaggc accagcaccc gtgcctgtgc tggctcctgg acctccacag gctgtggctc
     4741 caccagcccc taaacctaca caggccggcg agggcacact gtctgaagct ctgctgcagc
     4801 tgcagttcga cgacgaggat ctgggagccc tgctgggaaa cagcaccgat cctgccgtgt
     4861 tcaccgacct ggccagcgtg gacaacagcg agttccagca gctgctgaac cagggcatcc
     4921 ctgtggcccc tcacaccacc gagcccatgc tgatggaata ccccgaggcc atcacccggc
     4981 tcgtgacagg cgctcagagg cctcctgatc cagctcctgc ccctctggga gcaccaggcc
     5041 tgcctaatgg actgctgtct ggcgacgagg acttcagctc tatcgccgat atggatttct
     5101 cagccttgct gggctctggc agcggcagcc gggattccag ggaagggatg tttttgccga
     5161 agcctgaggc cggctccgct attagtgacg tgtttgaggg ccgcgaggtg tgccagccaa
     5221 aacgaatccg gccatttcat cctccaggaa gtccatgggc caaccgccca ctccccgcca
     5281 gcctcgcacc aacaccaacc ggtccagtac atgagccagt cgggtcactg accccggcac
     5341 cagtccctca gccactggac ccagcgcccg cagtgactcc cgaggccagt cacctgttgg
     5401 aggaccccga tgaagagacg agccaggctg tcaaagccct tcgggagatg gccgatactg
     5461 tgattcccca gaaggaagag gctgcaatct gtggccaaat ggacctttcc catccgcccc
     5521 caaggggcca tctggatgag ctgacaacca cacttgagtc catgaccgag gatctgaacc
     5581 tggactcacc cctgaccccg gaattgaacg agattctgga taccttcctg aacgacgagt
     5641 gcctcttgca tgccatgcat atcagcacag gactgtccat cttcgacaca tctctgtttg
     5701 gttctggaag tggatccagc cccaagaaga agagaaaggt ggaggccagc taaagcgaag
     5761 tcaatcccag aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt
     5821 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     5881 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     5941 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     6001 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     6061 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     6121 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     6181 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     6241 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     6301 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     6361 tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gagacctaga aaaacatgga
     6421 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa
     6481 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac
     6541 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     6601 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     6661 cctgattggc agaactacac accagggcca gggatcagat atccactgac ctttggatgg
     6721 tgctacaagc tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac
     6781 acccgcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta
     6841 gagtggaggt ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac
     6901 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     6961 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     7021 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     7081 tctagcaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     7141 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     7201 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     7261 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     7321 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
     7381 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg gggggggggg tggggtgggg
     7441 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     7501 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     7561 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     7621 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     7681 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     7741 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     7801 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     7861 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     7921 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     7981 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     8041 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     8101 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     8161 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     8221 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     8281 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     8341 gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat
     8401 cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt
     8461 tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg
     8521 gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct
     8581 gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt
     8641 gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga
     8701 cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct
     8761 gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct
     8821 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     8881 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     8941 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     9001 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     9061 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
     9121 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     9181 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     9241 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     9301 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     9361 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     9421 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     9481 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     9541 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     9601 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     9661 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     9721 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     9781 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     9841 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     9901 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     9961 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
    10021 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
    10081 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
    10141 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
    10201 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
    10261 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
    10321 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
    10381 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
    10441 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
    10501 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
    10561 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
    10621 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
    10681 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
    10741 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
    10801 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
    10861 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
    10921 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
    10981 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
    11041 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
    11101 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
    11161 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    11221 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg
    11281 agatctcccg atcccc
//
