LOCUS       sequence-462707-        7897 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7897)
  AUTHORS   Karunakaran R, Mauchline TH, Hosie AH, Poole PS
  TITLE     A family of promoter probe vectors incorporating autofluorescent and
            chromogenic reporter proteins for studying gene expression in 
            Gram-negative bacteria.
  JOURNAL   Microbiology. 2005 Oct . 151(Pt 10):3249-56.
  PUBMED    16207908
REFERENCE   2  (bases 1 to 7897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Microbiology. 2005 Oct . 151(Pt 10):3249-56."
COMMENT     Sequence Label: pRU1103
FEATURES             Location/Qualifiers
     source          1..7897
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     3518..3538
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      3598..3684
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(4374..5036)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      5037..5808
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             complement(7090..7623)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(7812..7817,7818..7834,7835..7840))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 tttactaagc tgatccggtg gatgaccttt tgaatgacct ttaatagatt atattactaa
       61 ttaattgggg accctagagg tccccttttt tattttaaaa attttttcac aaaacggttt
      121 acaagcataa agcttgctca atcaatcacc ggatctagat atcgattaat taagtcgaca
      181 tctagagttt aaactttaat taagcccggg ctgcagcccg ggggatccac taggttaacg
      241 catgcggccc ggtaccaggc ctagatagat agagagagag agagactagt ggaggaagaa
      301 aaaatgacca tgattacgga ttcactggcc gtcgtggccc gcaccgatcg cccttcccaa
      361 cagttgcgca gcctgaatgg cgaatggcgc tttgcctggt ttccggcacc agaagcggtg
      421 ccggaaagct ggctggagtg cgatcttcct gaggccgata ctgtcgtcgt cccctcaaac
      481 tggcagatgc acggttacga tgcgcccatc tacaccaacg tgacctatcc cattacggtc
      541 aatccgccgt ttgttcccac ggagaatccg acgggttgtt actcgctcac atttaatgtt
      601 gatgaaagct ggctacagga aggccagacg cgaattattt ttgatggcgt taactcggcg
      661 tttcatctgt ggtgcaacgg gcgctgggtc ggttacggcc aggacagtcg tttgccgtct
      721 gaatttgacc tgagcgcatt tttacgcgcc ggagaaaacc gcctcgcggt gatggtgctg
      781 cgctggagtg acggcagtta tctggaagat caggatatgt ggcggatgag cggcattttc
      841 cgtgacgtct cgttgctgca taaaccgact acacaaatca gcgatttcca tgttgccact
      901 cgctttaatg atgatttcag ccgcgctgta ctggaggctg aagttcagat gtgcggcgag
      961 ttgcgtgact acctacgggt aacagtttct ttatggcagg gtgaaacgca ggtcgccagc
     1021 ggcaccgcgc ctttcggcgg tgaaattatc gatgagcgtg gtggttatgc cgatcgcgtc
     1081 acactacgtc tgaacgtcga aaacccgaaa ctgtggagcg ccgaaatccc gaatctctat
     1141 cgtgcggtgg ttgaactgca caccgccgac ggcacgctga ttgaagcaga agcctgcgat
     1201 gtcggtttcc gcgaggtgcg gattgaaaat ggtctgctgc tgctgaacgg caagccgttg
     1261 ctgattcgag gcgttaaccg tcacgagcat catcctctgc atggtcaggt catggatgag
     1321 cagacgatgg tgcaggatat cctgctgatg aagcagaaca actttaacgc cgtgcgctgt
     1381 tcgcattatc cgaaccatcc gctgtggtac acgctgtgcg accgctacgg cctgtatgtg
     1441 gtggatgaag ccaatattga aacccacggc atggtgccaa tgaatcgtct gaccgatgat
     1501 ccgcgctggc taccggcgat gagcgaacgc gtaacgcgaa tggtgcagcg cgatcgtaat
     1561 cacccgagtg tgatcatctg gtcgctgggg aatgaatcag gccacggcgc taatcacgac
     1621 gcgctgtatc gctggatcaa atctgtcgat ccttcccgcc cggtgcagta tgaaggcggc
     1681 ggagccgaca ccacggccac cgatattatt tgcccgatgt acgcgcgcgt ggatgaagac
     1741 cagcccttcc cggctgtgcc gaaatggtcc atcaaaaaat ggctttcgct acctggagag
     1801 acgcgcccgc tgatcctttg cgaatacgcc cacgcgatgg gtaacagtct tggcggtttc
     1861 gctaaatact ggcaggcgtt tcgtcagtat ccccgtttac agggcggctt cgtctgggac
     1921 tgggtggatc agtcgctgat taaatatgat gaaaatggca acccgtggtc ggcttacggc
     1981 ggtgattttg gcgatacgcc gaacgatcgc cagttctgta tgaacggtct ggtctttgcc
     2041 gaccgcacgc cgcatccagc gctgacggaa gcaaaacacc agcagcagtt tttccagttc
     2101 cgtttatccg ggcaaaccat cgaagtgacc agcgaatacc tgttccgtca tagcgataac
     2161 gagctcctgc actggatggt ggcgctggat ggtaagccgc tggcaagcgg tgaagtgcct
     2221 ctggatgtcg ctccacaagg taaacagttg attgaactgc ctgaactacc gcagccggag
     2281 agcgccgggc aactctggct cacagtacgc gtagtgcaac cgaacgcgac cgcatggtca
     2341 gaagccgggc acatcagcgc ctggcagcag tggcgtctgg cggaaaacct cagtgtgacg
     2401 ctccccgccg cgtcccacgc catcccgcat ctgaccacca gcgaaatgga tttttgcatc
     2461 gagctgggta ataagcgttg gcaatttaac cgccagtcag gctttctttc acagatgtgg
     2521 attggcgata aaaaacaact gctgacgccg ctgcgcgatc agttcacccg tgcaccgctg
     2581 gataacgaca ttggcgtaag tgaagcgacc cgcattgacc ctaacgcctg ggtcgaacgc
     2641 tggaaggcgg cgggccatta ccaggccgaa gcagcgttgt tgcagtgcac ggcagataca
     2701 cttgctgatg cggtgctgat tacgaccgct cacgcgtggc agcatcaggg gaaaacctta
     2761 tttatcagcc ggaaaaccta ccggattgat ggtagtggtc aaatggcgat taccgttgat
     2821 gttgaagtgg cgagcgatac accgcatccg gcgcggattg gcctgaactg ccagctggcg
     2881 caggtagcag agcgggtaaa ctggctcgga ttagggccgc aagaaaacta tcccgaccgc
     2941 cttactgccg cctgttttga ccgctgggat ctgccattgt cagacatgta taccccgtac
     3001 gtcttcccga gcgaaaacgg tctgcgctgc gggacgcgcg aattgaatta tggcccacac
     3061 cagtggcgcg gcgacttcca gttcaacatc agccgctaca gtcaacagca actgatggaa
     3121 accagccatc gccatctgct gcacgcggaa gaaggcacat ggctgaatat cgacggtttc
     3181 catatgggga ttggtggcga cgactcctgg agcccgtcag tatcggcgga attccagctg
     3241 agcgccggtc gctaccatta ccagttggtc tggtgtaaaa ataactcgag tacaactata
     3301 actcacacaa tgtatacatc acggcagaca aacaaaagaa tggaatcaaa gctaacttca
     3361 aaattcgcca caacattgaa gatggatccg ttcaactagc agaccattat caacaaaata
     3421 ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg
     3481 ccctttcgaa agatcccaac gaaaagcgtg accacatggt ccttcttgag tttgtaactg
     3541 ctgctgggat tacacatggc atggatgagc tctacaaata atgaattccc caggcatcaa
     3601 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     3661 aacgctctcc tgagtaggac aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg
     3721 cccggagggt ggcgggcagg acgcccgcca taaactgcca ggaattccaa ctgagcgccg
     3781 gtcgctacca ttaccaactt gtctggtgtc aaaaataata ggattttgtt aaaattcgcg
     3841 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgtactgcg atgagtggca
     3901 gggcggggcg taattttttt aaggcagtta ttggtgccct taaacgcctg gttgctacgc
     3961 ctgaataagt gataataagc ggatgaatgg cagaaattcg aaagcaaatt cgacccggtc
     4021 gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct attgctggtt taccggttta
     4081 ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg cctgaggcca gtttgctcag
     4141 gctctccccg tggaggtaat aattgacgat atgatcattt attctgcctc ccagagcctg
     4201 ataaaaacgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc
     4261 ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgaggcggc
     4321 tacagccgat agtctggaac agcgcactta cgggttgctg cgcaacccaa gtgctaccgg
     4381 cgcggcagcg tgacccgtgt cggcggctcc aacggctcgc catcgtccag aaaacacggc
     4441 tcatcgggca tcggcaggcg ctgctgcccg cgccgttccc attcctccgt ttcggtcaag
     4501 gctggcaggt ctggttccat gcccggaatg ccgggctggc tgggcggctc ctcgccgggg
     4561 ccggtcggta gttgctgctc gcccggatac agggtcggga tgcggcgcag gtcgccatgc
     4621 cccaacagcg attcgtcctg gtcgtcgtga tcaaccacca cggcggcact gaacaccgac
     4681 aggcgcaact ggtcgcgggg ctggccccac gccacgcggt cattgaccac gtaggccgac
     4741 acggtgccgg ggccgttgag cttcacgacg gagatccagc gctcggccac caagtccttg
     4801 actgcgtatt ggaccgtccg caaagaacgt ccgatgagct tggaaagtgt cttctggctg
     4861 accaccacgg cgttctggtg gcccatctgc gccacgaggt gatgcagcag cattgccgcc
     4921 gtgggtttcc tcgcaataag cccggcccac gcctcatgcg ctttgcgttc cgtttgcacc
     4981 cagtgaccgg gcttgttctt ggcttgaatg ccgatttctc tggactgcgt ggccatgctt
     5041 atctccatgc ggtaggggtg ccgcacggtt gcggcaccat gcgcaatcag ctgcaacttt
     5101 tcggcagcgc gacaacaatt atgcgttgcg taaaagtggc agtcaattac agattttctt
     5161 taacctacgc aatgagctat tgcggggggt gccgcaatga gctgttgcgt accccccttt
     5221 tttaagttgt tgatttttaa gtctttcgca tttcgcccta tatctagttc tttggtgccc
     5281 aaagaagggc acccctgcgg ggttccccca cgccttcggc gcggctcccc ctccggcaaa
     5341 aagtggcccc tccggggctt gttgatcgac tgcgcggcct tcggccttgc ccaaggtggc
     5401 gctgccccct tggaaccccc gcactcgccg ccgtgaggct cggggggcag gcgggcgggc
     5461 ttcgcccttc gactgccccc actcgcatag gcttgggtcg ttccaggcgc gtcaaggcca
     5521 agccgctgcg cggtcgctgc gcgagccttg acccgccttc cacttggtgt ccaaccggca
     5581 agcgaagcgc gcaggccgca ggccggaggc ttttccccag agaaaattaa aaaaattgat
     5641 ggggcaaggc cgcaggccgc gcagttggag ccggtgggta tgtggtcgaa ggctgggtag
     5701 ccggtgggca atccctgtgg tcaagctcgt gggcaggcgc agcctgtcca tcagcttgtc
     5761 cagcagggtt gtccacgggc cgagcgaagc gagccagccg gtggccgctc gcggccatcg
     5821 tccacatatc cacgggctgg caagggagcg cagcgaccgc gcagggcgaa gcccggagag
     5881 caagcccgta gggcgccgca gccgccgtag gcggtcacga ctttgcgaag caaagtctag
     5941 tgagtatact caagcattga gtggcccgcc ggaggcaccg ccttgcgctg cccccgtcga
     6001 gccggttgga caccaaaagg gaggggcagg catggcggca tacgcgatca tgcgatgcaa
     6061 gaagctggcg aaaatgggca acgtggcggc cagtctcaag cacgcctacc gcgagcgcga
     6121 gacgcccaac gctgacgcca gcaggacgcc agagaacgag cactgggcgg ccagcagcac
     6181 cgatgaagcg atgggccgac tgcgcgagtt gctgccagag aagcggcgca aggacgctgt
     6241 gttggcggtc gagtacgtca tgacggccag cccggaatgg tggaagtcgg ccagccaaga
     6301 acagcaggcg gcgttcttcg agaaggcgca caagtggctg gcggacaagt acggggcgga
     6361 tcgcatcgtg acggccagca tccaccgtga cgaaaccagc ccgcacatga ccgcgttcgt
     6421 ggtgccgctg acgcaggacg gcaggctgtc ggccaaggag ttcatcggca acaaagcgca
     6481 gatgacccgc gaccagacca cgtttgcggc cgctgtggcc gatctagggc tgcaacgggg
     6541 catcgagggc agcaaggcac gtcacacgcg cattcaggcg ttctacgagg ccctggagcg
     6601 gccaccagtg ggccacgtca ccatcagccc gcaagcggtc gagccacgcg cctatgcacc
     6661 gcagggattg gccgaaaagc tgggaatctc aaagcgcgtt gagacgccgg aagccgtggc
     6721 cgaccggctg acaaaagcgg ttcggcaggg gtatgagcct gccctacagg ccgccgcagg
     6781 agcgcgtgag atgcgcaaga aggccgatca agcccaagag acggcccgag accttcggga
     6841 gcgcctgaag cccgttctgg acgccctggg gccgttgaat cgggatatgc aggccaaggc
     6901 cgccgcgatc atcaaggccg tgggcgaaaa gctgctgacg gaacagcggg aagtccagcg
     6961 ccagaaacag gcccagcgcc agcaggaacg cgggcgcgca catttccccg aaaagtgcca
     7021 cctgcggcgt tgtgacaatt taccgaacaa ctccgcggcc gggaagccga tctcggcttg
     7081 aacgaattgt taggtggcgg tacttgggtc gatatcaaag tgcatcactt cttcccgtat
     7141 gcccaacttt gtatagagag ccactgcggg atcgtcaccg taatctgctt gcacgtagat
     7201 cacataagca ccaagcgcgt tggcctcatg cttgaggaga ttgatgagcg cggtggcaat
     7261 gccctgcctc cggtgctctc cggagactgc gagatcatag atatagatct cactacgcgg
     7321 ctgctcaaac ttgggcagaa cgtaagccgc gagagcgcca acaaccgctt cttggtcgaa
     7381 ggcagcaagc gcgatgaatg tcttactacg gagcaagttc ccgaggtaat cggagtccgg
     7441 ctgatgttgg gagtaggtgg ctacgtctcc gaactcacga ccgaaaagat caagagcagc
     7501 ccgcatggat ttgacttggt cagggccgag cctacatgtg cgaatgatgc ccatacttga
     7561 gccacctaac tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctgcgtaa
     7621 catcgttgct gctccataac atcaaacatc gacccacggc gtaacgcgct tgctgcttgg
     7681 atgcccgagg catagactgt acaaaaaaac agtcataaca agccatgaaa accgccactg
     7741 cgccgttacc accgctgcgt tcggtcaagg ttctggacca gttgcgtgag cgcatacgct
     7801 acttgcatta cagtttacga accgaacagg cttatgtcaa ctgggttcgt gccttcatcc
     7861 gtttccacgg tgtgcgtcca tgggcaaatg ggatggc
//
