LOCUS       sequence-462750-        9379 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression plasmid for the ABE8e base editor with superNLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9379)
  AUTHORS   Geilenkeuser J, Armbrust N, Steinmassl E, Du SW, Schmidt S, Binder 
            EMH, Li Y, Warsing NW, Wendel SV, von der Linde F, Schiele EM, Niu 
            X, Stroppel L, Berezin O, Santl TH, Orschmann T, Nelson K, Gruber C,
            Palczewska G, Menezes CR, Risaliti E, Engfer ZJ, Koleci N, Schmidts 
            A, Geerlof A, Palczewski K, Westmeyer GG, Truong DJ
  TITLE     Engineered nucleocytosolic vehicles for loading of programmable 
            editors.
  JOURNAL   Cell. 2025 May 15;188(10):2637-2655.e31. doi: 
            10.1016/j.cell.2025.03.015. Epub 2025 Apr 9.
  PUBMED    40209705
REFERENCE   2  (bases 1 to 9379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2025.03.015"; journalName: "Cell"; date: 
            "2025-05-15- 15"; volume: "188"; issue: "10"; pages: "2637-2655.e31"
COMMENT     Sequence Label: pCMV_ABE8e–superNLS
FEATURES             Location/Qualifiers
     source          1..9379
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      489..1077
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     978..997
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1231..1248
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1365..1386
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1401..1406,1407..1424,1425..1431)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1439..1455
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1444..1466
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1463..1479
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1463..1479
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        1503..1882
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1883..2086
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2037..2057
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2083..2107
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          2198..2770
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     2825..2844
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2825..2843
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2890..2910
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(3505..3528,3529..3531,3532..7605)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             3505..7605
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7618..7638
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7654..7680
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             7693..7719
                     /codon_start=1
                     /product="nuclear localization signal (Makkerh et al., 
                     1996)"
                     /label=NLS
                     /translation="PAAKKKKLD"
     CDS             7717..7740
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(7761..7777)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7791..7808)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7797..8021
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8093..8110)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8093..8109)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8102..8124)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(8250..8705)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8387..8408)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8599..8618
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8732..8836
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8837..8905,8906..9379,1..318)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9055..9074)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
       61 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
      121 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
      181 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
      241 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
      301 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
      361 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
      421 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
      481 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
      541 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
      601 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
      661 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
      721 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
      781 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      841 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
      901 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      961 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     1021 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     1081 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     1141 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     1201 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     1261 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     1321 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     1381 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     1441 gtgagcggat aacaatttca cacaggaaac agctatgacc atgaggcgcg ccccgccatg
     1501 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
     1561 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     1621 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     1681 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     1741 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     1801 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     1861 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
     1921 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     1981 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     2041 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg
     2101 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg
     2161 atccagcctc cggtcgaccg atcctgagaa cttcagggtg agtttgggga cccttgattg
     2221 ttctttcttt ttcgctattg taaaattcat gttatatgga gggggcaaag ttttcagggt
     2281 gttgtttaga atgggaagat gtcccttgta tcaccatgga ccctcatgat aattttgttt
     2341 ctttcacttt ctactctgtt gacaaccatt gtctcctctt attttctttt cattttctgt
     2401 aactttttcg ttaaacttta gcttgcattt gtaacgaatt tttaaattca cttttgttta
     2461 tttgtcagat tgtaagtact ttctctaatc actttttttt caaggcaatc agggtatatt
     2521 atattgtact tcagcacagt tttagagaac aattgttata attaaatgat aaggtagaat
     2581 atttctgcat ataaattctg gctggcgtgg aaatattctt attggtagaa acaactacac
     2641 cctggtcatc atcctgcctt tctctttatg gttacaatga tatacactgt ttgagatgag
     2701 gataaaatac tctgagtcca aaccgggccc ctctgctaac catgttcatg ccttcttctc
     2761 tttcctacag ctcctgggca acgtgctggt tgttgtgctg tctcatcatt ttggcattaa
     2821 ttaataatac gactcactat aggggccgcc accatgaaac ggacagccga cggaagcgag
     2881 ttcgagtcac caaagaagaa gcggaaagtc tctgaggtgg agttttccca cgagtactgg
     2941 atgagacatg ccctgaccct ggccaagagg gcacgggatg agagggaggt gcctgtggga
     3001 gccgtgctgg tgctgaacaa tagagtgatc ggcgagggct ggaacagagc catcggcctg
     3061 cacgacccaa cagcccatgc cgaaattatg gccctgagac agggcggcct ggtcatgcag
     3121 aactacagac tgattgacgc caccctgtac gtgacattcg agccttgcgt gatgtgcgcc
     3181 ggcgccatga tccactctag gatcggccgc gtggtgtttg gcgtgaggaa ctcaaaaaga
     3241 ggcgccgcag gctccctgat gaacgtgctg aactaccccg gcatgaatca ccgcgtcgaa
     3301 attaccgagg gaatcctggc agatgaatgt gccgccctgc tgtgcgattt ctatcggatg
     3361 cctagacagg tgttcaatgc tcagaagaag gcccagagct ccatcaactc cggaggatct
     3421 agcggaggct cctctggctc tgagacacct ggcacaagcg agagcgcaac acctgaaagc
     3481 agcgggggca gcagcggggg gtcagacaag aagtacagca tcggcctggc catcggcacc
     3541 aactctgtgg gctgggccgt gatcaccgac gagtacaagg tgcccagcaa gaaattcaag
     3601 gtgctgggca acaccgaccg gcacagcatc aagaagaacc tgatcggagc cctgctgttc
     3661 gacagcggcg aaacagccga ggccacccgg ctgaagagaa ccgccagaag aagatacacc
     3721 agacggaaga accggatctg ctatctgcaa gagatcttca gcaacgagat ggccaaggtg
     3781 gacgacagct tcttccacag actggaagag tccttcctgg tggaagagga taagaagcac
     3841 gagcggcacc ccatcttcgg caacatcgtg gacgaggtgg cctaccacga gaagtacccc
     3901 accatctacc acctgagaaa gaaactggtg gacagcaccg acaaggccga cctgcggctg
     3961 atctatctgg ccctggccca catgatcaag ttccggggcc acttcctgat cgagggcgac
     4021 ctgaaccccg acaacagcga cgtggacaag ctgttcatcc agctggtgca gacctacaac
     4081 cagctgttcg aggaaaaccc catcaacgcc agcggcgtgg acgccaaggc catcctgtct
     4141 gccagactga gcaagagcag acggctggaa aatctgatcg cccagctgcc cggcgagaag
     4201 aagaatggcc tgttcggcaa cctgattgcc ctgagcctgg gcctgacccc caacttcaag
     4261 agcaacttcg acctggccga ggatgccaaa ctgcagctga gcaaggacac ctacgacgac
     4321 gacctggaca acctgctggc ccagatcggc gaccagtacg ccgacctgtt tctggccgcc
     4381 aagaacctgt ccgacgccat cctgctgagc gacatcctga gagtgaacac cgagatcacc
     4441 aaggcccccc tgagcgcctc tatgatcaag agatacgacg agcaccacca ggacctgacc
     4501 ctgctgaaag ctctcgtgcg gcagcagctg cctgagaagt acaaagagat tttcttcgac
     4561 cagagcaaga acggctacgc cggctacatt gacggcggag ccagccagga agagttctac
     4621 aagttcatca agcccatcct ggaaaagatg gacggcaccg aggaactgct cgtgaagctg
     4681 aacagagagg acctgctgcg gaagcagcgg accttcgaca acggcagcat cccccaccag
     4741 atccacctgg gagagctgca cgccattctg cggcggcagg aagattttta cccattcctg
     4801 aaggacaacc gggaaaagat cgagaagatc ctgaccttcc gcatccccta ctacgtgggc
     4861 cctctggcca ggggaaacag cagattcgcc tggatgacca gaaagagcga ggaaaccatc
     4921 accccctgga acttcgagga agtggtggac aagggcgctt ccgcccagag cttcatcgag
     4981 cggatgacca acttcgataa gaacctgccc aacgagaagg tgctgcccaa gcacagcctg
     5041 ctgtacgagt acttcaccgt gtataacgag ctgaccaaag tgaaatacgt gaccgaggga
     5101 atgagaaagc ccgccttcct gagcggcgag cagaaaaagg ccatcgtgga cctgctgttc
     5161 aagaccaacc ggaaagtgac cgtgaagcag ctgaaagagg actacttcaa gaaaatcgag
     5221 tgcttcgact ccgtggaaat ctccggcgtg gaagatcggt tcaacgcctc cctgggcaca
     5281 taccacgatc tgctgaaaat tatcaaggac aaggacttcc tggacaatga ggaaaacgag
     5341 gacattctgg aagatatcgt gctgaccctg acactgtttg aggacagaga gatgatcgag
     5401 gaacggctga aaacctatgc ccacctgttc gacgacaaag tgatgaagca gctgaagcgg
     5461 cggagataca ccggctgggg caggctgagc cggaagctga tcaacggcat ccgggacaag
     5521 cagtccggca agacaatcct ggatttcctg aagtccgacg gcttcgccaa cagaaacttc
     5581 atgcagctga tccacgacga cagcctgacc tttaaagagg acatccagaa agcccaggtg
     5641 tccggccagg gcgatagcct gcacgagcac attgccaatc tggccggcag ccccgccatt
     5701 aagaagggca tcctgcagac agtgaaggtg gtggacgagc tcgtgaaagt gatgggccgg
     5761 cacaagcccg agaacatcgt gatcgaaatg gccagagaga accagaccac ccagaaggga
     5821 cagaagaaca gccgcgagag aatgaagcgg atcgaagagg gcatcaaaga gctgggcagc
     5881 cagatcctga aagaacaccc cgtggaaaac acccagctgc agaacgagaa gctgtacctg
     5941 tactacctgc agaatgggcg ggatatgtac gtggaccagg aactggacat caaccggctg
     6001 tccgactacg atgtggacca tatcgtgcct cagagctttc tgaaggacga ctccatcgac
     6061 aacaaggtgc tgaccagaag cgacaagaac cggggcaaga gcgacaacgt gccctccgaa
     6121 gaggtcgtga agaagatgaa gaactactgg cggcagctgc tgaacgccaa gctgattacc
     6181 cagagaaagt tcgacaatct gaccaaggcc gagagaggcg gcctgagcga actggataag
     6241 gccggcttca tcaagagaca gctggtggaa acccggcaga tcacaaagca cgtggcacag
     6301 atcctggact cccggatgaa cactaagtac gacgagaatg acaagctgat ccgggaagtg
     6361 aaagtgatca ccctgaagtc caagctggtg tccgatttcc ggaaggattt ccagttttac
     6421 aaagtgcgcg agatcaacaa ctaccaccac gcccacgacg cctacctgaa cgccgtcgtg
     6481 ggaaccgccc tgatcaaaaa gtaccctaag ctggaaagcg agttcgtgta cggcgactac
     6541 aaggtgtacg acgtgcggaa gatgatcgcc aagagcgagc aggaaatcgg caaggctacc
     6601 gccaagtact tcttctacag caacatcatg aactttttca agaccgagat taccctggcc
     6661 aacggcgaga tccggaagcg gcctctgatc gagacaaacg gcgaaaccgg ggagatcgtg
     6721 tgggataagg gccgggattt tgccaccgtg cggaaagtgc tgagcatgcc ccaagtgaat
     6781 atcgtgaaaa agaccgaggt gcagacaggc ggcttcagca aagagtctat cctgcccaag
     6841 aggaacagcg ataagctgat cgccagaaag aaggactggg accctaagaa gtacggcggc
     6901 ttcgacagcc ccaccgtggc ctattctgtg ctggtggtgg ccaaagtgga aaagggcaag
     6961 tccaagaaac tgaagagtgt gaaagagctg ctggggatca ccatcatgga aagaagcagc
     7021 ttcgagaaga atcccatcga ctttctggaa gccaagggct acaaagaagt gaaaaaggac
     7081 ctgatcatca agctgcctaa gtactccctg ttcgagctgg aaaacggccg gaagagaatg
     7141 ctggcctctg ccggcgaact gcagaaggga aacgaactgg ccctgccctc caaatatgtg
     7201 aacttcctgt acctggccag ccactatgag aagctgaagg gctcccccga ggataatgag
     7261 cagaaacagc tgtttgtgga acagcacaag cactacctgg acgagatcat cgagcagatc
     7321 agcgagttct ccaagagagt gatcctggcc gacgctaatc tggacaaagt gctgtccgcc
     7381 tacaacaagc accgggataa gcccatcaga gagcaggccg agaatatcat ccacctgttt
     7441 accctgacca atctgggagc ccctgccgcc ttcaagtact ttgacaccac catcgaccgg
     7501 aagaggtaca ccagcaccaa agaggtgctg gacgccaccc tgatccacca gagcatcacc
     7561 ggcctgtacg agacacggat cgacctgtct cagctgggag gcgacggatc cacaccacct
     7621 aagaagaaac ggaaggtcga ggacggcgag ggccctgctg ctaagagagt gaaactggac
     7681 tccggagctg ctccagccgc caagaagaag aagctcgact acaaggacga cgacgataag
     7741 tgaacgcgta aatgattgca gatccactag ttctagagct cgctgatcag cctcgactgt
     7801 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     7861 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     7921 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     7981 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     8041 cagctggggc tcgagatcca ctagttctag cctcgaggct agagcggccg ccactggccg
     8101 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     8161 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     8221 aacagttgcg cagcctgaat ggcgaatgga aattgtaagc gttaatattt tgttaaaatt
     8281 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     8341 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     8401 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     8461 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     8521 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     8581 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     8641 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     8701 cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     8761 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     8821 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     8881 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     8941 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     9001 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     9061 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     9121 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     9181 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     9241 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     9301 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     9361 gcgtgacacc acgatgcct
//
