LOCUS       sequence-462752-       11527 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression plasmid for iPE-C prime editor RNPs with additional Csy4 
            protection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11527)
  AUTHORS   Geilenkeuser J, Armbrust N, Steinmassl E, Du SW, Schmidt S, Binder 
            EMH, Li Y, Warsing NW, Wendel SV, von der Linde F, Schiele EM, Niu 
            X, Stroppel L, Berezin O, Santl TH, Orschmann T, Nelson K, Gruber C,
            Palczewska G, Menezes CR, Risaliti E, Engfer ZJ, Koleci N, Schmidts 
            A, Geerlof A, Palczewski K, Westmeyer GG, Truong DJ
  TITLE     Engineered nucleocytosolic vehicles for loading of programmable 
            editors.
  JOURNAL   Cell. 2025 May 15;188(10):2637-2655.e31. doi: 
            10.1016/j.cell.2025.03.015. Epub 2025 Apr 9.
  PUBMED    40209705
REFERENCE   2  (bases 1 to 11527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2025.03.015"; journalName: "Cell"; date: 
            "2025-05-15- 15"; volume: "188"; issue: "10"; pages: "2637-2655.e31"
COMMENT     Sequence Label: pCMV_iPE-C_P2A_Csy4
FEATURES             Location/Qualifiers
     source          1..11527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      489..1077
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     978..997
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1231..1248
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1365..1386
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1401..1406,1407..1424,1425..1431)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1439..1455
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1444..1466
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1463..1479
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1463..1479
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        1503..1882
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1883..2086
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2037..2057
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2083..2107
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          2198..2770
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     2825..2844
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2825..2843
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2848..2857
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2854..2880,2881..2883,2884..5439,5440..5442,
                     5443..6957)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the N863A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6961..6987
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6967..6993
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             7006..7026
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7042..7068
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             7081..7107
                     /codon_start=1
                     /product="nuclear localization signal (Makkerh et al., 
                     1996)"
                     /label=NLS
                     /translation="PAAKKKKLD"
     CDS             9238..9258
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9271..9327
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(9909..9925)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9939..9956)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9945..10169
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(10241..10258)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10241..10257)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10250..10272)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(10398..10853)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10535..10556)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10747..10766
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        10880..10984
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10985..11053,11054..11527,1..318)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11203..11222)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
       61 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
      121 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
      181 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
      241 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
      301 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
      361 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
      421 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
      481 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
      541 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
      601 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
      661 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
      721 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
      781 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      841 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
      901 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      961 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     1021 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     1081 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     1141 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     1201 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     1261 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     1321 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     1381 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     1441 gtgagcggat aacaatttca cacaggaaac agctatgacc atgaggcgcg ccccgccatg
     1501 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
     1561 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     1621 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     1681 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     1741 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     1801 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     1861 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
     1921 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     1981 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     2041 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg
     2101 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg
     2161 atccagcctc cggtcgaccg atcctgagaa cttcagggtg agtttgggga cccttgattg
     2221 ttctttcttt ttcgctattg taaaattcat gttatatgga gggggcaaag ttttcagggt
     2281 gttgtttaga atgggaagat gtcccttgta tcaccatgga ccctcatgat aattttgttt
     2341 ctttcacttt ctactctgtt gacaaccatt gtctcctctt attttctttt cattttctgt
     2401 aactttttcg ttaaacttta gcttgcattt gtaacgaatt tttaaattca cttttgttta
     2461 tttgtcagat tgtaagtact ttctctaatc actttttttt caaggcaatc agggtatatt
     2521 atattgtact tcagcacagt tttagagaac aattgttata attaaatgat aaggtagaat
     2581 atttctgcat ataaattctg gctggcgtgg aaatattctt attggtagaa acaactacac
     2641 cctggtcatc atcctgcctt tctctttatg gttacaatga tatacactgt ttgagatgag
     2701 gataaaatac tctgagtcca aaccgggccc ctctgctaac catgttcatg ccttcttctc
     2761 tttcctacag ctcctgggca acgtgctggt tgttgtgctg tctcatcatt ttggcattaa
     2821 ttaataatac gactcactat aggggccgcc accatggaca agaagtacag catcggcctg
     2881 gacatcggca ccaactctgt gggctgggcc gtgatcaccg acgagtacaa ggtgcccagc
     2941 aagaaattca aggtgctggg caacaccgac cggcacagca tcaagaagaa cctgatcgga
     3001 gccctgctgt tcgacagcgg cgaaacagcc gaggccaccc ggctgaagag aaccgccaga
     3061 agaagataca ccagacggaa gaaccggatc tgctatctgc aagagatctt cagcaacgag
     3121 atggccaagg tggacgacag cttcttccac agactggaag agtccttcct ggtggaagag
     3181 gataagaagc acgagcggca ccccatcttc ggcaacatcg tggacgaggt ggcctaccac
     3241 gagaagtacc ccaccatcta ccacctgaga aagaaactgg tggacagcac cgacaaggcc
     3301 gacctgcggc tgatctatct ggccctggcc cacatgatca agttccgggg ccacttcctg
     3361 atcgagggcg acctgaaccc cgacaacagc gacgtggaca agctgttcat ccagctggtg
     3421 cagacctaca accagctgtt cgaggaaaac cccatcaacg ccagcggcgt ggacgccaag
     3481 gccatcctgt ctgccagact gagcaagagc agacggctgg aaaatctgat cgcccagctg
     3541 cccggcgaga agaagaatgg cctgttcggc aacctgattg ccctgagcct gggcctgacc
     3601 cccaacttca agagcaactt cgacctggcc gaggatgcca aactgcagct gagcaaggac
     3661 acctacgacg acgacctgga caacctgctg gcccagatcg gcgaccagta cgccgacctg
     3721 tttctggccg ccaagaacct gtccgacgcc atcctgctga gcgacatcct gagagtgaac
     3781 accgagatca ccaaggcccc cctgagcgcc tctatgatca agagatacga cgagcaccac
     3841 caggacctga ccctgctgaa agctctcgtg cggcagcagc tgcctgagaa gtacaaagag
     3901 attttcttcg accagagcaa gaacggctac gccggctaca ttgacggcgg agccagccag
     3961 gaagagttct acaagttcat caagcccatc ctggaaaaga tggacggcac cgaggaactg
     4021 ctcgtgaagc tgaacagaga ggacctgctg cggaagcagc ggaccttcga caacggcagc
     4081 atcccccacc agatccacct gggagagctg cacgccattc tgcggcggca ggaagatttt
     4141 tacccattcc tgaaggacaa ccgggaaaag atcgagaaga tcctgacctt ccgcatcccc
     4201 tactacgtgg gccctctggc caggggaaac agcagattcg cctggatgac cagaaagagc
     4261 gaggaaacca tcaccccctg gaacttcgag gaagtggtgg acaagggcgc ttccgcccag
     4321 agcttcatcg agcggatgac caacttcgat aagaacctgc ccaacgagaa ggtgctgccc
     4381 aagcacagcc tgctgtacga gtacttcacc gtgtataacg agctgaccaa agtgaaatac
     4441 gtgaccgagg gaatgagaaa gcccgccttc ctgagcggcg agcagaaaaa ggccatcgtg
     4501 gacctgctgt tcaagaccaa ccggaaagtg accgtgaagc agctgaaaga ggactacttc
     4561 aagaaaatcg agtgcttcga ctccgtggaa atctccggcg tggaagatcg gttcaacgcc
     4621 tccctgggca cataccacga tctgctgaaa attatcaagg acaaggactt cctggacaat
     4681 gaggaaaacg aggacattct ggaagatatc gtgctgaccc tgacactgtt tgaggacaga
     4741 gagatgatcg aggaacggct gaaaacctat gcccacctgt tcgacgacaa agtgatgaag
     4801 cagctgaagc ggcggagata caccggctgg ggcaggctga gccggaagct gatcaacggc
     4861 atccgggaca agcagtccgg caagacaatc ctggatttcc tgaagtccga cggcttcgcc
     4921 aacagaaact tcatgcagct gatccacgac gacagcctga cctttaaaga ggacatccag
     4981 aaagcccagg tgtccggcca gggcgatagc ctgcacgagc acattgccaa tctggccggc
     5041 agccccgcca ttaagaaggg catcctgcag acagtgaagg tggtggacga gctcgtgaaa
     5101 gtgatgggcc ggcacaagcc cgagaacatc gtgatcgaaa tggccagaga gaaccagacc
     5161 acccagaagg gacagaagaa cagccgcgag agaatgaagc ggatcgaaga gggcatcaaa
     5221 gagctgggca gccagatcct gaaagaacac cccgtggaaa acacccagct gcagaacgag
     5281 aagctgtacc tgtactacct gcagaatggg cgggatatgt acgtggacca ggaactggac
     5341 atcaaccggc tgtccgacta cgatgtggac catatcgtgc ctcagagctt tctgaaggac
     5401 gactccatcg acaacaaggt gctgaccaga agcgacaagg cccggggcaa gagcgacaac
     5461 gtgccctccg aagaggtcgt gaagaagatg aagaactact ggcggcagct gctgaacgcc
     5521 aagctgatta cccagagaaa gttcgacaat ctgaccaagg ccgagagagg cggcctgagc
     5581 gaactggata aggccggctt catcaagaga cagctggtgg aaacccggca gatcacaaag
     5641 cacgtggcac agatcctgga ctcccggatg aacactaagt acgacgagaa tgacaagctg
     5701 atccgggaag tgaaagtgat caccctgaag tccaagctgg tgtccgattt ccggaaggat
     5761 ttccagtttt acaaagtgcg cgagatcaac aactaccacc acgcccacga cgcctacctg
     5821 aacgccgtcg tgggaaccgc cctgatcaaa aagtacccta agctggaaag cgagttcgtg
     5881 tacggcgact acaaggtgta cgacgtgcgg aagatgatcg ccaagagcga gcaggaaatc
     5941 ggcaaggcta ccgccaagta cttcttctac agcaacatca tgaacttttt caagaccgag
     6001 attaccctgg ccaacggcga gatccggaag cggcctctga tcgagacaaa cggcgaaacc
     6061 ggggagatcg tgtgggataa gggccgggat tttgccaccg tgcggaaagt gctgagcatg
     6121 ccccaagtga atatcgtgaa aaagaccgag gtgcagacag gcggcttcag caaagagtct
     6181 atcctgccca agaggaacag cgataagctg atcgccagaa agaaggactg ggaccctaag
     6241 aagtacggcg gcttcgacag ccccaccgtg gcctattctg tgctggtggt ggccaaagtg
     6301 gaaaagggca agtccaagaa actgaagagt gtgaaagagc tgctggggat caccatcatg
     6361 gaaagaagca gcttcgagaa gaatcccatc gactttctgg aagccaaggg ctacaaagaa
     6421 gtgaaaaagg acctgatcat caagctgcct aagtactccc tgttcgagct ggaaaacggc
     6481 cggaagagaa tgctggcctc tgccggcgaa ctgcagaagg gaaacgaact ggccctgccc
     6541 tccaaatatg tgaacttcct gtacctggcc agccactatg agaagctgaa gggctccccc
     6601 gaggataatg agcagaaaca gctgtttgtg gaacagcaca agcactacct ggacgagatc
     6661 atcgagcaga tcagcgagtt ctccaagaga gtgatcctgg ccgacgctaa tctggacaaa
     6721 gtgctgtccg cctacaacaa gcaccgggat aagcccatca gagagcaggc cgagaatatc
     6781 atccacctgt ttaccctgac caatctggga gcccctgccg ccttcaagta ctttgacacc
     6841 accatcgacc ggaagaggta caccagcacc aaagaggtgc tggacgccac cctgatccac
     6901 cagagcatca ccggcctgta cgagacacgg atcgacctgt ctcagctggg aggcgacgcc
     6961 tatccctatg acgtgcccga ttatgccagc ctgggctccg gatcccccaa gaagaaacgc
     7021 aaggtggaag atggcgaggg cccagccgct aagagagtga aactggattc cggagctgct
     7081 cctgccgcca agaagaagaa actcgacacc ggtggacctg gaagcgagcc tgctacatct
     7141 ggctctgaga cacctggcac ctccgagtct gctacacctg aatctggacc tggcgctagc
     7201 accctgaaca tcgaggacga gtaccggctg cacgagacaa gcaaagaacc cgatgtgtcc
     7261 ctgggcagca cctggcttag tgatttccct caggcctggg ccgagacagg cggaatggga
     7321 cttgctgtta gacaggcccc tctgatcatc cctctgaagg ccacaagcac ccctgtgtcc
     7381 atcaagcagt accccatgag ccaagaggcc cggctgggaa tcaagcccca cattcagaga
     7441 ctgctggacc agggcatcct ggtgccttgt cagagccctt ggaatacccc tctgctgccc
     7501 gtgaagaagc ccggcaccaa cgattacaga cccgtgcagg acctgcggga agtgaacaag
     7561 agagtggaag atattcaccc caccgtgccg aatccttaca acctgctgtc tggcctgcct
     7621 cctagccacc agtggtacac agtgctggac ctgaaggacg ccttcttctg tctgcggctg
     7681 caccctacaa gccagcctct gtttgccttt gagtggcggg accctgagat gggcattagc
     7741 ggacagctga cctggaccag actgccccag ggcttcaaga acagccccac actgttcaac
     7801 gaggccctgc atagggacct cgccgacttc agaatccagc atcctgacct gatcctgctc
     7861 cagtacgtgg acgatctgct gctggccgct acaagcgagc tggattgtca gcagggaaca
     7921 agagccctgc tgcaaaccct gggcaacctg ggctatagag cctctgccaa gaaggcccag
     7981 atttgccaga aacaagtgaa gtatctgggc tacctgctga aagagggcca gcgttggctg
     8041 accgaggcca gaaaagaaac cgtgatgggc cagcctacac ctaagacacc cagacagctg
     8101 agagagttcc tgggcaaagc cggattctgt cggctgttca tccctggctt tgccgagatg
     8161 gctgcccctc tgtacccact gacaaagccc ggaactctgt tcaactgggg cccagatcag
     8221 cagaaggcct accaagagat caagcaggct ctgctgacag cccctgctct gggactgcct
     8281 gatctgacca agcctttcga gctgttcgtg gacgagaagc agggctatgc caaaggcgtg
     8341 ctgacacaga agctcggccc ttggagaagg cctgtggcct acctgagcaa gaaactggac
     8401 cctgtggctg ccggatggcc tccttgtctg agaatggtgg ccgccattgc cgtgctgacc
     8461 aaggatgccg gaaagctgac aatgggacag cctctggtca ttctggcccc tcatgccgtg
     8521 gaagccctcg tgaaacagcc tcctgatcgg tggctgagca acgccaggat gacacactat
     8581 caggcactgc tgctcgacac cgacagagtg cagtttggac ctgtggtggc cctgaatcct
     8641 gccacacttc tgcctctgcc tgaggaaggc ctccagcaca attgcctgga catcctggcc
     8701 gaggctcacg gcacaagacc cgatctgaca gatcagccac tgcctgacgc cgaccacacc
     8761 tggtatacag atggcagctc tctgctgcaa gaaggacaga gaaaagccgg ggctgccgtg
     8821 accaccgaga cagaagtgat ttgggccaaa gctctgcccg ctggcacatc tgctcagaga
     8881 gccgaactga tcgccctgac acaggccctg aaaatggccg agggcaagaa gctgaacgtc
     8941 tacaccgact ccagatacgc cttcgccacc gctcacatcc acggcgaaat ctatcggcgg
     9001 agaggatggc tgaccagcga gggcaaagag attaagaaca aggacgagat tctcgccctg
     9061 ctcaaggccc tgttcctgcc taagcggctg agcatcatcc actgtccagg ccaccagaag
     9121 ggccactctg ctgaagctag aggcaacaga atggccgacc aggctgccag aaaggccgcc
     9181 atcacagaga cacccgatac cagcacactg ctgatcgaga acagcagccc tggtacccct
     9241 aagaagaagc ggaaagtgga cggatccggc gccaccaatt tcagcctgct gaaacaggct
     9301 ggcgacgtgg aagaaaatcc cgggcctatg gaccactacc tggacatcag actgcggccc
     9361 gatcctgagt ttcctccagc tcagctgatg agcgtgctgt tcggcaaact gcaccaggct
     9421 ctggttgccc aaggcggcga tagaatcgga gtgtcattcc ccgacctgga cgagagcaga
     9481 agcagactgg gagagagact gagaatccac gccagcgccg atgatctgag agccctgctt
     9541 gctagacctt ggctggaagg cctgagagat cacctccagt ttggagagcc tgccgtggtg
     9601 cctcatccaa caccatatag acaggtgtcc agagtgcagg ccaagagcaa ccccgaaaga
     9661 ctgcggagaa ggctgatgcg gagacacgac ctgtctgagg aagaggcccg gaagagaatc
     9721 cccgacacag tggctagagc cctggacctg cctttcgtga cactgagaag ccagagcacc
     9781 ggccagcact tccggctgtt tatcagacac ggccctctgc aagtgaccgc cgaagaaggc
     9841 ggctttacct gttacggcct gagcaaaggc ggattcgtgc cctggttttg aacgcgtaaa
     9901 tgattgcaga tccactagtt ctagagctcg ctgatcagcc tcgactgtgc cttctagttg
     9961 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
    10021 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
    10081 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
    10141 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
    10201 gagatccact agttctagcc tcgaggctag agcggccgcc actggccgtc gttttacaac
    10261 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
    10321 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
    10381 gcctgaatgg cgaatggaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
    10441 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
    10501 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
    10561 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
    10621 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
    10681 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
    10741 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
    10801 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg
    10861 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
    10921 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
    10981 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
    11041 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
    11101 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
    11161 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
    11221 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
    11281 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
    11341 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
    11401 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
    11461 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
    11521 gatgcct
//
