LOCUS       sequence-462775-       10277 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A CMV driven human GATA4 followed by a P2A cleavage peptide and an 
            H2A mCherry fusion protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10277)
  AUTHORS   Cantu Gutierrez ME, Hill MC, Largoza G, Martin JF, Wythe JD
  TITLE     Defining the Transcriptional and Epigenetic Basis of Organotypic 
            Endothelial Diversity in the Developing and Adult Mouse
  JOURNAL   bioRxiv 2021.11.15.468651
REFERENCE   2  (bases 1 to 10277)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.11.15.468651"
COMMENT     Sequence Label: JDW 1475 (pLenti-BLAST-GATA4-P2A-H2A-mCherry)
FEATURES             Location/Qualifiers
     source          1..10277
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(176..220)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    405..638
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1131..1256
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1303..1483
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(1484..1710)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(1738..1758)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1738..1756)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1777..1793)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1777..1793)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1790..1812)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1801..1817
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1825..1831,1832..1849,1850..1855))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1870..1891
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2008..2025)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2179..2767)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2259..2278)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2938..3729,3730..3798))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3561..3580
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3799..3903)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3929..4384)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4066..4087)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4278..4297
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4511..4533
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4525..4542
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4526..4542
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4552..4571
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4552..4570
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      4591..4726
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4766..4887
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4777..4796)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4831..4850
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4959..5192
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(5279..5677)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(5696..5743)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(5791..6120)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5805..5940
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6105..6125
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     misc_feature    6176..6764
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             6282..6293
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6691..6711
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    6795..6819
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(6837..7547)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6917..6940)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(7115..7134)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     7384..7402
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(7944..8000)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(9354..9395)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     regulatory      9395..9404
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(9411..9435)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(9519..9543)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(9540..9743)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(9569..9589)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        9744..10047
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    10070..10186
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgggtcccct cggggttggg aggtgggtct gaaacgataa tggtgaatat ccctgcctaa
       61 ctctattcac tatagaaagt acagcaaaaa ctattcttaa acctaccaag cctcctacta
      121 tcattatgaa taattttata taccacagcc aatttgttat gttaaaccaa ttccacaaac
      181 ttgcccattt atctaattcc aataattctt gttcattctt ttcttgctgg ttttgcgatt
      241 cttcaattaa ggagtgtatt aagcttgtgt aattgttaat ttctctgtcc cactccatcc
      301 aggtcgtgtg attccaaatc tgttccagag atttattact ccaactagca ttccaaggca
      361 cagcagtggt gcaaatgagt tttccagagc aaccccaaat ccccaggagc tgttgatcct
      421 ttaggtatct ttccacagcc aggattcttg cctggagctg cttgatgccc cagactgtga
      481 gttgcaacag atgctgttgc gcctcaatag ccctcagcaa attgttctgc tgctgcacta
      541 taccagacaa taattgtctg gcctgtaccg tcagcgtcat tgacgctgcg cccatagtgc
      601 ttcctgctgc tcccaagaac ccaaggaaca aagctcctat tcccactgct cttttttctc
      661 tctgcaccac tcttctcttt gccttggtgg gtgctactcc taatggttca atttttacta
      721 ctttatattt atataattca cttctccaat tgtccctcat atctcctcct ccaggtctga
      781 agatcagcgg ccgcttgctg tgcggtggtc ttacttttgt tttgctcttc ctctatcttg
      841 tctaaagctt ccttggtgtc ttttatctct atcctttgat gcacacaata gagggttgct
      901 actgtattat ataatgatct aagttcttct gatcctgtct gaagggatgg ttgtagctgt
      961 cccagtattt gtctacagcc ttctgatgtt tctaacaggc caggattaac tgcgaatcgt
     1021 tctagctccc tgcttgccca tactatatgt tttaatttat attttttctt tccccctggc
     1081 cttaaccgaa ttttttccca tcgcgatcta attctccccc gcttaatact gacgctctcg
     1141 cacccatctc tctccttcta gcctccgcta gtcaaaattt ttggcgtact caccagtcgc
     1201 cgcccctcgc ctcttgccgt gcgcgcttca gcaagccgag tcctgcgtcg agagagctcc
     1261 tctggtttcc ctttcgcttt caagtccctg ttcgggcgcc actgctagag attttccaca
     1321 ctgactaaaa gggtctgagg gatctctagt taccagagtc acacaacaga cgggcacaca
     1381 ctacttgaag cactcaaggc aagctttatt gaggcttaag cagtgggttc cctagttagc
     1441 cagagagctc ccaggctcag atctggtcta accagagaga cccgtttatg tatcgagcta
     1501 ggcacttaaa tacaatatct ctgcaatgcg gcaattcagt ggttcgtcca atccatgtca
     1561 gacccgtctg ttgccttcct aataaggcac gatcgtacca ccttacttcc accaatcggc
     1621 atgcacggtg ctttttctct ccttgtaagg catgttgcta actcatcgtt accatgttgc
     1681 aagactacaa gagtattgca taagactaca ttaagcttgc agctccagct tttgttccct
     1741 ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     1801 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     1861 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     1921 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     1981 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     2041 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     2101 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     2161 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     2221 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     2281 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     2341 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     2401 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     2461 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     2521 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     2581 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     2641 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     2701 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     2761 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     2821 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     2881 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     2941 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     3001 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     3061 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     3121 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     3181 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     3241 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     3301 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     3361 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     3421 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     3481 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     3541 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     3601 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     3661 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     3721 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     3781 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     3841 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     3901 gcgcacattt ccccgaaaag tgccacctaa attgtaagcg ttaatatttt gttaaaattc
     3961 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     4021 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     4081 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     4141 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     4201 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     4261 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     4321 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     4381 gcgtcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     4441 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
     4501 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac
     4561 tcactatagg gcgaattggg tacgtcccta ggcctccaaa aaagcctcct cactacttct
     4621 ggaatagctc agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc
     4681 catggggcgg agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg
     4741 gcgggatagc tagagccaga catgataaga tacattgatg agtttggaca aaccacaact
     4801 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     4861 accattataa gctgcaataa acaagttcct ctcactctct gatattcatt tctttgcaag
     4921 ttataaatac tgaataataa gatgacatga actactactg ctagagattt tccacactga
     4981 ctaaaagggt ctgagggatc tctagttacc agagtcacac aacagacggg cacacactac
     5041 ttgaagcact caaggcaagc tttattgagg cttaagcagt gggttcccta gttagccaga
     5101 gagctcccag gctcagatct ggtctaacca gagagaccca gtacaagcaa aaagcagatc
     5161 ttgtcttcgt tgggagtgag ctagcccttc cagtcccccc ttttctttta aaaagtggct
     5221 aagatctaca gctgccttgt aagtcattgg tcttaaaggt accgagctcg aattgtgctt
     5281 agccctccca cacataacca gagggcagca attcacgaat cccaactgcc gtcggctgtc
     5341 catcactgtc cttcactatg gctttgatcc caggatgcag atcgagaagc acctgtcggc
     5401 accgtccgca ggggctcaag atgcccctgt tctcatttcc gatcgcgacg atacaagtca
     5461 ggttgccagc tgccgcagca gcagcagtgc ccagcaccac gagttctgca caaggtcccc
     5521 cagtaaaatg atatacattg acaccagtga agatgcggcc gtcgctagag agagctgcgc
     5581 tggcgacgct gtagtcttca gagatgggga tgctgttgat tgtagccgtt gctctttcaa
     5641 tgagggtgga ttcttcttga gacaaaggct tggccatggt ttagttcctc accttgtcgt
     5701 attatactat gccgatatac tatgccgatg attaattgtc aacacgtgct gatcagatcc
     5761 gaaaatggat atacaagctc ccgggagctt tttgcaaaag cctaggcctc caaaaaagcc
     5821 tcctcactac ttctggaata gctcagaggc agaggcggcc tcggcctctg cataaataaa
     5881 aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat
     5941 gggcggagtt aggggcggga ctatggttgc tgactaattg agatgcatgc tttgcatact
     6001 tctgcctgct ggggagcctg gggactttcc acacctggtt gctgactaat tgagatgcat
     6061 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacccta actgacacac
     6121 attccacaga attaattcca aactcattac taaccggata tctgcagaat tccaggcggg
     6181 gaggcggccc aaagggagat ccgactcgtc tgagggcgaa ggcgaagacg cggaagaggc
     6241 cgcagagccg gcagcaggcc gcgggaagga aggtccgctg gattgagggc cgaagggacg
     6301 tagcagaagg acgtcccgcg cagaatccag gtggcaacac aggcgagcag ccatggaaag
     6361 gacgtcagct tccccgacaa caccacggaa ttgtcagtgc ccaacagccg agcccctgtc
     6421 cagcagcggg caaggcaggc ggcgatgagt tccgccgtgg caatagggag ggggaaagcg
     6481 aaagtcccgg aaaggagctg acaggtggtg gcaatgcccc aaccagtggg ggttgcgtca
     6541 gcaaacacag tgcacaccac gccacgttgc ctgacaacgg gccacaactc ctcataaaga
     6601 gacagcaacc aggatttata caaggaggag aaaatgaaag ccatacggga agcaatagca
     6661 tgatacaaag gcattaaagc agcgtatcca catagcgtaa aaggagcaac atagttaaga
     6721 ataccagtca atctttcaca aattttgtaa tccagaggtt gattgtcgag cggccgccac
     6781 tgtgctggat atcaaccact ttgtacaaga aagctgggag atctctcgag ctatcattac
     6841 ttgtacagct cgtccatgcc gccggtggag tggcggccct cggcgcgctc gtactgttcc
     6901 acgatggtgt agtcctcgtt gtgggaggtg atgtccagct tgatgttgac gttgtaggcg
     6961 ccgggcagct gcacgggctt cttggccttg taggtggtct tgacctcggc gtcgtagtgg
     7021 ccgccgtcct tcagcttcag cctctgcttg atctcgccct tcagggcgcc gtcctcgggg
     7081 tacatccgct cggaggaggc ctcccagccc atggtcttct tctgcattac ggggccgtcg
     7141 gaggggaagt tggtgccgcg cagcttcacc ttgtagatga actcgccgtc ctgcagggag
     7201 gagtcctggg tcacggtcac cacgccgccg tcctcgaagt tcatcacgcg ctcccacttg
     7261 aagccctcgg ggaaggacag cttcaagtag tcggggatgt cggcggggtg cttcacgtag
     7321 gccttggagc cgtacatgaa ctgaggggac aggatgtccc aggcgaaggg cagggggccg
     7381 cccttggtca ccttcagctt ggcggtctgg gtgccctcgt aggggcggcc ctcgccctcg
     7441 ccctcgatct cgaactcgtg gccgttcacg gagccctcca tgtgcacctt gaagcgcatg
     7501 aactccttga tgatggccat gttgtcctcc tcgcccttgc tcaccatccc ccctgcggtt
     7561 ttctgctggc ccttcttacc aatcagagac ttgtggatgt gaggaatgac tcctcctcca
     7621 gcaatggtgg ccttgataag cgagtccagc tcctcgtctc cgcggatggc cagctgtaaa
     7681 tgtctgggag tgattcgctt caccttcaga tccttagaag cgtttccagc caactccagc
     7741 acctcagcgg tgagatactc caggatagca gcactgtaaa ccgcagctgt agctccgaca
     7801 cggccatggc tggtggttcg tgtcttcagg tgcctgtgga tacgacctac agggaactgc
     7861 agaccagctc tctgagatct ggagacggct ttcgtcttgg cttttccact gtctttacct
     7921 gcttttccac ctgccatcct aggtggcccg ggattctctt cgacatcccc tgcttgtttc
     7981 aacagggaga agttagtggc tccggtacca cccgcagtga ttatgtcacc atgagaatct
     8041 gcgagcacaa gactattcca actatcttgt tttgaactag tttggggact ttgactcacc
     8101 ggtgaggcgt atccctgggg actcagtttg agtgcggaaa gaactggatg tatgctaggt
     8161 ccgtgaccac tcatggcgct cacactaaag gtttggctta cagatgaaga atgtccataa
     8221 tgggaactaa gtcccggttc agtctttata ggtcgcattt cttcagaaga agatgtcgta
     8281 gcatttgatg aattacttga tgctcctgat gccggtggca gactttcgga cccggacggt
     8341 gcggcaggag ttttgctctt gttgagattc ttcggtttcc tcttgcgagt ctgaatgcct
     8401 tccttacgca tggccagcgg ccgaggaact ccatgcagtt tcatatacag tccacaagcg
     8461 ttacagactg gttccccttc ggcgtttctt ctccacaagg ttgttgtagt tgtttgacaa
     8521 tttgcgcaac tcagacctac ccgccgactc gctgacaagc gacgttgggg ttttatcagt
     8581 ggacgattta ttccattcat tttatgatac agtccacaag cattacacaa gtaatgtcct
     8641 gtgccgtcgc gacgccacaa gggagttgac atcgctccgc aattcacgca ttcgcgcccc
     8701 tcgctaaaat catcgaacat gtccaggttt ggatgcctcg ctgcgggatt cgctctccct
     8761 ggcagggaat gcaacacggg agaatcaaat ggaccagcgc tagctgctgc agcggcagcc
     8821 catgaggctc cgacatctgc catataggcg ggataaggtg aactataaga ccctgcaaac
     8881 cccgctcttc catattgttc acgcccagcg agaccagccc cggcggcccc accacctgat
     8941 gaatatgcgg cggcctctct agcagccgcg gcagcagcag ctgcggcgag gctaccagta
     9001 gtaccgggaa agctaaaccg aggggagact ggtggtggcg tataggctgc accatcagcc
     9061 ccagcttgac tccagcctgg ggacccttgt tgtgttccag gaccagcccc gctagcagcg
     9121 ccgccagatg atccgccact agctccaccg cttgcactcc cggccccgcc accttgcaaa
     9181 taagaaagcc caagaacgct tgatggtacg cgtggagttg gtacataaac aggggaacta
     9241 gcagctccgg caccatgcat aaatgcacca ggtccgccgg cttcatacgc tccaggtggt
     9301 ggtccatgat tagctgccat cgcgagggat tggtacatac caccagcacc acctgttgag
     9361 tcaaggccca gcaagggatt tgggattggt ttacccatgg tggcggatcc agcctgcttt
     9421 tttgtacaaa cttgttgata tctgcagaat tccaccacac tggactagag tcggtgtctt
     9481 ctatggaggt caaaacagcg tggatggcgt ctccaggcga tctgacggtt cactaaacga
     9541 gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg
     9601 gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg
     9661 acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg
     9721 atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc
     9781 caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg
     9841 tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg
     9901 gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact
     9961 atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc
    10021 gggccattta ccgtaagtta tgtaacgcgg aactcccaag cttatcgata aaattttgaa
    10081 ttttgtaatt tgtttttgta attctttagt ttgtatgtct gttgctatta tgtctactat
    10141 tctttcccct gcactgtacc ccccaatccc cccttttctt ttaaaagtta accgataccg
    10201 tcgagatccg ttcactaatc gaatggatct gtctctgtct ctctctccac cttcttcttc
    10261 tattccttcg ggcctgt
//
