LOCUS       sequence-462777-       10349 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A CMV driven human ZIC3 followed by a P2A cleavage peptide and an 
            H2A mCherry fusion protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10349)
  AUTHORS   Cantu Gutierrez ME, Hill MC, Largoza G, Martin JF, Wythe JD
  TITLE     Defining the Transcriptional and Epigenetic Basis of Organotypic 
            Endothelial Diversity in the Developing and Adult Mouse
  JOURNAL   bioRxiv 2021.11.15.468651
REFERENCE   2  (bases 1 to 10349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.11.15.468651"
COMMENT     Sequence Label: JDW 1473 (pLenti-BLAST-ZIC3-P2A-H2A-mCherry)
FEATURES             Location/Qualifiers
     source          1..10349
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(183..194)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(351..371)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        356..685
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      536..671
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        733..780
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             799..1197
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             1284..1517
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1589..1710
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1626..1645)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1680..1699
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1750..1885
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1905..1924)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1906..1924)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1934..1951)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1934..1950)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1943..1965)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2092..2547
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2179..2198)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2389..2410
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2573..2677
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2678..2746,2747..3538)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2896..2915)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3709..4297
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4198..4217
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4451..4468
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4585..4606
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4621..4626,4627..4644,4645..4651)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4659..4675
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4664..4686
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4683..4699
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4683..4699
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4718..4738
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4720..4738
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4766..4992
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4993..5173
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5220..5345
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5838..6071
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6256..6300
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6567..6683
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        6706..7009
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7010..7213
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7164..7184
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7210..7234
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    7318..7342
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      7349..7358
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7358..7399
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             7673..7702
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             7676..7705
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             8825..8881
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             9278..9988
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(9423..9441)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     9691..9710
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     9885..9908
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     protein_bind    complement(10006..10030)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(10061..10349,1..300)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10114..10134)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
       61 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc
      121 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
      181 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
      241 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
      301 ctggaattct gcagatatcc ggttagtaat gagtttggaa ttaattctgt ggaatgtgtg
      361 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
      421 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
      481 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
      541 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
      601 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
      661 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
      721 tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac
      781 aaggtgagga actaaaccat ggccaagcct ttgtctcaag aagaatccac cctcattgaa
      841 agagcaacgg ctacaatcaa cagcatcccc atctctgaag actacagcgt cgccagcgca
      901 gctctctcta gcgacggccg catcttcact ggtgtcaatg tatatcattt tactggggga
      961 ccttgtgcag aactcgtggt gctgggcact gctgctgctg cggcagctgg caacctgact
     1021 tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg acggtgccga
     1081 caggtgcttc tcgatctgca tcctgggatc aaagccatag tgaaggacag tgatggacag
     1141 ccgacggcag ttgggattcg tgaattgctg ccctctggtt atgtgtggga gggctaagca
     1201 caattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     1261 ctttttaaaa gaaaaggggg gactggaagg gctagctcac tcccaacgaa gacaagatct
     1321 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     1381 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     1441 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     1501 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     1561 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     1621 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     1681 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     1741 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     1801 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     1861 tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt
     1921 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     1981 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     2041 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct
     2101 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     2161 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     2221 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     2281 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     2341 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     2401 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     2461 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     2521 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac
     2581 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     2641 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     2701 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     2761 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     2821 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     2881 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     2941 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     3001 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     3061 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     3121 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     3181 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     3241 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     3301 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     3361 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     3421 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     3481 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     3541 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     3601 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     3661 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     3721 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     3781 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     3841 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     3901 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     3961 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     4021 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     4081 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     4141 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     4201 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     4261 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     4321 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     4381 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     4441 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     4501 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     4561 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     4621 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     4681 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga
     4741 acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac
     4801 atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga
     4861 ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca
     4921 tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct
     4981 cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     5041 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     5101 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     5161 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
     5221 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
     5281 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
     5341 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
     5401 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
     5461 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
     5521 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
     5581 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     5641 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
     5701 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
     5761 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
     5821 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
     5881 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
     5941 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
     6001 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
     6061 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
     6121 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
     6181 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
     6241 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
     6301 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
     6361 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
     6421 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
     6481 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
     6541 aacggatctc gacggtatcg gttaactttt aaaagaaaag gggggattgg ggggtacagt
     6601 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     6661 caaattacaa aattcaaaat tttatcgata agcttgggag ttccgcgtta cataacttac
     6721 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     6781 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     6841 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     6901 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     6961 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     7021 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     7081 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     7141 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     7201 tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt
     7261 tgacctccat agaagacacc gactctagtc cagtgtggtg gaattctgca gatatcaaca
     7321 agtttgtaca aaaaagcagg ctggatccgc caccatggga aaacctatac caaatccctt
     7381 gctgggcctg gactcaacag gtggtgctgg tggtatgacg atgctcctgg acggaggccc
     7441 gcagttccct gggctgggag tgggcagctt cggcgcgccg cgccaccacg agatgcccaa
     7501 ccgtgagccg gcaggcatgg ggctgaatcc cttcggggac tcaacccacg ccgccgccgc
     7561 cgccgccgcc gccgctgcct tcaagctgag ccctgccgcg gcgcacgatc tatcttcagg
     7621 ccagagctcg gctttcacgc cgcagggttc gggctacgcc aacgccctgg gccaccatca
     7681 ccaccaccat caccatcatc accacaccag ccaggtgccc agctacggtg gcgctgcctc
     7741 tgccgccttc aactcaacgc gcgagtttct gttccgccag cgcagctccg ggctcagtga
     7801 ggcggcctcg ggtggcgggc agcacgggct cttcgccggc tcggcgagca gcctgcatgc
     7861 tccagctggc atccccgagc cccctagcta cttgctgttt cccgggctgc atgagcaggg
     7921 cgctgggcac ccgtcgccca cagggcacgt ggacaacaac caggtccacc tggggctgcg
     7981 tggggagctg ttcggccgtg ctgacccata ccgcccagtg gccagcccgc gcacggaccc
     8041 ctacgcggcc ggcgctcagt ttcctaacta cagccccatg aacatgaaca tgggagtgaa
     8101 cgtggcggcc caccacgggc ccggcgcctt cttccgttat atgcggcagc ctatcaagca
     8161 ggagctgtcg tgcaagtgga tcgacgaggc tcagctgagc cggcccaaga agagctgcga
     8221 ccggaccttc agcaccatgc atgagctggt gacacatgtc accatggagc atgtgggggg
     8281 cccggagcag aacaaccacg tctgctactg ggaggagtgc ccccgggagg gcaagtcttt
     8341 caaggcgaag tacaaactgg tcaaccacat ccgagtgcac acgggcgaga agcccttccc
     8401 atgccccttc ccgggctgcg ggaagatctt tgcccgttct gagaacctca agatccacaa
     8461 gaggacccac acaggtgaga aacctttcaa atgtgaattt gaaggctgtg acagacgctt
     8521 tgccaacagc agcgaccgta agaagcacat gcatgtgcat acctcggaca agccctatat
     8581 ctgcaaagtg tgcgacaagt cctacacgca cccgagctcc ctgcgcaaac acatgaaggt
     8641 tcatgaatct caagggtcag attcctcccc tgctgccagt tcaggctatg aatcttccac
     8701 tccacccgct atagcttctg caaacagtaa agataccact aaaacccctt ctgcagttca
     8761 aactagcacc agccacaacc ctggacttcc tcctaatttt aacgaatggt acgtcggtac
     8821 cggagccact aacttctccc tgttgaaaca agcaggggat gtcgaagaga atcccgggcc
     8881 acctaggatg gcaggtggaa aagcaggtaa agacagtgga aaagccaaga cgaaagccgt
     8941 ctccagatct cagagagctg gtctgcagtt ccctgtaggt cgtatccaca ggcacctgaa
     9001 gacacgaacc accagccatg gccgtgtcgg agctacagct gcggtttaca gtgctgctat
     9061 cctggagtat ctcaccgctg aggtgctgga gttggctgga aacgcttcta aggatctgaa
     9121 ggtgaagcga atcactccca gacatttaca gctggccatc cgcggagacg aggagctgga
     9181 ctcgcttatc aaggccacca ttgctggagg aggagtcatt cctcacatcc acaagtctct
     9241 gattggtaag aagggccagc agaaaaccgc aggggggatg gtgagcaagg gcgaggagga
     9301 caacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa
     9361 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
     9421 cgccaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc
     9481 tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt
     9541 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
     9601 cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa
     9661 gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg
     9721 ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca
     9781 gaggctgaag ctgaaggacg gcggccacta cgacgccgag gtcaagacca cctacaaggc
     9841 caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagctgg acatcacctc
     9901 ccacaacgag gactacacca tcgtggaaca gtacgagcgc gccgagggcc gccactccac
     9961 cggcggcatg gacgagctgt acaagtaatg atagctcgag agatctccca gctttcttgt
    10021 acaaagtggt tgatatccag cacagtggcg gccgctcgac aatcaacctc tggattacaa
    10081 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
    10141 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
    10201 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
    10261 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
    10321 ctgtcagctc ctttccggga ctttcgctt
//
