LOCUS       sequence-462778-        9459 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A CMV driven human MAFB followed by a P2A cleavage peptide and an 
            H2A mCherry fusion protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9459)
  AUTHORS   Cantu Gutierrez ME, Hill MC, Largoza G, Martin JF, Wythe JD
  TITLE     Defining the Transcriptional and Epigenetic Basis of Organotypic 
            Endothelial Diversity in the Developing and Adult Mouse
  JOURNAL   bioRxiv 2021.11.15.468651
REFERENCE   2  (bases 1 to 9459)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.11.15.468651"
COMMENT     Sequence Label: JDW 1063 (pLenti6.2_CMV_V5_MAFB_P2A_H2A_mCherry)
FEATURES             Location/Qualifiers
     source          1..9459
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    722..847
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             894..1074
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(1075..1301)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(1329..1349)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1329..1347)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1368..1384)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1368..1384)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1381..1403)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1392..1408
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1416..1422,1423..1440,1441..1446))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1461..1482
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1599..1616)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1770..2358)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1850..1869)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2529..3320,3321..3389))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3152..3171
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3390..3494)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3520..3975)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3657..3678)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3869..3888
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4102..4124
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4116..4133
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4117..4133
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4143..4162
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4143..4161
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      4182..4317
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4357..4478
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4368..4387)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4422..4441
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4550..4783
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(4870..5268)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(5287..5334)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(5348..5847)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5396..5415)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     5789..5812
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     CDS             complement(5880..5921)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    5974..5998
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(6016..6726)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6096..6119)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(6294..6313)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     6563..6581
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(7123..7179)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(7660..7677)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(7747..7770)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(8176..8217)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     regulatory      8217..8226
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(8233..8257)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(8353..8377)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(8374..8577)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(8403..8423)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        8578..8881
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(9226..9270)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    join(9455..9459,1..229)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 ctgttgatcc tttaggtatc tttccacagc caggattctt gcctggagct gcttgatgcc
       61 ccagactgtg agttgcaaca gatgctgttg cgcctcaata gccctcagca aattgttctg
      121 ctgctgcact ataccagaca ataattgtct ggcctgtacc gtcagcgtca ttgacgctgc
      181 gcccatagtg cttcctgctg ctcccaagaa cccaaggaac aaagctccta ttcccactgc
      241 tcttttttct ctctgcacca ctcttctctt tgccttggtg ggtgctactc ctaatggttc
      301 aatttttact actttatatt tatataattc acttctccaa ttgtccctca tatctcctcc
      361 tccaggtctg aagatcagcg gccgcttgct gtgcggtggt cttacttttg ttttgctctt
      421 cctctatctt gtctaaagct tccttggtgt cttttatctc tatcctttga tgcacacaat
      481 agagggttgc tactgtatta tataatgatc taagttcttc tgatcctgtc tgaagggatg
      541 gttgtagctg tcccagtatt tgtctacagc cttctgatgt ttctaacagg ccaggattaa
      601 ctgcgaatcg ttctagctcc ctgcttgccc atactatatg ttttaattta tattttttct
      661 ttccccctgg ccttaaccga attttttccc atcgcgatct aattctcccc cgcttaatac
      721 tgacgctctc gcacccatct ctctccttct agcctccgct agtcaaaatt tttggcgtac
      781 tcaccagtcg ccgcccctcg cctcttgccg tgcgcgcttc agcaagccga gtcctgcgtc
      841 gagagagctc ctctggtttc cctttcgctt tcaagtccct gttcgggcgc cactgctaga
      901 gattttccac actgactaaa agggtctgag ggatctctag ttaccagagt cacacaacag
      961 acgggcacac actacttgaa gcactcaagg caagctttat tgaggcttaa gcagtgggtt
     1021 ccctagttag ccagagagct cccaggctca gatctggtct aaccagagag acccgtttat
     1081 gtatcgagct aggcacttaa atacaatatc tctgcaatgc ggcaattcag tggttcgtcc
     1141 aatccatgtc agacccgtct gttgccttcc taataaggca cgatcgtacc accttacttc
     1201 caccaatcgg catgcacggt gctttttctc tccttgtaag gcatgttgct aactcatcgt
     1261 taccatgttg caagactaca agagtattgc ataagactac attaagcttg cagctccagc
     1321 ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt
     1381 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     1441 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     1501 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     1561 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     1621 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     1681 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     1741 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     1801 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     1861 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     1921 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     1981 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     2041 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     2101 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     2161 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     2221 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     2281 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     2341 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     2401 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     2461 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     2521 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     2581 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     2641 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     2701 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     2761 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     2821 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     2881 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     2941 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     3001 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     3061 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     3121 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     3181 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     3241 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     3301 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     3361 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     3421 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     3481 ataggggttc cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc gttaatattt
     3541 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
     3601 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
     3661 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     3721 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
     3781 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     3841 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
     3901 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     3961 cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
     4021 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
     4081 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg
     4141 cgtaatacga ctcactatag ggcgaattgg gtacgtccct aggcctccaa aaaagcctcc
     4201 tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat aaataaaaaa
     4261 aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg
     4321 cggagttagg ggcgggatag ctagagccag acatgataag atacattgat gagtttggac
     4381 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     4441 ctttatttgt aaccattata agctgcaata aacaagttcc tctcactctc tgatattcat
     4501 ttctttgcaa gttataaata ctgaataata agatgacatg aactactact gctagagatt
     4561 ttccacactg actaaaaggg tctgagggat ctctagttac cagagtcaca caacagacgg
     4621 gcacacacta cttgaagcac tcaaggcaag ctttattgag gcttaagcag tgggttccct
     4681 agttagccag agagctccca ggctcagatc tggtctaacc agagagaccc agtacaagca
     4741 aaaagcagat cttgtcttcg ttgggagtga attagccctt ccagtccccc cttttctttt
     4801 aaaaagtggc taagatctac agctgccttg taagtcattg gtcttaaagg taccgagctc
     4861 gaattgtgct tagccctccc acacataacc agagggcagc aattcacgaa tcccaactgc
     4921 cgtcggctgt ccatcactgt ccttcactat ggctttgatc ccaggatgca gatcgagaag
     4981 cacctgtcgg caccgtccgc aggggctcaa gatgcccctg ttctcatttc cgatcgcgac
     5041 gatacaagtc aggttgccag ctgccgcagc agcagcagtg cccagcacca cgagttctgc
     5101 acaaggtccc ccagtaaaat gatatacatt gacaccagtg aagatgcggc cgtcgctaga
     5161 gagagctgcg ctggcgacgc tgtagtcttc agagatgggg atgctgttga ttgtagccgt
     5221 tgctctttca atgagggtgg attcttcttg agacaaaggc ttggccatgg tttagttcct
     5281 caccttgtcg tattatacta tgccgatata ctatgccgat gattaattgt caacacgtgt
     5341 ctagagtcga aaggcccgga gatgaggaag aggagaacag cgcggcagac gtgcgctttt
     5401 gaagcgtgca gaatgccggg cctccggagg accttcgggc gcccgccccg cccctgagcc
     5461 cgcccctgag cccgcccccg gacccacccc ttcccagcct ctgagcccag aaagcgaagg
     5521 agcaaagctg ctattggccg ctgccccaaa ggcctacccg cttccattgc tcagcggtgc
     5581 tgtccatctg cacgagacta gtgagacgtg ctacttccat ttgtcacgtc ctgcacgacg
     5641 cgagctgcgg ggcggggggg aacttcctga ctaggggagg agtagaaggt ggcgcgaagg
     5701 ggccaccaaa gaacggagcc ggttggcgcc taccggtgga tgtggaatgt gtgcgaggcc
     5761 agaggccact tgtgtagcgc caagtgccca gcggggctgc taaagcgcat gctccagact
     5821 gccttgggaa aagcgcctcc cctacccggt aggtaccatc gtcattacta accggtacgc
     5881 gtagaatcga gaccgaggag agggttaggg ataggcttac cttcgaaccg cgggccctct
     5941 agactcgagc ggccgccact gtgctggata tcaaccactt tgtacaagaa agctgggaga
     6001 tctctcgagc tatcattact tgtacagctc gtccatgccg ccggtggagt ggcggccctc
     6061 ggcgcgctcg tactgttcca cgatggtgta gtcctcgttg tgggaggtga tgtccagctt
     6121 gatgttgacg ttgtaggcgc cgggcagctg cacgggcttc ttggccttgt aggtggtctt
     6181 gacctcggcg tcgtagtggc cgccgtcctt cagcttcagc ctctgcttga tctcgccctt
     6241 cagggcgccg tcctcggggt acatccgctc ggaggaggcc tcccagccca tggtcttctt
     6301 ctgcattacg gggccgtcgg aggggaagtt ggtgccgcgc agcttcacct tgtagatgaa
     6361 ctcgccgtcc tgcagggagg agtcctgggt cacggtcacc acgccgccgt cctcgaagtt
     6421 catcacgcgc tcccacttga agccctcggg gaaggacagc ttcaagtagt cggggatgtc
     6481 ggcggggtgc ttcacgtagg ccttggagcc gtacatgaac tgaggggaca ggatgtccca
     6541 ggcgaagggc agggggccgc ccttggtcac cttcagcttg gcggtctggg tgccctcgta
     6601 ggggcggccc tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg agccctccat
     6661 gtgcaccttg aagcgcatga actccttgat gatggccatg ttgtcctcct cgcccttgct
     6721 caccatcccc cctgcggttt tctgctggcc cttcttacca atcagagact tgtggatgtg
     6781 aggaatgact cctcctccag caatggtggc cttgataagc gagtccagct cctcgtctcc
     6841 gcggatggcc agctgtaaat gtctgggagt gattcgcttc accttcagat ccttagaagc
     6901 gtttccagcc aactccagca cctcagcggt gagatactcc aggatagcag cactgtaaac
     6961 cgcagctgta gctccgacac ggccatggct ggtggttcgt gtcttcaggt gcctgtggat
     7021 acgacctaca gggaactgca gaccagctct ctgagatctg gagacggctt tcgtcttggc
     7081 ttttccactg tctttacctg cttttccacc tgccatccta ggtggcccgg gattctcttc
     7141 gacatcccct gcttgtttca acagggagaa gttagtggct ccggtaccgc acagaaagaa
     7201 ctcgggagag gaggggctgt cgctggtgga gcccgcctcc ctgaagccgg agttggcgag
     7261 tttctcgcac ttgaccttgt aggcgtctct ctcgcgggcc agccgggaca cctcctgctt
     7321 aagctgctcc acctgctgaa tgagctgcgt cttctcattc tccaggtggt gcttctgctg
     7381 gacgcgttta tacctgcaag actgggcgta gccccggttc ttcagggtcc gccgcttctg
     7441 cttcaggcgg atcacctcgt ccttggtgaa gccccgcagg tggcggttca gctcgcgcac
     7501 ggacatggac acgagctggt catcggagaa gcggtcctcc acgctgccgt tgccgcccgc
     7561 cgccgtcgcc gaggccgtcg cgtgcggccc gggcccgggg tggctagtgg gcagctgttg
     7621 cgccgggcta gcggcgctgg acggcggcgg cgacgcttgg tgatgatggt gatggtgcgg
     7681 gtgagcgtgc gggcccagct cgtcgtgggc cacgccggcg cccgggtacg cgtggtgcgg
     7741 gtgagggtgg tggtgatggt ggtggtggtg agcgccgcga aagctgtcga agctttgcag
     7801 cggctgtggc actgggtgcg agccgatgag cgcttccacc gcgtcctcgg gcgtcaggtt
     7861 gagcgcctcg gggttcatct gctggtagtt gctcgccatc cagtacagat cctcgaggtg
     7921 tgtcttctgt tcggtcgggc tgaagctggg cgacgagggc acggagctac acggagtgct
     7981 gagcggtgtg gaggacaccg agccggctgg ctgcaggcgt gtgcagggcc tgcccggacg
     8041 ctccgcgcgc cccagtggct ccttcttcac gtcgaacttg agcaggtcga agtcgttgac
     8101 atactccatg gccagcgggc tggtgggcag ctctggcccc atgctcagct ccgcggccat
     8161 accaccagca ccacccgtag aatcgagacc gaggagaggg ttagggatag gcttacccat
     8221 ggtggcggat ccagcctgct tttttgtaca aacttgttga tatctgcaga attccaccac
     8281 actggactag tggatcctct agagtcggtg tcttctatgg aggtcaaaac agcgtggatg
     8341 gcgtctccag gcgatctgac ggttcactaa acgagctctg cttatataga cctcccaccg
     8401 tacacgccta ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc
     8461 ccgttgattt tggtgccaaa acaaactccc attgacgtca atggggtgga gacttggaaa
     8521 tccccgtgag tcaaaccgct atccacgccc attgatgtac tgccaaaacc gcatcaccat
     8581 ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc ataaggtcat
     8641 gtactgggca taatgccagg cgggccattt accgtcattg acgtcaatag ggggcgtact
     8701 tggcatatga tacacttgat gtactgccaa gtgggcagtt taccgtaaat actccaccca
     8761 ttgacgtcaa tggaaagtcc ctattggcgt tactatggga acatacgtca ttattgacgt
     8821 caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac
     8881 gcggaactcc caagcttatc gcagacccag tcgctagcgt taacgtcgac atcgcagacc
     8941 cagtcgctag cgttaacgtc gacatcgata ccgtcgagat ccgttcacta atcgaatgga
     9001 tctgtctctg tctctctctc caccttcttc ttctattcct tcgggcctgt cgggtcccct
     9061 cggggttggg aggtgggtct gaaacgataa tggtgaatat ccctgcctaa ctctattcac
     9121 tatagaaagt acagcaaaaa ctattcttaa acctaccaag cctcctacta tcattatgaa
     9181 taattttata taccacagcc aatttgttat gttaaaccaa ttccacaaac ttgcccattt
     9241 atctaattcc aataattctt gttcattctt ttcttgctgg ttttgcgatt cttcaattaa
     9301 ggagtgtatt aagcttgtgt aattgttaat ttctctgtcc cactccatcc aggtcgtgtg
     9361 attccaaatc tgttccagag atttattact ccaactagca ttccaaggca cagcagtggt
     9421 gcaaatgagt tttccagagc aaccccaaat ccccaggag
//
