LOCUS       sequence-462780-        9495 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A CMV driven human GATA1 followed by a P2A cleavage peptide and an 
            H2A mCherry fusion protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9495)
  AUTHORS   Cantu Gutierrez ME, Hill MC, Largoza G, Martin JF, Wythe JD
  TITLE     Defining the Transcriptional and Epigenetic Basis of Organotypic 
            Endothelial Diversity in the Developing and Adult Mouse
  JOURNAL   bioRxiv 2021.11.15.468651
REFERENCE   2  (bases 1 to 9495)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.11.15.468651"
COMMENT     Sequence Label: JDW 1061 (pLenti6.2_CMV_GATA1_P2A_H2A_mCherry)
FEATURES             Location/Qualifiers
     source          1..9495
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             29..209
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(210..436)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(464..484)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(464..482)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(503..519)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(503..519)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(516..538)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    527..543
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(551..557,558..575,576..581))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    596..617
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(734..751)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(905..1493)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(985..1004)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1664..2455,2456..2524))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2287..2306
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2525..2629)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2655..3110)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2792..2813)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3004..3023
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3237..3259
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3251..3268
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3252..3268
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3278..3297
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3278..3296
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      3317..3452
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3492..3613
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3503..3522)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3557..3576
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             3685..3918
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(4005..4403)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(4422..4469)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(4483..4982)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4531..4550)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     4924..4947
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     CDS             complement(5015..5056)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    5109..5133
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(5151..5861)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5231..5254)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(5429..5448)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5698..5716
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(6258..6314)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(7347..7388)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     regulatory      7388..7397
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(7404..7428)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(7524..7548)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(7545..7748)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(7574..7594)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        7749..8052
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(8397..8441)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8626..8859
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    9352..9477
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 cgctttcaag tccctgttcg ggcgccactg ctagagattt tccacactga ctaaaagggt
       61 ctgagggatc tctagttacc agagtcacac aacagacggg cacacactac ttgaagcact
      121 caaggcaagc tttattgagg cttaagcagt gggttcccta gttagccaga gagctcccag
      181 gctcagatct ggtctaacca gagagacccg tttatgtatc gagctaggca cttaaataca
      241 atatctctgc aatgcggcaa ttcagtggtt cgtccaatcc atgtcagacc cgtctgttgc
      301 cttcctaata aggcacgatc gtaccacctt acttccacca atcggcatgc acggtgcttt
      361 ttctctcctt gtaaggcatg ttgctaactc atcgttacca tgttgcaaga ctacaagagt
      421 attgcataag actacattaa gcttgcagct ccagcttttg ttccctttag tgagggttaa
      481 ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
      541 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
      601 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
      661 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
      721 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
      781 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
      841 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
      901 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
      961 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     1021 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     1081 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     1141 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     1201 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     1261 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     1321 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     1381 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     1441 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     1501 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     1561 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     1621 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     1681 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     1741 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     1801 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     1861 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     1921 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     1981 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     2041 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     2101 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     2161 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     2221 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     2281 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     2341 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     2401 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     2461 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     2521 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     2581 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     2641 gaaaagtgcc acctaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
     2701 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     2761 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     2821 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     2881 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     2941 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     3001 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     3061 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc
     3121 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
     3181 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
     3241 gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac tatagggcga
     3301 attgggtacg tccctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag
     3361 gccgaggcgg cctcggcctc tgcataaata aaaaaaatta gtcagccatg gggcggagaa
     3421 tgggcggaac tgggcggagt taggggcggg atgggcggag ttaggggcgg gatagctaga
     3481 gccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     3541 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     3601 caataaacaa gttcctctca ctctctgata ttcatttctt tgcaagttat aaatactgaa
     3661 taataagatg acatgaacta ctactgctag agattttcca cactgactaa aagggtctga
     3721 gggatctcta gttaccagag tcacacaaca gacgggcaca cactacttga agcactcaag
     3781 gcaagcttta ttgaggctta agcagtgggt tccctagtta gccagagagc tcccaggctc
     3841 agatctggtc taaccagaga gacccagtac aagcaaaaag cagatcttgt cttcgttggg
     3901 agtgaattag cccttccagt cccccctttt cttttaaaaa gtggctaaga tctacagctg
     3961 ccttgtaagt cattggtctt aaaggtaccg agctcgaatt gtgcttagcc ctcccacaca
     4021 taaccagagg gcagcaattc acgaatccca actgccgtcg gctgtccatc actgtccttc
     4081 actatggctt tgatcccagg atgcagatcg agaagcacct gtcggcaccg tccgcagggg
     4141 ctcaagatgc ccctgttctc atttccgatc gcgacgatac aagtcaggtt gccagctgcc
     4201 gcagcagcag cagtgcccag caccacgagt tctgcacaag gtcccccagt aaaatgatat
     4261 acattgacac cagtgaagat gcggccgtcg ctagagagag ctgcgctggc gacgctgtag
     4321 tcttcagaga tggggatgct gttgattgta gccgttgctc tttcaatgag ggtggattct
     4381 tcttgagaca aaggcttggc catggtttag ttcctcacct tgtcgtatta tactatgccg
     4441 atatactatg ccgatgatta attgtcaaca cgtgtctaga gtcgaaaggc ccggagatga
     4501 ggaagaggag aacagcgcgg cagacgtgcg cttttgaagc gtgcagaatg ccgggcctcc
     4561 ggaggacctt cgggcgcccg ccccgcccct gagcccgccc ctgagcccgc cccccgaccc
     4621 accccctccc cgcccctgag ccccgaaagc gaaggagcaa agctgctatt ggccgctgcc
     4681 ccaaaggcct acccccttcc attgctcagc ggtgctgtcc atctgcacga gactagtgag
     4741 acgtgctact tccatttgtc acgtcctgca cgacgcgagc tgcggggcgg gggggaactt
     4801 cctgactagg ggaggagtag aaggtggcgc gaaggggcca ccaaagaacg gagccggttg
     4861 gcgcctaccg gtggatgtgg aatgtgtgcg aggccagagg ccacttgtgt agcgccaagt
     4921 gcccagcggg gctgctaaag cgcatgctcc agactgcctt gggaaaagcg cctcccctac
     4981 ccggtaggta ccatcgtcat tactaaccgg tacgcgtaga atcgagaccg aggagagggt
     5041 tagggatagg cttaccttcg aaccgcgggc cctctagact cgagcggccg ccactgtgct
     5101 ggatatcaac cactttgtac aagaaagctg ggagatctct cgagctatca ttacttgtac
     5161 agctcgtcca tgccgccggt ggagtggcgg ccctcggcgc gctcgtactg ttccacgatg
     5221 gtgtagtcct cgttgtggga ggtgatgtcc agcttgatgt tgacgttgta ggcgccgggc
     5281 agctgcacgg gcttcttggc cttgtaggtg gtcttgacct cggcgtcgta gtggccgccg
     5341 tccttcagct tcagcctctg cttgatctcg cccttcaggg cgccgtcctc ggggtacatc
     5401 cgctcggagg aggcctccca gcccatggtc ttcttctgca ttacggggcc gtcggagggg
     5461 aagttggtgc cgcgcagctt caccttgtag atgaactcgc cgtcctgcag ggaggagtcc
     5521 tgggtcacgg tcaccacgcc gccgtcctcg aagttcatca cgcgctccca cttgaagccc
     5581 tcggggaagg acagcttcaa gtagtcgggg atgtcggcgg ggtgcttcac gtaggccttg
     5641 gagccgtaca tgaactgagg ggacaggatg tcccaggcga agggcagggg gccgcccttg
     5701 gtcaccttca gcttggcggt ctgggtgccc tcgtaggggc ggccctcgcc ctcgccctcg
     5761 atctcgaact cgtggccgtt cacggagccc tccatgtgca ccttgaagcg catgaactcc
     5821 ttgatgatgg ccatgttgtc ctcctcgccc ttgctcacca tcccccctgc ggttttctgc
     5881 tggcccttct taccaatcag agacttgtgg atgtgaggaa tgactcctcc tccagcaatg
     5941 gtggccttga taagcgagtc cagctcctcg tctccgcgga tggccagctg taaatgtctg
     6001 ggagtgattc gcttcacctt cagatcctta gaagcgtttc cagccaactc cagcacctca
     6061 gcggtgagat actccaggat agcagcactg taaaccgcag ctgtagctcc gacacggcca
     6121 tggctggtgg ttcgtgtctt caggtgcctg tggatacgac ctacagggaa ctgcagacca
     6181 gctctctgag atctggagac ggctttcgtc ttggcttttc cactgtcttt acctgctttt
     6241 ccacctgcca tcctaggtgg cccgggattc tcttcgacat cccctgcttg tttcaacagg
     6301 gagaagttag tggctccggt accgcaagcc agggggtgcc tggggcccag agacttgggt
     6361 tgtccagaat tctggctaca agaggagaag gacaccaccc ctcctctgga ggccatgctc
     6421 tgtgccctca tgagctgagc ggagccacca cagtagtgct ggtggtgtag cttgtagtag
     6481 aggccgcagg cattgcacac gggatcccca ctggcatttc tccgccacag tgtcgtggtg
     6541 gtcgtctggc agttggtgca ctgagtacct gcccgtttac tgacaatcag gcgcttcttg
     6601 ggccggatga ggggcctgtt ctgcccattc atcttgtgat agaggccgca ggcgttgcat
     6661 aggtagtggc ctgtcctgtc cctccgccac agtggagtgg ctgttgctcc gcagttcaca
     6721 cactccctgg cctcacaggg aggcaggggg agagttccac gaagcttggg agaggaatag
     6781 gctgctgaat tgagggggct cccggtggga gaaaagaagg tactggaaaa gtcagggccc
     6841 ccataagcac tattggggac agggagtgat gaaggcagtg caggtcccag ggtcaggagg
     6901 tctgggctca gccgctctgt cttcaaagtc tccaggaagc tggtgctgcc ttttccatcc
     6961 agatcttcca cggcctgggg aggagagtcc tcgcgggtgg gacacacagt tgaggcaggg
     7021 tagagccccg tcttgccgta ggcccagccg gcatatggtg agccccctgg gatcccctcc
     7081 atacagttga gcaatgggta cacctgaaag actggggagt gtctgtaggc ctcagcgtcc
     7141 ctgtagtagg ccagtgccgc agctgcagcg gtggctgtgc tcggggcagt ggaggaagct
     7201 gctgcatcca agccctcagg cccagagggg aagaaaaccc ctgattctgg tgtggaggac
     7261 accagagcag gatccacaaa ctgggggagg ggctctgagg tccccaggga ccccaggcca
     7321 gggaactcca taccaccagc accacccgta gaatcgagac cgaggagagg gttagggata
     7381 ggcttaccca tggtggctga tccagcctgc ttttttgtac aaacttgttg atatctgcag
     7441 aattccacca cactggacta gtggatcctc tagagtcggt gtcttctatg gaggtcaaaa
     7501 cagcgtggat ggcgtctcca ggcgatctga cggttcacta aacgagctct gcttatatag
     7561 acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt gttacgacat
     7621 tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc aatggggtgg
     7681 agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac
     7741 cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc
     7801 cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata
     7861 gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa
     7921 tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg aacatacgtc
     7981 attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc atttaccgta
     8041 agttatgtaa cgcggaactc ccaagcttat cgcagaccca gtcgctagcg ttaacgtcga
     8101 catcgcagac ccagtcgcta gcgttaacgt cgacatcgat accgtcgaga tccgttcact
     8161 aatcgaatgg atctgtctct gtctctctct ccaccttctt cttctattcc ttcgggcctg
     8221 tcgggtcccc tcggggttgg gaggtgggtc tgaaacgata atggtgaata tccctgccta
     8281 actctattca ctatagaaag tacagcaaaa actattctta aacctaccaa gcctcctact
     8341 atcattatga ataattttat ataccacagc caatttgtta tgttaaacca attccacaaa
     8401 cttgcccatt tatctaattc caataattct tgttcattct tttcttgctg gttttgcgat
     8461 tcttcaatta aggagtgtat taagcttgtg taattgttaa tttctctgtc ccactccatc
     8521 caggtcgtgt gattccaaat ctgttccaga gatttattac tccaactagc attccaaggc
     8581 acagcagtgg tgcaaatgag ttttccagag caaccccaaa tccccaggag ctgttgatcc
     8641 tttaggtatc tttccacagc caggattctt gcctggagct gcttgatgcc ccagactgtg
     8701 agttgcaaca gatgctgttg cgcctcaata gccctcagca aattgttctg ctgctgcact
     8761 ataccagaca ataattgtct ggcctgtacc gtcagcgtca ttgacgctgc gcccatagtg
     8821 cttcctgctg ctcccaagaa cccaaggaac aaagctccta ttcccactgc tcttttttct
     8881 ctctgcacca ctcttctctt tgccttggtg ggtgctactc ctaatggttc aatttttact
     8941 actttatatt tatataattc acttctccaa ttgtccctca tatctcctcc tccaggtctg
     9001 aagatcagcg gccgcttgct gtgcggtggt cttacttttg ttttgctctt cctctatctt
     9061 gtctaaagct tccttggtgt cttttatctc tatcctttga tgcacacaat agagggttgc
     9121 tactgtatta tataatgatc taagttcttc tgatcctgtc tgaagggatg gttgtagctg
     9181 tcccagtatt tgtctacagc cttctgatgt ttctaacagg ccaggattaa ctgcgaatcg
     9241 ttctagctcc ctgcttgccc atactatatg ttttaattta tattttttct ttccccctgg
     9301 ccttaaccga attttttccc atcgcgatct aattctcccc cgcttaatac tgacgctctc
     9361 gcacccatct ctctccttct agcctccgct agtcaaaatt tttggcgtac tcaccagtcg
     9421 ccgcccctcg cctcttgccg tgcgcgcttc agcaagccga gtcctgcgtc gagagagctc
     9481 ctctggtttc ccttt
//
