LOCUS       sequence-462781-        9795 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A CMV promoter driving expression of a murine dominant negative myc 
            tagged TCF4..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9795)
  AUTHORS   Cantu Gutierrez ME, Hill MC, Largoza G, Martin JF, Wythe JD
  TITLE     Defining the Transcriptional and Epigenetic Basis of Organotypic 
            Endothelial Diversity in the Developing and Adult Mouse
  JOURNAL   bioRxiv 2021.11.15.468651
REFERENCE   2  (bases 1 to 9795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.11.15.468651"
COMMENT     Sequence Label: JDW 1038 (pLenti6.2_CMV_MYC_TCF4_DN_P2A_H2A_mC...
FEATURES             Location/Qualifiers
     source          1..9795
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    164..397
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    890..1015
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1062..1242
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(1243..1469)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(1497..1517)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1497..1515)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1536..1552)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1536..1552)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1549..1571)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1560..1576
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1584..1590,1591..1608,1609..1614))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1629..1650
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1767..1784)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1938..2526)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2018..2037)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2697..3488,3489..3557))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3320..3339
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3558..3662)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3688..4143)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3825..3846)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4037..4056
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4270..4292
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4284..4301
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4285..4301
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4311..4330
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4311..4329
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      4350..4485
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4525..4646
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4536..4555)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4590..4609
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4718..4951
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(5038..5436)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(5455..5502)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(5516..6015)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5564..5583)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     5957..5980
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     CDS             complement(6048..6089)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    6142..6166
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(6184..6894)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6264..6287)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(6462..6481)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     6731..6749
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(7291..7347)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(8737..8761)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(8857..8881)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(8878..9081)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(8907..8927)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        9082..9385
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(9730..9774)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ttcaattaag gagtgtatta agcttgtgta attgttaatt tctctgtccc actccatcca
       61 ggtcgtgtga ttccaaatct gttccagaga tttattactc caactagcat tccaaggcac
      121 agcagtggtg caaatgagtt ttccagagca accccaaatc cccaggagct gttgatcctt
      181 taggtatctt tccacagcca ggattcttgc ctggagctgc ttgatgcccc agactgtgag
      241 ttgcaacaga tgctgttgcg cctcaatagc cctcagcaaa ttgttctgct gctgcactat
      301 accagacaat aattgtctgg cctgtaccgt cagcgtcatt gacgctgcgc ccatagtgct
      361 tcctgctgct cccaagaacc caaggaacaa agctcctatt cccactgctc ttttttctct
      421 ctgcaccact cttctctttg ccttggtggg tgctactcct aatggttcaa tttttactac
      481 tttatattta tataattcac ttctccaatt gtccctcata tctcctcctc caggtctgaa
      541 gatcagcggc cgcttgctgt gcggtggtct tacttttgtt ttgctcttcc tctatcttgt
      601 ctaaagcttc cttggtgtct tttatctcta tcctttgatg cacacaatag agggttgcta
      661 ctgtattata taatgatcta agttcttctg atcctgtctg aagggatggt tgtagctgtc
      721 ccagtatttg tctacagcct tctgatgttt ctaacaggcc aggattaact gcgaatcgtt
      781 ctagctccct gcttgcccat actatatgtt ttaatttata ttttttcttt ccccctggcc
      841 ttaaccgaat tttttcccat cgcgatctaa ttctcccccg cttaatactg acgctctcgc
      901 acccatctct ctccttctag cctccgctag tcaaaatttt tggcgtactc accagtcgcc
      961 gcccctcgcc tcttgccgtg cgcgcttcag caagccgagt cctgcgtcga gagagctcct
     1021 ctggtttccc tttcgctttc aagtccctgt tcgggcgcca ctgctagaga ttttccacac
     1081 tgactaaaag ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac
     1141 tacttgaagc actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc
     1201 agagagctcc caggctcaga tctggtctaa ccagagagac ccgtttatgt atcgagctag
     1261 gcacttaaat acaatatctc tgcaatgcgg caattcagtg gttcgtccaa tccatgtcag
     1321 acccgtctgt tgccttccta ataaggcacg atcgtaccac cttacttcca ccaatcggca
     1381 tgcacggtgc tttttctctc cttgtaaggc atgttgctaa ctcatcgtta ccatgttgca
     1441 agactacaag agtattgcat aagactacat taagcttgca gctccagctt ttgttccctt
     1501 tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     1561 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     1621 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     1681 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     1741 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     1801 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     1861 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     1921 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     1981 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     2041 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     2101 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     2161 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     2221 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     2281 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     2341 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     2401 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     2461 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     2521 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     2581 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     2641 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     2701 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     2761 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     2821 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     2881 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     2941 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     3001 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     3061 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     3121 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     3181 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     3241 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     3301 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     3361 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     3421 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     3481 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     3541 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     3601 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     3661 cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg
     3721 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     3781 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     3841 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     3901 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     3961 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     4021 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     4081 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     4141 cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     4201 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     4261 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact
     4321 cactataggg cgaattgggt acgtccctag gcctccaaaa aagcctcctc actacttctg
     4381 gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc
     4441 atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg
     4501 cgggatagct agagccagac atgataagat acattgatga gtttggacaa accacaacta
     4561 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     4621 ccattataag ctgcaataaa caagttcctc tcactctctg atattcattt ctttgcaagt
     4681 tataaatact gaataataag atgacatgaa ctactactgc tagagatttt ccacactgac
     4741 taaaagggtc tgagggatct ctagttacca gagtcacaca acagacgggc acacactact
     4801 tgaagcactc aaggcaagct ttattgaggc ttaagcagtg ggttccctag ttagccagag
     4861 agctcccagg ctcagatctg gtctaaccag agagacccag tacaagcaaa aagcagatct
     4921 tgtcttcgtt gggagtgaat tagcccttcc agtcccccct tttcttttaa aaagtggcta
     4981 agatctacag ctgccttgta agtcattggt cttaaaggta ccgagctcga attgtgctta
     5041 gccctcccac acataaccag agggcagcaa ttcacgaatc ccaactgccg tcggctgtcc
     5101 atcactgtcc ttcactatgg ctttgatccc aggatgcaga tcgagaagca cctgtcggca
     5161 ccgtccgcag gggctcaaga tgcccctgtt ctcatttccg atcgcgacga tacaagtcag
     5221 gttgccagct gccgcagcag cagcagtgcc cagcaccacg agttctgcac aaggtccccc
     5281 agtaaaatga tatacattga caccagtgaa gatgcggccg tcgctagaga gagctgcgct
     5341 ggcgacgctg tagtcttcag agatggggat gctgttgatt gtagccgttg ctctttcaat
     5401 gagggtggat tcttcttgag acaaaggctt ggccatggtt tagttcctca ccttgtcgta
     5461 ttatactatg ccgatatact atgccgatga ttaattgtca acacgtgtct agagtcgaaa
     5521 ggcccggaga tgaggaagag gagaacagcg cggcagacgt gcgcttttga agcgtgcaga
     5581 atgccgggcc tccggaggac cttcgggcgc ccgccccgcc cctgagcccg cccctgagcc
     5641 cgcccccgga cccacccctt cccagcctct gagcccagaa agcgaaggag caaagctgct
     5701 attggccgct gccccaaagg cctacccgct tccattgctc agcggtgctg tccatctgca
     5761 cgagactagt gagacgtgct acttccattt gtcacgtcct gcacgacgcg agctgcgggg
     5821 cgggggggaa cttcctgact aggggaggag tagaaggtgg cgcgaagggg ccaccaaaga
     5881 acggagccgg ttggcgccta ccggtggatg tggaatgtgt gcgaggccag aggccacttg
     5941 tgtagcgcca agtgcccagc ggggctgcta aagcgcatgc tccagactgc cttgggaaaa
     6001 gcgcctcccc tacccggtag gtaccatcgt cattactaac cggtacgcgt agaatcgaga
     6061 ccgaggagag ggttagggat aggcttacct tcgaaccgcg ggccctctag actcgagcgg
     6121 ccgccactgt gctggatatc aaccactttg tacaagaaag ctgggagatc tctcgagcta
     6181 tcattacttg tacagctcgt ccatgccgcc ggtggagtgg cggccctcgg cgcgctcgta
     6241 ctgttccacg atggtgtagt cctcgttgtg ggaggtgatg tccagcttga tgttgacgtt
     6301 gtaggcgccg ggcagctgca cgggcttctt ggccttgtag gtggtcttga cctcggcgtc
     6361 gtagtggccg ccgtccttca gcttcagcct ctgcttgatc tcgcccttca gggcgccgtc
     6421 ctcggggtac atccgctcgg aggaggcctc ccagcccatg gtcttcttct gcattacggg
     6481 gccgtcggag gggaagttgg tgccgcgcag cttcaccttg tagatgaact cgccgtcctg
     6541 cagggaggag tcctgggtca cggtcaccac gccgccgtcc tcgaagttca tcacgcgctc
     6601 ccacttgaag ccctcgggga aggacagctt caagtagtcg gggatgtcgg cggggtgctt
     6661 cacgtaggcc ttggagccgt acatgaactg aggggacagg atgtcccagg cgaagggcag
     6721 ggggccgccc ttggtcacct tcagcttggc ggtctgggtg ccctcgtagg ggcggccctc
     6781 gccctcgccc tcgatctcga actcgtggcc gttcacggag ccctccatgt gcaccttgaa
     6841 gcgcatgaac tccttgatga tggccatgtt gtcctcctcg cccttgctca ccatcccccc
     6901 tgcggttttc tgctggccct tcttaccaat cagagacttg tggatgtgag gaatgactcc
     6961 tcctccagca atggtggcct tgataagcga gtccagctcc tcgtctccgc ggatggccag
     7021 ctgtaaatgt ctgggagtga ttcgcttcac cttcagatcc ttagaagcgt ttccagccaa
     7081 ctccagcacc tcagcggtga gatactccag gatagcagca ctgtaaaccg cagctgtagc
     7141 tccgacacgg ccatggctgg tggttcgtgt cttcaggtgc ctgtggatac gacctacagg
     7201 gaactgcaga ccagctctct gagatctgga gacggctttc gtcttggctt ttccactgtc
     7261 tttacctgct tttccacctg ccatcctagg tggcccggga ttctcttcga catcccctgc
     7321 ttgtttcaac agggagaagt tagtggctcc ggtacctcga agactgcagg ggccgcacca
     7381 gttattctgt tgatcaaggc caaagcgcgc tcggcatttc ttaggagcgc tcaggtctgt
     7441 gatcggagga agcgaaaggc aaggatttag gaaacattcg ctgtgttcgt tggtttcccc
     7501 cggctgcttg tctctttttc tcttcttctt cttcccatag ttatcccgtg cagaccagcc
     7561 agggtacagc tgcatgtgaa gctgtcgttc cttccgggcc agctcgtagt attttgcctg
     7621 ttcttccctg gacagggcgt gccacctgcg cccgagaatc tggttgatgg ctgcactctc
     7681 tttcaatgtg cattcggcca ccaccttcgc tctcatctct ttcatataca acatgaatgc
     7741 attaaggggc ttctttatgt ggggcttctt cttctcttct tcctttttgg agtcctgatg
     7801 ctttgagctg tggagtgagc cgacgtcact ctgggaggat tcctgcttga ctgtcggtgt
     7861 gacgatggcc gggtgaggga tgccggtcgt gtgcagagtg tgatggggag ggaccatatg
     7921 gggagggaac ctagacatag atgcgttgac tgtcagcgct gtggggtagg ggtgtctgaa
     7981 tcctcctgtc gtgattgggt acacaggctg accttgctgt ggtactaacc atcctagcgg
     8041 atgggggatt tgtcctacgg tgccgggcga cagcgggtaa tatggagaga tatctggagg
     8101 gtgcggaggc cttgggattc ctgttttggg gtctacgtca gctggtaagt gcggaggtgg
     8161 atttcccggg gtgaagtgtt cattgctgta cgtgatgaga ggcgtgagtg ggtggacatg
     8221 gtgggggtgt tgcaccaccg gtactttgtt cgaaacgatg tgtgctggcg acggcgaacg
     8281 agcatccttg agggtttgtc tgctctggag gcttcctgct tggacatcaa gcagtggcca
     8341 tttcatctga agatactgaa ttgcaatctg gtgttcaatc gttttgtacg cagagtaatg
     8401 agtgctgccg gattgaaaat ggagggttcg ggcggtgggc gagagcgatc cgttggggag
     8461 gtaggggctc gtcaggtcgg ggatcatgat gaaggggtag ccgggatacg gtggcccctt
     8521 aaagagccct ccatcttgcc tcttggccgc ttcttccaaa ctttcccggg atttatctcg
     8581 gaaactttcg gagcgaggcg gaggccgtct ttccgcctcg gaatcggagg agctgtcttg
     8641 catagagatc tcggtcgacc gaattcgggc ctccatggcc ataagcaggt cctcctctga
     8701 gatcagcttc tgcattgatg ccatggtggg ggatccagcc tgcttttttg tacaaacttg
     8761 ttgatatctg cagaattcca ccacactgga ctagtggatc ctctagagtc ggtgtcttct
     8821 atggaggtca aaacagcgtg gatggcgtct ccaggcgatc tgacggttca ctaaacgagc
     8881 tctgcttata tagacctccc accgtacacg cctaccgccc atttgcgtca atggggcgga
     8941 gttgttacga cattttggaa agtcccgttg attttggtgc caaaacaaac tcccattgac
     9001 gtcaatgggg tggagacttg gaaatccccg tgagtcaaac cgctatccac gcccattgat
     9061 gtactgccaa aaccgcatca ccatggtaat agcgatgact aatacgtaga tgtactgcca
     9121 agtaggaaag tcccataagg tcatgtactg ggcataatgc caggcgggcc atttaccgtc
     9181 attgacgtca atagggggcg tacttggcat atgatacact tgatgtactg ccaagtgggc
     9241 agtttaccgt aaatactcca cccattgacg tcaatggaaa gtccctattg gcgttactat
     9301 gggaacatac gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg
     9361 gccatttacc gtaagttatg taacgcggaa ctcccaagct tatcgcagac ccagtcgcta
     9421 gcgttaacgt cgacatcgca gacccagtcg ctagcgttaa cgtcgacatc gataccgtcg
     9481 agatccgttc actaatcgaa tggatctgtc tctgtctctc tctccacctt cttcttctat
     9541 tccttcgggc ctgtcgggtc ccctcggggt tgggaggtgg gtctgaaacg ataatggtga
     9601 atatccctgc ctaactctat tcactataga aagtacagca aaaactattc ttaaacctac
     9661 caagcctcct actatcatta tgaataattt tatataccac agccaatttg ttatgttaaa
     9721 ccaattccac aaacttgccc atttatctaa ttccaataat tcttgttcat tcttttcttg
     9781 ctggttttgc gattc
//
