LOCUS       sequence-462815-        4837 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MPS1 from Candida albicans codon optimized for S. cerevisiae in 
            pUCIDT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4837)
  AUTHORS   Fabritius A, Alonso A, Wood A, Sulthana S, Winey M
  TITLE     Spindle checkpoint activation by fungal orthologs of the S. 
            cerevisiae Mps1 kinase.
  JOURNAL   PLoS One. 2024 Mar 26;19(3):e0301084. doi: 
            10.1371/journal.pone.0301084. eCollection 2024.
  PUBMED    38530809
REFERENCE   2  (bases 1 to 4837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2024-03-26"; volume: "19(3):e0301084. doi"; pages: " 
            10.1371/journal.pone.0301084. eCollection 2024"
COMMENT     Sequence Label: MPS1 Candida albicans
FEATURES             Location/Qualifiers
     source          1..4837
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2616..2632)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2616..2632)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2629..2651)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2640..2656
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2664..2670,2671..2688,2689..2694))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2709..2730
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2847..2864)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3018..3606)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3098..3117)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3777..4568,4569..4637))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4400..4419
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4638..4742)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4810..4828
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accaaatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcatcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgcaac gcgatgacga tggatagcga
      421 ttcatcgatg agctgacccg atcgccgccg ccggagggtt gcgtttgaga cgggcgacag
      481 atctcgagat gccccacgat cttctaagcc atgtagctac gtctttctcc agtcaccgta
      541 gctcaccaag ctccaacaat tccacgaaga ggaggctagc ccttgaagat gaccccacag
      601 tgttgcctcc tgcgttaagc tcctacagta tacaatgctt aaacgagcgt accaagacct
      661 ctacgctgcc gagtcagatc acaggtaagt acgagttgga ctttcccgac agttcttcta
      721 agcgtgacaa tactttacgt aataagctgg cagcacactt cactaacgaa tccacgcgtt
      781 caagggaaat aagcaacagc ttcactaatc ttagcgagga ccgttcatct atcaacacag
      841 ctgccaccac tacccctggg tcaaaaacat tcgaggactt gcgttcaaaa agaaaaagga
      901 ggttcggtaa gtcactaggg ccaccaaagc gtggaaccga cttcgtacag gaatctacac
      961 ccataggaga cgagttatct cctaagatag agagcaagtt taacattacc ccacagcata
     1021 agagtttaag tgagattatc cctaaagctc cctctttgga gcgtttctcc ccactgctga
     1081 aaaagaatca cagcagcatc gacgccgagg ataccataac taagcgtatt gaggcaagga
     1141 ggcgtgaaca gatggagcag gagtttgtga agaaagaaaa ggagaatgaa cttgcggagt
     1201 taaaaataaa gaaattggag caaatcgcgg ccctgcagtc accttacgaa gagcacaaat
     1261 caaaagaatt tcaagagcca aaacaaagtc gtatgccgct aagagaaatt cccgtcaact
     1321 ctgcagtgga tgtcttcagg aaacctaaag cccctaagag tgccattagt ccgtcaccca
     1381 acgtccagtc aaatagcatg ataccaccag cacagcctgt ttttcgtaat acactacagg
     1441 attttccgaa accacctcag caaccacaag cctcctcaca gaaccagcag caggtgctac
     1501 cgtctgctcc tatcccccca aaggcccact acgataggcc gcaacactcc ggaaataaac
     1561 gtgccttaat tataaacgac aagtcttatg agaagctaga gcttatcggt aagggcggat
     1621 catctaaggt ttatagggtt aggtcattaa ataataattg cgtatatgcg ctgaagaagg
     1681 tggagcttgg acagtttgag gatgtttccg gattcaaggg cgagatcgat ttgctgacta
     1741 agttaaaaag ttgtgagaga gtagtcacat tggttgacta cgcaaccaca gaatcaagcc
     1801 tatacttaat catggaaaag ggggatcttg atcttgctga ggtccttcaa tatagattga
     1861 agttagatgc ccctttagat ttaaattttg taaagtacca taccattgag atgttcaagt
     1921 gtataaaaga cgtccacgat gcgggaatcg tccattctga tctgaaacct gccaatttcc
     1981 tgtttgtgcg tggtatgctg aaaattattg acttcggaat tgctaacgcg gtacctgacc
     2041 atacaatcaa tgtatatagg gagaatcaga taggaacgcc aaattacatg gcccccgagg
     2101 ccatttgcga aagcaactac acttctgcaa gaatctggaa ggtaggaaaa ccttcagaca
     2161 tctggagcat aggctgcatc ttgtatcaat tcatatatgg caaagcgccc ttcgcaggtt
     2221 attccggcaa ccaaaagctg atggccataa caaatcctca catcaagatt tcatttccta
     2281 gtagcgggat cggcaacacg cccgtacccg tgtccgcgat tgaattaatg aaaaattgcc
     2341 tacataggga cccgaacgat agatggacca tcgaacaatg tctaacctgc gactttctat
     2401 ctcctaagat tgtgagcgaa aatttcatcc gtgaggttgt ccaccaatcc attaactacg
     2461 ggtacaatag ccgtatatct ggggacggga tgacttcaga ccgttatgac gcactggttg
     2521 atagcgtaat gaagcagatt cagaatttaa attatagtta gtctagaatc agttctggac
     2581 cagcgagctg tgctgcgact cgtggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     2641 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     2701 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     2761 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     2821 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     2881 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     2941 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     3001 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     3061 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     3121 ggaagctccc tcgtgcgctc tcctgttccg accctgtcgc ttaccggata cctgtccgcc
     3181 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     3241 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     3301 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     3361 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     3421 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     3481 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     3541 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     3601 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     3661 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     3721 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     3781 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     3841 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     3901 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     3961 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     4021 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     4081 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     4141 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     4201 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     4261 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     4321 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     4381 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     4441 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     4501 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     4561 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     4621 aaatgttgaa tactcatact ctaccttttt caatattatt gaagcattta tcagggttat
     4681 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     4741 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     4801 acctataaaa ataggcgtat cacgaggccc tttcgtc
//
