LOCUS       sequence-462816-        5056 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MPS1 from Schizosaccharomyces pombe codon optimized for S. 
            cerevisiae in pUCIDT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5056)
  AUTHORS   Fabritius A, Alonso A, Wood A, Sulthana S, Winey M
  TITLE     Spindle checkpoint activation by fungal orthologs of the S. 
            cerevisiae Mps1 kinase.
  JOURNAL   PLoS One. 2024 Mar 26;19(3):e0301084. doi: 
            10.1371/journal.pone.0301084. eCollection 2024.
  PUBMED    38530809
REFERENCE   2  (bases 1 to 5056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2024-03-26"; volume: "19(3):e0301084. doi"; pages: " 
            10.1371/journal.pone.0301084. eCollection 2024"
COMMENT     Sequence Label: MPS1 Schizosaccharomyces pombe
FEATURES             Location/Qualifiers
     source          1..5056
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(20..42)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     142..161
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     355..377
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     369..386
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     370..386
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2826..2842)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2826..2842)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2839..2861)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2850..2866
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2874..2880,2881..2898,2899..2904))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2919..2940
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3057..3074)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3228..3816)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3308..3327)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3987..4778,4779..4847))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4610..4629
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4848..4952)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5020..5038
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
       61 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
      121 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccaaatgc
      181 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat
      241 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcatcgcta ttacgccagc
      301 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
      361 cacgacgttg taaaacgacg gccagtgcaa cgcgatgacg atggatagcg attcatcgat
      421 gagctgaccc gatcgccgcc gccggagggt tgcgtttgag acgggcgaca gatctcgaga
      481 tgtccacaaa ttctttccac gactatgtgg accttaagag tcgtacaaac acgagacagt
      541 tctccgatga cgaagaattc acaaccccgc ctaagttatc caacttcggc tccgcccttc
      601 ttagtcacac tgagaaaaca tcagcttcag aaatactaag ttctcacaat aacgacaaga
      661 ttgctaatag gctagaggaa atggacagaa gttcatcaag atctcatccg cctccatcta
      721 tgggcaattt aaccagcgga cacacatcta cctctagtca cagtacacta tttggccgtt
      781 atcttagaaa taaccaccaa acttccatga cgacgatgaa cacttccgac attgagatca
      841 acgtaggtaa ctcacttgac aagagtttcg agcgtatcag gaacttgagg caaaacatga
      901 aggaagatat aacaagcaaa tacgccgaaa ggaggtctaa gcgttttcta ataagtaaca
      961 gaaccaccaa gttaggtccg gctaagcgtg ctatgacatt aactaacata ttcgacgagg
     1021 atgtaccaaa ttctccaaac cagcctataa acgcgaggga gacagttgag ttaccattag
     1081 aggattctca ccaaacgaat ttcaaggaaa cgaagcgtaa taccgactat gatagcattg
     1141 attttgggga tttaaatcca atacagtaca tcaaaaagca taacctgccc acatcagatt
     1201 tgccgttgat ctcccaaatt tattttgaca agcagagaga agagaacagg caggcagctc
     1261 tgaggaagca ctccagtcgt gagctacttt acaagagccg tagcagtagc agcagtttaa
     1321 gcagtaataa tttgctagcg aacaaagaca atagcattac ttctaacacg ggttcccagc
     1381 caaggcgtaa ggtgagtacc ggatcatctt cttccaagtc ttctattgaa atccgtagag
     1441 ccttgaaaga gaatatcgac accagtaata acagtaattt caattccccg atccataaaa
     1501 tctacaaagg aataagcaga aacaaagact ccgattccga aaaaagagaa gtgttaagaa
     1561 acattagtat caatgcaaat catgctgaca acttacttca acaagaaaac aagagattaa
     1621 agagatcatt ggacgacgca attacaaacg aaaacataaa tagtaagaac ctagaggtct
     1681 tctaccacag gcctgctcct aaaccgcctg tcaccaagaa ggtcgagatc gtagaacccg
     1741 ctaagagtgc ttcccttagt aacaaccgta atattatcac tgtaaacgat agtcagtacg
     1801 aaaaaattga actactagga cgtggcggga gctcaagagt ctataaggtt aagggtagtg
     1861 gaaacagggt ttatgctcta aagagggtct ccttcgacgc ctttgacgac tccagtatcg
     1921 atggttttaa gggggagatc gagctgcttg agaagcttaa agatcagaag agagttattc
     1981 aacttttaga ttatgaaatg ggcgacggtt tactttattt gataatggag tgcggagatc
     2041 atgaccttag tcagattttg aaccaaaggt caggaatgcc tctggacttc aacttcgtaa
     2101 ggttttacac caaagagatg ctactttgca ttaaagtagt acacgatgcg ggcatcgttc
     2161 actccgatct aaagcccgca aactttgtac ttgtgaaggg catcttgaaa attattgact
     2221 tcggcattgc gaatgctgta ccggaacaca ccgttaatat ataccgtgag acacagatcg
     2281 gcacgcctaa ctacatggct ccagaggcgc tggtagccat gaactataca cagaatagcg
     2341 aaaatcaaca tgaagggaac aagtggaaag tcgggaggcc tagcgacatg tggagctgtg
     2401 gttgcatcat ataccagatg atttacggca aacctccata cggctcattt caggggcaaa
     2461 ataggttgct tgcaatcatg aatccagacg tgaaaatacc attccctgaa cacacctcta
     2521 ataacgagaa aatcccaaag agcgccatcg aacttatgaa ggcctgccta tatagaaacc
     2581 cggataaacg ttggactgta gataaggtcc tttcctcaac gttcctacag cccttcatga
     2641 tttctggatc aataatggag gacttaattc gtaatgcggt aagatacggg agcgagaagc
     2701 ctcacatctc acaagacgat ctaaacgatg tggtggatac cgtactgcgt aagttcgcag
     2761 attacaagat ctctagaatc agttctggac cagcgagctg tgctgcgact cgtggcgtaa
     2821 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     2881 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     2941 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     3001 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     3061 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     3121 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     3181 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     3241 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     3301 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     3361 accctgtcgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     3421 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     3481 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     3541 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     3601 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     3661 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     3721 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     3781 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     3841 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     3901 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     3961 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     4021 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     4081 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     4141 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     4201 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     4261 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     4321 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     4381 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     4441 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     4501 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     4561 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     4621 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     4681 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     4741 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     4801 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact ctaccttttt
     4861 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     4921 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     4981 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     5041 tttcgtctcg cgcgtt
//
