LOCUS       sequence-462817-        5089 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MPS1 from Candida glabrata codon optimized for S. cerevisiae in 
            pUCIDT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5089)
  AUTHORS   Fabritius A, Alonso A, Wood A, Sulthana S, Winey M
  TITLE     Spindle checkpoint activation by fungal orthologs of the S. 
            cerevisiae Mps1 kinase.
  JOURNAL   PLoS One. 2024 Mar 26;19(3):e0301084. doi: 
            10.1371/journal.pone.0301084. eCollection 2024.
  PUBMED    38530809
REFERENCE   2  (bases 1 to 5089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2024-03-26"; volume: "19(3):e0301084. doi"; pages: " 
            10.1371/journal.pone.0301084. eCollection 2024"
COMMENT     Sequence Label: MPS1 Candida glabrata
FEATURES             Location/Qualifiers
     source          1..5089
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1..23)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     123..142
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     336..358
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     350..367
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     351..367
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2840..2856)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2840..2856)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2853..2875)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2864..2880
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2888..2894,2895..2912,2913..2918))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2933..2954
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3071..3088)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3242..3830)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3322..3341)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4001..4792,4793..4861))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4624..4643
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4862..4966)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5034..5052
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
       61 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
      121 tgcggcatca gagcagattg tactgagagt gcaccaaatg cggtgtgaaa taccgcacag
      181 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg
      241 ggaagggcga tcggtgcggg cctcatcgct attacgccag ctggcgaaag ggggatgtgc
      301 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
      361 ggccagtgca acgcgatgac gatggatagc gattcatcga tgagctgacc cgatcgccgc
      421 cgccggaggg ttgcgtttga gacgggcgac agattctaga atgggtaacc cctaccactc
      481 agaaagcgac cagatttgga ggatttcatc acaggacagc gatgacggtg aaatcgggaa
      541 ccctcctaag ttgagtaact tcggtagtga gctaattagt gacgccatcg aaaaaataaa
      601 cccgaacaga tcagataaga aattgatcag cgaggagact cgtatccacg aaagtcattc
      661 acagcaacta aatacggaca ttaatcagtc tacgagggtc actcacagtg catcaagccc
      721 actttacttt gaggatacct cacccagcac catgttcgtc caaagccaag ctagtgccca
      781 gccctctgcc acgacgtatg atatctctag ccaagactac agcgactcat ccaagtcatt
      841 tataaaattg aagtccaaac aagatacttt aaagcaggag ttgacaacgc gttatagcga
      901 gagaaggacc agaagattcc ttcatgccac caacaggacc actaaattag ggcccgcact
      961 tcgtaacctt acctcaccgg ctcttgccat caatgataac aattctattg acaataagac
     1021 caatgagacg tccaaagtta acagtagctt tgacactaac aatgcgtatg tagacttcca
     1081 tcagactaag caggatatct acacatctgg tgaggagcat aacaatacta aagataatgg
     1141 taagataggg gccacgcaac agaacgatta ctcagacata aatatccatg agtgtaatcc
     1201 catacagtat atgaaaatgc ataacctgcc cacttccgag ctatcaaaaa tatcaaaggc
     1261 atattttgaa aaacagaagg aagagaatcg taaaaaagcg ctaaagaatg tgaacatgaa
     1321 gcagagccta aactacgact tgaagtatta ttctaactca agcccctcta tcgaatctgg
     1381 aaaggaaaac aatctaattc acaacaccga aatagaagac tttaatggtc gtggacacag
     1441 cacgaataac cacagcagta gctatctgag taacagtcac tctcaccccg tctccactca
     1501 gtttgttgaa tctgcgaaca tcgaactaga gaaaccaacc ttgaaaaaga gcctttcaaa
     1561 cgctgattct tacaggcaca cacagaggga ggcgttacaa aacatcgacg ttaattctaa
     1621 tgtgcgtgac ggagcaatag attacaagag acagaaggtc gcactaaagg attcagagtt
     1681 tgatgggatc cttgacaaaa agaagtatcg tggcattaag cgtgtggaaa tcgtagagcc
     1741 acccacatca acaagaccac cgtcaagaaa gaacgtagtg tctgtgaatg gtactgagta
     1801 tgaaagagta gagctactag gtagaggagg ttcttcaaac gtatataagg tcaggggcct
     1861 aaaaaacagg gtctatgctc taaagaaagt gtccttcgat gaattcgacg agagtagtgt
     1921 agaaggcttc aaaggggaaa tctcactttt gaagcagcta caaaatcaga acagagtagt
     1981 ccagctatat gactacgaaa tgggcagcgg ggtgctttac ctgctaatgg aatgtggaga
     2041 ctatgatctg agccaagtgt tgcaccaacg tacgaaccaa cctttcgaca tggaattcat
     2101 acgttatcat gcgagagaga tggtaacctg cgtaaaagtg gtacatgacg ccggaatagt
     2161 acattcagac ctgaagccag cgaattttgt gttcgtgaag gggatcttga agatcataga
     2221 tttcggcata gcaaatgcgg tgcctgatca cacggttaac atctataggg acatgcaaat
     2281 tggtacacct aactacatgg ccccggaggc tttggtagcc aacaactaca cggctgataa
     2341 tgatggaaaa tatgatcaga aaaccaacaa atggaaaatc ggcaagcctg cagacatatg
     2401 gtcatgcggc tgcatcattt atcagatgat ctacggaaaa ccgccctacg ctaagtacca
     2461 aggtcaggaa aggctattga gtattatgga tccaaatgtg aagatcgaat acaaagaggt
     2521 gttacccgat aagaccgtta tcccaagctt ggcactagaa ttaatgcaat actgtttaat
     2581 gaggaatcca gaagagaggt ggtccgcttt acaaatcttg gacagcccct ttcttaaccc
     2641 tttaatggtt agtcaggact ttataaagaa tcttatatct actgccgttt cctatggagc
     2701 tagggaaggg gacgtgagtc ctagtaggtt ggaggcgctt acggatagtg ttttagctcg
     2761 tctagctgac ttcaggatgc actagctcga gatcagttct ggaccagcga gctgtgctgc
     2821 gactcgtggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     2881 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     2941 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     3001 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     3061 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     3121 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     3181 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     3241 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     3301 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     3361 gctctcctgt tccgaccctg tcgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     3421 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     3481 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     3541 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     3601 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     3661 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     3721 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     3781 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     3841 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     3901 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     3961 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     4021 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     4081 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     4141 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     4201 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     4261 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     4321 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     4381 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     4441 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     4501 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     4561 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     4621 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     4681 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     4741 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     4801 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     4861 tactctacct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     4921 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     4981 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     5041 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaa
//
