LOCUS       sequence-462819-        5047 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MPS1 from Candida auris codon optimized for S. cerevisiae in pUCIDT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5047)
  AUTHORS   Fabritius A, Alonso A, Wood A, Sulthana S, Winey M
  TITLE     Spindle checkpoint activation by fungal orthologs of the S. 
            cerevisiae Mps1 kinase.
  JOURNAL   PLoS One. 2024 Mar 26;19(3):e0301084. doi: 
            10.1371/journal.pone.0301084. eCollection 2024.
  PUBMED    38530809
REFERENCE   2  (bases 1 to 5047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2024-03-26"; volume: "19(3):e0301084. doi"; pages: " 
            10.1371/journal.pone.0301084. eCollection 2024"
COMMENT     Sequence Label: MPS1 Candida auris
FEATURES             Location/Qualifiers
     source          1..5047
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     111..130
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     324..346
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     338..355
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     339..355
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2786..2802)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2786..2802)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2799..2821)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2810..2826
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2834..2840,2841..2858,2859..2864))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2879..2900
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3017..3034)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3188..3776)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3268..3287)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3947..4738,4739..4807))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4570..4589
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4808..4912)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4980..4998
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(join(5036..5047,1..11))
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
       61 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
      121 gcagattgta ctgagagtgc accaaatgcg gtgtgaaata ccgcacagat gcgtaaggag
      181 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc
      241 ggtgcgggcc tcatcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
      301 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgcaac
      361 gcgatgacga tggatagcga ttcatcgatg agctgacccg atcgccgccg ccggagggtt
      421 gcgtttgaga cgggcgacag atctcgagat gagcctacag ctaagctcag aagccccgta
      481 cagtgttaaa aggcgtatgc aggacgacga caatactttc cttccaccgc cgttgtccgc
      541 ttacggcatc gccagtctta gcgacaacag gagcgaagac gacaataata agagcggact
      601 gaagacagtt gagatcgggg aaaaccaagc gctaagagct agtttgagaa atagtctttc
      661 tgtacatttc aaagacagct caagtaggga ctcatacagt aactataatg acactcacaa
      721 caagagctat gggagccaga gctttagcgc aaacgagaga tttaatacca gcgccgcgaa
      781 caactcatcc cacaacgtct catgcaataa ctattcaggg aacttaagtg gccactcctt
      841 cagctctacg aatatgcata cggaagaaaa cgatgacttg atggaaggat caaacgatcc
      901 ttccgcaaag tctagtcata cttcagctac cacgccaggt ggtagccagg ccacagcagc
      961 actatcaaga tcctcattca cagggttcac taaacgttcc agatttgcca ggaggtttgc
     1021 ctcacttggt cccccgaaaa gggcgagcga ggtaagccct agccagaagt ctggtagctc
     1081 tccagaagcc gaaagaaatg gcgatccgat gattttgtct aagccagagg cgagtcagga
     1141 gttgatgaat gcgtttaaga cacctccaag tctacatgcg ttgcacaata agtctggcag
     1201 ggcctccccg aagagggtaa tcagtaacga gagtacattt ttcaaaagtt tggagaagct
     1261 tcgtaataac tctggggaca ttagcagaat ctctccgcca ttgataagta ggaaaaatag
     1321 tccgcccccc tttgttaaac cggtgattaa cgatgctcct agcaaggcag caagtccctt
     1381 ccaagtattc gtggacaagc aaccttccga aagaccctcc tctcgtggac ccagtcatcg
     1441 tccgtggcgt caaccgctgg ccaaagtcag cccaagcaag ataaattctg aggccgaagc
     1501 tgacgtattt aggaagccta agattccgaa agtgagcagt attccgaatc cgaccccacc
     1561 acctccacgt agttcatttg tgacgccgca gcaaccgttg ccggcgaaat ttccacatta
     1621 tcaagtcggt aaccaaaata ttgaccgtgc accagattcc tccgcgtccg gcagttcagg
     1681 catggtggaa gatggtaagc gtaagaaaat aatccacgtc aacaactctc agtatgaaaa
     1741 attggaactg ataggcagag gaggcacgtc aaaggtctac aaggtgagaa gcttgacgtc
     1801 aaaaaaaacc tatgcaataa aaaaggtagc gttcgaccaa tttgacgaat cttgcgtaaa
     1861 aggctttaag ggcgagatag acctgctgag caaactgaga gatcagccca gggtagttca
     1921 gctgcacgat tatgccatca atgacgggac catttactta acgatggaat gcggggaact
     1981 ggacttagcg cacgtgcttc agcaaaggca agccatggcg accgctctgg accttagctt
     2041 tgtccgtttc catgcgattg aggtcttaaa atgcgtagaa gcagtgcacg aggcaggaat
     2101 tgtccactca gacttgaagc cagccaactt cctatttgtt agaggaatta tgaaaatcat
     2161 cgattttggg attgctaacg ctgtccccga tcatacagca aacatctata gggaatcaca
     2221 aatcgggacc cctaactaca tggctccgga ggcattagta gaggtgaatc atactcttaa
     2281 tatgagtcgt ggaagcgcgg ccaatacatg gaaggtcggt aagccgtcag acatatggtc
     2341 atgtggttgc atcatttacc agatgattta cggaagacct ccatacgcta gctatgccgg
     2401 gcaacaaagg atcatggcta ttatgaaccc ccaagtacag gtccactatc cagctaatgg
     2461 tttgggtgat gtacccgtcc cgaaatctgc catagagctg atgaaaaagt gccttgtgcg
     2521 taatccacac gagaggtgga cagtgcaaca gtgtctggcg tcagactttc taaaaccaaa
     2581 ggctgttagt actgatttca tcaaggatgt ggtaaggtct gcagtaaact atggatatca
     2641 tagacaaagt cagggccaag tgtccgaaga agtatacgat aaactggtcg atagcataat
     2701 acaccagata aaagacctaa actatgccta gtctagaatc agttctggac cagcgagctg
     2761 tgctgcgact cgtggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     2821 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     2881 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     2941 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     3001 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     3061 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     3121 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     3181 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     3241 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     3301 tcgtgcgctc tcctgttccg accctgtcgc ttaccggata cctgtccgcc tttctccctt
     3361 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     3421 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     3481 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     3541 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     3601 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     3661 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     3721 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     3781 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     3841 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     3901 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     3961 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     4021 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     4081 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     4141 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     4201 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     4261 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     4321 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     4381 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     4441 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     4501 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     4561 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     4621 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     4681 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     4741 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     4801 tactcatact ctaccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     4861 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     4921 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     4981 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
     5041 gacacat
//
