LOCUS       sequence-462822-        4420 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MPS1 from Candia parapsilosis codon optimized for S. cerevisiae in 
            pUCIDT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Fabritius A, Alonso A, Wood A, Sulthana S, Winey M
  TITLE     Spindle checkpoint activation by fungal orthologs of the S. 
            cerevisiae Mps1 kinase.
  JOURNAL   PLoS One. 2024 Mar 26;19(3):e0301084. doi: 
            10.1371/journal.pone.0301084. eCollection 2024.
  PUBMED    38530809
REFERENCE   2  (bases 1 to 4420)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2024-03-26"; volume: "19(3):e0301084. doi"; pages: " 
            10.1371/journal.pone.0301084. eCollection 2024"
COMMENT     Sequence Label: MPS1 Candia parapsilosis
FEATURES             Location/Qualifiers
     source          1..4420
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     115..134
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     328..350
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     342..359
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     343..359
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2163..2179)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2163..2179)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2176..2198)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2187..2203
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2211..2217,2218..2235,2236..2241))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2256..2277
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2394..2411)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2565..3153)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2645..2664)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3324..4115,4116..4184))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3947..3966
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4185..4289)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4357..4375
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(join(4413..4420,1..15))
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
       61 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
      121 cagagcagat tgtactgaga gtgcaccaaa tgcggtgtga aataccgcac agatgcgtaa
      181 ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc
      241 gatcggtgcg ggcctcatcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
      301 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
      361 caacgcgatg acgatggata gcgattcatc gatgagctga cccgatcgcc gccgccggag
      421 ggttgcgttt gagacgggcg acagatctcg agatgtatga tattagtaac ccttctaata
      481 ggacgatgat agacgatgac aatacatgcg ctccaccgcc atctctatcc agttatagca
      541 ttgaaatgtt gaactcaaag aggaacaccc ctgtaaccgg gatctaccac caggacttcc
      601 cagagcgtag cacggactta cgtaaaaagt tgattgcagt ctttggacac acaaccacca
      661 ccacagaaaa cagtctatcc gatggactaa tggaggacaa aagtggttcc acatccaaaa
      721 gcacgattac tggcactgaa aatagggtaa agagacgtag atatggcaag gttttgggtg
      781 ctccaaagcg tgcctctcaa aataatacgc tggaagacga ctacatagat gaagaattaa
      841 taaaaaagaa cgtagaggaa aggaatccag gcaaaagaca accgcttgga gatatatcaa
      901 acactttccg taagcctttg acacctaaga agggagatga ggtggctgcc atgccaacga
      961 tggccgcgtc cgtccaagca cgtcccccac aagcgtcccc cataagacca agcatggcac
     1021 ccccggttgc ccatcctgtt cctcctccgg ctcccgctca gcctactatt gtacctccta
     1081 gacccgtagc agcaccgagt gaacctcgta cgttcaccgt gaataataag caatacgaaa
     1141 aaatggaact tctgggcagg ggcggatcaa gcaaggtata cagaattaaa gccgctaacg
     1201 gccaccaatt cgcgttaaag aaagtgactc ttaaccaggt cgaagatatt agctccttca
     1261 aaggtgaaat tgagttatta cgtaggctga gaaactataa aagggtcgtg aaactgtacg
     1321 actcagaggt caccaagtct tcaatctatc tgattatgga aagaggagac gtggacctag
     1381 ctactctttt ccaaacccgt ttgaacatga atctgccact ggatttgcaa ttcgtacgtt
     1441 atcatatttc agagatgttc aagtgtgtta aggatgtaca tgacgccggc gttgttcact
     1501 cagacctaaa accagctaat tttctgatgg tccgtggagt tttgaagatc atagactttg
     1561 ggatcgctaa cgcggtgcct gaccatacgg ccaatatata cagggaaagt cagattggga
     1621 cccccaacta catggcgccc gaagctcttg aggaagcttc tatggccgag accaaacata
     1681 cgacctggaa agtgggaaga ccgtccgaca tttggtcatg cggctgtatc atgtatcagt
     1741 ttatctatgg aagacctccg tacgcatctc taatgggaac gaaaagaata cttgcgataa
     1801 tgaacccaca atataagatc tcttacccag agctaggcat agggggggtt gccgttcctc
     1861 aaagtgcagt caatttaatg aggcactgtc tagctaggga ccctgatgac cgttggaccg
     1921 tagagcaatg tttaaattcc gagttctttc acccaaaggt agttgacgag aactttatta
     1981 ctaaaattgt tcaccagagc gtgaatcttg gttataataa gaggataagt ggggaaggta
     2041 tcaccaccga ggcatatgat gcgatggtca acgatatcat tgatcagata cgtacgtaca
     2101 attgctagtc tagaatcagt tctggaccag cgagctgtgc tgcgactcgt ggcgtaatca
     2161 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     2221 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     2281 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     2341 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     2401 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     2461 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     2521 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     2581 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     2641 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     2701 ctgtcgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     2761 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     2821 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     2881 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     2941 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     3001 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     3061 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     3121 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     3181 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     3241 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     3301 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     3361 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     3421 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     3481 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     3541 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     3601 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     3661 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     3721 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     3781 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     3841 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     3901 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     3961 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     4021 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     4081 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     4141 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactcta cctttttcaa
     4201 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     4261 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     4321 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     4381 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac
//
