LOCUS       sequence-462826-        5532 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AiE2118m_3xC1 is an enhancer sequence, designed to drive 
            AAV-mediated transgene expression in specific populations of brain 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Ben-Simon Y, Hooper M, Narayan S, Daigle TL, Dwivedi D, Way SW, 
            Oster A, Stafford DA, Mich JK, Taormina MJ, Martinez RA, Opitz-Araya
            X, Roth JR, Alexander JR, Allen S, Amster A, Arbuckle J, Ayala A, 
            Baker PM, Bakken TE, Barcelli T, Barta S, Bendrick J, Bertagnolli D,
            Bielstein C, Bishwakarma P, Bowlus J, Boyer G, Brouner K, Casian B, 
            Casper T, Chakka AB, Chakrabarty R, Chance RK, Chavan S, Clark M, 
            Colbert K, Collman F, Daniel S, Departee M, DiValentin P, Donadio N,
            Dotson N, Egdorf T, Fliss T, Gabitto M, Garcia J, Gary A, Gasperini 
            M, Gloe J, Goldy J, Gore BB, Graybuck L, Greisman N, Haeseleer F, 
            Halterman C, Haradon Z, Hastings SD, Helback O, Ho W, Hockemeyer D, 
            Huang C, Huff S, Hunker A, Johansen N, Jones D, Juneau Z, Kalmbach 
            B, Kannan M, Khem S, Kussick E, Kutsal R, Larsen R, Lee C, Lee AY, 
            Leibly M, Lenz GH, Li S, Liang E, Lusk N, Madigan Z, Malloy J, 
            Malone J, McCue R, Melchor J, Mollenkopf T, Moosman S, Morin E, 
            Newman D, Ng L, Ngo K, Omstead V, Otto S, Oyama A, Pena N, Pham T, 
            Phillips E, Pom CA, Potekhina L, Ransford S, Ray PL, Rette D, 
            Reynoldson C, Rimorin C, Rocha D, Ruiz A, Sanchez REA, Sawyer L, 
            Sedeno-Cortes A, Sevigny JP, Shapovalova N, Shepard N, Shulga L, 
            Sigler AR, Siverts L, Soliman S, Somasundaram S, Staats B, Stewart 
            K, Szelenyi E, Tieu M, Trader C, Tran A, van Velthoven CTJ, Walker 
            M, Wang Y, Weed N, Wirthlin M, Wood T, Wynalda B, Yao Z, Zhou T, 
            Ariza J, Dee N, Reding M, Ronellenfitch K, Mufti S, Sunkin SM, Smith
            KA, Esposito L, Waters J, Thyagarajan B, Yao S, Lein ES, Zeng H, 
            Levi BP, Ngai J, Ting JT, Tasic B
  TITLE     A suite of enhancer AAVs and transgenic mouse lines for genetic 
            access to cortical cell types.
  JOURNAL   Cell. 2025 May 16:S0092-8674(25)00513-6. doi: 
            10.1016/j.cell.2025.05.002.
  PUBMED    40403729
REFERENCE   2  (bases 1 to 5532)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            May 16:S0092-8674(25)00513-6. doi: 10.1016/j.cell.2025.05.002."
COMMENT     Sequence Label: AiP15380 -
            pAAV-AiE2118m_3xC1-minBG-iCre(R297T...
FEATURES             Location/Qualifiers
     source          1..5532
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      1468..1477
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1480..1500
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2566..2677
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2795..2935
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2795..2924
                     /label=AAV2 ITR
     rep_origin      3010..3465
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3097..3116)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3307..3328
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3482..3501)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3601..3623
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3661..3679)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3747..3851
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3852..3920,3921..4712)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4070..4089)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4883..5471
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5372..5391
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtcacttg caggtttctt cttccagtcc cagccctctg
      181 gcccacaccc cactgctgac cacaggagcc tctcacagtg ctcactcagg gcttcaaaac
      241 tctctccctg gcagcttctt tctattctga agtctcccag tccagcacaa attagtcccc
      301 tctcctggac tcctactgcg ccgccttggt acatcacaga gcactggctg agaaaatcgc
      361 cctttagtcc ccgcaaatca ctctcttacc tgctccactg actaggcctg tgccattatt
      421 tattagggaa tgagcgctag ctgttgaggg tcaattattc cattgaccag gcattacaat
      481 aattcctggt aattaaaaga ggtacttatg tgaatctgga tgcgtattga aagaaacatt
      541 agtccttttg tcagcttgca cttgcaggtt tcttcttcca gtcccagccc tctggcccac
      601 accccactgc tgaccacagg agcctctcac agtgctcact cagggcttca aaactctctc
      661 cctggcagct tctttctatt ctgaagtctc ccagtccagc acaaattagt cccctctcct
      721 ggactcctac tgcgccgcct tggtacatca cagagcactg gctgagaaaa tcgcccttta
      781 gtccccgcaa atcactctct tacctgctcc actgactagg cctgtgccat tatttattag
      841 ggaatgagcg ctagctgttg agggtcaatt attccattga ccaggcatta caataattcc
      901 tggtaattaa aagaggtact tatgtgaatc tggatgcgta ttgaaagaaa cattagtcct
      961 tttgtcagct tgcacttgca ggtttcttct tccagtccca gccctctggc ccacacccca
     1021 ctgctgacca caggagcctc tcacagtgct cactcagggc ttcaaaactc tctccctggc
     1081 agcttctttc tattctgaag tctcccagtc cagcacaaat tagtcccctc tcctggactc
     1141 ctactgcgcc gccttggtac atcacagagc actggctgag aaaatcgccc tttagtcccc
     1201 gcaaatcact ctcttacctg ctccactgac taggcctgtg ccattattta ttagggaatg
     1261 agcgctagct gttgagggtc aattattcca ttgaccaggc attacaataa ttcctggtaa
     1321 ttaaaagagg tacttatgtg aatctggatg cgtattgaaa gaaacattag tccttttgtc
     1381 agcttggagc tcgggctggg cataaaagtc agggcagagc catctattgc ttacatttgc
     1441 ttctgggatc cagatctttc gaagctagcc accatggtgc ccaagaagaa gaggaaagtc
     1501 tccaacctgc tgactgtgca ccaaaacctg cctgccctcc ctgtggatgc cacctctgat
     1561 gaagtcagga agaacctgat ggacatgttc agggacaggc aggccttctc tgaacacacc
     1621 tggaagatgc tcctgtctgt gtgcagatcc tgggctgcct ggtgcaagct gaacaacagg
     1681 aaatggttcc ctgctgaacc tgaggatgtg agggactacc tcctgtacct gcaagccaga
     1741 ggcctggctg tgaagaccat ccaacagcac ctgggccagc tcaacatgct gcacaggaga
     1801 tctggcctgc ctcgcccttc tgactccaat gctgtgtccc tggtgatgag gagaatcaga
     1861 aaggagaatg tggatgctgg ggagagagcc aagcaggccc tggcctttga acgcactgac
     1921 tttgaccaag tcagatccct gatggagaac tctgacagat gccaggacat caggaacctg
     1981 gccttcctgg gcattgccta caacaccctg ctgcgcattg ccgaaattgc cagaatcaga
     2041 gtgaaggaca tctcccgcac cgatggtggg agaatgctga tccacattgg caggaccaag
     2101 accctggtgt ccacagctgg tgtggagaag gccctgtccc tgggggttac caagctggtg
     2161 gagagatgga tctctgtgtc tggtgtggct gatgacccca acaactacct gttctgccgg
     2221 gtcagaaaga atggtgtggc tgccccttct gccacctccc aactgtccac ccgggccctg
     2281 gaagggatct ttgaggccac ccaccgcctg atctatggtg ccaaggatga ctctgggcag
     2341 agatacctgg cctggtctgg ccactctgcc agagtgggtg ctgccaccga catggccagg
     2401 gctggtgtgt ccatccctga aatcatgcag gctggtggct ggaccaatgt gaacattgtg
     2461 atgaactaca tcagaaacct ggactctgag actggggcca tggtgaggct gctcgaagat
     2521 ggggactgag gcgcgccgaa ttcaagcttc tcgagagatc ttcgactgtg ccttctagtt
     2581 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     2641 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     2701 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     2761 ggcatgagat ctcacgtgcg gaccgagcgg ccgcaggaac ccctagtgat ggagttggcc
     2821 actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc
     2881 ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc
     2941 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc
     3001 aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     3061 gcgtgaccgc tacacttgcc agcgccttag cgcccgctcc tttcgctttc ttcccttcct
     3121 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     3181 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac
     3241 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     3301 ttaatagtgg actcttgttc caaactggaa caacactcaa ctctatctcg ggctattctt
     3361 ttgatttata agggattttg ccgatttcgg tctattggtt aaaaaatgag ctgatttaac
     3421 aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca
     3481 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     3541 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     3601 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     3661 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     3721 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     3781 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     3841 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     3901 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     3961 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     4021 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     4081 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     4141 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     4201 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     4261 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     4321 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     4381 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     4441 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     4501 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     4561 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     4621 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     4681 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     4741 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     4801 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     4861 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     4921 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     4981 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     5041 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     5101 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     5161 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     5221 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     5281 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     5341 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     5401 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     5461 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     5521 ttgctcacat gt
//
