LOCUS       sequence-462827-        4523 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AiE0675m is an enhancer sequence, designed to drive AAV-mediated 
            transgene expression in specific populations of brain cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Ben-Simon Y, Hooper M, Narayan S, Daigle TL, Dwivedi D, Way SW, 
            Oster A, Stafford DA, Mich JK, Taormina MJ, Martinez RA, Opitz-Araya
            X, Roth JR, Alexander JR, Allen S, Amster A, Arbuckle J, Ayala A, 
            Baker PM, Bakken TE, Barcelli T, Barta S, Bendrick J, Bertagnolli D,
            Bielstein C, Bishwakarma P, Bowlus J, Boyer G, Brouner K, Casian B, 
            Casper T, Chakka AB, Chakrabarty R, Chance RK, Chavan S, Clark M, 
            Colbert K, Collman F, Daniel S, Departee M, DiValentin P, Donadio N,
            Dotson N, Egdorf T, Fliss T, Gabitto M, Garcia J, Gary A, Gasperini 
            M, Gloe J, Goldy J, Gore BB, Graybuck L, Greisman N, Haeseleer F, 
            Halterman C, Haradon Z, Hastings SD, Helback O, Ho W, Hockemeyer D, 
            Huang C, Huff S, Hunker A, Johansen N, Jones D, Juneau Z, Kalmbach 
            B, Kannan M, Khem S, Kussick E, Kutsal R, Larsen R, Lee C, Lee AY, 
            Leibly M, Lenz GH, Li S, Liang E, Lusk N, Madigan Z, Malloy J, 
            Malone J, McCue R, Melchor J, Mollenkopf T, Moosman S, Morin E, 
            Newman D, Ng L, Ngo K, Omstead V, Otto S, Oyama A, Pena N, Pham T, 
            Phillips E, Pom CA, Potekhina L, Ransford S, Ray PL, Rette D, 
            Reynoldson C, Rimorin C, Rocha D, Ruiz A, Sanchez REA, Sawyer L, 
            Sedeno-Cortes A, Sevigny JP, Shapovalova N, Shepard N, Shulga L, 
            Sigler AR, Siverts L, Soliman S, Somasundaram S, Staats B, Stewart 
            K, Szelenyi E, Tieu M, Trader C, Tran A, van Velthoven CTJ, Walker 
            M, Wang Y, Weed N, Wirthlin M, Wood T, Wynalda B, Yao Z, Zhou T, 
            Ariza J, Dee N, Reding M, Ronellenfitch K, Mufti S, Sunkin SM, Smith
            KA, Esposito L, Waters J, Thyagarajan B, Yao S, Lein ES, Zeng H, 
            Levi BP, Ngai J, Ting JT, Tasic B
  TITLE     A suite of enhancer AAVs and transgenic mouse lines for genetic 
            access to cortical cell types.
  JOURNAL   Cell. 2025 May 16:S0092-8674(25)00513-6. doi: 
            10.1016/j.cell.2025.05.002.
  PUBMED    40403729
REFERENCE   2  (bases 1 to 4523)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            May 16:S0092-8674(25)00513-6. doi: 10.1016/j.cell.2025.05.002."
COMMENT     Sequence Label: AiP14924 - pAAV-AiE0675m-minBG-iCre(297T)-BGHpA
FEATURES             Location/Qualifiers
     source          1..4523
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      459..468
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             471..491
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1557..1668
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   1786..1926
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   1786..1915
                     /label=AAV2 ITR
     rep_origin      2001..2456
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2088..2107)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2298..2319
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2473..2492)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2592..2614
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2652..2670)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2738..2842
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2843..2911,2912..3703)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3061..3080)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3874..4462
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4363..4382
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtaagcag agcccctttt gtcagccttc tctcttactg
      181 ttgtggttgt catcacaacc ccctccctcc agtaaaatga agacacggat tctccttggt
      241 tactggggga ggtatccagg atacgccagg cctccagcac cagaataaga cagccaaggc
      301 ctccagcctt gtacactgaa gcagctcctt tcctctctag aacaaagcta gccattcctg
      361 aattacccag aacgcgtgag ctcgggctgg gcataaaagt cagggcagag ccatctattg
      421 cttacatttg cttctgggat ccagatcttt cgaagctagc caccatggtg cccaagaaga
      481 agaggaaagt ctccaacctg ctgactgtgc accaaaacct gcctgccctc cctgtggatg
      541 ccacctctga tgaagtcagg aagaacctga tggacatgtt cagggacagg caggccttct
      601 ctgaacacac ctggaagatg ctcctgtctg tgtgcagatc ctgggctgcc tggtgcaagc
      661 tgaacaacag gaaatggttc cctgctgaac ctgaggatgt gagggactac ctcctgtacc
      721 tgcaagccag aggcctggct gtgaagacca tccaacagca cctgggccag ctcaacatgc
      781 tgcacaggag atctggcctg cctcgccctt ctgactccaa tgctgtgtcc ctggtgatga
      841 ggagaatcag aaaggagaat gtggatgctg gggagagagc caagcaggcc ctggcctttg
      901 aacgcactga ctttgaccaa gtcagatccc tgatggagaa ctctgacaga tgccaggaca
      961 tcaggaacct ggccttcctg ggcattgcct acaacaccct gctgcgcatt gccgaaattg
     1021 ccagaatcag agtgaaggac atctcccgca ccgatggtgg gagaatgctg atccacattg
     1081 gcaggaccaa gaccctggtg tccacagctg gtgtggagaa ggccctgtcc ctgggggtta
     1141 ccaagctggt ggagagatgg atctctgtgt ctggtgtggc tgatgacccc aacaactacc
     1201 tgttctgccg ggtcagaaag aatggtgtgg ctgccccttc tgccacctcc caactgtcca
     1261 cccgggccct ggaagggatc tttgaggcca cccaccgcct gatctatggt gccaaggatg
     1321 actctgggca gagatacctg gcctggtctg gccactctgc cagagtgggt gctgccaccg
     1381 acatggccag ggctggtgtg tccatccctg aaatcatgca ggctggtggc tggaccaatg
     1441 tgaacattgt gatgaactac atcagaaacc tggactctga gactggggcc atggtgaggc
     1501 tgctcgaaga tggggactga ggcgcgccga attcaagctt ctcgagagat cttcgactgt
     1561 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     1621 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     1681 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     1741 agacaatagc aggcatgaga tctcacgtgc ggaccgagcg gccgcaggaa cccctagtga
     1801 tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg
     1861 tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc
     1921 tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     1981 tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     2041 ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt
     2101 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     2161 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg
     2221 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     2281 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca actctatctc
     2341 gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt taaaaaatga
     2401 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg
     2461 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     2521 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     2581 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     2641 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     2701 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     2761 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     2821 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     2881 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     2941 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     3001 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     3061 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     3121 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     3181 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     3241 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     3301 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     3361 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     3421 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     3481 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     3541 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     3601 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     3661 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     3721 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     3781 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     3841 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     3901 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     3961 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     4021 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     4081 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     4141 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     4201 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     4261 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     4321 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     4381 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     4441 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     4501 ttgctggcct tttgctcaca tgt
//
