LOCUS       sequence-46283-e         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Raj B, O'Hanlon D, Vessey JP, Pan Q, Ray D, Buckley NJ, Miller FD, 
            Blencowe BJ
  TITLE     Cross-regulation between an alternative splicing activator and a 
            transcription repressor controls neurogenesis.
  JOURNAL   Mol Cell. 2011 Sep 2;43(5):843-50.
  PUBMED    21884984
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2011-09-2"; volume: "43(5)"; pages: "843-50"
COMMENT     Sequence Label: pLDPuro-MmnSR100N
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(421..444)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(493..517)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(563..583)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 tttcttggag cttttcttct tcttcttctt gactggtgat gggctgtagg acggggacct
       61 cctcctcttt ttcctggttg atttcttctt tttctttttc cccctggagg aaggtggtgg
      121 tgtcgggtct ctgtcttgag aggcactgtg tgctctggca ggatccagtt ccctgcaggc
      181 cttctctctt ccccagctgt tggtacccag ggcctcaggg gccagatgtt ggcccagctt
      241 ttctgaaggt ccatcctgat ctgggagcaa gagactgctc tggggctctg tccttggcat
      301 cggcttgcgg gctgtgatac tggcgacgat gatgctctcg ggacgcggag tggccactgc
      361 tttgaaggtc cctttccaga acttctcaaa cagctgtttc tcgccctgct gcaggctagc
      421 cttgtcatcg tcatccttgt aatcgatgtc atgatcatat gtatcaccgt catggtcttt
      481 gtagtccatg gtagcctgct tttttgtaca aacttgtacg cgtggtacct tcacgacacc
      541 tgaaatggaa gaaaaaaact ttgaaccact gtctgaggct tgagaatgaa ccaagatcca
      601 aactcaaaaa gggcaaattc caaggagaat tacatcaagt gccaagctgg cctaacttca
      661 gtctccaccc actcagtgtg gggaaactcc atcgcataaa acccctcccc ccaacctaaa
      721 gacgacgtac tccaaaagct ccagaactaa tcgaggtgcc tggacggcgc ccggtactcc
      781 gtggagtcac atgaagcgac ggctgaggac
//
