LOCUS       sequence-462838-        9158 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Visualization of cAMP dynamics using YR-ICUE-mGold2t.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9158)
  AUTHORS   Lee J, Lai S, Yang S, Zhao S, Blanco FA, Lyons AC, Merino-Urteaga R,
            Ahrens JF, Nguyen NA, Liu H, Liu Z, Lambert GG, Shaner NC, Chen L, 
            Tolias KF, Zhang J, Ha T, St-Pierre F
  TITLE     Bright and photostable yellow fluorescent proteins for extended 
            imaging.
  JOURNAL   Nat Commun. 2025 Apr 4;16(1):3241. doi: 10.1038/s41467-025-58223-5.
  PUBMED    40185748
REFERENCE   2  (bases 1 to 9158)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-04-4"; volume: "16(1):3241. doi"; pages: " 
            10.1038/s41467-025-58223-5"
COMMENT     Sequence Label: pCMV-YR-ICUE-mGold2t
FEATURES             Location/Qualifiers
     source          1..9158
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     297..316
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(535..639)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(714..733)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        905..1284
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1285..1488
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1439..1459
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1533..1552
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1533..1551
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1571..1580
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1592..1609
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1613..1645
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(1649..1657,1658..1672)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             1679..2386
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1824..1842)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2092..2111
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             join(4598..4600,4601..4603,4604..5317)
                     /codon_start=1
                     /product="photostable derivative of mVenus (Lee et al., 
                     2020)"
                     /label=mGold
                     /note="mammalian codon-optimized"
                     /translation="MASKGEELFTGVVPILVELDGDINGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTSLGYGLQCFARYPDNMKRHDFFKSAMPEGYVQERTIFFEDD
                     GNYKTRAEVKFEGGTLVNRVELKGIDFKEDGNILGHKLEYNYNCHNVFITADRQQNGIK
                     VNFKIRHNIEVGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQTKLSKDPNEKRDHMVLL
                     EFVTAAGITLSMDELYK"
     primer_bind     complement(4904..4923)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5251..5272
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(5381..5399)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5382..5399)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5419..5436)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5425..5649
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5695..6123
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5782..5801)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5992..6013
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6132..6152)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6137..6466
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6317..6452
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6379..6398
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6533..7327
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6587..6606)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7197..7216
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7501..7622
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7538..7557)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7592..7611
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7671..7687)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7671..7687)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7684..7706)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7695..7711
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7719..7725,7726..7743,7744..7749))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7764..7785
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7902..7919)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8073..8661)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8153..8172)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8832..9158,1..465,466..534))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
       61 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
      121 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
      181 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
      241 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
      301 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
      361 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
      421 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
      481 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
      541 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
      601 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
      661 acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc
      721 tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct
      781 gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg
      841 aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg
      901 cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
      961 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     1021 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     1081 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     1141 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     1201 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     1261 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     1321 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     1381 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     1441 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact
     1501 agagaaccca ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa
     1561 gcttgcggcc gccaccatgg tgcggggttc tcatcatcat catcatcatg gtatggctag
     1621 catgactggt ggacagcaaa tgggtcggga tctgtacgac gatgacgata aggatcccat
     1681 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
     1741 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     1801 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
     1861 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
     1921 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     1981 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
     2041 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
     2101 gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
     2161 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
     2221 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
     2281 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
     2341 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagagat ctcccgtggg
     2401 aactcatgag atggaggagg agttggccga agctgtggcc ctgctctccc agcgggggcc
     2461 tgacgccctg ctcactgtgg cacttcgaaa gcccccaggt cagcgcacgg atgaagagct
     2521 ggacctcatc tttgaggagc tgctgcacat caaggctgtg gcccacctct ccaactcggt
     2581 gaagcgagaa ttagcggctg ttctgctctt tgaaccacac agcaaggcag ggaccgtgtt
     2641 gttcagccag ggggacaagg gcacttcgtg gtacattatc tggaagggat ctgtcaacgt
     2701 ggtgacccat ggcaaggggc tggtgaccac cctgcatgag ggagatgatt ttggacagct
     2761 ggctctggtg aatgatgcac cccgggcagc caccatcatc ctgcgagaag acaactgtca
     2821 tttcctgcgt gtggacaagc aggacttcaa ccgtatcatc aaggatgtgg aggcaaagac
     2881 catgcggctg gaagaacatg gcaaagtggt gctggtgctg gagagagcct ctcagggcgc
     2941 cggcccttcc cgacccccaa ccccaggcag gaaccggtat acagtgatgt ctggcacccc
     3001 agagaagatc ctagagcttc tgttggaggc catgggacca gattccagtg ctcatgacca
     3061 aacagagaca ttcctcagcg acttcctcct gacccacagg gtcttcatgc ccagcgccca
     3121 actctgcgct gcccttctgc accacttcca tgtggagcct gcgggtggca gcgagcagga
     3181 gcgcagcacc tacgtctgca acaagaggca gcagatcttg cggctggtca gccagtgggt
     3241 ggccctgtat ggctccatgc tccacactga ccctgtggcc accagcttcc tccagaaact
     3301 ctcagacctg gtgggcaggg acacccgact cagcaacctg ctgagggagc agtggccaga
     3361 gaggcggcga tgccacaggt tggagaatgg ctgtgggaat gcatctcctc agatgaaggc
     3421 ccggaacttg cctgtttggc tccccaacca ggacgagccc cttcctggca gcagctgtgc
     3481 catccaagtt ggggataaag tcccctatga catctgccgg ccagaccact cagtgttgac
     3541 cctgcagctg cctgtgacag cctccgtgag agaggtgatg gcagcgttgg cccaggagga
     3601 tggctggacc aaggggcagg tgctggtgaa ggtcaattct gcaggtgatg ccattggcct
     3661 gcagccagat gcccgtggtg tggccacatc tctggggctc aatgagcgtc tctttgttgt
     3721 caacccacag gaagcgcatg agctgatccc acaccctgac cagctggggc ccactgtggg
     3781 ctctgctgag gggctggacc tggtgagtgc caaggacctg gcaggccagc tgacggacca
     3841 cgactggagc ctcttcaaca gtatccacca ggtggagctg atccactatg tgctgggccc
     3901 ccagcatctg cgggatgtca ccaccgccaa cctggagcgc ttcatgcgcc gcttcaatga
     3961 gctgcagtac tgggtggcca ccgagctgtg tctctgcccc gtgcccggcc cccgggccca
     4021 gctgctcagg aagttcatta agctggcggc ccacctcaag gagcagaaga atctcaattc
     4081 cttctttgcc gtcatgtttg gcctcagcaa ctcggccatc agccacctag cccacacctg
     4141 ggagcggctg ccccacaaag tccggaagct gtactccgcc ctcgagaggc tgctggaccc
     4201 ctcatggaac caccgggtat accgactggc cctcgccaag ctctcccctc ctgtcatccc
     4261 cttcatgccc cttcttctca aagacatgac cttcattcat gagggaaacc acacactagt
     4321 ggagaatctc atcaactttg agaagatgag aatgatggcc agagtcgcgc ggatgctgca
     4381 ccactgccga agccacaacc ctgtgcctct ctcaccactc agaagccgag tttcccacct
     4441 ccacgaggac agccaggtgg cgaggatttc cacatgctcg gagcagtccc tgagcacccg
     4501 gagtccagcc agcacctggg cttatgtcca gcagctgaag gtcattgaca accagcggga
     4561 actctcccgc ctctcccgag agctggagcc aggtaccatg gcgagcaagg gcgaggagct
     4621 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacataaacg gccacaagtt
     4681 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     4741 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccagcc tgggctacgg
     4801 cctgcagtgc ttcgcccgct accccgacaa catgaagcgg cacgacttct tcaagtccgc
     4861 catgcccgaa ggctacgtcc aggagcgcac catcttcttc gaggacgacg gcaactacaa
     4921 gacccgcgcc gaggtgaagt ttgagggcgg caccctggtg aaccgcgtcg agctgaaggg
     4981 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaactg
     5041 ccacaacgtc tttatcaccg ccgacaggca gcagaacggc atcaaggtca acttcaagat
     5101 ccgccacaac atcgaggtcg gcggcgtgca gctcgccgac cactaccagc agaacacccc
     5161 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc agaccaagct
     5221 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     5281 cgggatcact ctcagcatgg acgagctgta caagtaagaa ttcaagaaga agaagaagag
     5341 caagaccaag tgcgtgatca tgtaatctag agggccctat tctatagtgt cacctaaatg
     5401 ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     5461 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     5521 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     5581 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     5641 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
     5701 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     5761 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     5821 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     5881 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     5941 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     6001 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     6061 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     6121 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     6181 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     6241 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     6301 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     6361 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     6421 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     6481 ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga
     6541 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     6601 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     6661 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
     6721 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     6781 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     6841 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     6901 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     6961 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     7021 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
     7081 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     7141 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     7201 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     7261 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     7321 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
     7381 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     7441 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
     7501 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     7561 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     7621 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     7681 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     7741 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     7801 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     7861 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     7921 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     7981 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     8041 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     8101 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     8161 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     8221 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     8281 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     8341 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     8401 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     8461 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     8521 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     8581 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     8641 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     8701 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     8761 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     8821 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     8881 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     8941 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     9001 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     9061 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     9121 agtttgcgca acgttgttgc cattgctaca ggcatcgt
//
