LOCUS       sequence-462878-       11518 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11518)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11518)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0457
FEATURES             Location/Qualifiers
     source          1..11518
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    7375..7963
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7428..7448)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7846..7857)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8035..8268
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8346..8467
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8383..8402)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8437..8456
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8507..8642
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8662..8681)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8663..8681)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8691..8708)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8691..8707)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8700..8722)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8849..9304
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8936..8955)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9146..9167
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9330..9434
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9435..9503,9504..10295)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9653..9672)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10466..11054
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10955..10974
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11208..11225
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11342..11363
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11378..11383,11384..11401,11402..11408)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11416..11432
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11421..11443
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11440..11456
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11440..11456
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11475..11495
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11477..11495
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggag gtggcggtgg agaaggcggt
     4141 ggcggcggcg gcagcggcct cggctgcggc ctccgggggg ccctcggcgg cgccgagcgg
     4201 ggagaacgag gccgagagtc ggcagggccc ggactcggag cgcggcggcg aggcggcccg
     4261 gctcaacctg ttggacactt gcgccgtgtg ccaccagaac atccagagcc gggcgcccaa
     4321 gctgctgccc tgcctgcact ctttctgcca gcgctgcctg cccgcgcccc agcgctacct
     4381 catgctgccc gcgcccatgc tgggctcggc cgagaccccg ccacccgtcc ctgcccccgg
     4441 ctcgccggtc agcggctcgt cgccgttcgc cacccaagtt ggagtcattc gttgcccagt
     4501 ttgcagccaa gaatgtgcag agagacacat catagataac ttttttgtga aggacactac
     4561 tgaggttccc agcagtacag tagaaaagtc aaatcaggta tgtacaagct gtgaggacaa
     4621 cgcagaagcc aatgggtttt gtgtagagtg tgttgaatgg ctctgcaaga cgtgtatcag
     4681 agctcatcag agggtaaagt tcacaaaaga ccacactgtc agacagaaag aggaagtatc
     4741 tccagaggca gttggtgtca ccagccagcg accagtgttt tgtccttttc ataaaaagga
     4801 gcagctgaag ctgtactgtg agacatgtga caaactgaca tgtcgagact gtcagttgtt
     4861 agaacataaa gagcatagat accaatttat agaagaagct tttcagaatc agaaagtgat
     4921 catagataca ctaatcacca aactgatgga aaaaacaaaa tacataaaat tcacaggaaa
     4981 tcagatccaa aacagaatta ttgaagtaaa tcaaaatcaa aagcaggtgg aacaggatat
     5041 taaagttgct atatttacac tgatggtaga aataaataaa aaaggaaaag ctctactgca
     5101 tcagttagag agccttgcaa aggaccatcg catgaaactt atgcaacaac aacaggaagt
     5161 ggctggactc tctaaacaat tggagcatgt catgcatttt tctaaatggg cagtttccag
     5221 tggcagcagt acagcattac tttatagcaa acgactgatt acataccggt tacggcacct
     5281 ccttcgtgca aggtgtgatg catccccagt gaccaacaac accatccaat ttcactgtga
     5341 tcctagtttc tgggctcaaa atatcatcaa cttaggttct ttagtaatcg aggataaaga
     5401 gagccagcca caaatgccta agcagaatcc tgtcgtggaa cagaattcac agccaccaag
     5461 tggtttatca tcaaaccagt tatccaagtt cccaacacag atcagcctag ctcaattacg
     5521 gctccagcat atgcagcaac aggtaatggc tcagaggcaa caggtgcaac ggaggccagc
     5581 acctgtgggt ttaccaaacc ctagaatgca ggggcccatc cagcaacctt ccatctctca
     5641 tcagcaaccg cctccacgtt tgataaactt tcagaatcac agccccaaac ccaatggacc
     5701 agttcttcct cctcatcctc aacaactgag atatccacca aaccagaaca taccacgaca
     5761 agcaataaag ccaaaccccc tacagatggc tttcttggct caacaagcca taaaacagtg
     5821 gcagatcagc agtggacagg gaaccccatc aactaccaac agcacatcct ctactccttc
     5881 cagccccacg attactagtg cagcaggata tgatggaaag gcttttggtt cacctatgat
     5941 cgatttgagc tcaccagtgg gagggtctta taatcttccc tctcttccgg atattgactg
     6001 ttcaagtact attatgctgg acaatattgt gaggaaagat actaatatag atcatggcca
     6061 gccaagacca ccctcaaaca gaacggtcca gtcaccaaat tcatcagtgc catctccagg
     6121 ccttgcagga cctgttacta tgactagtgt acacccccca atacgttcac ctagtgcctc
     6181 cagcgttgga agccgaggaa gctctggctc ttccagcaaa ccagcaggag ctgactctac
     6241 acacaaagtc ccagtggtca tgctggagcc aattcgaata aaacaagaaa acagtggacc
     6301 accggaaaat tatgatttcc ctgttgttat agtgaagcaa gaatcagatg aagaatctag
     6361 gcctcaaaat gccaattatc caagaagcat actcacctcc ctgctcttaa atagcagtca
     6421 gagctctact tctgaggaga ctgtgctaag atcagatgcc cctgatagta caggagatca
     6481 acctggactt caccaggaca attcctcaaa tggaaagtct gaatggttgg atccttccca
     6541 gaagtcacct cttcatgttg gagagacaag gaaagaggat gaccccaatg aggactggtg
     6601 tgcagtttgt caaaacggag gggaactcct ctgctgtgaa aagtgcccca aagtattcca
     6661 tctttcttgt catgtgccca cattgacaaa ttttccaagt ggagagtgga tttgcacttt
     6721 ctgccgagac ttatctaaac cagaagttga atatgattgt gatgctccca gtcacaactc
     6781 agaaaaaaag aaaactgaag gccttgttaa gttaacacct atagataaaa ggaagtgtga
     6841 gcgcctactt ttatttcttt actgccatga aatgagcctg gcttttcaag accctgttcc
     6901 tctaactgtg cctgattatt acaaaataat taaaaatcca atggatttgt caaccatcaa
     6961 gaaaagacta caagaagatt attccatgta ctcaaaacct gaagattttg tagctgattt
     7021 tagattgatc tttcaaaact gtgctgaatt caatgagcct gattcagaag tagccaatgc
     7081 tggtataaaa cttgaaaatt attttgaaga acttctaaag aacctctatc cagaaaaaag
     7141 gtttcccaaa ccagaattca ggaatgaatc agaagataat aaatttagtg atgattcaga
     7201 tgatgacttt gtacagcccc ggaagaaacg cctcaaaagc attgaagaac gccagttgct
     7261 taaatagtaa tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc
     7321 ttgtggaaag gacgatttgc ttcgccagaa ctgaacgaca cgcgttaagt cgacaatcaa
     7381 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     7441 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     7501 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     7561 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     7621 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     7681 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     7741 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     7801 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     7861 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     7921 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa
     7981 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     8041 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     8101 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     8161 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     8221 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt acgtatagta
     8281 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     8341 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     8401 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     8461 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     8521 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     8581 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     8641 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     8701 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     8761 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     8821 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     8881 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     8941 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     9001 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     9061 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     9121 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     9181 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     9241 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     9301 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     9361 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     9421 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     9481 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     9541 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     9601 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     9661 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     9721 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     9781 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     9841 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     9901 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     9961 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
    10021 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
    10081 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
    10141 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
    10201 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
    10261 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
    10321 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
    10381 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
    10441 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
    10501 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
    10561 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
    10621 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
    10681 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
    10741 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
    10801 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
    10861 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
    10921 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
    10981 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
    11041 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
    11101 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
    11161 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
    11221 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
    11281 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
    11341 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
    11401 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
    11461 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagc
//
