LOCUS       sequence-462884-       11017 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CROPseq vector enabling gRNA detection via mRNA-based capture 
            (CROPseq), 3'-based direct capture using 10x capture sequence 1 or 
            5'-based enrichment using a custom reverse transcription primer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11017)
  AUTHORS   Bock C
  TITLE     Integrated time series analysis and high-content CRISPR screening 
            delineates the dynamics of macrophage immune regulation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: CROPseq-3P5P
FEATURES             Location/Qualifiers
     source          1..11017
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      426..1014
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     915..934
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1168..1185
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1302..1323
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1338..1343,1344..1361,1362..1368)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1376..1392
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1381..1403
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1400..1416
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1400..1416
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1435..1455
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1437..1455
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1483..1709
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1710..1890
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1937..2062
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2555..2788
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2973..3017
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3198..3315
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3454..4632
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3685..4623
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4580..4600
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4645..5241
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4645..4664)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5141..5161
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             5251..5307
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(5453..5471)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     misc_feature    6034..6622
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6087..6107)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6505..6516)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6764..7012
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     6764..6784
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     6935..6954
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     LTR             9065..9245
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    9323..9444
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9360..9379)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9414..9433
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9484..9619
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9639..9658)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9640..9658)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9668..9685)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9668..9684)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9677..9699)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9826..10281
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9913..9932)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10123..10144
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10307..10411
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10412..10480,10481..11017,1..255)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10630..10649)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
       61 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
      121 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
      181 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
      241 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
      301 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
      361 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
      421 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
      481 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
      541 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
      601 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
      661 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
      721 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
      781 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
      841 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
      901 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
      961 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     1021 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     1081 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     1141 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     1201 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     1261 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     1321 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     1381 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
     1441 aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac
     1501 tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa
     1561 agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc
     1621 aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg
     1681 tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct
     1741 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     1801 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     1861 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
     1921 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
     1981 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
     2041 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
     2101 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     2161 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     2221 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     2281 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     2341 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     2401 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     2461 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     2521 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     2581 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     2641 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     2701 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     2761 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     2821 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     2881 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     2941 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     3001 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     3061 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     3121 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagaatctt gagacaaatg
     3181 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     3241 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     3301 aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg gcgccggctc
     3361 gagggggccc gggtgcaaag atggataaag ttttaaacag agaggaatct ttgcagctaa
     3421 tggaccttct aggtcttgaa aggagtggga attggctccg gtgcccgtca gtgggcagag
     3481 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg atccggtgcc
     3541 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     3601 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     3661 aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc
     3721 tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca gtacgtgatt
     3781 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga
     3841 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga
     3901 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat
     3961 ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa
     4021 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc
     4081 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg
     4141 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg
     4201 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt
     4261 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt
     4321 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc
     4381 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg
     4441 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag
     4501 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag
     4561 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt
     4621 caggtgtcgt gacgtacggc caccatgacc gagtacaagc ccacggtgcg cctcgccacc
     4681 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     4741 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     4801 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcc
     4861 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     4921 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     4981 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg agtctcgccc
     5041 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     5101 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     5161 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     5221 atgacccgca agcccggtgc cggatccgga gccacgaact tctctctgtt aaagcaagca
     5281 ggcgacgtgg aagaaaaccc cggtcctatg gtgagcaagg gcgaggagga caacatggcc
     5341 atcatcaagg agttcatgag gttcaaggtg cacatggagg gcagcgtgaa cggccacgag
     5401 ttcgagatcg agggcgaggg cgagggcagg ccctacgagg gcacccagac cgccaagctg
     5461 aaggtgacca agggcggccc cctgcccttc gcctgggaca tcctgagccc ccagttcatg
     5521 tacggcagca aggcctacgt gaagcacccc gccgacatcc ccgactacct gaagctgagc
     5581 ttccccgagg gcttcaactg ggagagggtg atgaacttcg aggacggcgg cgtggtgacc
     5641 gtgacccagg acagcagcct gcaggacggc gagttcatct acaaggtgaa gctgaggggc
     5701 accaacttcc ccagcgacgg ccccgtgatg cagtgcagga ccatgggctg ggaggccagc
     5761 accgagagga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
     5821 ctgaaggacg gcggccacta cgacgccgag gtgaagacca cctacaaggc caagaagccc
     5881 gtgcagctgc ccggcgccta caacgtggac atcaagctgg acatcctgag ccacaacgag
     5941 gactacacca tcgtggagca gtacgagagg gccgagggca ggcacagcac cggcggcatg
     6001 gacgagctgt acaagtgaac gcgttaagtc gacaatcaac ctctggatta caaaatttgt
     6061 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     6121 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     6181 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     6241 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     6301 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     6361 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     6421 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     6481 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     6541 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     6601 tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag
     6661 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     6721 gacaagatcc tgcaggttaa ttaataaccg ccacgccgca tctgagggcc tatttcccat
     6781 gattccttca tatttgcata tacgatacaa ggctgttaga gagataatta gaattaattt
     6841 gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg
     6901 gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta ccgtaacttg
     6961 aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaaaca ccggagacgg
     7021 ttgtaaatga gcacacaaaa tacacatgct aaaatattat attctatgac ctttataaaa
     7081 tcaaccaaaa tcttcttttt aataacttta gtatcaataa ttagaatttt tatgttcctt
     7141 tttgcaaact tttaataaaa atgagcaaaa taaaaaaacg ctagttttag taactcgcgt
     7201 tgttttcttc acctttaata atagctactc caccacttgt tcctaagcgg tcagctcctg
     7261 cttcaatcat tttttgagca tcttcaaatg ttctaactcc accagctgct ttaactaaag
     7321 cattgtcttt aacaactgac ttcattagtt taacatcttc aaatgttgca cctgattttg
     7381 aaaatcctgt tgatgtttta acaaattcta atccagcttc aacagctatt tcacaagctt
     7441 tcatgatttc ttcttttgtt aataaacaat tttccataat acatttaaca acatgtgatc
     7501 cagctgcttt ttttacagct ttcatgtctt ctaaaactaa ttcataattt ttgtctttta
     7561 atgcaccaat atttaatacc atatcaattt ctgttgcacc atctttaatt gcttcagaaa
     7621 cttcgaatgc ttttgtagct gttgtgcatg cacctagagg aaaacctaca acatttgtta
     7681 ttcctacatt tgtgcctttt aataattctt tacaatagct tgttcaatat gaattaacac
     7741 aaactgttgc aaaatcaaat tcaattgctt catcacataa ttgtttaatt tcagctttcg
     7801 tagcatcttg ttttaataat gtgtgatcta tatatttgtt tagtttcatt ttttctccta
     7861 tatattcatt tttaatttta attctttaat aatttcgtct actttaactt tagcgttttg
     7921 aacagattca ccaacaccta taaaataaat ttttagttta ggttcagttc cacttgggcg
     7981 aacagcaaat catgacttat cttctaaata aaattttagt aagtcttgtc ctggcatatt
     8041 atacattcca tcgatgtagt cttcaacatt aacaacttta agtccagcaa tttgagttaa
     8101 gggtgttgct ctcaatgatt tcattaatgg ttcaattttt aatttctttt cttctggttt
     8161 aaaattcaag tttaaagtga aagtgtaata tgcacccatt tctttaaata aatcttctaa
     8221 atagtctact aatgttttat tttgtttttt ataaaatcaa gcagcctctg ctattaatat
     8281 agaagcttgt attccatctt tatctctagc tgagtcatca attacatatc cataactttc
     8341 ttcataagca aaaacaaaat ttaatccgtt atcttcttct ttagcaattt ctctacccat
     8401 tcatttaaat ccagttaaag tttttacaat attaactcca tatttttcat gagcgattct
     8461 atcacccaaa tcacttgtta caaaacttga atatagagcc ggattttttg gaatgctatt
     8521 taagcgtttt agatttgata attttcaatc aattaaaatt ggtcctgttt gatttccatc
     8581 taatcttaca aaatgaccat catgttttat tgccattcca aatctgtcag catctgggtc
     8641 attcataata ataatatctg catcatgttt aataccatat tcaagcggta tttttcatgc
     8701 aggatcaaat tctggatttg gatttacaac atttttaaat gtttcatctt caaatgcatg
     8761 ctcttcaacc tcaataacgt tatatcctga ttcacgtaat atttttgggg taaatttagt
     8821 tcctgttcca ttaactgcgc taaaaataat ttttaaatct tttttagctt cttgctcttt
     8881 tttgtacgtc tctgtttcag agctaagctg gaaacagcat agcaagttga aataaggcta
     8941 gaccgttaac aacttggccg ctttaaggcc ggtcctagca aggccaagtg gcaccgagtc
     9001 ggtgcttttt ttcgcgggta ttggcctcag cttaattaac ctgcaggtgc tttttgcttg
     9061 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     9121 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     9181 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     9241 tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     9301 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
     9361 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     9421 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
     9481 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     9541 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     9601 ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg
     9661 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     9721 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     9781 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg
     9841 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     9901 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     9961 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
    10021 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
    10081 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
    10141 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
    10201 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
    10261 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat
    10321 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
    10381 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
    10441 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
    10501 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
    10561 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
    10621 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
    10681 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
    10741 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
    10801 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
    10861 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
    10921 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
    10981 actattaact ggcgaactac ttactctagc ttcccgg
//
