LOCUS       sequence-462897-       12268 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12268)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12268)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1286
FEATURES             Location/Qualifiers
     source          1..12268
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    8125..8713
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8178..8198)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8596..8607)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8785..9018
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9096..9217
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9133..9152)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9187..9206
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9257..9392
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9412..9431)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9413..9431)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9441..9458)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9441..9457)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9450..9472)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9599..10054
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9686..9705)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9896..9917
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10080..10184
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10185..10253,10254..11045)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10403..10422)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11216..11804
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11705..11724
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11958..11975
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12092..12113
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12128..12133,12134..12151,12152..12158)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12166..12182
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12171..12193
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12190..12206
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12190..12206
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12225..12245
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12227..12245
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgtct cggcgcaagc aggccaagcc
     4141 ccagcacctc aagtcggacg aggagctgct gccgcctgac ggggctcccg agcacgccgc
     4201 cccgggggaa ggtgcggagg acgcagacag cgggcccgag agccgcagcg ggggcgagga
     4261 gaccagcgtg tgcgagaaat gctgcgccga gttcttcaag tgggcggact tcctggagca
     4321 ccagcggagc tgcaccaagc tcccgcccgt gctgatcgtg cacgaggacg cgcccgcgcc
     4381 gccccccgag gacttccccg agccttcgcc cgccagctcc cccagcgagc gcgccgaaag
     4441 cgaggcggcc gaggaggcgg gtgcggaggg cgcggagggc gaggccaggc cggtggagaa
     4501 ggaggccgag cccatggacg cggaacccgc gggggacacg cgcgcgcccc ggcccccgcc
     4561 tgcggcccct gcacccccaa cgcccgccta cggcgcgccc agcaccaacg tgaccctgga
     4621 ggcgctgctg agcaccaagg tggcggtggc gcagttctcg cagggcgcgc gcgcggcagg
     4681 cggctcggga gcaggtggag gcgtggcagc tgcagccgtg cccctgatcc tggaacagct
     4741 catggccctg cagcagcagc agatccacca gctgcagctc atcgagcaga tccgcagcca
     4801 ggtggccctc atgcagcgcc cgccgccgcg gccctcactc agccccgcgg ccgccccgag
     4861 cgcaccgggc ccggccccca gccagctgcc cgggctggcc gcgctcccgc tgtcggccgg
     4921 ggcccctgcc gccgccatcg cgggctcggg ccccgccgcc ccggccgcct tcgagggcgc
     4981 gcagccgctg tcccggcccg agtctggcgc cagcaccccc ggcggccctg cggagcccag
     5041 cgcgcccgcc gcccccagcg ccgcccctgc ccccgctgcc cccgccccgg cgccagcgcc
     5101 gcagagcgca gcctcgtcgc agccgcagag cgcatccacg ccgcctgccc tggccccggg
     5161 gtccctgctg ggtgcggcgc ccggcctgcc aagtccgctt ctacctcaga cttccgccag
     5221 cggcgtcatc ttccccaacc cgctggtcag catcgcggcc acggccaacg ctctggaccc
     5281 gctgtccgcg ctcatgaagc accgcaaggg caagccgccc aatgtgtcgg tgttcgagcc
     5341 caaagccagc gccgaggacc cgttcttcaa gcacaaatgc cgcttctgcg ccaaggtctt
     5401 cggcagcgac agcgcgctcc agatccacct gcgctcgcac acaggcgagc ggcccttcaa
     5461 gtgcaacatc tgcgggaacc gcttctccac caaaggcaac ctgaaggtgc acttccagag
     5521 gcacaaggag aagtaccccc acatccagat gaacccttac ccggtccccg agtacctgga
     5581 caacgtgccc acctgctcgg gcatccccta cggcatgtcg ctgccccccg agaagcccgt
     5641 gaccacctgg ctggacagca agcccgtgct gcccaccgtg cccacgtccg tggggctgca
     5701 actgccgccc actgtccctg gcgcgcacgg ctacgccgac tctcccagcg ccaccccagc
     5761 cagccgctcc ccgcagaggc cctcgcccgc ctccagcgag tgcgcctcct tgtccccagg
     5821 cctcaaccac gtggagtccg gcgtgtcggc caccgccgag tccccacagt cgctcctcgg
     5881 cgggccgccc ctcactaaag ccgagcccgt cagcctgccc tgcaccaacg ccagggccgg
     5941 ggacgctccc gtgggcgcgc aggctagcgc tgcacccaca tcggtggacg gcgcacccac
     6001 gagcctcggc agccccgggc tgcccgccgt ctccgagcag ttcaaggccc agtttccgtt
     6061 cggggggctg ctagactcga tgcaaacgtc ggaaacctcg aagctgcagc agctggtgga
     6121 gaacatcgac aagaagatga cggacccgaa ccagtgcgtc atctgccacc gggtgctgag
     6181 ctgccagagc gcgctgaaga tgcactaccg gacgcacacg ggggagcggc cgttcaagtg
     6241 caagatctgc ggccgcgcct tcaccaccaa gggcaacctc aagacgcact tcggcgtgca
     6301 ccgtgcaaag ccgcccctgc gcgtgcagca ctcctgcccc atctgccaga agaagttcac
     6361 caacgccgtg gtcctgcagc agcacatccg catgcacatg ggcggccaga tccccaacac
     6421 gccgctgccg gagggcttcc aggatgccat ggactccgag ctggcctacg acgacaagaa
     6481 cgcggagacc ctgagcagct acgatgacga catggacgag aactccatgg aggacgacgc
     6541 tgagctgaag gacgcggcca ccgacccggc caagccactc ctgtcctacg cggggtcctg
     6601 cccgccctcc ccgccctcgg tcatctccag cattgccgcc ctggagaacc agatgaagat
     6661 gatcgactcg gtcatgagct gccagcagct gaccggcctc aagtccgtgg agaacgggtc
     6721 cggggagagt gaccgcctga gcaacgactc ctcgtcggcc gtgggcgacc tggagagccg
     6781 cagcgcgggc agccccgccc tgtccgagtc ctcgtcctcg caggccctgt cgccggcccc
     6841 cagcaatggt gagagcttcc gctccaagtc cccgggcctg ggcgccccgg aggagcccca
     6901 ggaaatcccg ctcaagaccg agaggccgga cagcccagcc gccgccccgg gcagcggagg
     6961 cgcccctggc cgcgcgggca tcaaggagga ggcgcccttc agcctgctgt tcctgagcag
     7021 ggagcggggt aagtgtccca gcactgtgtg tggtgtctgt ggcaagcctt ttgcttgcaa
     7081 gagcgcgttg gaaatccact accgcagcca tactaaggag cggccattcg tctgcgcgct
     7141 ctgcaggcga gggtgctcca ctatgggtaa tttaaaacag cacttactga cacacagatt
     7201 gaaagagctg ccttctcagt tatttgaccc caactttgct ctaggtccca gccaaagcac
     7261 tcctagcctg atctccagcg ccgcacccac catgatcaaa atggaagtga acggtcacgg
     7321 caaggccatg gcgctgggcg agggtccccc gctgcccgcg ggcgtccagg tccccgccgg
     7381 gcctcagaca gtgatgggcc cgggcctggc gcccatgctg gcccccccac cgcgccggac
     7441 gcccaagcag cacaactgcc agtcgtgcgg gaagaccttc tcctcggcca gcgccctgca
     7501 gatccatgag cgcacgcaca ccggcgagaa gccgttcggc tgcaccatct gcggccgggc
     7561 cttcaccact aagggcaacc tcaaggtgca catggggaca cacatgtgga ataacgcccc
     7621 cgcgagacgc ggccgccgcc tgtctgtgga gaaccccatg gctctcctag ggggtgatgc
     7681 cctgaagttc tctgaaatgt tccagaagga cctggcagct cgggcaatga acgtcgaccc
     7741 cagtttttgg aaccagtatg ctgcagccat cactaacggg ctcgccatga agaacaacga
     7801 gatctccgtc atccagaacg gcggcatccc ccagctcccc gtgagtcttg ggggcagcgc
     7861 cctcccccct ctgggcagca tggccagtgg gatggacaaa gcacgcactg gcagtagccc
     7921 acccatcgtc agcttggaca aagcgagctc agaaacagca gccagccgcc cattcacgcg
     7981 gtttatcgag gataacaagg agattggtat caactagtaa tgaactagtc cgtaacttga
     8041 aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgagttct taaacgccat
     8101 ctggttctaa cgcgttaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg
     8161 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     8221 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     8281 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     8341 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     8401 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     8461 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     8521 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     8581 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     8641 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     8701 gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     8761 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     8821 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     8881 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     8941 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     9001 tgtggaaaat ctctagcagt acgtatagta gttcatgtca tcttattatt cagtatttat
     9061 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
     9121 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     9181 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
     9241 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     9301 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     9361 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
     9421 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     9481 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     9541 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     9601 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     9661 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     9721 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     9781 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     9841 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     9901 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     9961 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
    10021 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
    10081 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
    10141 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
    10201 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
    10261 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
    10321 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
    10381 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
    10441 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
    10501 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
    10561 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
    10621 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
    10681 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
    10741 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
    10801 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
    10861 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
    10921 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
    10981 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
    11041 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
    11101 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
    11161 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
    11221 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
    11281 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
    11341 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
    11401 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
    11461 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
    11521 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
    11581 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
    11641 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
    11701 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
    11761 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
    11821 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
    11881 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
    11941 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
    12001 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
    12061 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
    12121 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
    12181 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
    12241 aaagggaaca aaagctggag ctgcaagc
//
