LOCUS       sequence-46291-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Sinha H, Nicholson BP, Steinmetz LM, McCusker JH
  TITLE     Complex genetic interactions in a quantitative trait locus.
  JOURNAL   PLoS Genet. 2006 Feb;2(2):e13. Epub 2006 Feb 3.
  PUBMED    16462944
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2006-02"; volume: "2(2)"; pages: "e13. Epub 2006 Feb
            3"
COMMENT     Sequence Label: pSA41
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(72..269)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(275..847)
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
ORIGIN
        1 ggatctgata tcatcgatga attcgagctc gttttcgaca ctggatggcg gcgttagtat
       61 cgaatcgaca gcagtatagc gaccagcatt cacatacgat tgacgcatga tattactttc
      121 tgcgcactta acttcgcatc tgggcagatg atgtcgaggc gaaaaaaaat ataaatcacg
      181 ctaacatttg attaaaatag aacaactaca atataaaaaa actatacaaa tgacaagttc
      241 ttgaaaacaa gaatcttttt attgtcagta ctgattaggg gcagggcatg ctcatgtaga
      301 gcgcctgctc gccgtccgag gcggtgccgt cgtacagggc ggtgtccagg ccgcagaggg
      361 tgaaccccat ccgccggtac gcgtggatcg ccggtgcgtt gacgttggtg acctccagcc
      421 agaggtgccc ggcgccccgc tcgcgggcga actccgtcgc gagccccatc aacgcgcgcc
      481 cgaccccgtg cccccggtgc tccggggcga cctcgatgtc ctcgacggtc agccggcggt
      541 tccagccgga gtacgagacg accacgaagc ccgccaggtc gccgtcgtcc ccgtacgcga
      601 cgaacgtccg ggagtccggg tcgccgtcct ccccgtcgtc cgattcgtcg tccgattcgt
      661 cgtcggggaa caccttggtc aggggcgggt ccaccggcac ctcccgcagg gtgaagccgt
      721 ccccggtggc ggtgacgcgg aagacggtgt cggtggtgaa ggacccatcc agtgcctcga
      781 tggcctcggc gtcccccggg acactggtgc ggtaccggta agccgtgtcg tcaagagtgg
      841 tacccatggt tgtttatgtt cggatgtgat gtga
//
