LOCUS       sequence-46293-e       10074 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10074)
  AUTHORS   Lee DY, Sugden B
  TITLE     The LMP1 oncogene of EBV activates PERK and the unfolded protein 
            response to drive its own synthesis.
  JOURNAL   Blood. 2008 Feb 15;111(4):2280-9. Epub 2007 Nov 27.
  PUBMED    18042799
REFERENCE   2  (bases 1 to 10074)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood."; 
            date: "2008-02-15"; volume: "111(4)"; pages: "2280-9. Epub 2007 Nov 
            27"
COMMENT     Sequence Label: pCMMP-dnPERK-IRES-eGFP
FEATURES             Location/Qualifiers
     source          1..10074
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        84..387
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        388..591
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     542..562
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    813..1170
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1235..1651
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1581..1603
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1619..1635
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     RBS             4137..4145
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     misc_feature    4890..5455
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4906..5457
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5050..5067)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5277..5296
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5469..5471,5472..5474,5475..6188)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5514..5535)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5775..5794)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6122..6143
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(7455..7471)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7455..7471)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7468..7490)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7479..7495
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7503..7509,7510..7527,7528..7533))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7548..7569
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7686..7703)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7857..8445)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7937..7956)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8616..9407,9408..9476))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9239..9258
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9477..9581)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9649..9667
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9705..9727)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9827..9846
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10040..10062
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10054..10071
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10055..10071
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactagtta ttaatagtaa tcaattacgg ggtcattagt
       61 tcatagccca tattggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      121 ccgcccaacg acccccgccc cttgacgtca ataatgacgt atgttcccat agtaacgcca
      181 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      241 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      301 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      361 tacgtattag tcatcgctat taccatggtg atgcggttgt ggcagtacat caatgggcgt
      421 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
      481 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
      541 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg
      601 aaccggcgcc agtcctccga tagactgcgt cgcccgggta cccgtgttct caataaaccc
      661 tcttgcagtt gcatccgact cgtggtctcg ctgttccttg ggagggtctc ctctgagtga
      721 ttgactaccc gtcagcgggg tctttcattt ggaggttcca ccgagatttg gagacccctg
      781 cccagggacc accgaccccc ccgccgggag gtaagctggc cagcaactta tctgtgtctg
      841 tccgattgtc tagtgtctat gactgatttt atgcgcctgc gtcggtacta gttagctaac
      901 tagctctgta tctggcggac ccgtggtgga actgacgagt tcggaacacc cggccgcaac
      961 cctgggagac gtcccaggga cttcgggggc cgtttttgtg gcccgacctg agtcctaaaa
     1021 tcccgatcgt ttaggactct ttggtgcacc ccccttagag gagggatatg tggttctggt
     1081 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggga
     1141 ccgaagccgc gccgcgcgtc ttgtctgctg cagcatcgtt ctgtgttgtc tctgtctgac
     1201 tgtgtttctg tatttgtctg aaaatatggg cccgggctag actgttacca ctcccttaag
     1261 tttgacctta ggtcactgga aagatgtcga gcggatcgct cacaaccagt cggtagatgt
     1321 caagaagaga cgttgggtta ccttctgctc tgcagaatgg ccaaccttta acgtcggatg
     1381 gccgcgagac ggcaccttta accgagacct catcacccag gttaagatca aggtcttttc
     1441 acctggcccg catggacacc cagaccaggt cccctacatc gtgacctggg aagccttggc
     1501 ttttgacccc cctccctggg tcaagccctt tgtacaccct aagcctccgc ctcctcttcc
     1561 tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg accccgcctc gatcctccct
     1621 ttatccagcc ctcactcctt ctctaggcgc ccccatatgg ccatatgaga tcttatatgg
     1681 ggcacccccg ccccttgtaa acttccctga ccctgacatg acaagagtta ctaacagccc
     1741 ctctctccaa gctcacttac aggctctcta cttagtccag cacgaagtct ggagacctct
     1801 ggcggcagcc taccaagaac aactggaccg accggtcaat tggttcgagc tcggatccac
     1861 tagtaacggc cgccagtgtg ctggaattct gcagatatcc atcacactgg cggccgctcg
     1921 acgatggagc gcgccatcag cccggggctg ctggtacggg cgctgctgct gctgctgctg
     1981 ctggggctcg cggcaaggac ggtggccgcg gggcgcgccc gtggcctccc agcgccgacg
     2041 gcggaggcgg cgttcggcct cggggcggcc gctgctccca cctcagcgac gcgagtaccg
     2101 gcggcgggcg ccgtggctgc ggccgaggtg actgtggagg acgctgaggc gctgccggca
     2161 gccgcgggag agcaggagcc tcggggtccg gaaccagacg atgagacaga gttgcgaccg
     2221 cgcggcaggt cattagtaat tatcagcact ttagatggga gaattgctgc cttggatcct
     2281 gaaaatcatg gtaaaaagca gtgggatttg gatgtgggat ccggttcctt ggtgtcatcc
     2341 agccttagca aaccagaggt atttgggaat aagatgatca ttccttccct ggatggagcc
     2401 ctcttccagt gggaccgaga ccgtgaaagc atggaaacag ttcctttcac agttgaatca
     2461 cttcttgaat cttcttataa atttggagat gatgttgttt tggttggagg aaaatctctg
     2521 actacatatg gactcagtgc atatagtgga aaggtgaggt atatctgttc agctctgggt
     2581 tgtcgccaat gggatagtga cgaaatggaa caagaggaag acatcctgct tctacagcgt
     2641 acccaaaaaa ctgttagagc tgtcggacct cgcagtggca atgagaagtg gaatttcagt
     2701 gttggccact ttgaacttcg gtatattcca gacatggaaa cgagagccgg atttattgaa
     2761 agcaccttta agcccaatga gaacacagaa gagtctaaaa ttatttcaga tgtggaagaa
     2821 caggaagctg ccataatgga catagtgata aaggtttcgg ttgctgactg gaaagttatg
     2881 gcattcagta agaagggagg acatctggaa tgggagtacc agttttgtac tccaattgca
     2941 tctgcctggt tacttaagga tgggaaagtc attcccatca gtctttttga tgatacaagt
     3001 tatacatcta atgatgatgt tttagaagat gaagaagaca ttgtagaagc tgccagagga
     3061 gccacagaaa acagtgttta cttgggaatg tatagaggcc agctgtatct gcagtcatca
     3121 gtcagaattt cagaaaagtt tccttcaagt cccaaggctt tggaatctgt cactaatgaa
     3181 aacgcaatta ttcctttacc aacaatcaaa tggaaaccct taattcattc tccttccaga
     3241 actcctgtct tggtaggatc tgatgaattt gacaaatgtc tcagtaatga taagttttct
     3301 catgaagaat atagtaatgg tgcactttca atcttgcagt atccatatga taatggttat
     3361 tatctaccat actacaagag ggagaggcac aaacgaagca cacagattac agtcagattc
     3421 ctcgacaacc cacattacaa caagaatatc cgcaaaaagg atcctgttct tcttttacac
     3481 tggtggaaag aaatagttgc aacgattttg ttttgtatca tagcaacaac gtttattgtg
     3541 cgcaggcttt tccatcctca tcctcacagg caaaggaagg agtctgaaac tcagtgtcaa
     3601 actgaaaata aatatgattc tgtaagtggt gaagccaatg acagtagctg gaatgacata
     3661 aaaaactctg gatatatatc acgatatcta actcccatgg aacgacctga agctataaac
     3721 atcattgaaa atgctgtatt tgaggacttg gactttccag gaaaaacagt gctcagacag
     3781 aggtctcgct ccttgagttc atcgggaaca aaacattcaa gacagtccaa caactcccat
     3841 agccctttgc caagcaatta gccttaagtt gtgctagcaa ccctaatagg tgatgcagat
     3901 aatagcctac ttcttagaat atgcctgtcc aaaattgcag acttgaaaag tttgttcttc
     3961 gctcaatttt tttgtggact acttttttta tatcaaattt aagctggatt tgggggcata
     4021 acctaatttg agccaactcc tgagttttgc tatacttaag gaaagggcta tctttgttct
     4081 ttgttagtct cttgaaactg gctgctggcc aagctttata gccctcacca tttgcctaag
     4141 gaggtagcag caatccctaa tatatatata tagtgagaac taaaatggat atatttttat
     4201 aatgcagaag aaggaaagtc cccctgtgtg gtaactgtat tgttctagaa atatgctttc
     4261 tagagatatg atgattttga aactgatttc tagaaaaagc tgactccatt tttgtccctg
     4321 gcgggtaaat taggaatctg cactattttg gaggacaagt agcacaaact gtataacggt
     4381 ttatgtccgt agttttatag tcctatttgt agcattcaat agctttattc cttagatggt
     4441 tctagggtgg gtttacagct ttttgtactt ttacctccaa taaagggaaa atgaagcttt
     4501 ttatgtaaat tggttgaaag gtctagtttt gggaggaaaa aagccgtagt aagaaatgga
     4561 tcatatatat tacaactaac ttcttcaact atggactttt taagcctaat gaaatcttaa
     4621 gtgtcttata tgtaatcctg taggttggta cttcccccaa actgattata ggtaacagtt
     4681 taatcatctc acttgctaac atgtttttat ttttcactgt aaatatgttt atgttttatt
     4741 tataaaaatt ctgaaatcaa tccatttggg ttggtggtgt acagaacaca cttaagtgtg
     4801 ttaacttgtg acttctttca agtctaaatc atttaataaa acttttttta aattaaaaaa
     4861 aaaaaaaaaa aaactcgagg atccggaatt ccgccccccc ccctaacgtt actggccgaa
     4921 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt
     4981 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg
     5041 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc
     5101 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc
     5161 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa
     5221 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc
     5281 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg
     5341 gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaacg
     5401 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg
     5461 ccacaaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     5521 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     5581 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     5641 ggcccaccct cgtgaccacc ttcacctacg gcgtgcagtg cttcagccgc taccccgacc
     5701 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     5761 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     5821 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     5881 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     5941 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     6001 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     6061 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     6121 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     6181 acaagtaatg aattaattaa gaattccgga ttagtccaat ttgttaaaga caggatatca
     6241 gtggtccagg ctctagtttt gactcaacaa tatcaccagc tgaagcctat agagtacgag
     6301 ccatagataa aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc
     6361 acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaatacat
     6421 aactgagaat agagaagttc agatcaaggt taggaacaga gagacagcag aatatgggcc
     6481 aaacaggata tctgtggtaa gcagttcctg ccccgctcag ggccaagaac agttggaaca
     6541 ggagaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca
     6601 agaacagatg gtccccagat gcggtcccgc cctcagcagt ttctagagaa ccatcagatg
     6661 tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag
     6721 ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa
     6781 cccctcactc ggcgcgccag tcctccgata gactgcgtcg cccgggtacc cgtgttctca
     6841 ataaaccctc ttgcagttgc atccgactcg tggtctcgct gttccttggg agggtctcct
     6901 ctgagtgatt gactacccgt cagcggggtc tttcagtttc tcccacctac acaggtctca
     6961 ctaacattcc tgatgtgccg cagggactcc gtcagcccgg tttttgttta taataaaatg
     7021 caagaacagt gttcccttca agccagacta catcctgact ctcggcttta taaaagaatg
     7081 ttgaagggct ctgtggacta tctgccacac gactttttaa gatttttatg cctcctggat
     7141 gagggattta gtcaatctat cctcgtctat tttgctggct tctccgtatt ttaaatttct
     7201 agtttgcact cccttcctga gagcacggcg attgcagagt agttaatact ctgagggcag
     7261 gcttctgtga aaaggttgcc tgggctcagt gtgagatttt gccataaaaa ggggtcctgc
     7321 ccctgtgtac agacagatcg gaatctagag tgcatactca gagtccccgc ggttccgggg
     7381 ctctgatctc agggcatctt tgcctagaga tcctctacgc cggacgcatc gtggccctcg
     7441 aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     7501 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     7561 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     7621 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     7681 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     7741 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     7801 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     7861 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     7921 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     7981 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     8041 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     8101 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     8161 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     8221 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     8281 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     8341 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     8401 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     8461 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     8521 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     8581 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     8641 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     8701 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     8761 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     8821 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     8881 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     8941 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     9001 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     9061 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     9121 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     9181 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     9241 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     9301 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     9361 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     9421 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     9481 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     9541 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     9601 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     9661 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     9721 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     9781 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     9841 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     9901 taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     9961 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
    10021 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgcc
//
