LOCUS       sequence-462939-       11599 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11599)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11599)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0512
FEATURES             Location/Qualifiers
     source          1..11599
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    7456..8044
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7509..7529)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7927..7938)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8116..8349
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8427..8548
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8464..8483)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8518..8537
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8588..8723
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8743..8762)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8744..8762)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8772..8789)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8772..8788)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8781..8803)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8930..9385
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9017..9036)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9227..9248
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9411..9515
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9516..9584,9585..10376)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9734..9753)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10547..11135
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11036..11055
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11289..11306
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11423..11444
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11459..11464,11465..11482,11483..11489)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11497..11513
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11502..11524
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11521..11537
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11521..11537
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11556..11576
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11558..11576
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgccg aggaggaagc agcaggcccc
     4141 ccggcgctcg gcagcttatg ttcctgagga agaattgaag gcagcagaaa tagatgaaga
     4201 gcacgtggag gatgacgggc tgtctttgga cattcaggaa agtgagtaca tgtgcaatga
     4261 agagacggag atcaaagagg cgcagagcta ccagaactcc ccagtcagct ctgcgactaa
     4321 ccaggacgcc ggctacgggt cgcccttcag tgagagcagc gaccagctag cccatttcaa
     4381 aggctcttcc tctcgagaag agaaggagga tccgcagtgt cccgacagcg tctcgtaccc
     4441 ccaggacagc ctggcacaga tcaaagctgt gtatgcaaac ttgttctccg agtcctgctg
     4501 gtccagctta gctctggatt taaagaagtc gggttccacc accagcacca acgatgccag
     4561 ccagaaggag agctccgccc ccacccccac accccccacc tgccccgtca gcaccactgg
     4621 ccccaccacg agcacgccca gcaccagctg cagctccagc accagccaca gcagtaccac
     4681 cagtaccagc agcagctccg ggtacgactg gcaccaggct gcactggcca agacgctgca
     4741 gcagacgtcc tcgtatgggc tgcttcctga gcccagcctg ttcagcaccg tgcagctcta
     4801 ccgccagaac aacaagctct acggctccgt cttcacgggc gccagcaagt tccggtgcaa
     4861 agactgcagt gccgcgtacg acacgctggt ggaactgacg gtgcacatga acgagacagg
     4921 ccactaccgt gacgacaaca gggacaagga ctccgagaag accaagaggt ggtccaagcc
     4981 caggaagcgc tccctgatgg agatggaggg gaaggaggat gcccagaagg tgctgaagtg
     5041 catgtactgt ggacactcct ttgagtcctt gcaggacctc agcgtccaca tgatcaaaac
     5101 caagcattac cagaaagtgc ctctgaagga gccagtgcca gccatcacca aactggtccc
     5161 ctccaccaaa aagcgggcgc ttcaggacct ggcgcccccc tgctcccctg agccagcagg
     5221 aatggccgca gaggtggccc tgagtgagtc agccaaggat cagaaagcag cgaacccgta
     5281 cgtcacgccc aataaccgct atggctacca gaatggcgcc agctacacct ggcagtttga
     5341 ggcccgcaag gcgcagatcc tcaagtgcat ggagtgtggc agctcccacg acacgctgca
     5401 gcagctcacc gcccacatga tggtcaccgg gcacttcctg aaagtgacca cctcggcttc
     5461 taagaagggc aagcagttgg tgctggaccc tgtggtggaa gagaagatcc agtccatccc
     5521 actaccgccc accacccaca cgcggctgcc ggcctccagc atcaaaaagc agcccgactc
     5581 tcccgcgggg tccacgactt ctgaagaaaa gaaagagcca gagaaggaga agccgcctgt
     5641 ggctggcgac gcggagaaga tcaaggagga gagtgaggac agcttggaga aatttgagcc
     5701 cagcaccctg tacccgtacc tgcgtgagga ggacctggac gacagcccca agggagggct
     5761 ggacattctc aagtccctgg agaataccgt ctccacggcc attagcaaag ctcagaatgg
     5821 tgcgccctca tggggtggct accccagcat ccatgcagcc taccagctcc cgggcaccgt
     5881 gaagccactg ccggcggccg tgcagagcgt gcaggtgcag ccgtcctatg ctggcggcgt
     5941 gaagtcgctg tcttccgccg agcacaacgc cctcctgcac tccccaggga gcctcacgcc
     6001 cccaccgcac aagagcaacg tgtctgccat ggaggagctg gtggagaagg tcacgggcaa
     6061 ggtcaacatc aagaaggagg agagaccccc tgagaaggag aagagctccc tggccaaggc
     6121 tgcgtccccc atagcaaaag agaataaaga tttcccgaaa acggaggaag tcagcggcaa
     6181 accacagaag aagggccctg aggccgagac tgggaaggcc aaaaaggagg gaccgctgga
     6241 cgttcacacc ccaaatggca cagagcctct caaagcaaag gtcaccaacg gctgtaacaa
     6301 cctggggatc atcatggacc actcaccgga gccttccttc atcaacccgc tgagcgcttt
     6361 gcagtccatc atgaacaccc acctgggcaa ggtgtccaag cccgtgagtc cctcgctgga
     6421 cccgctggcg atgctgtaca agatcagcaa cagcatgctg gacaagccgg tgtaccccgc
     6481 cacccctgtg aagcaggccg atgccatcga ccgctactat tatgaaaaca gcgaccagcc
     6541 cattgactta accaagtcca agaacaagcc gctggtgtcc agcgtggctg attcggtggc
     6601 atcacctctg cgggagagcg cactcatgga catctccgac atggtgaaaa acctcacagg
     6661 ccgcctgacg cccaagtcct ccacgccctc cacagtttca gagaagtccg atgctgatgg
     6721 cagcagcttt gaggaggcgt tggacgagct gtcaccggtc cacaagagga agggccggca
     6781 gtccaactgg aacccgcagc accttctcat cctgcaggcc cagttcgcct cgagcttgcg
     6841 ggagaccaca gagggcaagt acatcatgtc ggacttgggc ccgcaggaga gggtgcacat
     6901 ctcgaagttt actgggctct ccatgaccac catcagccac tggctggcca atgtgaagta
     6961 ccagttgagg aggacagggg gaacgaaatt cctaaagaac ctggacacag ggcatcctgt
     7021 tttcttttgc aacgattgtg cctctcagtt cagaactgct tctacataca taagtcattt
     7081 ggagacacac ttgggcttca gcctgaagga tctctccaag ctgccactca atcagattca
     7141 agaacagcag aatgtttcga aagtcctcac caacaaaact ctgggcccac tgggggccac
     7201 cgaggaagac ttgggctcca cattccaatg taagctctgc aaccggactt ttgcgagcaa
     7261 gcacgcagtc aaactgcacc ttagtaagac ccacggcaag tctcccgagg accacctgat
     7321 ctatgtgact gagttggaga aacagtagta atgaactagt ccgtaacttg aaagtatttc
     7381 gatttcttgg ctttatatat cttgtggaaa ggacgaggga ggtgttacca tgtatcttgc
     7441 acgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     7501 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     7561 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     7621 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     7681 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     7741 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     7801 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     7861 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     7921 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     7981 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     8041 ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     8101 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     8161 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     8221 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     8281 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     8341 tctctagcag tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa
     8401 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     8461 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     8521 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     8581 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     8641 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     8701 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     8761 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     8821 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     8881 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     8941 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     9001 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     9061 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     9121 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     9181 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     9241 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     9301 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     9361 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     9421 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     9481 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     9541 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     9601 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     9661 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     9721 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     9781 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     9841 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     9901 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     9961 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
    10021 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
    10081 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
    10141 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
    10201 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
    10261 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
    10321 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
    10381 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
    10441 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
    10501 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
    10561 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
    10621 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
    10681 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
    10741 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
    10801 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
    10861 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
    10921 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
    10981 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
    11041 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
    11101 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
    11161 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
    11221 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
    11281 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
    11341 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
    11401 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
    11461 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
    11521 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
    11581 aaaagctgga gctgcaagc
//
