LOCUS       sequence-462947-       10867 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10867)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2197
FEATURES             Location/Qualifiers
     source          1..10867
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6724..7312
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6777..6797)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7195..7206)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7384..7617
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7695..7816
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7732..7751)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7786..7805
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7856..7991
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8011..8030)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8012..8030)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8040..8057)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8040..8056)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8049..8071)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8198..8653
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8285..8304)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8495..8516
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8679..8783
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8784..8852,8853..9644)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9002..9021)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9815..10403
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10304..10323
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10557..10574
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10691..10712
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10727..10732,10733..10750,10751..10757)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10765..10781
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10770..10792
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10789..10805
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10789..10805
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10824..10844
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10826..10844
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggcc ggcgcgctgg caggtctggc
     4141 cgcgggcttg caggtcccgc gggtcgcgcc cagcccagac tcggactcgg acacagactc
     4201 ggaggacccg agtctccggc gcagcgcggg cggcttgctc cgctcgcagg tcatccacag
     4261 cggtcacttc atggtgtcgt cgccgcacag cgactcgctg ccccggcggc gcgaccagga
     4321 ggggtccgtg gggccctccg acttcgggcc gcgcagtatc gaccccacac tcacacgcct
     4381 cttcgagtgc ttgagcctgg cctacagtgg caagctggtg tctcccaagt ggaagaattt
     4441 caaaggcctc aagctgctct gcagagacaa gatccgcctg aacaacgcca tctggagggc
     4501 ctggtatatc cagtatgtga agcggaggaa gagccccgtg tgtggcttcg tgacccccct
     4561 gcaggggcct gaggctgatg cgcaccggaa gccggaggcc gtggtcctgg aggggaacta
     4621 ctggaagcgg cgcatcgagg tggtgatgcg ggaataccac aagtggcgca tctactacaa
     4681 gaagcggctc cgtaagccca gcagggaaga tgacctcctg gcccctaagc aggcggaagg
     4741 caggtggccg ccgccggagc aatggtgcaa acagctcttc tccagtgtgg tccccgtgct
     4801 gctgggggac ccagaggagg agccgggtgg gcggcagctc ctggacctca attgcttttt
     4861 gtccgacatc tcagacactc tcttcaccat gactcagtcc ggcccttcgc ccctgcagct
     4921 gccgcctgag gatgcctacg tcggcaatgc tgacatgatc cagccggacc tgacgccact
     4981 gcagccaagc ctggatgact tcatggacat ctcagatttc tttaccaact cccgcctccc
     5041 acagccgccc atgccttcaa acttcccaga gccccccagc ttcagccccg tggttgactc
     5101 cctcttcagc agtgggaccc tgggcccaga ggtgcccccg gcttcctcgg ccatgaccca
     5161 cctctctgga cacagccgtc tgcaggctcg gaacagctgc cctggcccct tggactccag
     5221 cgccttcctg agttctgatt tcctccttcc tgaagacccc aagccccggc tcccaccccc
     5281 tcctgtaccc ccacctctgc tgcattaccc tccccctgcc aaggtgccag gcctggagcc
     5341 ctgcccccca cctcccttcc ctcccatggc accacccact gctttgctgc aggaagagcc
     5401 tctcttctct cccaggtttc ccttccccac cgtccctcct gccccaggag tgtctccgct
     5461 gcctgctcct gcagccttcc cacccacccc acagtctgtc cccagcccag cccccacccc
     5521 cttccccata gagcttctac ccttggggta ttcggagcct gcctttgggc cttgcttctc
     5581 catgcccaga ggcaagcccc ccgccccatc ccctagggga cagaaagcca gcccccctac
     5641 cttagcccct gccactgcca gtccccccac cactgcgggg agcaacaacc cctgcctcac
     5701 acagctgctc acagcagcta agccggagca agccctggag ccaccacttg tatccagcac
     5761 cctcctccgg tccccagggt ccccgcagga gacagtccct gaattcccct gcacattcct
     5821 tcccccgacc ccggccccta caccgccccg gccacctcca ggcccggcca cattggcccc
     5881 ttccaggccc ctgcttgtcc ccaaagcgga gcggctctca cccccagcgc ccagcggcag
     5941 tgaacggcgg ctgtcagggg acctcagctc catgccaggc cctgggactc tgagcgtccg
     6001 tgtctctccc ccgcaaccca tcctcagccg gggccgtcca gacagcaaca agaccgagaa
     6061 ccggcgtatc acacacatct ccgcggagca gaagcggcgc ttcaacatca agctggggtt
     6121 tgacaccctt catgggctcg tgagcacact cagtgcccag cccagcctca aggagcgtgc
     6181 gggcttgcag gaggaggccc agcagctgcg ggatgagatt gaggagctca atgccgccat
     6241 taacctgtgc cagcagcagc tgcccgccac aggggtaccc atcacacacc agcgttttga
     6301 ccagatgcga gacatgtttg atgactacgt ccgaacccgt acgctgcaca actggaagtt
     6361 ctgggtgttc agcatcctca tccggcctct gtttgagtcc ttcaacggga tggtgtccac
     6421 ggcaagtgtg cacaccctcc gccagacctc actggcctgg ctggaccagt actgctctct
     6481 gcccgctctc cggccaactg tcctgaactc cctacgccag ctgggcacat ctaccagtat
     6541 cctgaccgac ccgggccgca tccctgagca agccacacgg gcagtcacag agggcaccct
     6601 tggcaaacct ttatagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct
     6661 ttatatatct tgtggaaagg acgaaaacta atatcggcac gctctggtac gcgttaagtc
     6721 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     6781 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     6841 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     6901 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     6961 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     7021 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     7081 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     7141 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     7201 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     7261 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcgtcgactt
     7321 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     7381 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     7441 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     7501 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     7561 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     7621 cgtatagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     7681 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     7741 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     7801 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     7861 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     7921 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     7981 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     8041 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     8101 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     8161 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     8221 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     8281 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     8341 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     8401 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     8461 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     8521 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     8581 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     8641 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     8701 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     8761 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     8821 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     8881 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     8941 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     9001 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     9061 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     9121 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     9181 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     9241 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     9301 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     9361 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     9421 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     9481 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     9541 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     9601 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     9661 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     9721 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     9781 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     9841 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     9901 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     9961 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
    10021 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
    10081 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
    10141 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
    10201 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
    10261 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
    10321 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
    10381 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
    10441 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
    10501 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
    10561 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
    10621 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
    10681 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
    10741 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
    10801 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
    10861 tgcaagc
//
