LOCUS       sequence-462956-        4061 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Random transposon mutagenesis vector for Bacteroidales species - 
            chloramphenicol.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4061)
  TITLE     A toolkit for transposon libraries and functional genomics in 
            intestinal Bacteroidales species
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCV019-catP
FEATURES             Location/Qualifiers
     source          1..4061
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(936..978)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      2211..2599
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            2714..2823
                     /note="incP origin of transfer"
     CDS             complement(join(2987..3778,3779..3847))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3610..3629
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3848..3952)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4020..4038
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cctaacaggt tggatgataa gtccccggtc ttcgtagata aaacgaaagg ctcagtaaag
       61 agttaaggaa agtgaagcat cttcgatgct ggagtgggat atactaaatt acctaaaaaa
      121 gtggcgcaca aatttgcgcg ccacaattat tattcatacc tttgtggacc gtattacaaa
      181 gaacccaatc atatggtatt tgaaaaaatt gataaaaata gttggaacag aaaagagtat
      241 tttgaccact actttgcaag tgtaccttgt acatacagca tgaccgttaa agtggatatc
      301 acacaaataa aggaaaaggg aatgaaacta tatcctgcaa tgctttatta tattgcaatg
      361 attgtaaacc gccattcaga gtttaggacg gcaatcaatc aagatggtga attggggata
      421 tatgatgaga tgataccaag ctatacaata tttcacaatg atactgaaac attttccagc
      481 ctttggactg agtgtaagtc tgactttaaa tcatttttag cagattatga aagtgatacg
      541 caacggtatg gaaacaatca tagaatggaa ggaaagccaa atgctccgga aaacattttt
      601 aatgtatcta tgataccgtg gtcaaccttc gatggcttta atctgaattt gcagaaagga
      661 tatgattatt tgattcctat ttttactatg gggaaatatt ataaagaaga taacaaaatt
      721 atacttcctt tggcaattca agttcatcac gcagtatgtg acggatttca catttgccgt
      781 tttgtaaacg aattgcagga attgataaat agttaactgc agggatgtcc acgaggtgta
      841 tgagacgtca gaattggtta attcgtctct cagaattggg agtctacgaa gaccggggac
      901 ttatcatcca acctgttaga aaggcgatga aagagataaa acgaaaggct cagtcgaaag
      961 actgggcctt tcgttttaca attgggctac cttttttttg ttttgtttgc aatggttaat
     1021 ctattgttaa aatttaaagt ttcacttgaa ctttcaaata atgttcttat atttgcagtg
     1081 tcgaaagaaa caaagtagga ctgatcgtcc atcaatttaa aatttaaaat aatggaaaaa
     1141 aaggaatttc gtgttttgat aaaatactgt tttctgaagg gaaaaaatac agtggaagca
     1201 aaaacttggc ttgataatga gtttccggac tctgccccag ggaaatcaac aataattgat
     1261 tggtatgcaa aattcaagcg tggtgaaatg agcacggagg acggtgaacg cagtggacgc
     1321 ccgaaagagg tggttaccga cgaaaacatc aaaaaaatcc acaaaatgat tttgaatgac
     1381 cgtaaaatga agttgatcga gatagcagag gccttaaaga tatcaaagga acgtgttggt
     1441 catatcattc atcaatattt ggatatgcgg aagctctgtg cgaaatgggt gccgcgcgag
     1501 ctcacatttg accaaaaaca acgacgtgtt gatgattcta agcggtgttt gcagctgtta
     1561 actcgtaata cacccgagtt tttccgtcga tatgtgacaa tggatgaaac atggctccat
     1621 cactacactc ctgagtccaa tcgacagtcg gctgagtgga cagcgaccgg tgaaccatct
     1681 ccgaagcgtg gaaagactca aaagtccgct ggcaaagtaa tggcctctgt tttttgggat
     1741 gcacatggaa taatttttat cgattatctt gagaagggaa aaaccatcaa cagtgactat
     1801 tatatggcgt tattggagcg tttgaaggtc gaaatcgcgg caaaacggcc ccacatgaag
     1861 aagaaaaaag tgttgttcca ccaagacaac gcaccgtgcc acaagtcatt gagaacgatg
     1921 gcaaaaattc atgaattggg cttcgaattg cttccccacc cgccgtattc tccagatctg
     1981 gcccccagcg actttttctt gttctcagac ctcaaaagga tgctcgcagg gaaaaaattt
     2041 ggctgcaatg aagaggtgat cgccgaaact gaggcctatt ttgaggcaaa accgaaggag
     2101 tactaccaaa atggtatcaa aaaattggaa ggtcgttata atcgttgtat cgctcttgaa
     2161 gggaactatg ttgaataagc ggccgccacc gcggtggagg ggaattccca tgtcagccgt
     2221 taagtgttcc tgtgtcactc aaaattgctt tgagaggctc taagggcttc tcagtgcgtt
     2281 acatccctgg cttgttgtcc acaaccgtta aaccttaaaa gctttaaaag ccttatatat
     2341 tctttttttt cttataaaac ttaaaacctt agaggctatt taagttgctg atttatatta
     2401 attttattgt tcaaacatga gagcttagta cgtgaaacat gagagcttag tacgttagcc
     2461 atgagagctt agtacgttag ccatgagggt ttagttcgtt aaacatgaga gcttagtacg
     2521 ttaaacatga gagcttagta cgtgaaacat gagagcttag tacgtactat caacaggttg
     2581 aactgctgat cttcagatcc tctacgccgg acgcatcgtg gccggatcaa ttccgtgata
     2641 ggtgggctgc ccttcctggt tggcttggtt tcatcagcca tccgcttgcc ctcatctgtt
     2701 acgccggcgg tagccggcca gcctcgcaga gcaggattcc cgttgagcac cgccaggtgc
     2761 gaataaggga cagtgaagaa ggaacacccg ctcgcgggtg ggcctacttc acctatcctg
     2821 cccggctgac gccgttggat acaccaagga aagtctacac gaaccctttg gcaaaatcct
     2881 gtatatcgtg cgaaaaagga tggatatacc gaaaaaatcg ctataatgac cccgaagcag
     2941 ggttatgcag cggaaaacgg aattgatccc cctcgaattg acgcgtttac caatgcttaa
     3001 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     3061 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     3121 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     3181 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     3241 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     3301 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     3361 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     3421 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     3481 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     3541 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     3601 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     3661 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     3721 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     3781 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     3841 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     3901 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     3961 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     4021 ataggcgtat cacgaggccc tttcgtcacg cgtcctcggt a
//
