LOCUS       sequence-462957-        4172 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Random transposon mutagenesis vector for Bacteroidales species - 
            erythromycin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4172)
  TITLE     A toolkit for transposon libraries and functional genomics in 
            intestinal Bacteroidales species
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCV006-ermg
FEATURES             Location/Qualifiers
     source          1..4172
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1080..1468
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            1583..1692
                     /note="incP origin of transfer"
     CDS             complement(join(1856..2647,2648..2716))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2479..2498
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2717..2821)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2889..2907
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(3977..4019)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 atggaaaaaa aggaatttcg tgttttgata aaatactgtt ttctgaaggg aaaaaataca
       61 gtggaagcaa aaacttggct tgataatgag tttccggact ctgccccagg gaaatcaaca
      121 ataattgatt ggtatgcaaa attcaagcgt ggtgaaatga gcacggagga cggtgaacgc
      181 agtggacgcc cgaaagaggt ggttaccgac gaaaacatca aaaaaatcca caaaatgatt
      241 ttgaatgacc gtaaaatgaa gttgatcgag atagcagagg ccttaaagat atcaaaggaa
      301 cgtgttggtc atatcattca tcaatatttg gatatgcgga agctctgtgc gaaatgggtg
      361 ccgcgcgagc tcacatttga ccaaaaacaa cgacgtgttg atgattctaa gcggtgtttg
      421 cagctgttaa ctcgtaatac acccgagttt ttccgtcgat atgtgacaat ggatgaaaca
      481 tggctccatc actacactcc tgagtccaat cgacagtcgg ctgagtggac agcgaccggt
      541 gaaccatctc cgaagcgtgg aaagactcaa aagtccgctg gcaaagtaat ggcctctgtt
      601 ttttgggatg cgcatggaat aatttttatc gattatcttg agaagggaaa aaccatcaac
      661 agtgactatt atatggcgtt attggagcgt ttgaaggtcg aaatcgcggc aaaacggccc
      721 cacatgaaga agaaaaaagt gttgttccac caagacaacg caccgtgcca caagtcattg
      781 agaacgatgg caaaaattca tgaattgggc ttcgaattgc ttccccaccc gccgtattct
      841 ccagatctgg cccccagcga ctttttcttg ttctcagacc tcaaaaggat gctcgcaggg
      901 aaaaaatttg gctgcaatga agaggtgatc gccgaaactg aggcctattt tgaggcaaaa
      961 ccgaaggagt actaccaaaa tggtatcaaa aaattggaag gtcgttataa tcgttgtatc
     1021 gctcttgaag ggaactatgt tgaataagcg gccgccaccg cggtggaggg gaattcccat
     1081 gtcagccgtt aagtgttcct gtgtcactca aaattgcttt gagaggctct aagggcttct
     1141 cagtgcgtta catccctggc ttgttgtcca caaccgttaa accttaaaag ctttaaaagc
     1201 cttatatatt cttttttttc ttataaaact taaaacctta gaggctattt aagttgctga
     1261 tttatattaa ttttattgtt caaacatgag agcttagtac gtgaaacatg agagcttagt
     1321 acgttagcca tgagagctta gtacgttagc catgagggtt tagttcgtta aacatgagag
     1381 cttagtacgt taaacatgag agcttagtac gtgaaacatg agagcttagt acgtactatc
     1441 aacaggttga actgctgatc ttcagatcct ctacgccgga cgcatcgtgg ccggatcaat
     1501 tccgtgatag gtgggctgcc cttcctggtt ggcttggttt catcagccat ccgcttgccc
     1561 tcatctgtta cgccggcggt agccggccag cctcgcagag caggattccc gttgagcacc
     1621 gccaggtgcg aataagggac agtgaagaag gaacacccgc tcgcgggtgg gcctacttca
     1681 cctatcctgc ccggctgacg ccgttggata caccaaggaa agtctacacg aaccctttgg
     1741 caaaatcctg tatatcgtgc gaaaaaggat ggatataccg aaaaaatcgc tataatgacc
     1801 ccgaagcagg gttatgcagc ggaaaacgga attgatcccc ctcgaattga cgcgtttacc
     1861 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     1921 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     1981 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     2041 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     2101 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     2161 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     2221 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     2281 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     2341 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     2401 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     2461 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     2521 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     2581 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     2641 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     2701 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     2761 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     2821 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     2881 cctataaaaa taggcgtatc acgaggccct ttcgtcacgc gtcctcggta cctaacaggt
     2941 tggatgataa gtccccggtc ttcgtagata aaacgaaagg ctcagtaaag agttaaggaa
     3001 agtgaagcat cttcgatgct ggagtgggat atactaaatt acctaaaaaa gtggcgcaca
     3061 aatttgcgcg ccacaattat tattcatacc tttgtggacc gtattacaaa gaacccaatc
     3121 atatgaacaa agtaaatata aaagatagtc aaaattttat tacttcaaaa tatcacatag
     3181 aaaaaataat gaattgcata agtttagatg aaaaagataa catctttgaa ataggtgcag
     3241 ggaaaggtca ttttactgct ggattggtaa agagatgtaa ttttgtaacg gcgatagaaa
     3301 ttgattctaa attatgtgag gtaactcgta ataagctctt aaattatcct aactatcaaa
     3361 tagtaaatga tgatatactg aaatttacat ttcctagcca caatccatat aaaatatttg
     3421 gcagcatacc ttacaacata agcacaaata taattcgaaa aattgttttt gaaagttcag
     3481 ccacaataag ttatttaata gtggaatatg gttttgctaa aatgttatta gatacaaaca
     3541 gatcactagc attgctgtta atggcagagg tagatatttc tatattagca aaaattccta
     3601 ggtattattt ccatccaaaa cctaaagtgg atagcacatt aattgtatta aaaagaaagc
     3661 cagcaaaaat ggcatttaaa gagagaaaaa aatatgaaac ttttgtaatg aaatgggtta
     3721 acaaagagta cgaaaaactg tttacaaaaa atcaatttaa taaagcttta aaacatgcga
     3781 gaatatatga tataaacaat attagtttcg aacaatttgt atcgctattt aatagttata
     3841 aaatatttaa cggctaactg cagggatgtc cacgaggtgt atgagacgtc agaattggtt
     3901 aattcgtctc tcagaattgg gagtctacga agaccgggga cttatcatcc aacctgttag
     3961 aaaggcgatg aaagagataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttac
     4021 aattgggcta cctttttttt gttttgtttg caatggttaa tctattgtta aaatttaaag
     4081 tttcacttga actttcaaat aatgttctta tatttgcagt gtcgaaagaa acaaagtagg
     4141 actgatcgtc catcaattta aaatttaaaa ta
//
