LOCUS       sequence-462963-       18499 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses VDE, PsbS, ZEP from A.thaliana.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18499)
  AUTHORS   Kromdijk J, Glowacka K, Leonelli L, Gabilly ST, Iwai M, Niyogi KK, 
            Long SP
  TITLE     Improving photosynthesis and crop productivity by accelerating 
            recovery from photoprotection.
  JOURNAL   Science. 2016 Nov 18;354(6314):857-861. doi: 
            10.1126/science.aai8878.
  PUBMED    27856901
REFERENCE   2  (bases 1 to 18499)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2016-11-18- 18"; volume: "354"; 
            issue: "6314"; pages: "857-861"
COMMENT     Sequence Label: pAtVPZv1.0
FEATURES             Location/Qualifiers
     source          1..18499
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(118..669)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(675..1055)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     protein_bind    1107..1131
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9570..9594)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9596..9622
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     terminator      9676..10383
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     primer_bind     complement(10408..10425)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10408..10424)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10417..10439)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    10627..10651
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             11952..12581
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             13010..14083
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      14149..14343
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(14579..14601)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    14687..14827
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     14742..14761
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(14842..14859)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15013..15601)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15093..15112)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(17026..17820)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    18245..18269
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(join(18355..18499,1..108))
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
ORIGIN
        1 tgtcttcaat tcagcccaaa tggcagttga aatactcaaa ccgccccata tgcaggagcg
       61 gatcattcat tgtttgtttg gttgcctttg ccaacatggg agtccaagat tctgcagtca
      121 aatctcggtg acgggcagga ccggacgggg cggtaccggc aggctgaagt ccagctgcca
      181 gaaacccacg tcatgccagt tcccgtgctt gaagccggcc gcccgcagca tgccgcgggg
      241 ggcatatccg agcgcctcgt gcatgcgcac gctcgggtcg ttgggcagcc cgatgacagc
      301 gaccacgctc ttgaagccct gtgcctccag ggacttcagc aggtgggtgt agagcgtgga
      361 gcccagtccc gtccgctggt ggcgggggga gacgtacacg gtcgactcgg ccgtccagtc
      421 gtaggcgttg cgtgccttcc aggggcccgc gtaggcgatg ccggcgacct cgccgtccac
      481 ctcggcgacg agccagggat agcgctcccg cagacggacg aggtcgtccg tccactcctg
      541 cggttcctgc ggctcggtac ggaagttgac cgtgcttgtc tcgatgtagt ggttgacgat
      601 ggtgcagacc gccggcatgt ccgcctcggt ggcacggcgg atgtcggccg ggcgtcgttc
      661 tgggctcatc gattcgattt ggtgtatcga gattggttat gaaattcaga tgctagtgta
      721 atgtattggt aatttgggaa gatataatag gaagcaaggc tatttatcca tttctgaaaa
      781 ggcgaaatgg cgtcaccgcg agcgtcacgc gcattccgtt cttgctgtaa agcgttgttt
      841 ggtacacttt tgactagcga ggcttggcgt gtcagcgtat ctattcaaaa gtcgttaatg
      901 gctgcggatc aagaaaaagt tggaatagaa acagaatacc cgcgaaattc aggcccggtt
      961 gccatgtcct acacgccgaa ataaacgacc aaattagtag aaaaataaaa actgactcgg
     1021 atacttacgt cacgtcttgc gcactgattt gaaaaatctc agaattgaat tctgcagtcg
     1081 acggtaccgc gggcccggga tcatcaacaa gtttgtacaa aaaagcaggc tccgcggccg
     1141 cccccttcac ccccgggaac attcatacgt tttcatttga aatatcctct ataattatat
     1201 tgaatttggc acataataag aaacctaatt ggtgatttat tttactagta aatttctggt
     1261 gatgggcttt ctactagaaa gctctcggaa aatcttggac caaatccata ttccatgact
     1321 tcgattgtta accctattag ttttcacaaa catactatca atatcattgc aacggaaaag
     1381 gtacaagtaa aacattcaat ccgataggga agtgatgtag gaggttggga agacaggccc
     1441 agaaagagat ttatctgact tgttttgtgt atagttttca atgttcataa aggaagatgg
     1501 agacttgaga agtttttttt ggactttgtt tagctttgtt gggcgttttt tttttttgat
     1561 caataacttt gttgggctta tgatttgtaa tattttcgtg gactctttag tttatttaga
     1621 cgtgctaact ttgttgggct tatgacttgt tgtaacatat tgtaacagat gacttgatgt
     1681 gcgactaatc tttacacatt aaacatagtt ctgttttttg aaagttctta ttttcatttt
     1741 tatttgaatg ttatatattt ttctatattt ataattctag taaaaggcaa attttgcttt
     1801 taaatgaaaa aaatatatat tccacagttt cacctaatct tatgcattta gcagtacaaa
     1861 ttcaaaaatt tcccattttt attcatgaat cataccatta tatattaact aaatccaagg
     1921 taaaaaaaag gtatgaaagc tctatagtaa gtaaaatata aattccccat aaggaaaggg
     1981 ccaagtccac caggcaagta aaatgagcaa gcaccactcc accatcacac aatttcactc
     2041 atagataacg ataagattca tggaattatc ttccacgtgg cattattcca gcggttcaag
     2101 ccgataaggg tctcaacacc tctccttagg cctttgtggc cgttaccaag taaaattaac
     2161 ctcacacata tccacactca aaatccaacg gtgtagatcc tagtccactt gaatctcatg
     2221 tatcctagac cctccgatca ctccaaagct tgttctcatt gttgttatca ttatatatag
     2281 atgaccaaag cactagacca aacctcagtc acacaaagag taaagaagaa caggtaccat
     2341 gggttcaact ccgttttgct actctatcaa tccatctcca tcaaagcttg atttcacgag
     2401 gacccatgtg tttagtcctg tttctaaaca gttttactta gatttatcat cgttttccgg
     2461 aaaacccgga ggagtatctg ggtttaggag ccgtcgagct ttgctcggag taaaggcggc
     2521 gacggcgtta gttgagaagg aggagaagag agaggcggtg acggagaaga agaagaaatc
     2581 gagggtttta gttgccggag gtggaatcgg aggattggtg tttgctttag cggctaagaa
     2641 gaaaggattc gatgtgttag tgtttgagaa agatttgagt gctataagag gagaaggaaa
     2701 atacagaggc ccgattcaaa tacagagcaa cgctttagct gctttggaag ctattgatat
     2761 tgaagttgct gaacaagtta tggaagctgg gtgtatcact ggtgatcgga ttaacggtct
     2821 cgttgatggt atctctggta cttggtatgt aaagtttgat actttcactc ctgcggcgtc
     2881 acggggactt cctgtgacta gagtaattag tagaatgact ctgcagcaga ttctagcacg
     2941 tgcggttgga gaagatgtga ttagaaacga gagtaatgtt gttgattttg aagattctgg
     3001 agataaggtt actgtggtac tcgagaatgg tcaacgctat gaaggtgatc tgcttgtggg
     3061 tgcagatggc atttggtcta aggtgagaaa taatttgttt ggccgtagtg aagctactta
     3121 ttcaggctac acttgttaca cggggattgc agattttata ccagcggata tcgagtctgt
     3181 tggctaccgg gttttcttgg gacacaaaca gtactttgtt tcttcggatg ttggtggtgg
     3241 aaaaatgcaa tggtatgcat ttcacgagga accagctggt ggggctgatg ctccaaatgg
     3301 tatgaagaaa aggttgtttg aaatatttga cggttggtgc gacaatgtac tcgacttgtt
     3361 gcatgcgact gaggaggaag ccattctgag aagagatatt tatgatagaa gtcctggttt
     3421 tacttggggt aaagggcgtg ttacgctgct cggggattct atccatgcga tgcagccaaa
     3481 tatgggtcaa ggtggatgca tggccattga ggatagtttt caactagcat tggagcttga
     3541 tgaagcatgg aaacagagtg ttgaaacgac tacacctgtt gatgttgttt cctctttgaa
     3601 aagatatgag gaatctagaa gactgagagt cgctattatc catgcaatgg cgaggatggc
     3661 tgcaattatg gcttccactt acaaagcata cttaggtgtt gggcttggtc ctctgtcttt
     3721 cttgacaaag tttagagtac cacatccagg aagagttggt ggtagattct tcgttgacat
     3781 tgctatgtca tcgatgcttg actgggtcct tggaggtaac agtgaaaaac tccaaggaag
     3841 gccacctagt tgcagactca ctgacaaagc cgatgaccgg cttcgagagt ggtttgaaga
     3901 tgacgatgct cttgaacgta ctataaaggg agaatggtat ctaattccac acggcgacga
     3961 ttgttgcgtt tcggaaacat tatgtctaac caaagatgaa gatcaacctt gcatcgtcgg
     4021 aagcgaacca gatcaagatt ttcctggaat gcgcattgtg atcccttcgt ctcaggtttc
     4081 gaagatgcat gctcgtgtga tttacaaaga cggagctttc ttcttgatgg atcttcgaag
     4141 cgaacacgga acctatgtga ccgataacga aggaagaaga tatagagcaa caccgaattt
     4201 tcccgcgcgg tttagatcgt ccgacatcat cgagtttggt tcagataaga aggcggcgtt
     4261 tagggtgaaa gtaatcagga aaactccgaa atcgacgagg aagaatgaga gtaacaacga
     4321 taaattactt cagacagctt gaggtaccat atgaagatga agatgaaata tttggtgtgt
     4381 caaataaaaa gcttgtgtgc ttaagtttgt gtttttttct tggcttgttg tgttatgaat
     4441 ttgtggcttt ttctaatatt aaatgaatgt aagatctcat tataatgaat aaacaaatgt
     4501 ttctataatc cattgtgaat gttttgttgg atctcttctg cagcatataa ctactgtatg
     4561 tgctatggta tggactatgg aacctgcagg catcaggcta gtgtcaatgc tatagcttgg
     4621 gctcctcata gctcttccca tatctgctcc gctggagatg attctcaggc gttgatttgg
     4681 gatatatctt ccatgggaca gcatgttgaa ggtggtctgg atccgattct agcttacaca
     4741 gccggcgctg aggttgagca gcttcagtgg tcatcttctc agcctgattg ggttgccatt
     4801 gccttctcta acaagctgca gattctccgg gtctgattat gttttacaga tttctgttgg
     4861 atacttggat gtaaaaactc gttgctattg ttgattaatg atcttttgat ctctgactct
     4921 aagcctcatt gagtttcctt tcatagtatg gtctgtgtag gaactatgta ccttcaaaat
     4981 tgtgaataac tagctttagc attggatttg attagacgta tggaagttag ttttgaacag
     5041 agagcatgtg acataacggg gaacttgaag tagattatga ctaacaccag catttatgtt
     5101 tctctgccct cgagctgatg gagttgcagg attttgtctg cctcaccatc gtatggaatg
     5161 tgtagctttc tatggtagtt gttataagtt ttgtctccat tgtccatatc actttatggg
     5221 tatgtagatt tgtgaaatta ggcatttgta acatcgtaca gatgatgtat tcttcattac
     5281 ttcaatacag gcatatagac tctctgtcca acttaaaagg catacaaggt ctgtggttta
     5341 ccaaaatcag ctttactaaa tcctttggcc aatattctct cagccagtcc taagaacctc
     5401 tgctctaaaa agtcttagct ttgaaagtca gacaaatgga gagcagcctt atcctccttt
     5461 tataaccgtg accacacaca caaatgaaga agcccaaatg aaaggcccaa aaatctaaaa
     5521 aataggtgaa ggagggactt tgtgaatatg tgatcataga aacatgaaga gacaagagag
     5581 tggtaaaacc acaaccatag aaacaagtaa gaaagctatc cagaatctaa tcttatttgc
     5641 ctccaagtca tcaattcttt atcctatatt actagtctct gcttcaccaa tcctttttgc
     5701 tctcctgtct gctacaaacc ttaacttgga ttctttctgg tcaccggtac catggctcaa
     5761 accatgctgc ttacttcagg cgtcaccgcc ggccattttt tgaggaacaa gagccctttg
     5821 gctcagccca aagttcacca tctcttcctc tctggaaact ctccggttgc actaccatct
     5881 aggagacaat cattcgttcc tctcgctctc ttcaaaccca aaaccaaagc tgctcctaaa
     5941 aaggttgaga agccgaagag caaggttgag gatggcatct ttggaacgtc tggtgggatt
     6001 ggtttcacaa aggcgaatga gctattcgtt ggtcgtgttg ctatgatcgg tttcgctgca
     6061 tcgttgcttg gtgaggcgtt gacgggaaaa gggatattag ctcagctgaa tctggagaca
     6121 gggataccga tttacgaagc agagccattg cttctcttct tcatcttgtt cactctgttg
     6181 ggagccattg gagctctcgg agacagagga aaattcgtcg acgatcctcc caccgggctc
     6241 gagaaagccg tcattcctcc cggcaaaaac gtccgatctg ccctcggtct caaagaacaa
     6301 ggtccattgt ttgggttcac gaaggcgaac gagttattcg taggaagatt ggcacagttg
     6361 ggaatagcat tttcactgat aggagagatt attaccggga aaggagcatt agctcaactc
     6421 aacattgaga ccggtatacc aattcaagat atcgaaccac ttgtcctctt aaacgttgct
     6481 ttcttcttct tcgctgccat taatcctggt aatggaaaat tcatcaccga tgatggtgaa
     6541 gaaagctaag gtaccatatg aagatgaaga tgaaatattt ggtgtgtcaa ataaaaagct
     6601 tgtgtgctta agtttgtgtt tttttcttgg cttgttgtgt tatgaatttg tggctttttc
     6661 taatattaaa tgaatgtaag atctcattat aatgaataaa caaatgtttc tataatccat
     6721 tgtgaatgtt ttgttggatc tcttctgcag catataacta ctgtatgtgc tatggtatgg
     6781 actatggaag aattccctac acatccactt ggccacctgt tgtgcaatga tgacgatgag
     6841 attgcagtgt ctctcgactg atgatgtaga cttcacaatc tcttaaaaat cccagtactt
     6901 aacctcagcc ttaagaaaac gcagggaact acgtcctcac aaaatctttc tctttgagtg
     6961 taactttcag acgcatcttt ggctctaaat tctaaaaagg aaaattttaa taggttttca
     7021 taatcatggg ttcactggat tagcatataa gtctatggtt gagaaacttg agacccagac
     7081 taacgaaacc tctttccgga tccaaaggtc ctcttgtgta gttgacgtgg taaactctct
     7141 accgtcaaat ttagacatta gctaatctga tcaataatct cggcagctct taaaaattaa
     7201 aaattagaaa tgatacgaac ctcataattt ttctttctct tatcaaaaca ccatctttgt
     7261 atctttataa gcctgttgcc actattttta attgaaaatg atgcgtttgt cttatgtttt
     7321 ctgtcctgga gttcaacatt atgacaatat gtatagtaaa ttagtgatat acaagacgtt
     7381 tgcaattcaa gaaaaaaact tataaactaa ttaatattat ggtccacggt gctacatatt
     7441 aactcttgat ggttttatac atcttttcta catgctaata tgcttttaat attgtagcct
     7501 aacgttataa tttgtttttt cttaaagaaa cagtatcttg aacgaatctt aactatttct
     7561 gtacttattc gattttattc gatttttatc cgtgtaaagg caaacgatta ttatgtaacg
     7621 acgggcataa aaagagtatc gatttcctat tcggagaaaa aaaaaaagat aaaaattgga
     7681 gtgtatgtat atttcttgaa ttgagagtaa tacaagatta cggtccaggt ggcggaatat
     7741 tattggcaag gtcacaagaa cctcaaataa ctgatctgaa gagaaatata aatccaaaag
     7801 agggaggagt gagagataag ggtggtgtca taagcgttta ctgtgagtct ctcaaagaaa
     7861 ccaaaggcag agaaaagaga taacacacac aaaaaaaagg taccatggca gtagctacac
     7921 attgtttcac ttcaccttgt catgaccgta ttcgattttt ctcaagtgat gatggtattg
     7981 gtaggcttgg cattacaaga aagaggatca atggcacttt cttgctcaag attttacctc
     8041 caatccaaag tgctgatctc agaacaactg gtgggagatc ctcacgtcct ttatctgcat
     8101 tcaggtcagg attctctaag gggatatttg acattgtgcc attaccatca aagaatgagc
     8161 tgaaagagct gaccgctccg ctgttgctaa aactcgtggg tgttttagct tgcgcgttcc
     8221 ttattgttcc atctgcagat gcagttgatg cacttaaaac ttgtgcatgc ttattgaagg
     8281 gatgcaggat agaactcgca aagtgcattg ccaaccctgc ctgtgcagcc aatgtcgcgt
     8341 gccttcagac ctgcaataac cgtccagatg aaaccgagtg ccagattaaa tgtggggatc
     8401 tgtttgagaa cagtgttgtt gatgagttca acgagtgtgc tgtgtcgaga aaaaagtgtg
     8461 ttcctagaaa atctgatctc ggagaatttc ctgccccaga cccttctgtt cttgtacaga
     8521 acttcaacat ctcggacttt aacgggaagt ggtacattac aagtggcttg aatccaacct
     8581 ttgatgcctt cgactgccag ctgcatgagt tccacacaga aggtgacaac aagcttgttg
     8641 gaaacatctc ttggagaata aagaccctag acagtggatt ctttactagg tcagccgtac
     8701 aaaaattcgt gcaagatcct aaccaacctg gtgttctcta caatcatgac aacgagtacc
     8761 ttcactatca agatgactgg tatatcctgt catcaaagat agagaataaa cctgaagact
     8821 atatatttgt atactaccgt gggcgaaacg atgcttggga tggatatggt ggtgcagttg
     8881 tatacacgag aagttctgta ttacccaata gcattatacc agaactcgaa aaagcagcaa
     8941 aaagcatagg cagagacttc agcacattca ttagaacgga taacacatgt ggtcctgaac
     9001 ctgcgctcgt ggagagaatt gagaagacag tggaagaagg tgaaaggata atcgtaaaag
     9061 aggttgaaga gatagaagaa gaggtagaga aggaagtgga gaaggtcggt aggactgaga
     9121 tgaccttgtt ccagagattg gctgaaggat ttaatgaact gaagcaagac gaggagaatt
     9181 tcgtgagaga gttaagtaaa gaagagatgg agtttttgga tgagatcaaa atggaagcaa
     9241 gtgaggttga aaaattgttt gggaaagctt tgccaatcag gaaggtcagg tagggtacca
     9301 tatgaagatg aagatgaaat atttggtgtg tcaaataaaa agcttgtgtg cttaagtttg
     9361 tgtttttttc ttggcttgtt gtgttatgaa tttgtggctt tttctaatat taaatgaatg
     9421 taagatctca ttataatgaa taaacaaatg tttctataat ccattgtgaa tgttttgttg
     9481 gatctcttct gcagcatata actactgtat gtgctatggt atggactatg gaagaattcc
     9541 ctgcaggccc gggaagggtg ggcgcgccga cccagctttc ttgtacaaag tggtgtaccc
     9601 atacgatgtt ccagattacg cttgactagg tgagtctaga gagttaatta agacccggga
     9661 ctagtcccta gagtcctgct ttaatgagat atgcgagacg cctatgatcg catgatattt
     9721 gctttcaatt ctgttgtgca cgttgtaaaa aacctgagca tgtgtagctc agatccttac
     9781 cgccggtttc ggttcattct aatgaatata tcacccgtta ctatcgtatt tttatgaata
     9841 atattctccg ttcaatttac tgattgtacc ctactactta tatgtacaat attaaaatga
     9901 aaacaatata ttgtgctgaa taggtttata gcgacatcta tgatagagcg ccacaataac
     9961 aaacaattgc gttttattat tacaaatcca attttaaaaa aagcggcaga accggtcaaa
    10021 cctaaaagac tgattacata aatcttattc aaatttcaaa agtgccccag gggctagtat
    10081 ctacgacaca ccgagcggcg aactaataac gctcactgaa gggaactccg gttccccgcc
    10141 ggcgcgcatg ggtgagattc cttgaagttg agtattggcc gtccgctcta ccgaaagtta
    10201 cgggcaccat tcaacccggt ccagcacggc ggccgggtaa ccgacttgct gccccgagaa
    10261 ttatgcagca tttttttggt gtatgtgggc cccaaatgaa gtgcaggtca aaccttgaca
    10321 gtgacgacaa atcgttgggc gggtccaggg cgaattttgc gacaacatgt cgaggctcag
    10381 caggacctgc aggcatgcaa gcttggcact ggccgtcgtt ttacaacgtc gtgactggga
    10441 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
    10501 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
    10561 atgctagagc agcttgagct tggatcagat tgtcgtttcc cgccttcagt ttaaactatc
    10621 agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa
    10681 tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca
    10741 accacagggt tcccctcggg atcaaagtac tttgatccaa cccctccgct gctatagtgc
    10801 agtcggcttc tgacgttcag tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta
    10861 agttacgcga caggctgccg ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag
    10921 tcgcataaag tagaatactt gcgactagaa ccggagacat tacgccatga acaagagcgc
    10981 cgccgctggc ctgctgggct atgcccgcgt cagcaccgac gaccaggact tgaccaacca
    11041 acgggccgaa ctgcacgcgg ccggctgcac caagctgttt tccgagaaga tcaccggcac
    11101 caggcgcgac cgcccggagc tggccaggat gcttgaccac ctacgccctg gcgacgttgt
    11161 gacagtgacc aggctagacc gcctggcccg cagcacccgc gacctactgg acattgccga
    11221 gcgcatccag gaggccggcg cgggcctgcg tagcctggca gagccgtggg ccgacaccac
    11281 cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc attgccgagt tcgagcgttc
    11341 cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt
    11401 tggcccccgc cctaccctca ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca
    11461 ggaaggccgc accgtgaaag aggcggctgc actgcttggc gtgcatcgct cgaccctgta
    11521 ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag gccaggcggc gcggtgcctt
    11581 ccgtgaggac gcattgaccg aggccgacgc cctggcggcc gccgagaatg aacgccaaga
    11641 ggaacaagca tgaaaccgca ccaggacggc caggacgaac cgtttttcat taccgaagag
    11701 atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc
    11761 gtgcggctgc atgaaatcct ggccggtttg tctgatgcca agctggcggc ctggccggcc
    11821 agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa
    11881 aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa acaaatacgc
    11941 aaggggaacg catgaaggtt atcgctgtac ttaaccagaa aggcgggtca ggcaagacga
    12001 ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg
    12061 attccgatcc ccagggcagt gcccgcgatt gggcggccgt gcgggaagat caaccgctaa
    12121 ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt gaaggccatc ggccggcgcg
    12181 acttcgtagt gatcgacgga gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg
    12241 cagccgactt cgtgctgatt ccggtgcagc caagccctta cgacatatgg gccaccgccg
    12301 acctggtgga gctggttaag cagcgcattg aggtcacgga tggaaggcta caagcggcct
    12361 ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg
    12421 ccgggtacga gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc tacccaggca
    12481 ctgccgccgc cggcacaacc gttcttgaat cagaacccga gggcgacgct gcccgcgagg
    12541 tccaggcgct ggccgctgaa attaaatcaa aactcatttg agttaatgag gtaaagagaa
    12601 aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc
    12661 tgcaacgttg gccagcctgg cagacacgcc agccatgaag cgggtcaact ttcagttgcc
    12721 ggcggaggat cacaccaagc tgaagatgta cgcggtacgc caaggcaaga ccattaccga
    12781 gctgctatct gaatacatcg cgcagctacc agagtaaatg agcaaatgaa taaatgagta
    12841 gatgaatttt agcggctaaa ggaggcggca tggaaaatca agaacaacca ggcaccgacg
    12901 ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg
    12961 cctgccggcc ctgcaatggc actggaaccc ccaagcccga ggaatcggcg tgagcggtcg
    13021 caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt
    13081 gaaggccgcg caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc
    13141 gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc
    13201 gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct
    13261 ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc
    13321 gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga
    13381 ggtttccgca gggccggccg gcatggccag tgtgtgggat tacgacctgg tactgatggc
    13441 ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag acaagcccgg
    13501 ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag ccgatggcgg
    13561 aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc acgttgccat
    13621 gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg gtgaagcctt
    13681 gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca tcgagatcga
    13741 gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg tgctgacggt
    13801 tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc gcctggcacg
    13861 ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg
    13921 cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga
    13981 cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg
    14041 ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct
    14101 agggcaaatt gccctagcag gggaaaaagg tcgaaaaggt ctctttcctg tggatagcac
    14161 gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa
    14221 gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga
    14281 tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc
    14341 ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct
    14401 gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat
    14461 ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt cgccactcga
    14521 ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc
    14581 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
    14641 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc
    14701 agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt
    14761 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
    14821 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
    14881 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
    14941 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
    15001 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
    15061 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
    15121 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
    15181 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
    15241 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
    15301 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
    15361 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
    15421 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
    15481 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
    15541 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
    15601 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
    15661 agggctgatg aatcccctaa tgatttttat caaaatcatt aagttaaggt agatacacat
    15721 cttgtcatat gatcaaatgg tttcgccaaa aatcaataat cagacaacaa aatgtgcgaa
    15781 ctcgatattt tacacgactc tctttaccaa ttctgccccg aattacactt aaaacgactc
    15841 aacagcttaa cgttggcttg ccacgcctta cttgactgta aaactctcac tcttaccgaa
    15901 cttggccgta acctgccaac caaagcgaga acaaaacata acatcaaacg aatcgaccga
    15961 ttgttaggta atcgtcacct ccacaaagag cgactcgctg tataccgttg gcatgctagc
    16021 tttatctgtt cgggcaatac gatgcccatt gtacttgttg actggtctga tatccgtgag
    16081 caaaaacggc ttatggtatt gcgagcttca gtcgcactac acggtcgttc tgttactctt
    16141 tatgagaaag cgttcccgct ttcagagcaa tgttcaaaga aagctcatga ccaatttcta
    16201 gccgaccttg cgagcattct accgagtaac accacaccgc tcattgtcag tgatgctggc
    16261 tttaaagtgc catggtataa atccgttgag aagctgggtt ggtactggtt aagtcgagta
    16321 agaggaaaag tacaatatgc agacctagga gcggaaaact ggaaacctat cagcaactta
    16381 catgatatgt catctagtca ctcaaagact ttaggctata agaggctgac taaaagcaat
    16441 ccaatctcat gccaaattct attgtataaa tctcgctcta aaggccgaaa aaatcagcgc
    16501 tcgacacgga ctcattgtca ccacccgtca cctaaaatct actcagcgtc ggcaaaggag
    16561 ccatggattc tagcaactaa cttacctgtt gaaattcgaa cacccaaaca acttgttaat
    16621 atctattcga agcgaatgca gattgaagaa accttccgag acttgaaaag tcctgcctac
    16681 ggactaggcc tacgccatag ccgaacgagc agctcagagc gttttgatat catgctgcta
    16741 atcgccctga tgcttcaact aacatgttgg cttgcgggcg ttcatgctca gaaacaaggt
    16801 tgggacaagc acttccaggc taacacagtc agaaatcgaa acgtactctc aacagttcgc
    16861 ttaggcatgg aagttttgcg gcattctggc tacacaataa caagggaaga cttactcgtg
    16921 gctgcaaccc tactagctca aaatttattc acacatggtt acgctttggg gaaattatga
    16981 ggggatctct cagcgttaag ggattttggt catgcattct aggtactaaa acaattcatc
    17041 cagtaaaata taatatttta ttttctccca atcaggcttg atccccagta agtcaaaaaa
    17101 tagctcgaca tactgttctt ccccgatatc ctccctgatc gaccggacgc agaaggcaat
    17161 gtcataccac ttgtccgccc tgccgcttct cccaagatca ataaagccac ttactttgcc
    17221 atctttcaca aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc
    17281 gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc
    17341 ttcccagttt tcgcaatcca catcggccag atcgttattc agtaagtaat ccaattcggc
    17401 taagcggctg tctaagctat tcgtataggg acaatccgat atgtcgatgg agtgaaagag
    17461 cctgatgcac tccgcataca gctcgataat cttttcaggg ctttgttcat cttcatactc
    17521 ttccgagcaa aggacgccat cggcctcact catgagcaga ttgctccagc catcatgccg
    17581 ttcaaagtgc aggacctttg gaacaggcag ctttccttcc agccatagca tcatgtcctt
    17641 ttcccgttcc acatcatagg tggtcccttt ataccggctg tccgtcattt ttaaatatag
    17701 gttttcattt tctcccacca gcttatatac cttagcagga gacattcctt ccgtatcttt
    17761 tacgcagcgg tatttttcga tcagtttttt caattccggt gatattctca ttttagccat
    17821 ttattatttc cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac
    17881 aagacgaact ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct
    17941 ttttcaaagt tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa
    18001 ccgcggtgat cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc
    18061 gagatcatcc gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta
    18121 acatgagcaa agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg
    18181 ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg
    18241 ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat
    18301 tgcggacgtt tttaatgtac tgaattaacg ccgaattaat tcgagctcgg atctgataat
    18361 ttatttgaaa attcataaga aaagcaaacg ttacatgaat tgatgaaaca atacaaagac
    18421 agataaagcc acgcacattt aggatattgg ccgagattac tgaatattga gtaagatcac
    18481 ggaatttctg acaggagca
//
