LOCUS       sequence-462965-        3045 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pENTR4 plasmid with CDS of Pseudomonas syringae pv tomato type III 
            effector HopU1 without stop codon for C-terminal epitope tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Simranjit Kaur, Thomas Colby, Domenika Thieme, Carsten Proksch, 
            Susanne Matschi, eIvan Matic,, Lennart Wirthmueller
  TITLE     Untargeted proteomics identifies plant substrates of the 
            bacterial-derived ADP-ribosyltransferase AvrRpm1
  JOURNAL   bioRxiv 2023.09.25.558804
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.09.25.558804"
COMMENT     Sequence Label: pENTR4 HopU1
FEATURES             Location/Qualifiers
     source          1..3045
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(63..82)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             74..883
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPGDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(141..160)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      973..1561
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1462..1481
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      1726..1812
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1904..1931
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     1939..1962
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    1981..2080
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2897..2996)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa
       61 tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa
      121 catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc
      181 gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa
      241 aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt
      301 tatgcctctt ccgaccatca agcattttat ccgtactcct ggtgatgcat ggttactcac
      361 cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga
      421 aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa
      481 ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa
      541 cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt
      601 ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga
      661 tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg
      721 acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga
      781 gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat
      841 gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg
      901 gttgtaacat tattcagatt gggccccgtt ccactgagcg tcagaccccg tagaaaagat
      961 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     1021 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     1081 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     1141 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     1201 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     1261 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     1321 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     1381 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     1441 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     1501 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     1561 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     1621 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcta gcatggatct
     1681 cggggacgtc taactactaa gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag
     1741 gctcagtcgg aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
     1801 agtaggacaa atccgccggg agcggatttg aacgttgtga agcaacggcc cggagggtgg
     1861 cgggcaggac gcccgccata aactgccagg catcaaacta agcagaaggc catcctgacg
     1921 gatggccttt ttgcgtttct acaaactctt cctgttagtt agttacttaa gctcgggccc
     1981 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgataagcaa
     2041 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggc aaatataaat
     2101 cgacaactgc ctgtatcagg ctcggagcga ttgttgactc ccgacgtggg cgtatctcgc
     2161 caggcttgtt ccgaaaggca ttattctact ggacaggatc ggcatgattt ttaccgtttt
     2221 gctgccaggc tacatgtgga tgcgcagtgt tttggtctgt caatagacga tttgatggat
     2281 aagttttctg acaagcactt cagggctgag catcctgaat acagggatgt ctatccggag
     2341 gaatgttctg ccatttatat gcataccgct caagactatt ctagtcacct cgtaaggggg
     2401 gaaataggaa cgccgctgta ccgagaggtc aataattatc ttcgacttca acatgagaat
     2461 tctgggcgag aagctgaaat tgataatcac gacgaaaagc tatcgcctca cataaaaatg
     2521 ctttcatctg cgcttaatcg tttaatggat gtcgccgctt ttagaggaac ggtttataga
     2581 ggcattcgcg gtgatttaga taccattgct cggctctacc atctattcga tacgggcggc
     2641 cggtacgtag agcccgcttt catgagtaca actcgaataa aggacagtgc ccaggtgttt
     2701 gagccaggca cgccaaacaa catagctttc cagataagcc taaaaagagg cgccgacatt
     2761 tcgggatctt cccaagcgcc ctcagaggaa gaaatcatgc tacccatgat gagtgagttc
     2821 gtcattgaac atgcatccgc tctttccgaa ggaaagcatt tatttgtatt aagtcagatt
     2881 aactcgagat atctagaccc agctttcttg tacaaagttg gcattataag aaagcattgc
     2941 ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat
     3001 ccagctgcag ctctggcccg tgtctcaaaa tctctgatgt tacat
//
