LOCUS       sequence-462972-       10235 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vinculin FRET tension sensor with coiled-coil Mad domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10235)
  AUTHORS   Liu S, Liu J, Foote A, Ogasawara H, Al Abdullatif S, Batista VS, 
            Salaita K
  TITLE     Digital and Tunable Genetically Encoded Tension Sensors Based on 
            Engineered Coiled-Coils.
  JOURNAL   Angew Chem Int Ed Engl. 2025 Jan 13:e202407359. doi: 
            10.1002/anie.202407359.
  PUBMED    39805005
REFERENCE   2  (bases 1 to 10235)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2025 Jan 13:e202407359. doi: 10.1002/anie.202407359."
COMMENT     Sequence Label: Vh-Clover-CC-Mad-mRuby2-Vt
FEATURES             Location/Qualifiers
     source          1..10235
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3623..3644)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     4231..4252
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5826..5843)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5832..6056
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6102..6530
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6189..6208)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6399..6420
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6539..6559)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6544..6873
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6724..6859
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6786..6805
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6940..7734
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6994..7013)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7604..7623
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7908..8029
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7945..7964)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7999..8018
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8078..8094)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8078..8094)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8091..8113)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8102..8118
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8126..8132,8133..8150,8151..8156))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8171..8192
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8309..8326)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8480..9068)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8560..8579)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9239..10030,10031..10099))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9862..9881
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10100..10204)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttgcca tgcccgtctt ccacacgcgc accatcgaga gcatcttgga
      961 gcccgtggct cagcagatct cccacctggt catcatgcac gaggaggggg aggtagacgg
     1021 caaggccatc ccggacctca ccgcccccgt gtcggccgtg caggccgctg tcagcaacct
     1081 ggtgcgggtt ggaaaagaaa ctgtgcagac aacagaagac cagatcttga aaagggatat
     1141 gccaccagca ttcatcaaag tagagaatgc ctgcaccaag ctcgttcgag cagcccagat
     1201 gctgcaagca gatccttatt cagtaccagc tcgtgactac ctaattgatg gatcaagagg
     1261 catcctttct ggaacatcag acttacttct gacatttgat gaagcggagg tccgtaaaat
     1321 catccgtgtc tgcaaaggaa tattggaata tctgactgtg gcagaagtag tagagactat
     1381 ggaggatttg gtgacatata caaagaatct agggccagga atgacaaaga tggcgaaaat
     1441 gatcgatgag agacaacagg aattaactca tcaggaacat agggttatgc tggtgaactc
     1501 catgaatact gtgaaggagc tattgcctgt acttatttca gctatgaaga tctttgtaac
     1561 cacaaaaaac actaaaagcc agggaataga agaggccttg aaaaatcgca atttcacagt
     1621 agagaaaatg agtgctgaga taaatgaaat aatccgtgta ttacaactca cttcctggga
     1681 tgaagatgcc tgggccagca aggacactga agccatgaaa agagctttag ccctaataga
     1741 ttcaaagatg aaccaggcaa aaggctggct gagagatcca aacgcacctc caggggatgc
     1801 tggtgagcaa gcaataaggc agatccttga tgaagctgga aaagcaggag aattgtgtgc
     1861 aggcaaagaa cgcagggaga ttctgggaac atgcaaaact ttgggccaaa tgactgatca
     1921 acttgctgat ctccgagcta gaggacaggg tgctacaccg atggcaatgc agaaggcaca
     1981 gcaggtgtca caaggcctgg atttgctcac tgcaaaagtg gagaatgcag cccggaaatt
     2041 ggaggccatg acaaactcta agcaggctat tgcaaagaag attgatgctg ctcagaactg
     2101 gcttgcggat cccaacggcg gaagtgaagg agaagaacac attcgaggaa ttatgtctga
     2161 agcaaggaaa gttgcagaat tgtgtgagga gcctaaagaa agagatgata tccttcgctc
     2221 cttgggggaa atctctgctc tgacagctaa gctgtcagat ctgcgacgac atgggaaagg
     2281 cgactctcct gaggcccgtg cattggccaa gcaaatagct acatcacttc agaacttaca
     2341 gtccaaaaca aacagagctg tagcaaatac taggccagtt aaagctgctg tccatttgga
     2401 gggcaagatt gagcaagccc agaggtggat agataatcct acagttgatg atcggggagt
     2461 aggccaggca gcaattcggg gtttggttgc agaaggtcgt cgtctagcca atgtcatgat
     2521 gggaccttat cgtcaggacc tgcttgccaa atgtgaccgt gtagaccagc tggctgctca
     2581 gcttgctgac cttgcagcaa gaggggaagg agagtctcct caggctaggg caattgctgc
     2641 tcagcttcag gactccctga aggatctcaa agcacggatg caagaagcaa tgacccagga
     2701 ggtgtctgat gttttcagtg acactacaac ccctattaag ttgttagcag tagcagccac
     2761 tgctccttct gatactccca atagagaaga ggtgtttgaa gaaagagcag caaattttga
     2821 aaaccatgct gctagactgg gagcaacagc agaaaaagca gctgcagttg gaactgctaa
     2881 taaaactact gtggaaggca ttcaggcaac agtcaaatct gcaagggagc ttacaccaca
     2941 ggtagtatcg gctgctcgaa tcctcctgag aaatcctgga aatcaagctg cttatgagca
     3001 ttttgagaca atgaaaaacc aatggattga taatgtagaa aagatgacag ggctggtgga
     3061 tgaggccatc gataccaagt ctctgttgga tgcatcagaa gaggctatta agaaggatct
     3121 tgataaatgt aaagttgcaa tggccaatat gcaacctcag atgctggtag ctggagccac
     3181 cagcattgct agacgagcga accgcatcct gcttgtggca aaacgggagg ttgaaaattc
     3241 agaagaccct aaattcaggg aggctgttaa agcagcttct gatgagctga gcaaaaccat
     3301 atcaccgatg gtaatggatg ctaaagctgt agcaggaaat atctctgatc ctggtttgca
     3361 gaagagtttc ttggattctg gatacaggat tctgggagct gtggccaaag tcagagaagc
     3421 ctttcagcct caggagccag actttccccc tcctcctcct gacctagagc atctccatct
     3481 gactgatgag cttgctcctc caaaaccacc acttccagaa ggtgaggttc ccccacccag
     3541 accaccaccc cctgaagaaa aagatgaggt cgagatgatg gtgagcaagg gcgaggagct
     3601 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     3661 cagcgtccgc ggcgagggcg agggcgatgc caccaacggc aagctgaccc tgaagttcat
     3721 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg
     3781 cgtggcctgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     3841 catgcccgaa ggctacgtcc aggagcgcac catctctttc aaggacgacg gtacctacaa
     3901 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     3961 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca acttcaacag
     4021 ccacaacgtc tatatcacgg ccgacaagca gaagaacggc atcaaggcta acttcaagat
     4081 ccgccacaac gttgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     4141 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagccatc agtccgccct
     4201 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     4261 cgggtccgga gggggcagtg cccaacttga aaaagagatg caggcacttg aaaaggagaa
     4321 cgcacagttg gagtgggaaa tgcaggcttt ggagaaggag atggctcagg gtgggagtgg
     4381 aggcagtcag gccatgaaaa agaagctcgc tcaaatgaag tggaagctgc aagctaataa
     4441 aaaaaagctg gcccagatga aaaagaaact tcaggccggg ggtagcaccg gtaaaggtga
     4501 agaattaatc aaggaaaata tgcgtatgaa ggtggtcatg gaaggttcgg tcaacggcca
     4561 ccaattcaaa tgcacaggtg aaggagaagg caatccgtac atgggaactc aaaccatgag
     4621 gatcaaagtc atcgagggag gacccctgcc atttgccttt gacattcttg ccacgtcgtt
     4681 catgtatggc agccgtactt ttatcaagta cccgaaaggc attcctgatt tctttaaaca
     4741 gtcctttcct gagggtttta cttgggaaag agttacgaga tacgaagatg gtggagtcgt
     4801 caccgtcatg caggacacca gccttgagga tggctgtctc gtttaccacg tccaagtcag
     4861 aggggtaaac tttccctcca atggtcccgt gatgcagaag aagaccaagg gttgggagcc
     4921 taatacagag atgatgtatc cagcagatgg tggtctgagg ggatacactc atatggcact
     4981 gaaagttgat ggtggtggcc atctgtcttg ctctttcgta acaacttaca ggtcaaaaaa
     5041 gaccgtcggg aacatcaaga tgcccggtat ccatgccgtt gatcaccgcc tggaaaggtt
     5101 agaggaaagt gacaatgaaa tgttcgtagt acaacgcgaa cacgcagttg ccaagttcgc
     5161 cgggcttggt ggtgggatgg acgagctgta caaggcggcc gcggagttcc cagagcagaa
     5221 agcaggagaa gctattaatc agcccatgat gatggctgct aggcagttgc atgacgaggc
     5281 tcggaaatgg tctagcaagg gtaacgacat cattgctgct gctaaacgaa tggcgctgct
     5341 catggctgag atgtcacgcc tggtgcgagg aggcagcgga aacaagcgtg ccctcatcca
     5401 gtgtgcaaaa gatattgcta aggcatcgga tgaagtcact cgattggcca aagaggtggc
     5461 caagcaatgt actgataaac gcattagaac aaacctctta caggtctgtg agcgaatccc
     5521 aaccatcagc acgcagctca aaattctttc cacagtgaaa gctaccatgc tgggcaggac
     5581 taacatcagc gatgaagaat cagaacaggc aactgagatg ttggttcata acgcccagaa
     5641 cctcatgcag tctgtgaagg aaactgtgag agaagctgaa gcagcatcca ttaagataag
     5701 aacagatgcc ggattcactc tgcgctgggt cagaaagacc ccatggtatc agtaagaatt
     5761 ctgcagatat ccagcacagt ggcggccgct cgagtctaga gggcccgttt aaacccgctg
     5821 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     5881 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     5941 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     6001 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     6061 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     6121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     6181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     6241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     6301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     6361 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     6421 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     6481 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
     6541 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     6601 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     6661 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     6721 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     6781 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
     6841 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
     6901 ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
     6961 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     7021 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     7081 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
     7141 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     7201 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     7261 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     7321 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     7381 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     7441 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc
     7501 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     7561 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     7621 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     7681 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
     7741 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt
     7801 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     7861 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
     7921 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     7981 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
     8041 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     8101 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     8161 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     8221 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     8281 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     8341 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     8401 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     8461 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     8521 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     8581 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     8641 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     8701 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     8761 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     8821 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     8881 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     8941 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     9001 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     9061 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     9121 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     9181 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     9241 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     9301 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     9361 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     9421 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     9481 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     9541 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     9601 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     9661 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     9721 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     9781 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     9841 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     9901 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     9961 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    10021 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    10081 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    10141 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    10201 cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
