LOCUS       sequence-462980-        9626 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Parallel coiled-coil with (GGS)12 linker to prevent condensation  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9626)
  AUTHORS   Liu S, Liu J, Foote A, Ogasawara H, Al Abdullatif S, Batista VS, 
            Salaita K
  TITLE     Digital and Tunable Genetically Encoded Tension Sensors Based on 
            Engineered Coiled-Coils.
  JOURNAL   Angew Chem Int Ed Engl. 2025 Jan 13:e202407359. doi: 
            10.1002/anie.202407359.
  PUBMED    39805005
REFERENCE   2  (bases 1 to 9626)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2025 Jan 13:e202407359. doi: 10.1002/anie.202407359."
COMMENT     Sequence Label: pcDNA3.1-Vh-clover-GCN4-(GGS)12-Vt
FEATURES             Location/Qualifiers
     source          1..9626
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3623..3644)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     4231..4252
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5217..5234)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5223..5447
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5493..5921
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5580..5599)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5790..5811
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5930..5950)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5935..6264
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6115..6250
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6177..6196
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6331..7125
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6385..6404)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6995..7014
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7299..7420
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7336..7355)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7390..7409
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7469..7485)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7469..7485)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7482..7504)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7493..7509
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7517..7523,7524..7541,7542..7547))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7562..7583
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7700..7717)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7871..8459)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7951..7970)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8630..9421,9422..9490))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9253..9272
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9491..9595)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttgcca tgcccgtctt ccacacgcgc accatcgaga gcatcttgga
      961 gcccgtggct cagcagatct cccacctggt catcatgcac gaggaggggg aggtagacgg
     1021 caaggccatc ccggacctca ccgcccccgt gtcggccgtg caggccgctg tcagcaacct
     1081 ggtgcgggtt ggaaaagaaa ctgtgcagac aacagaagac cagatcttga aaagggatat
     1141 gccaccagca ttcatcaaag tagagaatgc ctgcaccaag ctcgttcgag cagcccagat
     1201 gctgcaagca gatccttatt cagtaccagc tcgtgactac ctaattgatg gatcaagagg
     1261 catcctttct ggaacatcag acttacttct gacatttgat gaagcggagg tccgtaaaat
     1321 catccgtgtc tgcaaaggaa tattggaata tctgactgtg gcagaagtag tagagactat
     1381 ggaggatttg gtgacatata caaagaatct agggccagga atgacaaaga tggcgaaaat
     1441 gatcgatgag agacaacagg aattaactca tcaggaacat agggttatgc tggtgaactc
     1501 catgaatact gtgaaggagc tattgcctgt acttatttca gctatgaaga tctttgtaac
     1561 cacaaaaaac actaaaagcc agggaataga agaggccttg aaaaatcgca atttcacagt
     1621 agagaaaatg agtgctgaga taaatgaaat aatccgtgta ttacaactca cttcctggga
     1681 tgaagatgcc tgggccagca aggacactga agccatgaaa agagctttag ccctaataga
     1741 ttcaaagatg aaccaggcaa aaggctggct gagagatcca aacgcacctc caggggatgc
     1801 tggtgagcaa gcaataaggc agatccttga tgaagctgga aaagcaggag aattgtgtgc
     1861 aggcaaagaa cgcagggaga ttctgggaac atgcaaaact ttgggccaaa tgactgatca
     1921 acttgctgat ctccgagcta gaggacaggg tgctacaccg atggcaatgc agaaggcaca
     1981 gcaggtgtca caaggcctgg atttgctcac tgcaaaagtg gagaatgcag cccggaaatt
     2041 ggaggccatg acaaactcta agcaggctat tgcaaagaag attgatgctg ctcagaactg
     2101 gcttgcggat cccaacggcg gaagtgaagg agaagaacac attcgaggaa ttatgtctga
     2161 agcaaggaaa gttgcagaat tgtgtgagga gcctaaagaa agagatgata tccttcgctc
     2221 cttgggggaa atctctgctc tgacagctaa gctgtcagat ctgcgacgac atgggaaagg
     2281 cgactctcct gaggcccgtg cattggccaa gcaaatagct acatcacttc agaacttaca
     2341 gtccaaaaca aacagagctg tagcaaatac taggccagtt aaagctgctg tccatttgga
     2401 gggcaagatt gagcaagccc agaggtggat agataatcct acagttgatg atcggggagt
     2461 aggccaggca gcaattcggg gtttggttgc agaaggtcgt cgtctagcca atgtcatgat
     2521 gggaccttat cgtcaggacc tgcttgccaa atgtgaccgt gtagaccagc tggctgctca
     2581 gcttgctgac cttgcagcaa gaggggaagg agagtctcct caggctaggg caattgctgc
     2641 tcagcttcag gactccctga aggatctcaa agcacggatg caagaagcaa tgacccagga
     2701 ggtgtctgat gttttcagtg acactacaac ccctattaag ttgttagcag tagcagccac
     2761 tgctccttct gatactccca atagagaaga ggtgtttgaa gaaagagcag caaattttga
     2821 aaaccatgct gctagactgg gagcaacagc agaaaaagca gctgcagttg gaactgctaa
     2881 taaaactact gtggaaggca ttcaggcaac agtcaaatct gcaagggagc ttacaccaca
     2941 ggtagtatcg gctgctcgaa tcctcctgag aaatcctgga aatcaagctg cttatgagca
     3001 ttttgagaca atgaaaaacc aatggattga taatgtagaa aagatgacag ggctggtgga
     3061 tgaggccatc gataccaagt ctctgttgga tgcatcagaa gaggctatta agaaggatct
     3121 tgataaatgt aaagttgcaa tggccaatat gcaacctcag atgctggtag ctggagccac
     3181 cagcattgct agacgagcga accgcatcct gcttgtggca aaacgggagg ttgaaaattc
     3241 agaagaccct aaattcaggg aggctgttaa agcagcttct gatgagctga gcaaaaccat
     3301 atcaccgatg gtaatggatg ctaaagctgt agcaggaaat atctctgatc ctggtttgca
     3361 gaagagtttc ttggattctg gatacaggat tctgggagct gtggccaaag tcagagaagc
     3421 ctttcagcct caggagccag actttccccc tcctcctcct gacctagagc atctccatct
     3481 gactgatgag cttgctcctc caaaaccacc acttccagaa ggtgaggttc ccccacccag
     3541 accaccaccc cctgaagaaa aagatgaggt cgagatgatg gtgagcaagg gcgaggagct
     3601 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     3661 cagcgtccgc ggcgagggcg agggcgatgc caccaacggc aagctgaccc tgaagttcat
     3721 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg
     3781 cgtggcctgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     3841 catgcccgaa ggctacgtcc aggagcgcac catctctttc aaggacgacg gtacctacaa
     3901 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     3961 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca acttcaacag
     4021 ccacaacgtc tatatcacgg ccgacaagca gaagaacggc atcaaggcta acttcaagat
     4081 ccgccacaac gttgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     4141 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagccatc agtccgccct
     4201 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     4261 cgggtccgga cgaatgaagc agttggagga cgagatcgaa gaattggaat ccgaaaacta
     4321 ccacttggag aataggatcg cacggttgcg aaagagaatt ggggaacgcg gcggctctgg
     4381 gggaagcggt ggatctggag gaagtggcgg gtctggtggt tcagggggtt ccggcgggag
     4441 tggaggaagc ggcggaagcg gaggcagcgg tgggtccaga atgaaacagc ttcgcgaccg
     4501 aatagaggag cttcggagca gaaattatca tctcagaaat gaaattgcca gattggagaa
     4561 ggagatcggg gagagactgt acaaggcggc cgcggagttc ccagagcaga aagcaggaga
     4621 agctattaat cagcccatga tgatggctgc taggcagttg catgacgagg ctcggaaatg
     4681 gtctagcaag ggtaacgaca tcattgctgc tgctaaacga atggcgctgc tcatggctga
     4741 gatgtcacgc ctggtgcgag gaggcagcgg aaacaagcgt gccctcatcc agtgtgcaaa
     4801 agatattgct aaggcatcgg atgaagtcac tcgattggcc aaagaggtgg ccaagcaatg
     4861 tactgataaa cgcattagaa caaacctctt acaggtctgt gagcgaatcc caaccatcag
     4921 cacgcagctc aaaattcttt ccacagtgaa agctaccatg ctgggcagga ctaacatcag
     4981 cgatgaagaa tcagaacagg caactgagat gttggttcat aacgcccaga acctcatgca
     5041 gtctgtgaag gaaactgtga gagaagctga agcagcatcc attaagataa gaacagatgc
     5101 cggattcact ctgcgctggg tcagaaagac cccatggtat cagtaagaat tctgcagata
     5161 tccagcacag tggcggccgc tcgagtctag agggcccgtt taaacccgct gatcagcctc
     5221 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     5281 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     5341 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     5401 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     5461 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     5521 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     5581 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     5641 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     5701 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     5761 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     5821 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     5881 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     5941 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     6001 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     6061 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     6121 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     6181 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     6241 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
     6301 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
     6361 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     6421 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     6481 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
     6541 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     6601 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     6661 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     6721 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     6781 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     6841 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     6901 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     6961 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     7021 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     7081 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     7141 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
     7201 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     7261 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     7321 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     7381 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
     7441 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     7501 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     7561 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     7621 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     7681 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     7741 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     7801 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     7861 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     7921 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     7981 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     8041 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     8101 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     8161 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     8221 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     8281 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     8341 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     8401 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     8461 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     8521 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     8581 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     8641 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     8701 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     8761 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     8821 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     8881 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     8941 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     9001 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     9061 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     9121 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     9181 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     9241 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     9301 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     9361 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     9421 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     9481 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     9541 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     9601 ttccccgaaa agtgccacct gacgtc
//
