LOCUS       sequence-462981-        9545 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Parallel coiled-coil with (GGS)3 linker to prone condensation  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9545)
  AUTHORS   Liu S, Liu J, Foote A, Ogasawara H, Al Abdullatif S, Batista VS, 
            Salaita K
  TITLE     Digital and Tunable Genetically Encoded Tension Sensors Based on 
            Engineered Coiled-Coils.
  JOURNAL   Angew Chem Int Ed Engl. 2025 Jan 13:e202407359. doi: 
            10.1002/anie.202407359.
  PUBMED    39805005
REFERENCE   2  (bases 1 to 9545)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2025 Jan 13:e202407359. doi: 10.1002/anie.202407359."
COMMENT     Sequence Label: pcDNA3.1-Vh-clover-GCN4-(GGS)3-Vt
FEATURES             Location/Qualifiers
     source          1..9545
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3623..3644)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     4231..4252
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5136..5153)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5142..5366
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5412..5840
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5499..5518)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5709..5730
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5849..5869)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5854..6183
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6034..6169
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6096..6115
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6250..7044
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6304..6323)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6914..6933
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7218..7339
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7255..7274)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7309..7328
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7388..7404)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7388..7404)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7401..7423)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7412..7428
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7436..7442,7443..7460,7461..7466))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7481..7502
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7619..7636)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7790..8378)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7870..7889)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8549..9340,9341..9409))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9172..9191
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9410..9514)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttgcca tgcccgtctt ccacacgcgc accatcgaga gcatcttgga
      961 gcccgtggct cagcagatct cccacctggt catcatgcac gaggaggggg aggtagacgg
     1021 caaggccatc ccggacctca ccgcccccgt gtcggccgtg caggccgctg tcagcaacct
     1081 ggtgcgggtt ggaaaagaaa ctgtgcagac aacagaagac cagatcttga aaagggatat
     1141 gccaccagca ttcatcaaag tagagaatgc ctgcaccaag ctcgttcgag cagcccagat
     1201 gctgcaagca gatccttatt cagtaccagc tcgtgactac ctaattgatg gatcaagagg
     1261 catcctttct ggaacatcag acttacttct gacatttgat gaagcggagg tccgtaaaat
     1321 catccgtgtc tgcaaaggaa tattggaata tctgactgtg gcagaagtag tagagactat
     1381 ggaggatttg gtgacatata caaagaatct agggccagga atgacaaaga tggcgaaaat
     1441 gatcgatgag agacaacagg aattaactca tcaggaacat agggttatgc tggtgaactc
     1501 catgaatact gtgaaggagc tattgcctgt acttatttca gctatgaaga tctttgtaac
     1561 cacaaaaaac actaaaagcc agggaataga agaggccttg aaaaatcgca atttcacagt
     1621 agagaaaatg agtgctgaga taaatgaaat aatccgtgta ttacaactca cttcctggga
     1681 tgaagatgcc tgggccagca aggacactga agccatgaaa agagctttag ccctaataga
     1741 ttcaaagatg aaccaggcaa aaggctggct gagagatcca aacgcacctc caggggatgc
     1801 tggtgagcaa gcaataaggc agatccttga tgaagctgga aaagcaggag aattgtgtgc
     1861 aggcaaagaa cgcagggaga ttctgggaac atgcaaaact ttgggccaaa tgactgatca
     1921 acttgctgat ctccgagcta gaggacaggg tgctacaccg atggcaatgc agaaggcaca
     1981 gcaggtgtca caaggcctgg atttgctcac tgcaaaagtg gagaatgcag cccggaaatt
     2041 ggaggccatg acaaactcta agcaggctat tgcaaagaag attgatgctg ctcagaactg
     2101 gcttgcggat cccaacggcg gaagtgaagg agaagaacac attcgaggaa ttatgtctga
     2161 agcaaggaaa gttgcagaat tgtgtgagga gcctaaagaa agagatgata tccttcgctc
     2221 cttgggggaa atctctgctc tgacagctaa gctgtcagat ctgcgacgac atgggaaagg
     2281 cgactctcct gaggcccgtg cattggccaa gcaaatagct acatcacttc agaacttaca
     2341 gtccaaaaca aacagagctg tagcaaatac taggccagtt aaagctgctg tccatttgga
     2401 gggcaagatt gagcaagccc agaggtggat agataatcct acagttgatg atcggggagt
     2461 aggccaggca gcaattcggg gtttggttgc agaaggtcgt cgtctagcca atgtcatgat
     2521 gggaccttat cgtcaggacc tgcttgccaa atgtgaccgt gtagaccagc tggctgctca
     2581 gcttgctgac cttgcagcaa gaggggaagg agagtctcct caggctaggg caattgctgc
     2641 tcagcttcag gactccctga aggatctcaa agcacggatg caagaagcaa tgacccagga
     2701 ggtgtctgat gttttcagtg acactacaac ccctattaag ttgttagcag tagcagccac
     2761 tgctccttct gatactccca atagagaaga ggtgtttgaa gaaagagcag caaattttga
     2821 aaaccatgct gctagactgg gagcaacagc agaaaaagca gctgcagttg gaactgctaa
     2881 taaaactact gtggaaggca ttcaggcaac agtcaaatct gcaagggagc ttacaccaca
     2941 ggtagtatcg gctgctcgaa tcctcctgag aaatcctgga aatcaagctg cttatgagca
     3001 ttttgagaca atgaaaaacc aatggattga taatgtagaa aagatgacag ggctggtgga
     3061 tgaggccatc gataccaagt ctctgttgga tgcatcagaa gaggctatta agaaggatct
     3121 tgataaatgt aaagttgcaa tggccaatat gcaacctcag atgctggtag ctggagccac
     3181 cagcattgct agacgagcga accgcatcct gcttgtggca aaacgggagg ttgaaaattc
     3241 agaagaccct aaattcaggg aggctgttaa agcagcttct gatgagctga gcaaaaccat
     3301 atcaccgatg gtaatggatg ctaaagctgt agcaggaaat atctctgatc ctggtttgca
     3361 gaagagtttc ttggattctg gatacaggat tctgggagct gtggccaaag tcagagaagc
     3421 ctttcagcct caggagccag actttccccc tcctcctcct gacctagagc atctccatct
     3481 gactgatgag cttgctcctc caaaaccacc acttccagaa ggtgaggttc ccccacccag
     3541 accaccaccc cctgaagaaa aagatgaggt cgagatgatg gtgagcaagg gcgaggagct
     3601 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     3661 cagcgtccgc ggcgagggcg agggcgatgc caccaacggc aagctgaccc tgaagttcat
     3721 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg
     3781 cgtggcctgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     3841 catgcccgaa ggctacgtcc aggagcgcac catctctttc aaggacgacg gtacctacaa
     3901 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     3961 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca acttcaacag
     4021 ccacaacgtc tatatcacgg ccgacaagca gaagaacggc atcaaggcta acttcaagat
     4081 ccgccacaac gttgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     4141 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagccatc agtccgccct
     4201 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     4261 cgggtccgga aggatgaaac aactcgagga cgagatagaa gaacttgagt ccgagaacta
     4321 ccacctggaa aaccggatcg cccgccttcg gaagagaatc ggggagcgag gtggttccgg
     4381 ggggagcggc ggctctcgca tgaagcagct tcgggataga atcgaagaat tgaggtcacg
     4441 aaactaccac cttagaaacg aaatcgcaag actggaaaaa gagataggag aacgactgta
     4501 caaggcggcc gcggagttcc cagagcagaa agcaggagaa gctattaatc agcccatgat
     4561 gatggctgct aggcagttgc atgacgaggc tcggaaatgg tctagcaagg gtaacgacat
     4621 cattgctgct gctaaacgaa tggcgctgct catggctgag atgtcacgcc tggtgcgagg
     4681 aggcagcgga aacaagcgtg ccctcatcca gtgtgcaaaa gatattgcta aggcatcgga
     4741 tgaagtcact cgattggcca aagaggtggc caagcaatgt actgataaac gcattagaac
     4801 aaacctctta caggtctgtg agcgaatccc aaccatcagc acgcagctca aaattctttc
     4861 cacagtgaaa gctaccatgc tgggcaggac taacatcagc gatgaagaat cagaacaggc
     4921 aactgagatg ttggttcata acgcccagaa cctcatgcag tctgtgaagg aaactgtgag
     4981 agaagctgaa gcagcatcca ttaagataag aacagatgcc ggattcactc tgcgctgggt
     5041 cagaaagacc ccatggtatc agtaagaatt ctgcagatat ccagcacagt ggcggccgct
     5101 cgagtctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     5161 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     5221 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     5281 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     5341 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     5401 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     5461 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     5521 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     5581 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     5641 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     5701 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     5761 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     5821 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     5881 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     5941 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     6001 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     6061 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     6121 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     6181 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga
     6241 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     6301 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     6361 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     6421 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     6481 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     6541 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     6601 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     6661 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     6721 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     6781 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     6841 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     6901 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     6961 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     7021 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga
     7081 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     7141 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     7201 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     7261 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     7321 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
     7381 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     7441 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     7501 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     7561 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     7621 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     7681 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     7741 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     7801 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     7861 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     7921 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     7981 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     8041 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     8101 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     8161 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     8221 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     8281 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     8341 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     8401 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     8461 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     8521 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     8581 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     8641 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     8701 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     8761 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     8821 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     8881 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     8941 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     9001 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     9061 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     9121 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     9181 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     9241 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     9301 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     9361 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     9421 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     9481 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     9541 acgtc
//
