LOCUS       sequence-462997-        8863 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression of Myt1l.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8863)
  AUTHORS   Wang NB, Lende-Dorn BA, Beitz AM, Han P, Adewumi HO, O'Shea TM, 
            Galloway KE
  TITLE     Proliferation history and transcription factor levels drive direct 
            conversion to motor neurons.
  JOURNAL   Cell Syst. 2025 Apr 16;16(4):101205. doi: 
            10.1016/j.cels.2025.101205. Epub 2025 Mar 13.
  PUBMED    40086434
REFERENCE   2  (bases 1 to 8863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Syst."; date: "2025-04-16"; volume: "16(4):101205. doi"; pages: " 
            10.1016/j.cels.2025.101205. Epub 2025 Mar 13"
COMMENT     Sequence Label: pMXs-hMyt1l
FEATURES             Location/Qualifiers
     source          1..8863
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(113..217)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             247..840
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    903..1260
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1319..1735
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1665..1687
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1703..1719
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1745..2119
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     2057..2073
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     protein_bind    2162..2186
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5784..5808)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5825..6413
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5878..5898)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6296..6307)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6593..7062
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(7192..7209)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7363..7951)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7443..7462)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8115..8863,1..43,44..112))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8738..8757
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
       61 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
      121 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
      181 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
      241 ctgcataatg aaagacccca cctgtaggtt tggcaagcta gcttaagtaa cgccattttg
      301 caaggcatgg aaaaatacat aactgagaat agaaaagttc agatcaaggt caggaacaga
      361 tggaacagct gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc
      421 agggccaaga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
      481 ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtcca gccctcagca
      541 gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc
      601 cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg
      661 agctcaataa aagagcccac aacccctcac tcggcgcgcc agtcctccga ttgactgagt
      721 cgcccgggta cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg
      781 ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcatt
      841 tgggggctcg tccgggatcg ggagacccct gcccagggac caccgaccca ccaccgggag
      901 gtaagctggc cagcaactta tctgtgtctg tccgattgtc tagtgtctat gactgatttt
      961 atgcgcctgc gtcggtacta gttagctaac tagctctgta tctggcggac ccgtggtgga
     1021 actgacgagt tcggaacacc cggccgcaac cctgggagac gtcccaggga cttcgggggc
     1081 cgtttttgtg gcccgacctg agtccaaaaa tcccgatcgt tttggactct ttggtgcacc
     1141 cccctaatag gagggatatg tggttctggt aggagacgag aacctaaaac agttcccgcc
     1201 tccgtctgaa tttttgcttt cggtttggga ccgaagccgc gccgcgcgtc ttgtctgctg
     1261 cagcatcgtt ctgtgttgtc tctgtctgac tgtgtttctg tatttgtctg aaaattaggg
     1321 ccagactgtt accactccct taagtttgac cttaggtcac tggaaagatg tcgagcggat
     1381 cgctcacaac cagtcggtag atgtcaagaa gagacgttgg gttaccttct gctctgcaga
     1441 atggccaacc tttaacgtcg gatggccgcg agacggcacc tttaaccgag acctcatcac
     1501 ccaggttaag atcaaggtct tttcacctgg cccgcatgga cacccagacc aggtccccta
     1561 catcgtgacc tgggaagcct tggcttttga cccccctccc tgggtcaagc cctttgtaca
     1621 ccctaagcct ccgcctcctc ttcctccatc cgccccgtct ctcccccttg aacctcctcg
     1681 ttcgaccccg cctcgatcct ccctttatcc agccctcact ccttctctag gcgcccccat
     1741 atggccatat gagatcttat atggggcacc cccgcccctt gtaaacttcc ctgaccctga
     1801 catgacaaga gttactaaca gcccctctct ccaagctcac ttacaggctc tctacttagt
     1861 ccagcacgaa gtctggagac ctctggcggc agcctaccaa gaacaactgg accgaccggt
     1921 ggtacctcac ccttaccgag tcggcgacac agtgtgggtc cgccgacacc agactaagaa
     1981 cctagaacct cgctggaaag gaccttacac agtcctgctg accaccccca ccgccctcaa
     2041 agtagacggc atcgcagctt ggatacacgc cgcccacgtg aaggctgccg accccggggg
     2101 tggaccatcc tctagactgc cggatctagc tagttaatcg acggtatcga taagctatca
     2161 aacaagtttg tacaaaaaag caggctccgc ggccgccccc ttcaccatgg aggtggacac
     2221 cgaggagaag cggcatcgca cgcggtccaa aggggttcga gttcccgtgg aaccagccat
     2281 acaagagctg ttcagctgtc ccacccctgg ctgtgacggc agtggtcatg tcagtggcaa
     2341 atatgcaaga cacagaagtg tatatggttg tcccttggcg aaaaaaagaa aaacacaaga
     2401 taaacagccc caggaacctg ctcctaaacg aaagccattt gccgtgaaag cagacagctc
     2461 ctcagtggat gagtgtgacg acagtgatgg gactgaggac atggatgaga aggaggagga
     2521 tgagggggag gagtactccg aggacaatga cgagccaggg gatgaggacg aggaggacga
     2581 ggagggggac cgggaggagg aggaggagat cgaggaggag gatgaggacg atgacgagga
     2641 tggagaagat gtggaggatg aagaagagga agaggaggag gaggaggagg aggaagagga
     2701 agaagaaaac gaagaccatc aaatgaattg tcacaatact cgaataatgc aagacacaga
     2761 aaaggatgat aacaataatg acgaatatga caattacgat gaactggtgg ccaagtcatt
     2821 gttaaacctc ggcaaaatcg ctgaggatgc agcctaccgg gccaggactg agtcagaaat
     2881 gaacagcaat acctccaata gtctggaaga cgatagtgac aaaaacgaaa acctgggtcg
     2941 gaaaagtgag ttgagtttag acttagacag tgatgttgtt agagaaacag tggactccct
     3001 taaactatta gcccaaggac acggtgttgt gctctcagaa aacatgaatg acagaaatta
     3061 tgcagacagc atgtcgcagc aagacagtag aaatatgaat tacgtcatgt tggggaagcc
     3121 catgaacaat ggactcatgg aaaagatggt ggaggagagc gatgaggagg tgtgtctgag
     3181 cagtctggag tgtttgagga atcagtgctt cgacctggcc aggaagctca gtgagaccaa
     3241 cccgcaggag aggaatccgc agcagaacat gaacatccgt cagcatgtcc ggccagaaga
     3301 ggacttccca ggaaggacgc cggacagaaa ctactcggac atgctgaacc tcatgcggct
     3361 ggaggagcag ttgagccccc ggtcgagagt gtttgccagc tgtgcgaagg aggatgggtg
     3421 tcatgagcgg gacgacgata ccacctctgt gaactcggac aggtctgaag aggtgttcga
     3481 catgaccaag gggaacctga ccctgctgga gaaagccatc gctttggaaa cggaaagagc
     3541 aaaggccatg agggagaaga tggccatgga agctgggagg agggacaata tgaggtcata
     3601 tgaggaccag tctccgagac aacttcccgg ggaggacaga aagcctaaat ccagtgacag
     3661 ccatgtcaaa aagccatact atgatccctc aagaacagaa aagaaagaga gcaagtgtcc
     3721 aacccccggg tgtgatggaa ccggccacgt aactgggctg tacccacatc accgcagcct
     3781 gtccggatgc ccgcacaaag atagggtccc tccagaaatc cttgccatgc atgaaagtgt
     3841 cctcaagtgc cccactccgg gctgcacggg gcgcgggcat gtcaacagca acaggaactc
     3901 ccaccgaagc ctctccggat gcccgatcgc tgcagcagag aaactggcca aggcacagga
     3961 aaagcaccag agctgcgacg tgtccaagtc cagccaggcc tcggaccgcg tgctcaggcc
     4021 aatgtgcttt gtgaagcagc tggagattcc tcagtatggc tacagaaaca atgtccccac
     4081 aactacgccg cgttccaacc tggccaagga gctcgagaaa tattccaaga cctcgtttga
     4141 atacaacagt tacgacaacc atacttatgg caagcgagcc atagctccca aggtgcaaac
     4201 cagggatata tcccccaaag gatatgatga tgcgaagcgg tactgcaagg accccagccc
     4261 cagcagcagc agcaccagca gctacgcgcc cagcagcagc agcaacctga gctgcggcgg
     4321 gggcagcagc gccagcagca cgtgcagcaa gagcagcttc gactacacgc acgacatgga
     4381 ggcggcccac atggcggcca ccgccatcct caacctgtcc acgcgctgcc gcgagatgcc
     4441 gcagaacctg agcaccaagc cgcaggacct gtgcgccacg cggaaccctg acatggaggt
     4501 ggatgagaac gggaccctgg acctcagcat gaacaagcag aggccgcggg acagctgctg
     4561 ccccatcctg acccctctgg agcccatgtc cccccagcag caggcagtga tgaacaaccg
     4621 gtgtttccag ctgggcgagg gcgactgctg ggacttgccc gtagactaca ccaaaatgaa
     4681 accccggagg atagacgagg acgagtccaa agacattact ccagaagact tggacccatt
     4741 ccaggaggct ctagaagaaa gacggtatcc cggggaggtg accatcccaa gtcccaaacc
     4801 caagtaccct cagtgcaagg agagcaaaaa ggacttaata actctgtctg gctgccccct
     4861 ggcggacaaa agcattcgaa gtatgctggc caccagctcc caagaactca agtgccccac
     4921 gcctggctgt gatggttctg gacatatcac cggcaattat gcttctcatc ggagcctttc
     4981 aggttgccca agagcaaaga aaagtggtat caggatagca cagagcaaag aagataaaga
     5041 agatcaagaa cccatcaggt gtccggtccc cgggtgcgac ggccagggcc acatcactgg
     5101 gaagtacgcg tcccatcgca gcgcctccgg gtgccccttg gcggccaaga ggcagaaaga
     5161 cgggtacctg aatggctccc agttctcctg gaagtcggtc aagacggaag gcatgtcctg
     5221 ccccacgcca ggatgcgacg gctcaggcca cgtcagcggc agcttcctca cacaccgcag
     5281 cttgtcagga tgcccgagag ccacgtcagc gatgaagaag gcaaagcttt ctggagagca
     5341 gatgctgacc atcaaacagc gggccagcaa cggtatagaa aatgatgaag aaatcaaaca
     5401 gttagatgaa gaaatcaagg agctaaatga atccaattcc cagatggaag ccgatatgat
     5461 taaactcaga actcagatta ccacgatgga gagcaacctg aagaccatcg aagaggagaa
     5521 caaagtgatt gagcagcaga acgagtctct cctccacgag ctggcgaacc tgagccagtc
     5581 tctgatccac agcctggcta acatccagct gccgcacatg gatccaatca atgaacaaaa
     5641 ttttgatgct tacgtgacta ctttgacgga aatgtataca aatcaagatc gttatcagag
     5701 tccagaaaat aaagccctac tggaaaatat aaagcaggct gtgagaggaa ttcaggtctg
     5761 aggcggcaag ggtgggcgcg ccgacccagc tttcttgtac aaagtggttc gatagcttat
     5821 cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     5881 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     5941 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     6001 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     6061 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     6121 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     6181 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     6241 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     6301 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     6361 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata
     6421 ccgtcgacga taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac
     6481 cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc atggaaaaat
     6541 acataactga gaatagagaa gttcagatca aggtcaggaa cagatggaac agctgaatat
     6601 gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat
     6661 ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca
     6721 gggccaagaa cagatggtcc ccagatgcgg tccagccctc agcagtttct agagaaccat
     6781 cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc
     6841 aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc
     6901 ccacaacccc tcactcgggg cgccagtcct ccgattgact gagtcgcccg ggtacccgtg
     6961 tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg
     7021 tctcctctga gtgattgact acccgtcagc gggggtcttt cacatgcagc atgtatcaaa
     7081 attaatttgg ttttttttct taagtattta cattaaatgg ccatagttgc attaatgaat
     7141 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     7201 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     7261 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     7321 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     7381 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     7441 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     7501 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     7561 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     7621 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     7681 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     7741 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     7801 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     7861 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     7921 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     7981 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     8041 gatcttcacc tagatccttt tgcggccggc cgcaaatcaa tctaaagtat atatgagtaa
     8101 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     8161 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     8221 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     8281 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     8341 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     8401 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     8461 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     8521 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     8581 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     8641 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     8701 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     8761 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     8821 ccgctgttga gatccagttc gatgtaaccc actcgtgcac cca
//
