LOCUS       sequence-463011-        6520 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression of Lhx3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6520)
  AUTHORS   Wang NB, Lende-Dorn BA, Beitz AM, Han P, Adewumi HO, O'Shea TM, 
            Galloway KE
  TITLE     Proliferation history and transcription factor levels drive direct 
            conversion to motor neurons.
  JOURNAL   Cell Syst. 2025 Apr 16;16(4):101205. doi: 
            10.1016/j.cels.2025.101205. Epub 2025 Mar 13.
  PUBMED    40086434
REFERENCE   2  (bases 1 to 6520)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Syst."; date: "2025-04-16"; volume: "16(4):101205. doi"; pages: " 
            10.1016/j.cels.2025.101205. Epub 2025 Mar 13"
COMMENT     Sequence Label: pMXs-mLhx3
FEATURES             Location/Qualifiers
     source          1..6520
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     117..133
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     protein_bind    222..246
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1501..1525)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    1542..2130
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1595..1615)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2013..2024)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2310..2779
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(2909..2926)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3080..3668)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3160..3179)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3832..4623,4624..4692))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4455..4474
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4693..4797)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             4827..5420
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    5483..5840
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             5899..6315
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     6245..6267
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     6283..6299
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    join(6325..6520,1..179)
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
ORIGIN
        1 tcggcgacac agtgtgggtc cgccgacacc agactaagaa cctagaacct cgctggaaag
       61 gaccttacac agtcctgctg accaccccca ccgccctcaa agtagacggc atcgcagctt
      121 ggatacacgc cgcccacgtg aaggctgccg accccggggg tggaccatcc tctagactgc
      181 cggatctagc tagttaatcg acggtatcga taagctatca aacaagtttg tacaaaaaag
      241 caggctccac caggaaccaa ttcagtcgac tggatccggt acgccaccat gctgctagaa
      301 gcagaactcg attgccaccg agagaggccc ggtgcccctg gagcttctgc cctctgtacc
      361 ttcagcagga ctccagagat cccgatgtgt gcaggctgtg accagcacat cttggaccgt
      421 ttcatcctta aggctctgga ccgacattgg cacagcaagt gtctcaagtg cagtgactgc
      481 cacgtccctc tggctgagcg ctgcttcagc cgcggggaga gcgtctactg caaagacgac
      541 ttctttaagc gcttcgggac caagtgcgcc gcatgccagc tgggcatccc gcccacgcag
      601 gtggtgcgcc gcgcccagga cttcgtgtac cacctgcatt gcttcgcctg cgtggtctgc
      661 aagcggcagc tggccacggg cgacgagttc tacctcatgg aagacagccg gctggtgtgc
      721 aaggcggact acgaaacagc caagcagcga gaagccgagg ccacagccaa gcggccgcgc
      781 accaccatca ccgccaagca gctggagacg ctgaagagcg cctacaacac ttcgcccaag
      841 ccggcgcgcc acgtgcgcga gcagctctcc tccgagaccg gcctggacat gcgagtggtg
      901 caggtgtggt tccagaatcg ccgggctaag gaaaagagac tgaagaaaga cgctggccgg
      961 cagcgctggg gacagtattt ccgcaatatg aagcgctccc gcggcagttc caagtccgac
     1021 aaggacagca tccaggaggg acaagacagc gacgccgaag tctccttcac tgatgagccg
     1081 tccatggctg acatggggcc tgctaatggc ctgtacagca gcctgggaga gcctgcccct
     1141 gcgttgggcc ggcccgtagg aggcctgggc agctttaccc tggatcacgg aggcttgacg
     1201 ggtccagagc agtaccgaga gctacgccca ggcagcccct atggcatccc cccatctcct
     1261 gcagcccccc agagccttcc tggcccccag cctctcctct ccagcctggt atacccagac
     1321 accaacttga gccttgtccc ttcagggccc ccaggtggac ccccacccat gagggtgctg
     1381 gctggaaatg ggcccagctc cgacctgtcc acagagagca gttctggcta cccagacttt
     1441 cctgctagcc ctgcttcctg gctggatgaa gtagaccatg ctcagttcta agatatctag
     1501 acccagcttt cttgtacaaa gtggttcgat agcttatcga taatcaacct ctggattaca
     1561 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     1621 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     1681 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     1741 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     1801 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     1861 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     1921 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     1981 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     2041 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     2101 gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacgataa aataaaagat
     2161 tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt ttggcaagct
     2221 agcttaagta acgccatttt gcaaggcatg gaaaaataca taactgagaa tagagaagtt
     2281 cagatcaagg tcaggaacag atggaacagc tgaatatggg ccaaacagga tatctgtggt
     2341 aagcagttcc tgccccggct cagggccaag aacagatgga acagctgaat atgggccaaa
     2401 caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggtcccca
     2461 gatgcggtcc agccctcagc agtttctaga gaaccatcag atgtttccag ggtgccccaa
     2521 ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt ctcgcttctg
     2581 ttcgcgcgct tctgctcccc gagctcaata aaagagccca caacccctca ctcggggcgc
     2641 cagtcctccg attgactgag tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt
     2701 tgcatccgac ttgtggtctc gctgttcctt gggagggtct cctctgagtg attgactacc
     2761 cgtcagcggg ggtctttcac atgcagcatg tatcaaaatt aatttggttt tttttcttaa
     2821 gtatttacat taaatggcca tagttgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     2881 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     2941 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     3001 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     3061 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     3121 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     3181 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     3241 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     3301 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     3361 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     3421 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     3481 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     3541 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     3601 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     3661 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     3721 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttgc
     3781 ggccggccgc aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     3841 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     3901 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     3961 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     4021 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     4081 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     4141 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     4201 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     4261 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     4321 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     4381 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     4441 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     4501 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     4561 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     4621 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     4681 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     4741 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     4801 atttccccga aaagtgccac ctgcataatg aaagacccca cctgtaggtt tggcaagcta
     4861 gcttaagtaa cgccattttg caaggcatgg aaaaatacat aactgagaat agaaaagttc
     4921 agatcaaggt caggaacaga tggaacagct gaatatgggc caaacaggat atctgtggta
     4981 agcagttcct gccccggctc agggccaaga acagatggaa cagctgaata tgggccaaac
     5041 aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag
     5101 atgcggtcca gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag
     5161 gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt
     5221 tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac tcggcgcgcc
     5281 agtcctccga ttgactgagt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt
     5341 gcatccgact tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc
     5401 gtcagcgggg gtctttcatt tgggggctcg tccgggatcg ggagacccct gcccagggac
     5461 caccgaccca ccaccgggag gtaagctggc cagcaactta tctgtgtctg tccgattgtc
     5521 tagtgtctat gactgatttt atgcgcctgc gtcggtacta gttagctaac tagctctgta
     5581 tctggcggac ccgtggtgga actgacgagt tcggaacacc cggccgcaac cctgggagac
     5641 gtcccaggga cttcgggggc cgtttttgtg gcccgacctg agtccaaaaa tcccgatcgt
     5701 tttggactct ttggtgcacc cccctaatag gagggatatg tggttctggt aggagacgag
     5761 aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggga ccgaagccgc
     5821 gccgcgcgtc ttgtctgctg cagcatcgtt ctgtgttgtc tctgtctgac tgtgtttctg
     5881 tatttgtctg aaaattaggg ccagactgtt accactccct taagtttgac cttaggtcac
     5941 tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa gagacgttgg
     6001 gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg agacggcacc
     6061 tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg cccgcatgga
     6121 cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga cccccctccc
     6181 tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc cgccccgtct
     6241 ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc agccctcact
     6301 ccttctctag gcgcccccat atggccatat gagatcttat atggggcacc cccgcccctt
     6361 gtaaacttcc ctgaccctga catgacaaga gttactaaca gcccctctct ccaagctcac
     6421 ttacaggctc tctacttagt ccagcacgaa gtctggagac ctctggcggc agcctaccaa
     6481 gaacaactgg accgaccggt ggtacctcac ccttaccgag
//
