LOCUS       sequence-463021-        6187 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression of c-Myc.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6187)
  AUTHORS   Wang NB, Lende-Dorn BA, Beitz AM, Han P, Adewumi HO, O'Shea TM, 
            Galloway KE
  TITLE     Proliferation history and transcription factor levels drive direct 
            conversion to motor neurons.
  JOURNAL   Cell Syst. 2025 Apr 16;16(4):101205. doi: 
            10.1016/j.cels.2025.101205. Epub 2025 Mar 13.
  PUBMED    40086434
REFERENCE   2  (bases 1 to 6187)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Syst."; date: "2025-04-16"; volume: "16(4):101205. doi"; pages: " 
            10.1016/j.cels.2025.101205. Epub 2025 Mar 13"
COMMENT     Sequence Label: pMXs-cMyc
FEATURES             Location/Qualifiers
     source          1..6187
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(100..204)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             234..827
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    890..1247
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1306..1722
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1652..1674
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1690..1706
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1732..2106
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     2044..2060
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     protein_bind    2157..2181
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3563..3587)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     LTR             3904..4373
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4503..4520)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4674..5262)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4754..4773)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5426..6187,1..30,31..99))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6049..6068
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
       61 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
      121 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
      181 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg cataatgaaa
      241 gaccccacct gtaggtttgg caagctagct taagtaacgc cattttgcaa ggcatggaaa
      301 aatacataac tgagaataga aaagttcaga tcaaggtcag gaacagatgg aacagctgaa
      361 tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca
      421 gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc
      481 tcagggccaa gaacagatgg tccccagatg cggtccagcc ctcagcagtt tctagagaac
      541 catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta
      601 accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag
      661 agcccacaac ccctcactcg gcgcgccagt cctccgattg actgagtcgc ccgggtaccc
      721 gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga
      781 gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcatttgg gggctcgtcc
      841 gggatcggga gacccctgcc cagggaccac cgacccacca ccgggaggta agctggccag
      901 caacttatct gtgtctgtcc gattgtctag tgtctatgac tgattttatg cgcctgcgtc
      961 ggtactagtt agctaactag ctctgtatct ggcggacccg tggtggaact gacgagttcg
     1021 gaacacccgg ccgcaaccct gggagacgtc ccagggactt cgggggccgt ttttgtggcc
     1081 cgacctgagt ccaaaaatcc cgatcgtttt ggactctttg gtgcaccccc ctaataggag
     1141 ggatatgtgg ttctggtagg agacgagaac ctaaaacagt tcccgcctcc gtctgaattt
     1201 ttgctttcgg tttgggaccg aagccgcgcc gcgcgtcttg tctgctgcag catcgttctg
     1261 tgttgtctct gtctgactgt gtttctgtat ttgtctgaaa attagggcca gactgttacc
     1321 actcccttaa gtttgacctt aggtcactgg aaagatgtcg agcggatcgc tcacaaccag
     1381 tcggtagatg tcaagaagag acgttgggtt accttctgct ctgcagaatg gccaaccttt
     1441 aacgtcggat ggccgcgaga cggcaccttt aaccgagacc tcatcaccca ggttaagatc
     1501 aaggtctttt cacctggccc gcatggacac ccagaccagg tcccctacat cgtgacctgg
     1561 gaagccttgg cttttgaccc ccctccctgg gtcaagccct ttgtacaccc taagcctccg
     1621 cctcctcttc ctccatccgc cccgtctctc ccccttgaac ctcctcgttc gaccccgcct
     1681 cgatcctccc tttatccagc cctcactcct tctctaggcg cccccatatg gccatatgag
     1741 atcttatatg gggcaccccc gccccttgta aacttccctg accctgacat gacaagagtt
     1801 actaacagcc cctctctcca agctcactta caggctctct acttagtcca gcacgaagtc
     1861 tggagacctc tggcggcagc ctaccaagaa caactggacc gaccggtggt acctcaccct
     1921 taccgagtcg gcgacacagt gtgggtccgc cgacaccaga ctaagaacct agaacctcgc
     1981 tggaaaggac cttacacagt cctgctgacc acccccaccg ccctcaaagt agacggcatc
     2041 gcagcttgga tacacgccgc ccacgtgaag gctgccgacc ccgggggtgg accatcctct
     2101 agactgccgg atctagctag ttaattaagg atcccagtgt ggtggtacgg gaaatcacaa
     2161 gtttgtacaa aaaagcaggc tccaccatgg caccatgccc ctcaacgtga acttcaccaa
     2221 caggaactat gacctcgact acgactccgt acagccctat ttcatctgcg acgaggaaga
     2281 gaatttctat caccagcaac agcagagcga gctgcagccg cccgcgccca gtgaggatat
     2341 ctggaagaaa ttcgagctgc ttcccacccc gcccctgtcc ccgagccgcc gctccgggct
     2401 ctgctctcca tcctatgttg cggtcgctac gtccttctcc ccaagggaag acgatgacgg
     2461 cggcggtggc aacttctcca ccgccgatca gctggagatg atgaccgagt tacttggagg
     2521 agacatggtg aaccagagct tcatctgcga tcctgacgac gagaccttca tcaagaacat
     2581 catcatccag gactgtatgt ggagcggttt ctcagccgct gccaagctgg tctcggagaa
     2641 gctggcctcc taccaggctg cgcgcaaaga cagcaccagc ctgagccccg cccgcgggca
     2701 cagcgtctgc tccacctcca gcctgtacct gcaggacctc accgccgccg cgtccgagtg
     2761 cattgacccc tcagtggtct ttccctaccc gctcaacgac agcagctcgc ccaaatcctg
     2821 tacctcgtcc gattccacgg ccttctctcc ttcctcggac tcgctgctgt cctccgagtc
     2881 ctccccacgg gccagccctg agcccctagt gctgcatgag gagacaccgc ccaccaccag
     2941 cagcgactct gaagaagagc aagaagatga ggaagaaatt gatgtggtgt ctgtggagaa
     3001 gaggcaaacc cctgccaaga ggtcggagtc gggctcatct ccatcccgag gccacagcaa
     3061 acctccgcac agcccactgg tcctcaagag gtgccacgtc tccactcacc agcacaacta
     3121 cgccgcaccc ccctccacaa ggaaggacta tccagctgcc aagagggcca agttggacag
     3181 tggcagggtc ctgaagcaga tcagcaacaa ccgcaagtgc tccagcccca ggtcctcaga
     3241 cacggaggaa aacgacaaga ggcggacaca caacgtcttg gaacgtcaga ggaggaacga
     3301 gctgaagcgc agcttttttg ccctgcgtga ccagatccct gaattggaaa acaacgaaaa
     3361 ggcccccaag gtagtgatcc tcaaaaaagc caccgcctac atcctgtcca ttcaagcaga
     3421 cgagcacaag ctcacctctg aaaaggactt attgaggaaa cgacgagaac agttgaaaca
     3481 caaactcgaa cagcttcgaa actctggtgc ataagtcgac tggatccggt accgaattcg
     3541 cggccgcact cgagatatct agacccagct ttcttgtaca aagtggtgat attccagctg
     3601 agcgccggtc gctaccatta ccagttggtc tggtgtcaaa aataataata accgggcagg
     3661 ccatgtctgc ccgtatttcg cgtaaggaaa tccattatgt actatttaaa ctcgagcggc
     3721 cgccagcaca gtggtcgacg ataaaataaa agattttatt tagtctccag aaaaaggggg
     3781 gaatgaaaga ccccacctgt aggtttggca agctagctta agtaacgcca ttttgcaagg
     3841 catggaaaaa tacataactg agaatagaga agttcagatc aaggtcagga acagatggaa
     3901 cagctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc
     3961 caagaacaga tggaacagct gaatatgggc caaacaggat atctgtggta agcagttcct
     4021 gccccggctc agggccaaga acagatggtc cccagatgcg gtccagccct cagcagtttc
     4081 tagagaacca tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat
     4141 ttgaactaac caatcagttc gcttctcgct tctgttcgcg cgcttctgct ccccgagctc
     4201 aataaaagag cccacaaccc ctcactcggg gcgccagtcc tccgattgac tgagtcgccc
     4261 gggtacccgt gtatccaata aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt
     4321 ccttgggagg gtctcctctg agtgattgac tacccgtcag cgggggtctt tcacatgcag
     4381 catgtatcaa aattaatttg gttttttttc ttaagtattt acattaaatg gccatagttg
     4441 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     4501 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     4561 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     4621 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     4681 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     4741 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     4801 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     4861 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     4921 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     4981 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     5041 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     5101 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     5161 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     5221 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     5281 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     5341 ttatcaaaaa ggatcttcac ctagatcctt ttgcggccgg ccgcaaatca atctaaagta
     5401 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     5461 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     5521 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     5581 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     5641 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     5701 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     5761 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     5821 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     5881 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     5941 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     6001 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     6061 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     6121 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     6181 cttcagc
//
