LOCUS       sequence-463030-        6343 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression of NeuroD1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6343)
  AUTHORS   Wang NB, Lende-Dorn BA, Beitz AM, Han P, Adewumi HO, O'Shea TM, 
            Galloway KE
  TITLE     Proliferation history and transcription factor levels drive direct 
            conversion to motor neurons.
  JOURNAL   Cell Syst. 2025 Apr 16;16(4):101205. doi: 
            10.1016/j.cels.2025.101205. Epub 2025 Mar 13.
  PUBMED    40086434
REFERENCE   2  (bases 1 to 6343)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Syst."; date: "2025-04-16"; volume: "16(4):101205. doi"; pages: " 
            10.1016/j.cels.2025.101205. Epub 2025 Mar 13"
COMMENT     Sequence Label: pMXs-hNeuroD1
FEATURES             Location/Qualifiers
     source          1..6343
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(101..120)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      907..1495
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1396..1415
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1649..1666
                     /label=L4440
                     /note="L4440 vector, forward primer"
     LTR             1796..2265
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    2445..3033
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             2551..2562
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     2960..2980
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    3050..3074
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4152..4176)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4219..4593
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     complement(4265..4281)
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             complement(4603..5019)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(4619..4635)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(4651..4673)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    5078..5435
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             5498..6091
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     promoter        6121..6225
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6226..6294,6295..6343,1..743)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
       61 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
      121 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
      181 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
      241 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
      301 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
      361 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
      421 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
      481 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
      541 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
      601 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
      661 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
      721 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
      781 atttgcggcc ggccgcaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
      841 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
      901 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
      961 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     1021 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     1081 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     1141 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     1201 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     1261 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     1321 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     1381 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     1441 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     1501 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     1561 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     1621 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     1681 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca actatggcca
     1741 tttaatgtaa atacttaaga aaaaaaacca aattaatttt gatacatgct gcatgtgaaa
     1801 gacccccgct gacgggtagt caatcactca gaggagaccc tcccaaggaa cagcgagacc
     1861 acaagtcgga tgcaactgca agagggttta ttggatacac gggtacccgg gcgactcagt
     1921 caatcggagg actggcgccc cgagtgaggg gttgtgggct cttttattga gctcggggag
     1981 cagaagcgcg cgaacagaag cgagaagcga actgattggt tagttcaaat aaggcacagg
     2041 gtcatttcag gtccttgggg caccctggaa acatctgatg gttctctaga aactgctgag
     2101 ggctggaccg catctgggga ccatctgttc ttggccctga gccggggcag gaactgctta
     2161 ccacagatat cctgtttggc ccatattcag ctgttccatc tgttcttggc cctgagccgg
     2221 ggcaggaact gcttaccaca gatatcctgt ttggcccata ttcagctgtt ccatctgttc
     2281 ctgaccttga tctgaacttc tctattctca gttatgtatt tttccatgcc ttgcaaaatg
     2341 gcgttactta agctagcttg ccaaacctac aggtggggtc tttcattccc ccctttttct
     2401 ggagactaaa taaaatcttt tattttatcg tcgacggtat cgatgcgggg aggcggccca
     2461 aagggagatc cgactcgtct gagggcgaag gcgaagacgc ggaagaggcc gcagagccgg
     2521 cagcaggccg cgggaaggaa ggtccgctgg attgagggcc gaagggacgt agcagaagga
     2581 cgtcccgcgc agaatccagg tggcaacaca ggcgagcagc caaggaaagg acgatgattt
     2641 ccccgacaac accacggaat tgtcagtgcc caacagccga gcccctgtcc agcagcgggc
     2701 aaggcaggcg gcgatgagtt ccgccgtggc aatagggagg gggaaagcga aagtcccgga
     2761 aaggagctga caggtggtgg caatgcccca accagtgggg gttgcgtcag caaacacagt
     2821 gcacaccacg ccacgttgcc tgacaacggg ccacaactcc tcataaagag acagcaacca
     2881 ggatttatac aaggaggaga aaatgaaagc catacgggaa gcaatagcat gatacaaagg
     2941 cattaaagca gcgtatccac atagcgtaaa aggagcaaca tagttaagaa taccagtcaa
     3001 tctttcacaa attttgtaat ccagaggttg attatcgata agctatcgaa ccactttgta
     3061 caagaaagct gggtctaatc atgaaatatg gcattgagct gggcactcat gactcgctca
     3121 tgatgtgaat ggctatcgaa ggacataata ttgtctatgg ggatctcgca gcgaggggca
     3181 gcggtgcctg agaagattga tccgtggctt tgggcccctg ccagtgtcgc tgcaggatag
     3241 tgcatggtaa aggcataatt tttctcaaac tcggcggacg gttcgtgttt gaaagagaag
     3301 ttgccattga tgctgagcgg cgggctgagg ggtccatcaa aggaagggct ggtgcaatca
     3361 gtcagagggc tttcaaagaa gggctccagc gctgcgctgt aggcgtgcgg cggaggctta
     3421 acgtggaaga catgggagct gtccatggta ccgtaaggcg gactgggcag cccaggcgac
     3481 tggtaggagt aggggtgtac agggaaggaa gcgctggccg tcggcaggtg ggggggcatg
     3541 tcctggttct gctcaggcag aaaagtccga ggattgagtt gcaggcagcc cgcaaccagg
     3601 ttggtggtgg gttgggataa gcccttgcaa agcgtctgaa cgaaggagac caggtctggg
     3661 cttttgcctg agcgcaggat ctccgacaga gcccagatgt agttcttggc caagcgcaga
     3721 gtctcgattt tggacagctt ctgcgtctta gaatagcaag gcaccacctt gcgcaggttg
     3781 tctagcgccg cgttcagtcc gtgcatgcgg ttccgctccc gggcgttagc cttcatgcgt
     3841 ctcaatttaa aacgctccag gcgagcctta gtcatcttct tctttttggg gccgcgtctc
     3901 ttgggctttt gatcgtcatc ctcctcttcc tcttcttcct cctcttccag gtcctcatct
     3961 tcgtcctcct cctctccccc gttcctcagt gagtcctcct ctgcgttcat ggcttcgagg
     4021 tcgtcctcct tcttgtctgc ctcgtgctcc tcgtcctgag aactgagaca ctcgtctgtc
     4081 cagcttggag gaccttgggg ctgaggctcg cccatcagcc cactctcgct gtacgatttg
     4141 gtcatggtgg cagcctgctt ttttgtacaa acttgtttga tagcttatcg ataccgtcga
     4201 ttaactagct agatccggca gtctagagga tggtccaccc ccggggtcgg cagccttcac
     4261 gtgggcggcg tgtatccaag ctgcgatgcc gtctactttg agggcggtgg gggtggtcag
     4321 caggactgtg taaggtcctt tccagcgagg ttctaggttc ttagtctggt gtcggcggac
     4381 ccacactgtg tcgccgactc ggtaagggtg aggtaccacc ggtcggtcca gttgttcttg
     4441 gtaggctgcc gccagaggtc tccagacttc gtgctggact aagtagagag cctgtaagtg
     4501 agcttggaga gaggggctgt tagtaactct tgtcatgtca gggtcaggga agtttacaag
     4561 gggcgggggt gccccatata agatctcata tggccatatg ggggcgccta gagaaggagt
     4621 gagggctgga taaagggagg atcgaggcgg ggtcgaacga ggaggttcaa gggggagaga
     4681 cggggcggat ggaggaagag gaggcggagg cttagggtgt acaaagggct tgacccaggg
     4741 aggggggtca aaagccaagg cttcccaggt cacgatgtag gggacctggt ctgggtgtcc
     4801 atgcgggcca ggtgaaaaga ccttgatctt aacctgggtg atgaggtctc ggttaaaggt
     4861 gccgtctcgc ggccatccga cgttaaaggt tggccattct gcagagcaga aggtaaccca
     4921 acgtctcttc ttgacatcta ccgactggtt gtgagcgatc cgctcgacat ctttccagtg
     4981 acctaaggtc aaacttaagg gagtggtaac agtctggccc taattttcag acaaatacag
     5041 aaacacagtc agacagagac aacacagaac gatgctgcag cagacaagac gcgcggcgcg
     5101 gcttcggtcc caaaccgaaa gcaaaaattc agacggaggc gggaactgtt ttaggttctc
     5161 gtctcctacc agaaccacat atccctccta ttaggggggt gcaccaaaga gtccaaaacg
     5221 atcgggattt ttggactcag gtcgggccac aaaaacggcc cccgaagtcc ctgggacgtc
     5281 tcccagggtt gcggccgggt gttccgaact cgtcagttcc accacgggtc cgccagatac
     5341 agagctagtt agctaactag taccgacgca ggcgcataaa atcagtcata gacactagac
     5401 aatcggacag acacagataa gttgctggcc agcttacctc ccggtggtgg gtcggtggtc
     5461 cctgggcagg ggtctcccga tcccggacga gcccccaaat gaaagacccc cgctgacggg
     5521 tagtcaatca ctcagaggag accctcccaa ggaacagcga gaccacaagt cggatgcaac
     5581 tgcaagaggg tttattggat acacgggtac ccgggcgact cagtcaatcg gaggactggc
     5641 gcgccgagtg aggggttgtg ggctctttta ttgagctcgg ggagcagaag cgcgcgaaca
     5701 gaagcgagaa gcgaactgat tggttagttc aaataaggca cagggtcatt tcaggtcctt
     5761 ggggcaccct ggaaacatct gatggttctc tagaaactgc tgagggctgg accgcatctg
     5821 gggaccatct gttcttggcc ctgagccggg gcaggaactg cttaccacag atatcctgtt
     5881 tggcccatat tcagctgttc catctgttct tggccctgag ccggggcagg aactgcttac
     5941 cacagatatc ctgtttggcc catattcagc tgttccatct gttcctgacc ttgatctgaa
     6001 cttttctatt ctcagttatg tatttttcca tgccttgcaa aatggcgtta cttaagctag
     6061 cttgccaaac ctacaggtgg ggtctttcat tatgcaggtg gcacttttcg gggaaatgtg
     6121 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     6181 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     6241 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     6301 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtg
//
