LOCUS       sequence-463036-       13004 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Generation of overexpressing Arabidopsis thaliana and Brassica napus
            lines to characterize the role of PbGH3 in plant..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13004)
  TITLE     A PBS3-like Gretchen Hagen 3 effector from the clubroot pathogen 
            modulates hormonal crosstalk to alter root morphology during 
            colonization
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pMDC32_PbGH3
FEATURES             Location/Qualifiers
     source          1..13004
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    775..915
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(841..860)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1001..1023
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      1259..1453
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(1519..2592)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(3021..3650)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    4951..4975
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     5163..5185
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5177..5194
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5178..5194
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5620..5964
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    6024..6048
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7753..7777)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      7841..8093
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(8110..8126)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8110..8126)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8123..8145)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8134..8150
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8158..8164,8165..8182,8183..8188))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8203..8224
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8415..9091
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             9158..10183
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    10224..10398
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    10476..10500
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             10925..11719
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
      841 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
      901 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
      961 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     1021 aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg
     1081 gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc
     1141 catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg
     1201 cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta
     1261 tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa
     1321 atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg
     1381 ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta
     1441 cgtgctatcc acaggaaaga gaccttttcg acctttttcc cctgctaggg caatttgccc
     1501 tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta
     1561 gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag
     1621 gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct
     1681 gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg
     1741 gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg
     1801 aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag
     1861 ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat
     1921 caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg
     1981 catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt
     2041 tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg
     2101 gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac
     2161 cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac
     2221 ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt
     2281 cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata
     2341 gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg
     2401 cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca
     2461 cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt
     2521 caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt
     2581 gcgaccgctc acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag
     2641 gcaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg
     2701 gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat
     2761 ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag
     2821 ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc
     2881 cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc
     2941 agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat
     3001 tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg
     3061 acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca
     3121 gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg
     3181 gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca
     3241 aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg
     3301 tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct
     3361 gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag
     3421 tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg
     3481 gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa
     3541 tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg
     3601 gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct
     3661 tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt
     3721 tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc
     3781 tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca
     3841 cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga
     3901 tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc
     3961 ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg
     4021 gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg
     4081 gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg tgcggccttc
     4141 ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc
     4201 aaacttcacg cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt cgatgattag
     4261 ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc cggccggcgt
     4321 ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct cctggatgcg
     4381 ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt
     4441 cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct
     4501 ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg
     4561 ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc
     4621 ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg
     4681 actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac
     4741 ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac
     4801 tgcactatag cagcggaggg gttggatcaa agtactttga tcccgagggg aaccctgtgg
     4861 ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt taaatatccg
     4921 attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac
     4981 tgatagttta aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca
     5041 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     5101 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     5161 ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgcatgcc tgcaggtcaa
     5221 catggtggag cacgacacac ttgtctactc caaaaatatc aaagatacag tctcagaaga
     5281 ccaaagggca attgagactt ttcaacaaag ggtaatatcc ggaaacctcc tcggattcca
     5341 ttgcccagct atctgtcact ttattgtgaa gatagtggaa aaggaaggtg gctcctacaa
     5401 atgccatcat tgcgataaag gaaaggccat cgttgaagat gcctctgccg acagtggtcc
     5461 caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc
     5521 ttcaaagcaa gtggattgat gtgataacat ggtggagcac gacacacttg tctactccaa
     5581 aaatatcaaa gatacagtct cagaagacca aagggcaatt gagacttttc aacaaagggt
     5641 aatatccgga aacctcctcg gattccattg cccagctatc tgtcacttta ttgtgaagat
     5701 agtggaaaag gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggccatcgt
     5761 tgaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt
     5821 ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac
     5881 tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg
     5941 aagttcattt catttggaga ggacctcgac tctagaggat ccccgggtac cgggcccccc
     6001 ctcgaggcgc gccaagctat caaacaagtt tgtacaaaaa agcaggctgc accatggcga
     6061 cgggggaggg gcactcgccg acgctggcga agctggcgag cggtgtcgag gaggcgaaca
     6121 gcgcggcact ggcaagcatc ctggggcgga acgcgcgcac gacgcaggtg ggtcgcgatt
     6181 tcgggttgga cccgacgtgg tcgccggacg cgttccgcga gaagtgcccg ctgacgacgt
     6241 atgccgacta cgagccgtac gcgaaccgga tgctggagag tgctgatccg tcgaatatca
     6301 tggtgccggg cgtgcccctc ttcatggcca cgtctggggg aacgacgggc gcgcggagca
     6361 aatgcttccc gatcacaggt cggacaagcc gggcggaacg cgtgcgccta tccctgatca
     6421 acgagtcgtt gccgtacgac gaatggaaag ggccatggtt gccgatgctg tcgtcccgta
     6481 tcgaccgtta tgcggcgtcg gggatgccag tctgcatact gtcgacgggg atgatggcgc
     6541 tgaccaatcg cctggacgca tcgttcgcga cgagtgtcgt cccgcgcccg gtgcacaacc
     6601 tggcgaacct gtcggaccac ttgaagctct acctgctgtt tgcgttgccg cggcgcgacc
     6661 tgaacgtcat catcacgacg ttcgggaacc tcgtcatcga cctgttccga tgcctgctgg
     6721 agaacaagga cgcgttcgtt gcggcgatcg ccgaaggcga actccccggg gacgagttct
     6781 tccaggacgc gctgccagat gacgttcgcc agcagctaca aagcgagttc gttcgcaacc
     6841 aaggccgcgc cgacgagctg gagtgcattt tcagcagcta tggcgaagat caccgagggc
     6901 tcattgcagc catatggccc agcttgcgcg tcgtcttcac cagtgtcaca ggcgcgttcg
     6961 agatgtaccg cgccccaatc caggacttcc ttggcgatgc cgtcaccatc atctcctttg
     7021 ggtatgccag cagcgagggc gagttcatcg ggataccaac gttccccaaa gatggcgttc
     7081 agaattacgt gttcgccccc ggcagcctgt tcctcgagtt catcagggat gaggatcggc
     7141 acctggctca gccgcccacg tgcctgctgt ccgagttgtg tcccgggcag tcgtacgaac
     7201 tggtgctgac caacgaacgc ggcctgtacc ggtaccgtac gtttgattcc atcgaagtcg
     7261 tggacatggt ccatggtgcg cttcccgtgt tcaagatcct tggccgggcc aatgtcgggt
     7321 tccgccttgt cggcggcgac gaattcatcg tggcggaatc cgagttgctt ccggcaatcg
     7381 tgaatgccgt ttcagagttg ccaggcacca tccccgagtt cggctcgtac ttggacgtcg
     7441 acgtgctccc cggccacatt gttgttgcca ttgagatgcc ggttggcgcc actccagatg
     7501 agcagctagc cgcgggcctg gccgagaaga tcgacgcgtc cctgtcgaca gcgaatccgc
     7561 gttaccgagc ctccatcacc gatggtcgcc tgtcgcccct ggccgtcgtt ttcgtttcgc
     7621 cgggagctct gtcccgcctt cgcgagttca agcagaagcg ggcgaacgtg gcgttctccc
     7681 aagtgaagct gccgctggcg ctcacagaca ccgccctcgt caacatactc aagaaccaga
     7741 ttgtgctgta ggacccagct ttcttgtaca aagtggttcg ataattctta attaactagt
     7801 tctagagcgg ccgccaccgc ggtggagctc gaatttcccc gatcgttcaa acatttggca
     7861 ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct
     7921 gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg
     7981 ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata
     8041 gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggaatt
     8101 cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     8161 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     8221 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     8281 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attggctaga gcagcttgcc
     8341 aacatggtgg agcacgacac tctcgtctac tccaagaata tcaaagatac agtctcagaa
     8401 gaccaaaggg ctattgagac ttttcaacaa agggtaatat cgggaaacct cctcggattc
     8461 cattgcccag ctatctgtca cttcatcaaa aggacagtag aaaaggaagg tggcacctac
     8521 aaatgccatc attgcgataa aggaaaggct atcgttcaag atgcctctgc cgacagtggt
     8581 cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg
     8641 tcttcaaagc aagtggattg atgtgaacat ggtggagcac gacactctcg tctactccaa
     8701 gaatatcaaa gatacagtct cagaagacca aagggctatt gagacttttc aacaaagggt
     8761 aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca tcaaaaggac
     8821 agtagaaaag gaaggtggca cctacaaatg ccatcattgc gataaaggaa aggctatcgt
     8881 tcaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt
     8941 ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac
     9001 tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg
     9061 aagttcattt catttggaga ggacacgctg aaatcaccag tctctctcta caaatctatc
     9121 tctctcgagc tttcgcagat ccggggggca atgagatatg aaaaagcctg aactcaccgc
     9181 gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct
     9241 ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct
     9301 gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc
     9361 atcggccgcg ctcccgattc cggaagtgct tgacattggg gagtttagcg agagcctgac
     9421 ctattgcatc tcccgccgtt cacagggtgt cacgttgcaa gacctgcctg aaaccgaact
     9481 gcccgctgtt ctacaaccgg tcgcggaggc tatggatgcg atcgctgcgg ccgatcttag
     9541 ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg
     9601 tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga
     9661 caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg
     9721 ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa
     9781 tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga
     9841 ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta
     9901 cttcgagcgg aggcatccgg agcttgcagg atcgccacga ctccgggcgt atatgctccg
     9961 cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg
    10021 ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca
    10081 aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag
    10141 tggaaaccga cgccccagca ctcgtccgag ggcaaagaaa tagagtagat gccgaccggg
    10201 atctgtcgat cgacaagctc gagtttctcc ataataatgt gtgagtagtt cccagataag
    10261 ggaattaggg ttcctatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat
    10321 gtatttgtat ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc
    10381 agtactaaaa tccagatccc ccgaattaat tcggcgttaa ttcagtacat taaaaacgtc
    10441 cgcaatgtgt tattaagttg tctaagcgtc aatttgttta caccacaata tatcctgcca
    10501 ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc
    10561 agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc
    10621 atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga
    10681 tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcacc
    10741 gcggtttcaa aatcggctcc gtcgatacta tgttatacgc caactttgaa aacaactttg
    10801 aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg
    10861 tcttgttata attagcttct tggggtatct ttaaatactg tagaaaagag gaaggaaata
    10921 ataaatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg
    10981 cgtaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg gagaaaatga
    11041 aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg atgtggaacg
    11101 ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg tcctgcactt
    11161 tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg tcctttgctc
    11221 ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg cggagtgcat
    11281 caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct tagacagccg
    11341 cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt gcgaaaactg
    11401 ggaagaagac actccattta aagatccgcg cgagctgtat gattttttaa agacggaaaa
    11461 gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca tctttgtgaa
    11521 agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg acaagtggta
    11581 tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac agtatgtcga
    11641 gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat attatatttt
    11701 actggatgaa ttgttttagt acctagggaa ggtgcgaata agcggggaaa ttcttctcgg
    11761 ctgactcagt catttcattt cttcatgttt gagccgattt tttctcccgt aaatgccttg
    11821 aatcagccta tttagaccgt ttcttcgcca tttaaggcgt tatccccagt ttttagtgag
    11881 atctctccca ctgacgtatc atttggtccg cccgaaacag gttggccagc gtgaataaca
    11941 tcgccagttg gttatcgttt ttcagcaacc ccttgtatct ggctttcacg aagccgaact
    12001 gtcgcttgat gatgcgaaat gggtgctcca ccctggcccg gatgctggct ttcatgtatt
    12061 cgatgttgat ggccgttttg ttcttgcgtg gatgctgttt caaggttctt accttgccgg
    12121 ggcgctcggc gatcagccag tccacatcca cctcggccag ctcctcgcgc tgtggcgccc
    12181 cttggtagcc ggcatcggct gagacaaatt gctcctctcc atgcagcaga ttacccagct
    12241 gattgaggtc atgctcgttg gccgcggtgg tgaccaggct gtgggtcagg ccactcttgg
    12301 catcgacacc aatgtgggcc ttcatgccaa agtgccactg attgcctttc ttggtctgat
    12361 gcatctccgg atcgcgttgc tgctctttgt tcttggtcga gctgggtgcc tcaatgatgg
    12421 tggcatcgac caaggtgcct tgagtcatca tgacgcctgc ttcggccagc cagcgattga
    12481 tggtcttgaa caattggcgg gccagttgat gctgctccag caggtggcgg aaattcatga
    12541 tggtggtgcg gtccggcaag gcgctatcca gggataaccg ggcaaacaga cgcatggagg
    12601 cgatttcgta cagagcatct tccatcgcgc catcgctcag gttgtaccaa tgctgcatgc
    12661 agtgaatgcg tagcatggtt tccagcggat aaggtcgccg gccattacca gccttggggt
    12721 aaaacggctc gatgacttcc accatgtttt gccatggcag aatctgctcc atgcgggaca
    12781 agaaaatctc ttttctggtc tgacggcgct tactgctgaa ttcactgtcg gcgaaggtaa
    12841 gttgatgact catgatgaac cctgttctat ggctccagat gacaaacatg atctcatatc
    12901 agggacttgt tcgcaccttc cctagaatgc atgaccaaaa tcccttaacg tgagttttcg
    12961 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttc
//
